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Citations for Agilent :
2701 - 2750 of 3242 citations for Recombinant Human Complement Component 5a Receptor 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Cell Biology 2024Quote: ... GC-FID was performed using an Agilent 7890 gas chromatograph equipped with a DB-WAX column (15 m x 0.53 mm, 1 µm; Agilent) and flame ionization detection ...
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bioRxiv - Synthetic Biology 2023Quote: ... An Agilent 7693A autosampler fitted with a 100 µL gastight syringe was used to inject headspace gas (50 µL) into a DB-VRX capillary column (20 m, 0.18 mm ID, 1 µm film; Agilent). Experiments were performed using a 55:1 split ratio ...
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bioRxiv - Microbiology 2023Quote: ... RNA integrity was assessed with 1% (m/v) agarose gel and Agilent 2100 Bioanalyzer System (Agilent Technologies, 5067-4626). For antibiotic treatment conditions ...
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bioRxiv - Immunology 2022Quote: ... The dpy-7p::old-1(D321A, N326S)::GFP plasmid (pFD23) was created using a Site Directed Mutagenesis Kit (Agilent) to introduce mutations into pFD21 using primers old-1kinasemutF and old-1kinasemutR ...
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bioRxiv - Developmental Biology 2023Quote: ... the beads were transferred into a 1-mL PP filtration microplate (Agilent, 7 µm frit, cat. no. 202501-100) and washed 3x with 200 µL 1x PBS and 2x 200 µL ultrapure water ...
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bioRxiv - Biophysics 2023Quote: Drp1 isoform 1 (Genbank accession number AB006965) was PCR amplified with Pfu Turbo DNA polymerase (Stratagene, La Jolla, CA) as an NdeI/XhoI fragment ...
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bioRxiv - Biochemistry 2023Quote: ... and m6A(1-100 mM, MCE HY-N0086) were analyzed on RapidFire 300 high-throughput SPE system (Agilent Technologies) interfaced with a Sciex 6500 (Sciex) ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary anti-mouse-HRP (Santacruz sc-516102 Lot#c1419) was diluted 1: 10,000 or anti-rabbit-HRP (Dako P0448) 1:1000 in blocking buffer and incubated for 1hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... RNA quality was measured by 1% agarose gel electrophoresis (Figure S3A) and by High Sensitivity (HS) DNA Assay (Agilent) (Figure S3B).
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed either in 1% citrate buffer or in DAKO antigen retrieval solution (S1699, DAKO Agilent, USA) at 97.5 °C.
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed either in 1% citrate buffer or in DAKO antigen retrieval solution (S1699, DAKO Agilent, USA) at 97.5 °C.
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR was performed using the 1-Step Brilliant II SYBR Green QRT-PCR Master Mix kit (Agilent Technologies) and the TaqPath 1-Step Multiplex Master Mix kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were also assessed for size distribution and adapter dimer presence (<0.5%) by the High Sensitivity NGS Fragment 1-6000bp kit on a 96-channel Fragment Analyzer (Agilent). All sequencing libraries were then normalized on the MicroLab STAR (Hamilton) ...
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bioRxiv - Cell Biology 2024Quote: ... and qualitatively assessed with the High Sensitivity NGS Fragment 1-6000bp Kit on a 96-channel Fragment Analyzer (Agilent). Final libraries were normalized ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then rinsed with PBS containing 1% FBS prior to imaging using a Cytation 5 instrument (Agilent BioTek). Mean fluorescence was calculated using Gen5 software (RRID:SCR_017317).
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bioRxiv - Synthetic Biology 2024Quote: ... was added with the estimation that OD600 of 1 is approximately equal to 8×108 CFU/ml (Agilent biocalculator). The MOI of 0.01 was administered to E ...
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bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). Oxygen consumption rates (OCR ...
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bioRxiv - Immunology 2024Quote: ... the slides were washed in 1× PBS and then exposed to Dako Serum-free Protein Block solution (Agilent, USA) for 45 min at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... The digest was filtered (0.22 μm) and filtered samples (1-2 μg) were loaded onto a C18 high capacity nano LC chip (Agilent Technologies) using a 1200 series capillary and eluted directly into a 6550 series quadrupole time-of-flight mass spectrometer (Agilent Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL of sample was injected into the GC (Sigma-Aldrich 5973 Network mass selective detector attached to an Agilent 6890 gas chromatograph (Agilent Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... Two µl of samples were introduced in the splitless injection mode in DB-5 (60 m × 0.25 mm, 1 µm film thickness, Agilent, CA) using helium as a carrier gas ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... or 1 µM (OMM1) carbonyl cyanide 4-(trifluoromethoxy)-phenylhydrazone (FCCP) and 0.5 µM of a rotenone antimycin A mix (Agilent Technologies). Following the assay ...
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bioRxiv - Cancer Biology 2021Quote: ... The plate was incubated in a CO2-free 37°C incubator for 1 hour and a Mitochondrial Stress Test (Agilent) was performed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... S34F or Q157R were treated with doxycycline (1 μg/mL) for 48 hours before UV-crosslinking (400 mJ/cm2 UV Stratalinker 2400, STRATAGENE). RNA-protein complexes were immunoprecipitated with 12 μg anti-FLAG M2 antibody (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... the specimens were detected by incubating with goat anti-rabbit or anti-rabbit/mouse IgG-HRP for 1 h and subsequently with DAB (Dako). The tissue sections were then counterstained with 0.1% (wt/vol ...
