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Citations for Agilent :
2651 - 2700 of 3923 citations for 1 1' 3' 1'' Terphenyl 4 4'' dimethanamine 5' 4 aminomethyl phenyl 9CI since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Serum samples were diluted 1:50 in PBS + 25% FBS and data were acquired with a BioTek Synergy H1 plate reader (Agilent). ELISA data were analyzed with GraphPad Prism (Dotmatics ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then washed and re-suspended in HBSS with 2% FBS with 1 μg/ml propidium iodide before acquisition on a Novocyte Quanteon or Penteon (Agilent).
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bioRxiv - Immunology 2024Quote: ... The single and triple-site mutations were introduced into the pcDNA3.1 vector encoding SARS-CoV-2 wildtype or BA.1 Spike using QuickChange site-directed mutagenesis (Agilent 210519) according to the instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Frozen 10 µm sections were prepared using a cryostat and immunostained with guinea pig anti-insulin polyclonal antibody (1:100; Dako IR002, Agilent Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Frozen 10 µm sections were prepared using a cryostat and immunostained with guinea pig anti-insulin polyclonal antibody (1:100; Dako IR002 ...
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bioRxiv - Cancer Biology 2024Quote: ... The LZK Q240S drug-resistant construct and K195M kinase-dead mutant (1) was introduced using a Site-Directed Mutagenesis Kit (Stratagene) (Primers listed in Supplementary table S2) ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescence was monitored with excitation and emission filters set at 465 and 590 nm during gradual heating (1 K/min) from 25 °C to 95 °C in a MX3005P real-time PCR instrument (Stratagene). Data was evaluated using MXPro3005 software (Stratagene ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were washed 5 times with 1X PBST and incubated with horseradish peroxidase conjugated secondary anti-mouse or anti-rabbit IgG (1:5000, Cat # P0161 and P0448, respectively; DAKO) in 1X PBST for 1 h at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were washed three times with TBS/T before HRP-coupled secondary antibodies were applied 1:25000 in TBS and incubated for 2 h at RT (Polyclonal rabbit anti-mouse, Dako P0260 ...
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bioRxiv - Biochemistry 2024Quote: ... the peptide arrays were incubated with a 1:1,000 dilution of an appropriate antibody peroxidase conjugate (anti-rabbit immunoglobulins HRP conjugate, DAKO, P0217) for one hour at 25°C ...
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bioRxiv - Cell Biology 2024Quote: ... A 96-well flat bottom MaxiSorp immuno plate was coated with 1 µg/mL anti-vWF antibody (Dako rabbit polyclonal) in a sodium carbonate/bicarbonate buffer pH 9.5 for 1 h at room temperature prior to blocking with 3% w/v BSA in PBS-T for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... RPE1 cells (1×104) of the indicated genotypes were seeded in 96-well Seahorse XFe96 cell culture microplates (Agilent Technologies) for 24 h in eight replicate wells ...
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bioRxiv - Molecular Biology 2022Quote: 3*10^3 of HPLFs per well were seeded into Seahorse XFe96 well plate (Agilent Technologies, 200941) for overnight ...
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bioRxiv - Biophysics 2021Quote: ... The thermal variations were in the range 25-95°C in a stepwise increment of 1 °C/minute step and using a 96-well PCR plate (Agilent Technologies) sealed with optical quality sealing tape (Microseal® ‘B’ seal from BIO-RAD) ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to synthesize cDNA from 1 ug RNA and Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies) was used for the Quantitative RT-PCR reactions ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by 1 hour incubation with secondary antibodies diluted in 2.5% milk in TBST (1:5000, goat anti-rabbit HRP, or goat anti-mouse HRP, DAKO, #P0448 & #P0447). Membranes were washed 3 times with TBST and exposed to HRP substrate (Immobilon Crescendo ...
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bioRxiv - Microbiology 2020Quote: The quality of purified libraries was analyzed on a Bioanalyzer High Sensitivity DNA chip (Agilent; see Figure 1 for an example) by following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... The following secondary antibodies were added for a 1-hour incubation at room temperature: anti-mouse IgG HRP-linked (Dako, p0447) and anti-rabbit IgG HRP-linked (Cell signaling ...
