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Citations for Agilent :
2701 - 2750 of 5728 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: The AAV plasmid of human synapsin I promoter (hSyn)-tetracycline-controlled transactivator 2 (tTA2) was constructed by subcloning the DNA fragments containing hSyn and tTA2 into pAAV-MCS (Agilent Technologies, CA, USA). The AAV vector was produced as described previously (Ebina et al. ...
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bioRxiv - Microbiology 2023Quote: ... Plate was shaken continuously at 30°C and OD600 was read every 20 minutes in a BioTek Epoch 2 Microplate Spectrophotometer (Agilent, Santa Clara, CA). MMC (0.5 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... The AAV plasmid of human synapsin I promoter (hSyn)-tetracycline-controlled transactivator 2 (tTA2) was constructed by subcloning the DNA fragments containing hSyn and tTA2 into pAAV-MCS (Agilent Technologies, CA, USA). The AAV vector was produced as described previously15,35,55 ...
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Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... Coverslips were then incubated with 1X DAPI for 5 min before mounting on slides using DAKO mounting media (Agilent Technologies, Cat #S302380-2). Images were captured at 60X or 100X using a Nikon C2+ confocal microscope coupled to NIS-Elements AR software (Nikon).
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bioRxiv - Pathology 2024Quote: The samples were resuspended in 10µL phase A of Q-TOF (2% ACN, 0.1% FA in water HPLC grade) in mass spectrometry polypropylene vials (Agilent technologies, reference 5190-3155). The samples (1µL out of 10µL ...
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bioRxiv - Biochemistry 2024Quote: ... Assay plates were generated by plating 2 µL of the deep well stock plates into 384-well assay plates (Thermo Scientific #142761 using the Agilent V11 Bravo). 88 µL of SrEpac cultured in high-nutrient media (5% Sea Water Complete ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-E-cadherin (diluted 1:100) antibody (Ab) and anti-vimentin Ab (1:1000) were purchased from DakoCytomation, mouse anti-p65 Ab (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... then followed by immunoblotting for ≤ 1 h with secondary antibodies: 1:5,000 anti-mouse linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Microbiology 2022Quote: ... then followed by immunoblotting for ≤ 1 h with secondary antibodies: 1:5,000 anti-mouse linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Immunology 2020Quote: ... followed by the incubation with horseradish peroxidase-labeled secondary antibody (1:10,000) for 1 h at room temperature (DAKO EnVision™+ System ...
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bioRxiv - Neuroscience 2021Quote: ... WAKO) antibodies were diluted at 1:250 and 1:1000 respectively with EnVision FLEX Antibody Diluent (K800621, Dako-Agilent) and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... WAKO) antibodies were diluted at 1:250 and 1:1000 respectively with EnVision FLEX Antibody Diluent (K800621, Dako-Agilent) and incubated overnight at 4°C ...
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bioRxiv - Genetics 2019Quote: ... the following primary antibodies were used at a 1:100 dilution: 1) anti-GH rabbit polyclonal antibody (Dako, A0570); 2 ...
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bioRxiv - Pathology 2020Quote: ... It was then incubated with horseradish peroxidase-labelled secondary antibodies (cat. no. sc-2020; 1:5,000; Santa Cruz Biotechnology, Inc.; and cat. no. K4003; 1:5,000; Dako; Agilent Technologies ...
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bioRxiv - Pathology 2020Quote: ... It was then incubated with horseradish peroxidase-labelled secondary antibodies (cat. no. sc-2020; 1:5,000; Santa Cruz Biotechnology, Inc.; and cat. no. K4003; 1:5,000; Dako; Agilent Technologies ...
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bioRxiv - Genetics 2020Quote: ... We determined efficiency of each reaction using the equation Efficiency = −1+10(−1/slope of standard curve) (Agilent Genomics). We additionally calculated ratios of transcript abundance to determine expression levels of one gene relative to another gene and to account for any differences in underlying mRNA levels among individuals ...
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bioRxiv - Neuroscience 2022Quote: ... The following day slides were rinsed and incubated with horseradish peroxidase labelled secondary antibodies for 1 hour and developed using 3,3′-diaminobenzidine (DAB, 1:50, DAKO) for 5 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... was followed by incubation with streptavidin conjugated to horseradish peroxidase (1:200; 1.25% NGS/1% BSA in PBS; 30 min) (#P0397, DakoCytomation). For visualization DAB (#D5905 ...
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bioRxiv - Neuroscience 2024Quote: ... anti-Gfap rabbit polyclonal antibody (1:2,000 [brain] or 1:4,000 [spinal cord]; Z0334; Dako/Agilent, Santa Clara, CA); and anti-Iba1 rabbit polyclonal antibody (1:2,000 [brain] or 1:4,000 [spinal cord] ...
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bioRxiv - Neuroscience 2024Quote: ... anti-Gfap rabbit polyclonal antibody (1:2,000 [brain] or 1:4,000 [spinal cord]; Z0334; Dako/Agilent, Santa Clara, CA); and anti-Iba1 rabbit polyclonal antibody (1:2,000 [brain] or 1:4,000 [spinal cord] ...
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bioRxiv - Neuroscience 2023Quote: ... followed by washing in TBST (0.1%) and a 1 h incubation in secondary antibody (goat a-mouse-HRP: 1:10000, P0447, Dako). After washing ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating brain slices were incubated in a solution of anti-Iba1 antibody (1:2000; Fuji film-Wako) or anti-GFAP antibody (1:10,000; Agilent-Dako) at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... prior to incubation in primary antibodies (anti-YKL-40, Abcam ab77528, 1:1,000; anti-C3d, Agilent A0063, 1:1,000). Sections were incubated in primary antibodies for one hour at room temperature prior to washing and probing with the universal probe and polymer-HRP components of the Mach 4 Polymer kits (CellPath ...
