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Citations for Agilent :
2601 - 2650 of 3831 citations for Platelet endothelial aggregation receptor 1 PEAR1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Unbound antibody was removed with washing buffer and the fraction of cells with surface protein labeled with CD44 antibody was determined using a MoFlo cell sorter (Dako Cytomation).
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with antibody polyclonal Guinea Pig anti-Human Insulin (cat. A21435 - Agilent Technologies, Santa Clara, CA, USA) diluted 1:1740 in BSA 1% in PBS without Ca2+ and Mg2+and with monoclonal mouse anti-Human ACE2 (cat ...
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bioRxiv - Immunology 2022Quote: ... tissues fixed for 24-30 hours in 10% neutral-buffered formalin (NBF; 3.7% formaldehyde) as previously described (Wiarda et al., 2020) with polyclonal rabbit α-human CD3ε antibody (Dako A0452) and polyclonal goat α-rabbit horseradish peroxidase (HRP)-conjugated antibody (Dako K4003) ...
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bioRxiv - Molecular Biology 2021Quote: ... dilution 1:100)] in TNB blocking buffer for overnight at 4°C followed by one-hour incubation with secondary antibodies: EnVisionTM+ System-HRP (DAKO K4002). TRITC-based Tyramide reagent pack from Perkin Elmer was used to amplify the fluorescence of Ki67 and c-Kit ...
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bioRxiv - Microbiology 2020Quote: ... for 2hrs at 40°C and then incubated over-night at 4°C on a rotating wheel with 2 μg of anti HBc antibody (Dako B0586). Immune-complexes were captured with protein A/G magnetic beads ...
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bioRxiv - Cell Biology 2021Quote: Paraffin-embedded islet grafts within the kidneys or intact mouse or human islets were stained for insulin using polyclonal guinea pig anti-insulin antibody (Dako, A0564), and apoptosis was measured using the DeadEnd Fluorometric terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL ...
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bioRxiv - Microbiology 2020Quote: ... ACE2 was detected using a rabbit polyclonal anti-hACE2 antibody (Cell Signalling, 4355S; citrate antigen-retrieval) and EnVision+ anti-rabbit HRP (Agilent, K4003). Slides were scanned with a bright field slide scanner (Leica ...
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bioRxiv - Cancer Biology 2020Quote: ... paraffin-embedded human sections were immunolabeled for CD68 and CD163 (details of antibodies are available in Supplementary Table S3) and counterstaining with Mayer’s hematoxylin (cat. #S3309; Dako, Jena, Germany). Immunohistochemical images were analyzed in collaboration with the anatomical pathology laboratory of Hospital North using Calopix software (TRIBVN ...
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bioRxiv - Neuroscience 2022Quote: ... 3x 5 minutes) and incubated in the secondary antibody-horseradish peroxidase (HRP) complex as part of REAL EnVision detection system (Dako #K5007) for 1h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... For secondary antibody staining, either ImmPRESS kits (anti-goat, MP-7405; anti-rat, MP-7444) or Envision+ Reagents (Dako: rabbit, K4002) were used ...
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bioRxiv - Genetics 2020Quote: ... serial sections were stained singly with antibodies against BCL6 and CD8 and a DAB DAB visualization kit (Envision Double Stain system, Dako; USA) for bright field microscopy ...
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bioRxiv - Pathology 2020Quote: ... HER2/neu and Ki-67 was accomplished on FFPE tissue segments as part of the routine clinical assessment of these cases using anti-ER antibody (DAKO, Denmark),anti-ER antibody (DAKO ...
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bioRxiv - Physiology 2021Quote: ... sections were immunostained with alpha-smooth muscle actin (α-SMA) antibody (Clone 1A4, DAKO, M0851, Agilent Technologies, Santa Clara, CA, USA). Slides containing gastrocnemius muscle sections were deparaffinized in xylene and rehydrated using a graded ethanol series ...
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bioRxiv - Neuroscience 2021Quote: The optimal concentration was determined for each antibody: CD3 (polyclonal rabbit – anti-human CD3 IgG, cat. no. A045201-2, Agilent Technologies), 1:60 ...