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bioRxiv - Cell Biology 2020Quote: 3000 control or p107KO cells or p107KO cells 24 hours post transfection with pCMV6-OCT-HA-eGFP alone or together with p107fl or p107mls were seeded in DMEM (Wisent) containing 10% FBS and 1% penicillin streptomycin on microplates (Agilent Technologies) and treated according to the required experiment ...
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bioRxiv - Immunology 2021Quote: Total IgG from individual donors was purified from ∼1 µL plasma using the AssayMAP Bravo platform (Agilent Technologies, Santa Clara, USA) with Protein G-coupled cartridges as described elsewhere (Larsen et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were treated with AEVs from AD and normal participants for 1 hour and then subjected to fluorescent immunolabelling for MAC using a mouse anti-human C5b-9 antibody (1 μg/mL; RRID: AB_2067162, cat. no. M0777, Dako, Carpinteria, CA) targeting an epitope in the poly-C9 component of MAC in association with C5b ...
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bioRxiv - Developmental Biology 2022Quote: ... we washed the cells twice with 1X PBS and incubated them with FITC-conjugated anti-mouse IgG (dilution 1:500; Dako Cytomation) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... The quality of the libraries is determined using the Standard High sensitivity NGS Fragment analysis kit (DNF-474, 1-6000 base pair) on the Agilent Fragment analyzer (Agilent, USA), yielding approximately 260 bp size fragments ...
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bioRxiv - Plant Biology 2020Quote: ... RT-PCR was performed with 1μL of diluted cDNA (1:9) in a Brillant III ultra fast SYBRgreen QPCR master mix (Agilent Technologies, USA) using a StepOnePlus Real-Time PCR System (Applied biosystems ...
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bioRxiv - Bioengineering 2020Quote: ... the sections were incubated with the secondary antibody (goat anti mouse IgG HRP, DAKO P0447, 1:100, EnVision+; System-HRP anti-rabbit DAKO, K4003) for 60 min at RT and stained using DAB peroxidase substrate solution (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... and were then incubated in a solution containing mouse antibody anti-Aβ (clone 6F/3D; 1:50, Dako M0872, Glostrup, Denmark). The sections were then processed with a secondary biotinylated horse anti-mouse IgG antibody (1:200 ...
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bioRxiv - Biophysics 2020Quote: ... Human SHP-1 with an N-terminal 6x His tag was produced in Escherichia coli BL21-CodonPlus (DE3)-RIPL strain (Agilent Technologies) and purified on Ni2+-NTA agarose (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... and then diluted to 1 ng/uL before checking insert size on an Agilent Technologies 2100 Bioanalyzer (Agilent Technologies; Waldbronn, Germany) and quantifying to greater accuracy by quantitative Q-PCR (library molarity >2 nM) ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... The extent of shearing was monitored with a 1% agarose gel and confirmed by separation on a 2100 High sensitivity Bioanalyzer chip (Agilent Technologies) upon completion of the immunoprecipitation ...
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bioRxiv - Biochemistry 2020Quote: ... The combined organic extract was concentrated to ~1 mL prior to clean-up by SPE (Agilent Bond Elut SI, 500 mg). The SPE columns were conditioned with hexane and the extract eluted with 6 mL 10% dichloromethane in hexane ...
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bioRxiv - Neuroscience 2020Quote: ... and then diluted to 1 ng/µl before checking insert size on an Agilent Technologies 2100 Bioanalyzer (Agilent Technologies; Waldbronn, Germany) and quantifying to greater accuracy by quantitative Q-PCR (library molarity >2 nM) ...
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bioRxiv - Microbiology 2020Quote: ... The secondary antibodies used were as follows: HRP-conjugated goat anti-mouse (1:5,000 dilution, Dako Agilent, Santa Clara, CA, USA), HRP-conjugated rabbit anti-hamster (1:2000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... The secondary antibodies used were as follows: HRP-conjugated goat anti-mouse (1:5,000 dilution, Dako Agilent, Santa Clara, CA, USA), HRP-conjugated rabbit anti-hamster (1:2000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... we created pcDNA3-AhR-1(LBD)-VP16 by site-directed mutagenesis of the pcDNA3-AhR-1-VP16 plasmid with the QuikChange II Site-Directed Mutagenesis Kit (Agilent, 200523). The following primer pair was used to create an L to A substitution at L363 ...
