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Citations for Agilent :
251 - 300 of 350 citations for 6 decenoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Cell proliferation rates were measured with label-free high-contrast brightfield microscopy in 6-well plates using Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Agilent) as described previously 58 ...
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bioRxiv - Physiology 2024Quote: ... MLO-Y4 cells with Pparγ silenced with siRNA (γY4KD) and their scrambled control (Scrl) were plated 6 hrs before the assay onto Seahorse cell culture microplates (Agilent Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Different FMv2-MDM4 versions containing mutations or deletions of single or combined L22 consensus sequences in MDM4 intron 6 were generated by QuikChange site-directed mutagenesis (200516, Agilent).
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bioRxiv - Microbiology 2023Quote: ... The sample was passed up and down through a 29-gauge needle 6-8 times and the fragment size distribution was determined (∼30 kbp; TapeStation, Agilent).
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bioRxiv - Immunology 2023Quote: ... antigen retrieval using sodium citrate buffer (10 mM, pH 6) was performed for 15 min at 110 °C in a DAKO Pascal pressure chamber (Agilent). After blocking in PBS supplemented with 10% goat serum (Sigma Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were loaded on a Superose 6 Increase column (Cytiva) run by a 1260 Infinity II HPLC (Agilent Technologies) at 0.6 mL/min ...
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bioRxiv - Microbiology 2019Quote: ... fumaric acid) were quantified in filtered culture supernatants (Costar® Spin-X® 0.22 μm) using isocratic high-performance liquid chromatography (Agilent 1260 Infinity series ...
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bioRxiv - Developmental Biology 2021Quote: ... The biodiesel obtained was stored and the amount of fatty acid methyl esters (FAMEs) were determined using a gas chromatography mass spectrometer (GCMS)(Agilent systems). The conditions used include hydrogen as the carrier gas with a flow rate of 40ml/minutes ...
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bioRxiv - Immunology 2022Quote: ... samples were acidified with formic acid (FA) and subsequently peptides were desalted using AssayMap C18 cartridges mounted on an AssayMap Bravo Platform (Agilent Technologies). Two biological replicates were pooled together to generate 100 micrograms of total peptide for TMT labeling and subsequent fractionation and phosphopeptide enrichment ...
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bioRxiv - Molecular Biology 2019Quote: ... plasmid was used and the mutations in amino acid position 339 of KLF1 were introduced by site directed mutagenesis (Agilent Technologies).
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bioRxiv - Cancer Biology 2020Quote: ... Sample pH was then adjusted to ~4 with formic acid prior to loading on Bond Elut-PH SPE columns (Agilent, 14102005), washing with aqueous buffer (30 mM ascorbic acid ...
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bioRxiv - Neuroscience 2020Quote: ... Primary myotubes underwent assessment of key parameters of glucose and fatty acid oxidation dependency and capacity using the Seahorse Mito Fuel Flex Test Kit (Agilent Technologies).
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bioRxiv - Neuroscience 2020Quote: The dependence and capacity of EDL fibers to use glucose and fatty acid as fuel substrates was determined using the Seahorse XF Mito Fuel Flex Test Kit (Agilent Technologies). Prior to the assay ...
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bioRxiv - Microbiology 2020Quote: Fatty acid methyl esters were obtained as previously described [17] and separated by using a gas chromatograph (model 6890 N; Agilent Technologies). Peaks were automatically computed and assigned using the Microbial Identification software package (MIDI) ...
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bioRxiv - Microbiology 2021Quote: ... and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 12 μM medronic acid (InfinityLab Deactivator additive, Agilent Technologies). The flow rate was kept at 250 μl/ml consisting of a 2 minutes hold at 10% B ...
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bioRxiv - Immunology 2021Quote: Elemental quantification on acid-digested samples was performed using an Agilent 7700 inductively coupled plasma mass spectrometer (Agilent, Santa Clara, CA) attached to a Teledyne CETAC Technologies ASX-560 autosampler (Teledyne CETAC Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... Photorespiration-related organic acids other than glyoxylate were quantified using a GC-MS system (7890B/5977A; Agilent Technologies, Santa Clara, CA) equipped with a DB-5MS capillary column (30 m ...
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bioRxiv - Microbiology 2021Quote: ... and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive, Agilent Technologies). The flow rate kept at 250 μl/ml consisting of a 2 minutes hold at 10% B ...
