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Citations for Agilent :
2651 - 2700 of 2870 citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: 50 μL of pure CrRPE1 concentrated at 9.5 mg mL−1 were injected on BioSEC-3 300 size-exclusion chromatography column (Agilent Technologies, Santa Clara, USA) equilibrated in buffer 20 mM Tris-HCl (pH 7.9 ...
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bioRxiv - Immunology 2022Quote: ... Quality control of the libraries to ensure no adapter dimers were present was carried out by examining 1ul of a 1:5 dilution on a High Sensitivity DNA chip (Agilent Technologies: 5067-4626) on an Agilent 2100 Bioanalyzer ...
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bioRxiv - Pathology 2022Quote: ... and a monoclonal antibody directed against the alpha isoform of smooth muscle actin at a working dilution of 1/100 (a-SMA, clone 1A4, n M0851; Dako, Denmark A/S). Alligator skeletal muscle was used as positive control for anti-desmin antibody (Supplemental figure 3A) ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight on a shaker at room temperature in fresh blocking solution with polyclonal rabbit anti-GFAP (1:1000, Dako, Santa Clara, CA). The following day tissue was washed in PBST and incubated for 2.5h at room temperature with Alexa Fluor 488 donkey anti-rabbit secondary (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... OCI-LY-1 cells (5 × 105 cells/ml) were pretreated for 1 hour in Seahorse Seahorse XF base medium supplemented with glutamine (103334-100, Agilent Technologies, Heverlee, Belgium) in a CO2-free incubator ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated 1 hour at room temperature (RT) with EnVision HRP system anti-mouse (K400111-2, Agilent Technologies, Santa Clara, CA, USA) in blocking solution ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated at 37°C in a non-CO2 incubator for 1 hour and subsequently loaded into the Seahorse XFe96 Analyzer (Agilent Technologies, Seahorse Bioscience) when prompted ...
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bioRxiv - Microbiology 2023Quote: ... tissues were incubated with proteinase K diluted 1:10 in 1X TBS (Fisher Scientific, Cat. #BP24711; and PNA ISH kit, Agilent Dako, Cat. #K5201) for 10 min at RT in a humidity chamber ...
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bioRxiv - Genomics 2023Quote: ... 0.9 μl of ‘11.1x buffer’ [21] and 0.06 μl of a 20:1 mix of PCRBIO Taq Polymerase (PCRBiosystems; 5U/μl): Pfu DNA polymerase (Agilent Technologies; 2.5 U/μl). PCR cycling conditions for the amplification of cat mitogenome segments generally consisted of an initial denaturation at 96°C for 1 min ...
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bioRxiv - Plant Biology 2023Quote: ... Peptides were dissolved in loading solvent A (0.1% TFA in water/ACN (98:2, v/v)) and desalted on a reversed-phase (RP) C18 OMIX tip (Agilent, Santa Clara, USA). The tip was first washed 3 times with 100 μl pre-wash buffer (0.1% TFA in water/ ACN (20:80 ...
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bioRxiv - Microbiology 2023Quote: ... The quality of the DNA was checked on a Nanodrop to ensure that the OD600 260/230 and 260/280 ratios were >1.8 and the DNA length distribution was checked using a Femto Pulse (Agilent Technologies; Supplementary Figure 1).
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Extracts were then evaporated to a volume of approximately 15 μL of which 1 μL was analyzed using a 6890 gas chromatograph (GC) coupled to a 5975 mass selective detector (MS) (Agilent Technologies, Waldbronn, Germany). The GC was equipped with a DB-5 capillary column (0.25 mm ID × 30 m ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were incubated for 1 hour with horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit antibodies (Agilent Technologies, Santa Clara, CA, USA), washed with PBS 0.05% Tween ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µL of individual polyribosomal RNAs were loaded on a PicoChip and run on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), according to the supplied protocol ...
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bioRxiv - Immunology 2024Quote: ... HER2 CAR-T cells or mock CAR-T cells from the same donor were added at an effector:target cell ratio of 10:1 and measured in a real-time cell analysis assay using the xCELLigence SP system (Agilent, Santa Clara, CA). An electrical current was applied to each co-culture ...
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bioRxiv - Plant Biology 2024Quote: ... The peptide-containing supernatant was subjected to solid phase extraction using SampliQ C18 cartridges (1 ml, 100 mg, 5982-1111, Agilent, Santa Clara, USA). Prior to the extraction the cartridge was washed successively with 1 ml 0.1% TFA,0.1% TFA in 80% ACN ...