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bioRxiv - Cell Biology 2022Quote: ... Arrays were then washed with probing buffer (0.1% Tween 20, 1% BSA, 10% PBS) and incubated for 1.5 h in a hybridization chamber (Agilent, Santa Clara, CA) in a reaction mixture containing 80 μg of purified His-SETD6 in probing buffer in a total reaction volume of 950 μl ...
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bioRxiv - Neuroscience 2021Quote: ... and the membrane was blocked with 5% milk in tris-buffered saline with Tween 20 (TBST) before immunodetection with the following primary antibodies: tau (1:1000, DAKO, Denmark), pTau-S396 (1:500 ...
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bioRxiv - Plant Biology 2021Quote: ... samples by using modified CTAB and LiCl method [24,25] The quality of isolated RNA measured by UV spectrophotometer at 260/280and 260/230 nm wavelengths and 1% agarose gel electrophoresis followed by measuring RNA concentration using a 2100 Bioanalyzer (Agilent Technologies). The concentration of RNA was obtained in SaSHc 1460.90 ng/μl and in SaSLc 12.65 ng/μl ...
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bioRxiv - Cancer Biology 2021Quote: ... W503F (PBD, polo-box domain 1) and H629A, K631M (PBD, polo-box domain 2) were generated by site-directed mutagenesis (Agilent Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The PmeI-linearized pShuttle-vectors carrying different cDNA fragments were introduced into the replication-deficient adenoviral vector pAdEasy-1 through homologous recombination in BJ5183 cells (Agilent Technologies). Successfully recombined adenoviral vector was verified by sequencing ...
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bioRxiv - Systems Biology 2020Quote: ... Following washes in phosphate buffered saline (PBS) the slides were stained with 1:500 anti-GFAP rabbit polyclonal antibody (DAKO, Z0334) diluted in 1% blocking reagent in Tris buffered saline (Roche Applied Science ...
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bioRxiv - Biochemistry 2020Quote: ... Extracts of the cross-linked pellets were processed into sequencing libraries as previously described (Bohnsack, Tollervey, and Granneman 2012) using 1µl of a 1:100 dilution of 10U/µl RNace-IT (Agilent Technologies, 400720) per sample ...
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bioRxiv - Genetics 2021Quote: ... and the quality of the sample was checked by running a 1% agarose gel and by RNA 6000 Nano assay on a 2100 Bioanalyzer (Agilent Technologies).
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bioRxiv - Neuroscience 2020Quote: ... Membranes were subsequently washed and incubated with horseradish peroxidase-conjugated secondary antibody (goat anti-rabbit IgG, 1:20,000, Dako, Carpenteria, CA). All primary and secondary antibodies were diluted in TBST containing 5% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were treated with 1:10 normal goat serum in PBS for 15 minutes and incubated for 60 minutes with 1:200 diluted Rabbit anti-human thyroglobulin (Dako, Denmark) or 1:400 diluted Rabbit anti-human calcitonin (Dako ...
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bioRxiv - Cancer Biology 2021Quote: WT and CCN4-KO melanoma cells were cultured overnight (1 × 104 cells/well) in Seahorse XFe96 cell culture microplates (Agilent, CA) with complete DMEM medium ...
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bioRxiv - Biochemistry 2021Quote: ... Secondary antibodies were horseradish peroxidase-conjugated goat anti-mouse or goat anti-rabbit (used at dilution of 1:2,000; Dako UK, Ltd.). Reactive proteins were visualized by chemiluminescence with ECL plus (Amersham plc).
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bioRxiv - Immunology 2021Quote: ... 1) were elucidated by 1D and 2D NMR [Varian MERCURY plus AS400 (400 MHz)] and HRTOF-MS spectroscopy (Agilent 1200/6530) (Supplemental Table 1).
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bioRxiv - Synthetic Biology 2021Quote: ... Desorption was performed at 250 °C for 1 minute (splitless mode) in the injection port of a 6890N gas chromatograph (Agilent Technologies). After desorption ...
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bioRxiv - Immunology 2021Quote: ... Exome sequencing was performed on genomic DNA from Patients 1 and 2 and their parents using a SureSelect Human All Exon kit (Agilent Technologies) for targeted enrichment ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies were directed against: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), epithelial membrane antigen (EMA ...