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bioRxiv - Biophysics 2019Quote: ... The fusion constructs were generated by deletion of amino acids from the 5-HT3A-ICD using appropriate partially overlapping primers with the QuikChange II Site-Directed Mutagenesis kit (Agilent Technologies) and were confirmed by DNA sequencing (GENEWIZ ...
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bioRxiv - Cancer Biology 2020Quote: ... Total PLs were separated by thin layer chromatography and the fatty acid content was measured by gas-liquid chromatography (GLC 7890A, Agilent Technologies) on a CP-Sil 88 100 m column (Agilent Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... amino acid substitutions were introduced using a QuikChange II XL site directed mutagenesis kit (200521) purchased from Agilent (Santa Clara, CA). After transformation into BL21-Gold (DE3 ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies were directed against: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), epithelial membrane antigen (EMA ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies were directed against: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), epithelial membrane antigen (EMA ...
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bioRxiv - Bioengineering 2021Quote: ... were extracted from freeze-dried sludge samples with a 3 h digestion time and 3% sulfuric acid and then analyzed by a gas chromatography-mass spectrometry (GC-MS) (Agilent, USA) with 7890A-5975C model (Lanham et al. ...
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bioRxiv - Microbiology 2021Quote: Short-chain fatty acids were analyzed by gas chromatography with a flame ionization detector (GC-FID 7890 A Agilent Technologies Inc.) with a fused silica capillary column (Nukon SUPELCO ...
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bioRxiv - Microbiology 2022Quote: ... and acetic acid) were quantified in filtered culture supernatants (Costar® Spin-X® 0.22 μm) using isocratic high-performance liquid chromatography (Agilent 1260 Infinity series ...
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bioRxiv - Microbiology 2022Quote: ... and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive, Agilent Technologies). The flow rate was kept at 250 μL mL−1 consisting of a 2 min hold at 10% B ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids harboring GlnR amino acid substitutions (pET28a-glnR derivatives) were constructed using site-directed mutagenesis (QuikChange Site-Directed Mutagenesis Kit, Agilent, Inc.).DNA fragments consisting of different canonical GlnR-dependent promoters (narG whose downstream genes encoding M ...
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bioRxiv - Cancer Biology 2023Quote: ... Sample pH was then adjusted to 4 with 15 μL of 20% formic acid before loading onto conditioned SPE Bond-Elute pH columns (Agilent, 14102062) at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... 20 µl 20% formic acid was added to lower the pH and samples were cleaned on Bond Elut OMIX C18 pipette tips (Agilent Technologies) following the manufacturer’s instruction.
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bioRxiv - Bioengineering 2023Quote: ... PHA was extracted from lyophilized sludge using acidified methanol (3% sulfuric acid) containing chloroform and analyzed using a gas chromatography-mass spectrometry (Agilent, USA). Glycogen in sludge was extracted in a similar way but using 0.9M HCl and analyzed using a liquid chromatography-mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... the bile acids identified were analyzed by Mass Hunter Qualitative 10.0 qualitative (Version B.10.0, Agilent software metabolomics, Agilent Technologies, Waldbronn, Germany).
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bioRxiv - Systems Biology 2023Quote: ... bile acids and short chain fatty acids analyses were performed by LC-MS/MS with a 1260 UHPLC (Ultra-High Performance Liquid Chromatography) (Agilent Technologies) coupled to a QQQ 6410 (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... The digestion was quenched with 10% formic acid (FA) and the resulting peptides were cleaned-up in an automated fashion using the AssayMap Bravo platform (Agilent Technologies) with a corresponding AssayMap C18 reverse-phase column ...
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bioRxiv - Molecular Biology 2024Quote: ... After irradiation the sample was combined with 10 µl of formic acid 1 % and 10 µl analyzed by LC-MS using an Ultimate 1100 HPLC system (Agilent Technologies) connected to an Amazon Speed ETD ion-trap mass spectrometer (Bruker Daltonics) ...
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bioRxiv - Cell Biology 2020Quote: ... acidified by nitric acid before the measurement of heavy metals by Inductively Coupled Plasma Optical Emission Spectrometer (Agilent ICP-OES 5110, America). The test methods were carried out according to standard protocol(Method of determination for 26 elements (copper ...