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bioRxiv - Molecular Biology 2020Quote: ... then incubated with horseradish peroxidase-conjugated rabbit anti-goat or swine anti-rabbit or rabbit anti-mouse antibodies (Dako, Agilent Technologies) and incubated at room temperature for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... The secondary antibodies were horseradish peroxidase- conjugated goat anti-rabbit IgG antibodies (Nichirei, 424141) for HRP-IHC and visualized using a Liquid DAB+ Substrate Chromogen System (DAKO, K3468), or Alexa Fluor Plus 488- or 555-conjugated goat anti-mouse ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... the blots were incubated with secondary antibodies: horseradish peroxidase-conjugated goat anti-mouse (#P0047; Dako Products Agilent, Santa Clara, CA, USA) or goat anti-rabbit (#A00160 ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... the blots were incubated with secondary antibodies: horseradish peroxidase-conjugated goat anti-mouse (#P0047; Dako Products Agilent, Santa Clara, CA, USA) or goat anti-rabbit (#A00160 ...
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Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosisbioRxiv - Neuroscience 2024Quote: ... the sections were incubated with a secondary antibody labeled with a polymer as part of the Envision+ system containing horseradish peroxidase (Dako Cytomation). The following primary antibodies and dilutions were used to stain mouse tissues ...
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bioRxiv - Cancer Biology 2024Quote: ... The sections were rinsed with wash buffer and appropriate secondary antibody was applied for 30 min (Cas9, Mouse Envision (Agilent, K4001); Ku80 ...
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bioRxiv - Cancer Biology 2024Quote: Sections from primary tumors and the matched axillary node with the largest metastatic focus (≥ 2 mm) were used for assessment of nerves (Neurofilament antibody, DAKO M0762), and angiogenesis markers (Factor-VIII ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed 3 times for 10 minutes each with TBST and probed with appropriate HRP secondary antibody (anti-Rabbit Immunoglobulin/HRP, Dako P044801 or anti-mouse Immunoglobulin/HRP, Dako P0260) at 1:1000 in TBST with 5% skim milk ...
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bioRxiv - Neuroscience 2022Quote: ... total tau was enriched by IP with the polyclonal K9JA anti-tau antibody clone (Dako, cat. no. A0024, Lot No. 20043728) from 400 μg protein lysate for the Exon 1 MAPT+/+ and MAPT-/- #2 lines ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatants from 1mL transient transfections of the antibodies were purified using the AssayMAP BRAVO platform with 5mL Protein A cartridges (Agilent Technologies). Lager scale transient transfections were purified on the Akta FPLC system using 1mL HiTrap MabSelect PrismA™ affinity chromatography resin (Cytiva) ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated with anti-rabbit or anti-mouse horseradish peroxidase- labelled secondary antibody (Dako EnVision+ System- HRP Labelled Polymer) followed by a chemiluminescent reaction (Clarity Western ECL Substrate ...
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bioRxiv - Bioengineering 2023Quote: ... Specific site directed mutations on the S309 and CMAB0 antibody sequence was done using the Quick-change site directed mutagenesis kit II (Agilent technologies).
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bioRxiv - Cell Biology 2023Quote: ... Samples from the primary human monolayer experiment were fixed and immunostained for insulin with guinea pig anti-insulin primary antibody (Dako A0564) and Alexa-Fluor 568 goat anti-guinea pig secondary antibody (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were washed in TBS-t and subsequently incubated with goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody (0.5 µg/ml; Dako, VIC, Australia) or rabbit anti-Goat IgG horseradish peroxidase-conjugated secondary antibody (0.125 µg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... The reporter cell lines were stained with anti-human CD45 and anti-human CD19 antibodies and analyzed by flow cytometry on an ACEA NovoCyte 2060 (Agilent, USA). ACEA NovoExpress (Agilent ...
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bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...
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bioRxiv - Neuroscience 2024Quote: ... The brain sections were washed trice in wash buffer (1:50 dilution of the incubation buffer in PBS) followed by incubation for 60 min with biotinylated rabbit-anti-mouse antibody (Dako, E0354) or biotinylated goat ant-rabbit antibody (Vector ...