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bioRxiv - Cell Biology 2020Quote: ... ∼100 separate PCR reactions (library and representation dependent) were performed with 1 μg genomic DNA in each reaction using Herculase II Fusion DNA Polymerase (Agilent) or Phusion High-Fidelity DNA Polymerase (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... and the cDNA hybridised to an 8 Chamber Agilent slide at 65°C for 16 hours before washing the slide with Oligo aCGH Wash Buffer 1 (Agilent) for 5 minutes at room temperature and Oligo aCGH Wash Buffer 2 (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... The TIMS elution voltage was linearly calibrated to obtain 1/K0 ratios using three ions from the ESI-L Tuning Mix (Agilent) (m/z 622 ...
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bioRxiv - Molecular Biology 2021Quote: ... The TIMS elution voltage was linearly calibrated to obtain 1/K0 ratios using three ions from the ESI-L Tuning Mix (Agilent) (m/z 622 ...
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bioRxiv - Developmental Biology 2020Quote: ... then 1 ml of the headspace gas was withdrew and injected into a gas chromatograph (Agilent GC 6890N, CA, USA) fitted with a flame ionization detector and an activated alumina column (Zhang et al. ...
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bioRxiv - Neuroscience 2021Quote: ... We used additional microglial markers including mouse anti-MHC-II/HLA-DP/DQ/DR (1:250, M077501-2, Agilent, UK), rabbit anti-PU.1 (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... Size of chromatin fragments was verified on a 1% agarose gel and on a high sensitivity DNA chip in a 2100 bioanalyzer (Agilent) after decrosslinking overnight at 65°C with shaking in elution buffer (100 mM NaHCO3 ...
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bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated secondary antibodies were then incubated on slides for 1-hour and detected with the DAB+ chromogen kit (DAKO). After washing ...
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bioRxiv - Molecular Biology 2021Quote: Reverse transcription for PCR analysis was performed using 1 μg total RNA and 300 ng random hexamers using Affinity script multiple temperature reverse transcriptase (Agilent). For UTP11 ...
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bioRxiv - Immunology 2022Quote: ... for 1 hour RT and replacing the secondary antibody incubation with HRP-labeled polyclonal goat α-mouse antibody (Dako K4000) for 30 minutes RT ...
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bioRxiv - Immunology 2019Quote: ... overnight at 4 °C followed by a 1 h incubation with either an HRP-conjugated goat anti-mouse IgG or goat anti-rabbit IgG (Dako). Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore ...
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bioRxiv - Immunology 2019Quote: ... Immunofluorescence staining was carried out using the following antibodies: a primary rabbit anti-GFAP (resting and reactive astrocytes, 1:500 dilution, Z0334, Dako), and a primary rabbit anti-Iba1 (resting and activated microglia/macrophages ...
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bioRxiv - Plant Biology 2019Quote: ... Samples were then diluted 1:11 in ultrapure water before analysis with ICP-OES (Vista-PRO CCD Simultaneous ICP-OES; Agilent). Calibration was carried out using standards of Zn ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were incubated in the given primary antibody in a dilution of 1:1000 and appropriate HRP-conjugated secondary antibody (Dako) in a dilution of 1:1500 ...
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bioRxiv - Microbiology 2019Quote: ... The resulting products were then run with a Fragment Analyzer capillary electrophoresis using the DNF905 1-500 base pair kit (Agilent). Quantification was performed using the following equation ...
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bioRxiv - Molecular Biology 2019Quote: ... and the beads were resuspended in 50 μL Luminex block buffer containing a 1:5,000 dilution of streptavidin-phycoerythrin (Prozyme #PJ31S). The plate was shaken at 1100 RPM for 30 sec and incubated for 30 min at 600 RPM ...
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bioRxiv - Microbiology 2019Quote: UV-Visible spectra were recorded in 1 cm optic path quartz cuvette under aerobic conditions on a Cary 100 UV-Vis spectrophotometer (Agilent) and under anaerobic conditions in a glove box on a XL-100 Uvikon spectrophotometer equipped with optical fibers.
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bioRxiv - Cancer Biology 2019Quote: ... single strand cDNA synthesis and PCR amplification were carried out in a 1-step reaction using the Brilliant II QRT-PCR Master Mix (Agilent) and TaqMan gene expression assays (Life Technologies) ...
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bioRxiv - Cell Biology 2019Quote: HEK293T cells were seeded in 96-well plates (1×104 cells/well) (Thermoscientific, 136101) and transfected with pNF-κB-Luc (Stratagene) and phRK-TK (Renilla ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the CMV enhancer was amplified with the primers P322 and P323 using the phrGFPII-1 plasmid (Agilent Technologies, Santa Clara) as template ...
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bioRxiv - Neuroscience 2020Quote: ... IdU) on alternate one-in-ten sections using different anti-BrdU antibodies from different vendors (for BrdU: 1/200, Dako, Glostrup ...
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bioRxiv - Cell Biology 2021Quote: The 3a ORF from SARS CoV-1 (Tor2 strain) was originally obtained from the Institute for Genomic Research and subcloned into pBluescript (Stratagene) prior to transferring to pCAGGS-MCS [17] after PCR amplification with KpnI and XhoI restriction sites ...
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bioRxiv - Cell Biology 2021Quote: ... RNA concentration was measured by Nanodrop and 1 to 3 μg of RNA was reverse transcribed with AffinityScript Multi-Temp RT (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... and retrovirus expressing EGFP in alternating one-in ten sections using different anti-BrdU antibodies from different vendors (for BrdU: mouse primary at 1/100, Dako; CldU ...