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bioRxiv - Microbiology 2024Quote: ... Grids were then washed with PBS and blocked with 1% FBS for 5 min Grids were subsequently incubated with rabbit anti-CEA (Dako, supplemental table 2) for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with HRP-conjugated secondary antibody (diluted 1:2000 in Blocking buffer, rabbit α-mouse DAKO #0260, swine α-rabbit DAKO # P0217) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... the granulocytic protein CD15 (always absent in M-MDSCs given that it is observed in both mature neutrophils and the polymorphonuclear subset of MDSCs, 1:25, Agilent ISO62, carb3 clone) and the specific molecule of antigen presenting-cells HLA-DR (low or absent in M-MDSCs ...
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bioRxiv - Cancer Biology 2024Quote: ... and the combination of antimycin A/rotenone (1 µM) into the culture medium (Seahorse XF Cell Mito Stress Test Kit, Agilent Technologies, 103015-100). Glutaminolysis was performed by using respiration kit (Seahorse XF Cell Mito Stress Test Kit ...
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bioRxiv - Immunology 2024Quote: ... The antigens on the slides were retrieved by submerging slides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent, Cat# S2375) and incubating at 97 °C for 40 min in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed three times with TBS-T for 5 min and incubated for 1 h at room temperature with anti-rabbit immunoglobulin/HRP secondary antibody (Agilent, Santa Clara, CA) or with anti-mouse IgG-Peroxidase secondary antibody (MilliporeSigma) ...
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bioRxiv - Cancer Biology 2021Quote: ... and the cells were washed twice with 200 μL/well of assay medium (XF DMEM Base Medium, pH 7.4 containing 25 mM Glucose and 4 mM Glutamine; Agilent). After washing ...
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bioRxiv - Plant Biology 2021Quote: ... A total of 4 µL of each filtered sample was loaded onto a C18 high-capacity nanoLCchip (Agilent Technologies) using a 1200 series capillary pump (Agilent Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Gene expression analysis was conducted using Agilent Whole Mouse Genome 4×44 multiplex format oligo arrays (Agilent Technologies 014868) following the Agilent 1-color microarray-based gene expression analysis protocol ...
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bioRxiv - Neuroscience 2022Quote: ... slides were incubated for 5 minutes with DAPI (4’, 6-diamidino-2-phenylindole, 0.5ug/mL in 1X PBS) and were mounted using fluorescence mounting medium (Dako). Negative controls were included in each batch by omitting the primary antibody.
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bioRxiv - Pathology 2022Quote: ... DNA microarray analysis was performed using a Quick Amp labeling kit and a Whole Human Genome DNA Microarray 4×44K according to the manufacturer’s protocol (Agilent). Signal intensity was normalized by adjusting the data to a 75th percentile value ...
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bioRxiv - Neuroscience 2021Quote: iNeurons were replated on DIV 4 onto polyornithine/laminin coated in the Seahorse XF96 Cell Culture Microplate (Agilent Technologies) at a density of 50 000/well ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The spinal cord tissue was dissected and packed into a 4 mm zirconium rotor (Agilent Techonology Inc., CA, USA) with 50 μL of D2O saline (0.9% ...
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bioRxiv - Microbiology 2021Quote: ... and Tyr 254 in TlpALBD were generated via site-directed mutagenesis of pBH4_TlpALBD with primers listed in Supplemental Table 4 (QuikChange, Stratagene). TlpALBD and all point mutants were purified as described by Sweeney et al ...
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bioRxiv - Physiology 2024Quote: ... Sections were fixed with 0.2% glutaraldehyde + 4% PFA and mounted with Glycergel Mounting Medium (Agilent Technologies, Santa Clara, CA). The antisense probe generated with T7 polymerase showed the specific signal ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated overnight at 4°C with primary antibodies in DAKO REALTM Anti-body diluent (Agilent, Cat#S2022). Secondary antibodies (1:500 dilution ...
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bioRxiv - Microbiology 2024Quote: ... samples were cleared for 7 min at 14,000 × g at 4 °C and transferred to LC-MS polypropylene conical vials (Agilent). Vials were stored at −80 °C until analysis using LC-MS.
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bioRxiv - Genetics 2024Quote: ... Cryosections were incubated overnight at 4°C with primary antibodies: polyclonal anti-GFAP (Dako, Agilent, Santa Clara, California, USA) (1:5000 in 1% BSA ...