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bioRxiv - Cancer Biology 2024Quote: ... the sections were incubated for 15 min with an anti-mouse secondary antibody conjugated to a dextran-labeled polymer and HRP (DakoCytomation K4007), followed by incubation in DAB solution (DakoCytomation DAB # K4007 ...
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bioRxiv - Cell Biology 2024Quote: ... The antibody-vWF complexes were washed with PBS-T (0.1%) three times followed by incubation with an anti-vWF antibody (Dako rabbit polyclonal) conjugated with biotin for 60 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... was applied for 30 min at a concentration of 0.108 µg/mL at room temperature using Antibody Diluent (S302283-2, Agilent Technologies Inc). Detection was performed using the Bond Polymer Refine Kit (DS9800 ...
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bioRxiv - Cell Biology 2024Quote: ... they were incubated with fluorophore-conjugated antibodies targeting specific cell surface markers and then analyzed using a NovoCyte flow cytometer (Agilent, USA). The antibodies used for this analysis are as follows ...
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bioRxiv - Cell Biology 2020Quote: ... followed by overnight incubation at 4 °C with α-α-SMA (1:400, mouse monoclonal, clone 1 A4, DAKO Agilent Technologies, Santa Clara, USA) or α-CD68 (1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by overnight incubation at 4 °C with α-α-SMA (1:400, mouse monoclonal, clone 1 A4, DAKO Agilent Technologies, Santa Clara, USA) or α-CD68 (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed cells were further incubated overnight in 1% NHS in TBSTx with rabbit anti-GFAP (diluted 1:500; Dako-Agilent, Santa Clara, CA, USA), rabbit anti-S100B (1:400 ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed cells were further incubated overnight in 1% NHS in TBSTx with rabbit anti-GFAP (diluted 1:500; Dako-Agilent, Santa Clara, CA, USA), rabbit anti-S100B (1:400 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The hybridisation mix was prepared by mixing the specified amounts of RNA with 10× Gene Expression Blocking Agent (1× final conc.) and 2× Hi-RPM Hybridization Buffer (1× final conc.) (Agilent, Cat No. 5188-5242) in a final volume of 42 μl ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Subunits were diluted 1:500 or 1:1000 in nuclease-free ultrapure water and OD 260nm was measured (Agilent Cary 60 UV/VIS spectrophotometer). Subunit concentrations were calculated as described previously such that 1 A260 unit corresponds to 25 pmol of 60S and 50 pmol of 40S respectively64 ...
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bioRxiv - Genomics 2022Quote: ... 1 μL was quantified with a Qubit Fluorometer while another 1 μL aliquot was run on the BioAnalyzer 2100 (Agilent Technologies, Santa Clara, CA) to check the library quality and determine the weighted average fragment size ...
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bioRxiv - Neuroscience 2022Quote: ... and CD68 (Monoclonal Mouse Anti-Human, EDTA Buffer HIER, IHC dilution 1:5000, IF dilution 1:500, Clone L26, Dako Omnis, Code Number: M0756) were employed to characterize lympho-monocytic infiltrations ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 µg of tumor DNA extracted from fresh-frozen tissue and 1 µg of male human reference DNA control (Agilent Technologies, Santa Clara, CA) were digested with AluI and RsaI ...
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bioRxiv - Neuroscience 2024Quote: ... phosphorylated tau (PHF1; 1:1000, kind gift of P. Davies (Pensalfini et al., 2020) and total tau protein (Agilent Cat# A0024, RRID:AB_10013724; 1:5000). All the secondary antibodies for western blot analyses were used according to the manufacturer’s recommendations (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... coli XL−1 Blue cells (Agilent Technologies, Santa Clara, CA) were transformed with assembled plasmids according to the provider’s instructions and stored at −80 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-PCNA (1:500, Dako, Agilent, Santa Clara, California), rabbit anti-S100β (1:400 ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-PCNA (1:500, Dako, Agilent, Santa Clara, CA) and rabbit anti-S100 (1:400 ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-PCNA (1:500, Dako, Agilent, Santa Clara, CA) and rabbit anti-S100 (1:400 ...
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bioRxiv - Developmental Biology 2020Quote: ... Guinea Pig anti-Insulin pre-diluted 1:6 (Dako 1R002), Chicken anti-GFP 1:1000 (Abcam ab13970) ...