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bioRxiv - Systems Biology 2024Quote: ... for 1 h and washed in PBS before being incubated with anti-rat biotinylated secondary antibody (Dako, 1:100) for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... PSC27 was seeded at a density of 5 × 104 cells/ well in the XF24 cell culture microplate (Agilent Technologies, 04721 and Q01321) at a 37[5% CO2 incubator for overnight ...
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bioRxiv - Cell Biology 2022Quote: ... HAP1 cells and SH-SY5Y cells were seeded at a density of 40,000 cells/well and HEK293 cells were seeded at a density of 20,000 cells/well on Seahorse XF96 V3-PS Microplates (Agilent Technologies, 101085-004) after being trypsinized and counted (Bio-Rad TC20 automated Cell Counter ...
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bioRxiv - Neuroscience 2024Quote: ... fibroblasts were seeded at 45 × 103 cells per well (5-10 replicates per cell line) in fibroblast growth media in a Seahorse XFe24 Microplate (Agilent, 100777-004) and incubated for 24 hours to allow cells to adhere ...
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bioRxiv - Cancer Biology 2021Quote: ... and XF Cell Mito Stress Test Kit (Seahorse Bioscience). Cells were seeded at 50,000 cells/well (~80-90% confluent when assayed ...
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bioRxiv - Biochemistry 2020Quote: ... ctXPD was expressed in ArcticExpress (DE3) RIL cells (Agilent). Ctp62/ctp44 ...
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bioRxiv - Cancer Biology 2021Quote: ... The XF Cell Mito Stress Test Kit (Agilent, 103015), was used for the assay ...
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bioRxiv - Cell Biology 2022Quote: BL21-CodonPlus (DE3)-RIL competent cells (Agilent Technologies 230245) were transformed with Cas9 plasmid (Addgene #67881) ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with phenol-free basal media (Agilent) and exposed to 150µL of assay media ...
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bioRxiv - Molecular Biology 2021Quote: ... coli TG1 cells (Agilent Technologies Japan, Ltd., Tokyo, Japan) were transformed with the ligated plasmids under chilled conditions (Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2019Quote: ... Following protein expression in BL21 (DE3) RIPL cells (Agilent), protein was purified by immobilized metal-affinity chromatography (IMAC ...
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bioRxiv - Cell Biology 2019Quote: ... was purified from BL21-DE3 RIPL cells (Stratagene 230280) as previously described (Khokhlatchev et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... For transformation into XL10-Gold ultracompetent cells (Agilent Technologies) the Gibson Assembly was diluted 1:4 ...
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bioRxiv - Synthetic Biology 2019Quote: ... and propagated in E.coli XL 10 Gold cells (Agilent). All cassettes contain Kozak sequences ...
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... coli BL21-CodonPlus (DE3) RIPL chemically competent cells (Agilent). EWS RBD methylation was achieved by cotransformation along with a glutathione S-transferase-linked protein arginine methyltransferase 1 (PRMT1 ...
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bioRxiv - Neuroscience 2021Quote: ... coli BL21 CodonPlus (DE3)-RIPL competent cells (Agilent, 230280) using auto-induction protocols (Studier ...
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bioRxiv - Plant Biology 2021Quote: ... coli XL10-Gold Ultracompetent Cells following manufacturer’s instructions (Agilent). The transformants were plated on LB-agar with ampicillin selection and grown overnight at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and expressed using BL21-CodonPlus (DE3)-RIPL cells (Agilent). All proteins were first purified using affinity chromatography on HisTrap column followed by HiTrap heparin purification in 250-1500 mM gradient of NaCl (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... Constructs were built in competent XL10-Gold cells (Agilent) and subsequently transformed in the appropriate strain for experiments.
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bioRxiv - Biochemistry 2022Quote: ... coli BL21-CodonPlus (DE3)-RIPL competent cells (Agilent Technologies). Briefly ...
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bioRxiv - Immunology 2022Quote: ... pre-coated XF96 Cell Culture Microplates (Seahorse, Agilent Technologies). Cells were centrifuged at 200 ×g for 2 min in a zero-braking setting to immobilize cells before being supplied with an additional 130 μL assay medium and kept in a non-CO2 incubator for 30 min ...
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bioRxiv - Immunology 2022Quote: ... and Seahorse XF Cell Mito Stress Test Kit (Agilent) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... Each construct was transformed into BL21(DE3) cells (Agilent), and expression was induced with 0.2 mM isopropyl-β-D-thio-galactoside (IPTG ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were plated into XF24 (Agilent; Cat#100867-100) or XF96 (Agilent ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were analyzed by flow cytometry (ACEA Novocyte, Agilent). Data was analyzed with FlowJo™ v10 software.
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bioRxiv - Plant Biology 2021Quote: ... coli ArcticExpress (DE3) cells (Agilent technologies, Santa Clara, USA) (from now on denoted as protocol 2 ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21 Gold CodonPlus® (DE3) RIL cells (Agilent), and 5-10 colonies were used to directly inoculate 100 mL cultures in LB medium supplemented with 100 μg/mL ampicillin or carbenicillin and 34 μg/mL chloramphenicol ...