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bioRxiv - Genetics 2024Quote: ... Cryosections were incubated overnight at 4°C with primary antibodies: polyclonal anti-GFAP (Dako, Agilent, Santa Clara, California, USA) (1:5000 in 1% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... 4 technical replicates for each time point were analysed using Microwave Plasma - Atomic Emission Spectrometry (MP-AES 4210, Agilent) as reported previously24.
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bioRxiv - Neuroscience 2023Quote: ... and C21 was accomplished using a C18 Security guard cartridge (4.6 × 4 mm) and Zorbax Extend-C18 column (4.6 × 75 mm, 3.5 μm, Agilent 766953-902) at 40 °C (mobile phase A = H2O + 0.1% formic acid ...
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bioRxiv - Immunology 2024Quote: ... Sections were incubated overnight at 4°C with 10 µg/ml polyclonal rabbit anti-human/mouse fibrin(ogen) (Dako), 5 µg/ml rat anti-mouse C3b/iC3b (clone 3/26 ...
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bioRxiv - Zoology 2024Quote: ... a 50 µL aliquot of virus on parafilm (kept on ice) was exposed to 4 consecutive doses of 120,000 mJ of ultraviolet light using a Stratalinker UV Crosslinker (Stratagene) in a manner similar to previously published protocols (Mathew et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... GFP fluorescence was measured every 15 min up to 4 h and every 30 min thereafter (Mx3005P, Agilent Technologies). The slope of GFP fluorescence from 0 to 4 h was evaluated as the activity ...
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bioRxiv - Immunology 2024Quote: ... and gene expression microarray analysis was performed using the Agilent Whole Mouse Genome Microarray 4×44K v2 (Agilent Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibody solution was then washed out 3x with TBS-T and TBS-T + 5% NFDM + 1:2000 Dako goat anti-rabbit or anti-mouse HRP-conjugated immunoglobulins (Agilent, Santa Clara, CA, USA) was added ...
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bioRxiv - Cancer Biology 2020Quote: DNA from cell lines was isolated as previously described 12 and used to analyze copy number variations by the Array-CGH SurePrint G3 Human CGH Microarray 1×1M (Agilent, Santa Clara, California, USA). Array experiments were performed as recommended by the manufacturer ...
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bioRxiv - Biochemistry 2020Quote: ... The TIMS elution voltage was calibrated linearly to obtain reduced ion mobility coefficients (1/K0) using three selected ions (m/z 622, 922, and 1222) of the ESI-L Tuning Mix (Agilent, Santa Clara, CA, USA).
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bioRxiv - Biochemistry 2020Quote: ... Tyrosine fluorescence measurements were carried out in triplicate at 20 °C using a quartz cuvette (Hellma Asia Pte. Ltd. Singapore) at 1 cm path length on a Varian Cary Eclipse (Agilent Technologies Singapore Pte. Ltd) fluorescence spectrophotometer with excitation and emission wavelengths of 275 nm and 305 nm respectively ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 45 minutes at room temperature with the anti-SARS spike glycoprotein antibody 3A2 (rabbit, Abcam ab272420 1:100 diluted in Dako REAL antibody diluent #S2022), which has been validated in previous publications20,41 ...
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bioRxiv - Developmental Biology 2022Quote: ... Mutations were generated within the miR-1 seed sequences using the QuikChange Lightning or QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, California) to disrupt miR-1’s binding and regulatory function (Gregory et al. ...
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bioRxiv - Cell Biology 2022Quote: ... T cell culture medium was changed 1 hour before the start of readout into 180μL serum-free assay medium (Agilent Seahorse XF DMEM; 103575-100) supplemented with 4mM L-glutamine ...
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bioRxiv - Immunology 2022Quote: ... Phe322 was substituted for the Tyr322 residue of WT and G307S DNAM-1 by using QuikChange II Site-Directed Mutagenesis Kits (Agilent technologies, Palo Alto, CA) with primers (5′-GAGAGGATATTTTTGTCAACTATCCAACC-3′ and 5′-GGTTGGATAGTTGACAAAAATATCCTCTC-3′) ...
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bioRxiv - Pathology 2020Quote: ... Abcam, Cambridge, MA), CD68 (LV n=25, IVS n= 26, RV n=22) for macrophages (1:800, clone KP1, Dako/Agilent, Santa Clara, CA), and CD163 (LV n=26 ...