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bioRxiv - Evolutionary Biology 2021Quote: ... segetum to the synthetic mixture of C6-C10 straight chain acids were recorded on an Agilent 7890 gas chromatograph equipped with a flame ionization detector (FID) (Agilent Technologies, USA) and an electroantennographic detector (EAD ...
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bioRxiv - Neuroscience 2019Quote: ... Paraffin embedded human brain sections were de-waxed for antigen retrieval with 90% formic acid (5 min) followed by citrate boiling (45min, 98°C DAKO Citrate Buffer, DAKO PT Link). Sections were then blocked using 0.1% (w/v ...
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bioRxiv - Cell Biology 2019Quote: ... Point mutations for amino acid substitutions or truncations were generated using the QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA).
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bioRxiv - Biochemistry 2019Quote: ... Amino acids were then measured by automated pre-column o-Phthaldialdehyde derivatization using HPLC (1290 Infinity LC system, Agilent Technologies Pvt. Ltd). The mobile phases consisted of 10 mM Na2HPO4 and 10mM Na2B4O7 (Buffer A ...
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bioRxiv - Bioengineering 2021Quote: ... pCS2-GemininΔ27-GFP was generated by deleting amino acids 1-27 from Geminin-GFP [27] using QuikChange® Lightning mutagenesis kit (Agilent, 210513). The plasmids pCS2-Flag-FA-Geminin-GFP w.t and Δ27 variant were generated by cloning Geminin-GFP into pCS2-Flag-FA vector sing FseI and AscI restriction sites ...
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bioRxiv - Systems Biology 2021Quote: ... detected with a diode array detector (DAD G7117A) and quantified following manufacturer’s guidelines (AdvanceBio Amino Acid Analysis, © Agilent Technologies, Inc. 2018). Standards ranging from 5 μM to 30 mM were used for the quantification of aspartate ...
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bioRxiv - Microbiology 2020Quote: Caprylic acid concentration was quantified by RP-HPLC following Herrera et al.37 using a liquid chromatographer (1220, Agilent Technologies; California, USA) equipped with an Eclipse XDB-C8 5 μm column (150 mm × 4.6 mm i.d.) ...
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bioRxiv - Cell Biology 2021Quote: ... The upper ether layer was collected and SCFA content was analyzed on an Agilent 7890B gas chromatography system with flame ionization detector using a high-resolution capillary column for detection of volatile acids (DB-FFAP, 30 m x 0.25 mm with 0.25 μm film thickness; Agilent Technologies, Santa Clara, CA). A standard solution containing 10 mM of acetic ...
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bioRxiv - Plant Biology 2020Quote: ... the HY5 36th Serine (AGC) was changed into Alanine (GCC) and Aspartic Acid (GAC) respectively using Quickchange II site-directed mutagenesis kit (Agilent, Catalog #200523) and cloned into pB7FWG vectors ...
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bioRxiv - Biochemistry 2021Quote: ... The digestion and desalting (total time 3 min) were driven by 0.4% formic acid (FA) in water pumped by 1260 Infinity II Quaternary pump (Agilent Technologies, Waldbronn, Germany) at a flow rate of 100 μL·min−1 ...
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bioRxiv - Microbiology 2020Quote: Each ELV and MV preparation were reconstituted in 10 μl of 5% formic acid and injected onto a 50 mm 300 μm C18 trap column (Agilent Technologies, USA). The samples were then desalted for 5 min at 30 μl/min using 0.1% formic acid and the peptides were then eluted onto an analytical nano-HPLC column (150 mm × 75 μm 300SBC18 ...
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bioRxiv - Cancer Biology 2020Quote: ... The concentration of total RNA was measured on a Nanodrop device and the quality of the extracted nucleic acid was assessed using a Bio-analyzer 2100 (Agilent technologies, CA). Microarray probes were synthetized in one cycle of RNA amplification in which molecules were labeled (Affymetrix microarray station ...
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bioRxiv - Cancer Biology 2020Quote: ... Concentration of total RNA was measured using a Nanodrop spectrophotometer and the quality of the extracted nucleic acid was assessed on a Bio-analyzer 2100 (Agilent Technologies, CA). Microarray probes was synthetized by one cycle of RNA amplification in which molecules were labeled in an Affymetrix microarray station (Affymetrix ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were resuspended in hexane and fatty acids were separated with gas chromatography (8860 or 7890A GC system, Agilent Technologies, CA, USA) combined with mass spectrometry (5977B or 5975C Inert MS system ...