Labshake search
Citations for Agilent :
2601 - 2650 of 3949 citations for Mouse Anti Hepatitis C Virus NS5a Antibody 1877 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Antigen concentration was determined using a PLRP-S column (2.1 x 150 mm, 300Å, 3μm) operated at 0.5 mL/min and 60°C (Agilent Technologies, Santa Clara, CA). Buffer A was 0.1% (v/v ...
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bioRxiv - Molecular Biology 2019Quote: ... Each Hi-C library (750 ng) was hybridized and captured individually using the SureSelectXT Target Enrichment System reagents and protocol (Agilent Technologies). After library enrichment ...
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bioRxiv - Cell Biology 2021Quote: ... Splitless injection (injection temperature 270 °C) onto a 30 m + 10 m × 0.25 mm DB-5MS + DG column (Agilent J&W) was used ...
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bioRxiv - Synthetic Biology 2021Quote: ... Desorption was performed at 250 °C for 1 minute (splitless mode) in the injection port of a 6890N gas chromatograph (Agilent Technologies). After desorption ...
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bioRxiv - Developmental Biology 2021Quote: ... for 6 minutes (30 seconds ON, 30 seconds OFF) at 4°C to obtain ~200 bp fragments (confirmed using an Agilent Bioanalyzer). Sonicated DNA was extracted using ethanol precipitation ...
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bioRxiv - Systems Biology 2022Quote: ... An Agilent 1200 Series HPLC system was used with a refraction index detector at 50°C (Agilent, Santa Clara, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice and incubated for 1 h at 37°C in the non-CO2 chamber with 180 μl of the assay medium (Agilent; 103575) supplemented with L-glutamine (2 mM ...
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bioRxiv - Cell Biology 2022Quote: ... treated cells were then equilibrated in seahorse XF base medium supplemented with 1g/L glucose at 37°C in non-CO2 incubator for 1 hour prior to analysis using a Seahorse XF24 Extracellular Flux Analyzer (Seahorse Bioscience).
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bioRxiv - Physiology 2022Quote: ... sections were dewaxed and epitope retrieval was performed using Citrate buffer pH6 for α-SMA and Ter119 or Tris- EDTA buffer pH9 for HMOX1 in all cases for 20 min at 97°C using a PT Link (Dako, Agilent) or with ER2 buffer (AR9640 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Hydrolyzed amino acids were separated using ultra performance liquid chromatography (UPLC, Acquity - Waters, USA) on a C-8 column (Zorbax Eclipse XBD - Agilent, USA) at a flow rate of 0.6 mL/min and eluted off the column using a hydrophobicity gradient ...
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bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
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bioRxiv - Biophysics 2024Quote: In vitro NMR experiments were carried out at 25 °C on an 800 MHz Agilent spectrometer equipped with a cryogenic probe (Agilent Technologies). The buffer used in all NMR titration assay was 50 mM sodium phosphate buffer (pH 6.5 ...
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bioRxiv - Physiology 2024Quote: Fuel preference of CRISPR/Cas9 Pparγ edited cells (γY4KO) and mock-edited cells (C) was measured using Seahorse Mito-Fuel Flex test (Agilent Technologies), in assay medium as above and according to the manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: ... Epitope retrieval was then performed at 95 °C for 10 min at pH 9 with Dako Target Retrieval Solution (Agilent, USA), in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... The plate was sealed with a gas permeable seal (Azenta Life Sciences 4ti-0516/96*) and incubated at 37°C and 237 cpm for indicated times in a Synergy H1 plate reader (Agilent BioTek). OD600nm was measured every 10 min ...
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bioRxiv - Immunology 2022Quote: ... the tissue sections for IMC were heat pre-treated using a target retrieval solution for 30 min at 95°C (K8000, EnVision flex target retrieval solution, pH9 (Agilent Technologies). Slides were cooled down for 20 min at room temperature before rinsing with water and PBS ...
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bioRxiv - Immunology 2022Quote: ... for 120 s and separated by a C-18 column (Zorbax 300SB 2.1 × 50 mm, 3.5 μm particle diameter, Agilent, Santa Clara, CA). For LC ...
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bioRxiv - Cancer Biology 2023Quote: ... Heat-induced epitope retrieval (HIER) was performed by immersing the tissue sections at 98°C for 20 minutes in LowFlex (Dako #K8005). Staining was performed following the epitope retrieval process using VectaStain Kit from Vector Labs for cleaved Caspase-3 and horseradish peroxidase-labeled polymer from Dako (K4001 ...
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bioRxiv - Biophysics 2023Quote: All absorption and emission measurements for both labeled A2AAR and Rec were carried out at 25 °C on a Synergy H4 (Agilent BioTek) plate reader with Take3 Microvolume Plates ...
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bioRxiv - Bioengineering 2023Quote: ... The electrode temperature was controlled by submerging in 70 °C PBS solution with a P4010 thermal meter (Dostmann electronic, Germany) for temperature monitoring and a 34401A digit multimeter (Agilent, USA) for electrical resistance measurement ...
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bioRxiv - Cancer Biology 2023Quote: ... GC-MS analysis was performed using an Agilent 7890 A GC coupled to an Agilent 5975 C inert XL Mass Selective Detector (Agilent Technologies). A sample volume of 1 μL was injected into a Split/Splitless inlet ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor xenograft slides were incubated at 60 °C for 2 h followed by antigen retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
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bioRxiv - Molecular Biology 2023Quote: ... desorption was performed at 250°C for 1’ (splitless mode) in the injection port of a 6890 N gas chromatograph (Agilent Technologies) coupled to a 5975B mass spectrometer (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated at 37°C in a non-CO2 incubator for 1 hour and subsequently loaded into the Seahorse XFe96 Analyzer (Agilent Technologies, Seahorse Bioscience) when prompted ...
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bioRxiv - Genomics 2023Quote: The fragment size distribution and concentration of the final PacBio and Dovetail Hi-C libraries were assessed using the TapeStation (Agilent Technologies) and the Qubit Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... at 40°C with an InfinityLab Poroshell 2.7 µM EC-C18 pre- column (3 x 5 mm; Agilent Technologies, Waldbronn, Germany) was used ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen retrieval was performed in a Tris-EDTA buffer for 30 min at 95°C for Ki-67 (1:200, Dako, M7240), ATRX (1:400 ...
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bioRxiv - Immunology 2023Quote: ... Antigen retrieval was then performed for 20 minutes at 95°C using Lecia Epitope Retrieval Buffer 2 followed by treatment with Dako serum-free protein block (X090930-2, Agilent Dako) for 15 minutes to prevent non-specific binding of the antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... were constructed from pcDNA3.1+-C-(K)-DYK-EBP plasmids using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, #210519) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... One hundred micrograms of precipitated protein sample were digested with trypsin and 30 µg of the resulting peptides desalted with PepClean C-18 Spin Columns (Agilent Technologies) according to manufacturer recommendations as previously described (Martinez-Marquez et al ...
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bioRxiv - Neuroscience 2023Quote: Vibratome cut (free floating) tissue was heated to 70°C in 1x Daco Target Retrieval solution (DakoTarget Retrieval Solution, pH 6.1, Dako, code. S2367) for 15 min and left to cool at room temperature (RT) ...
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bioRxiv - Microbiology 2023Quote: ... The Seahorse XFp extracellular flux cartridge was hydrated with sterile water and incubated overnight at 30 °C and Seahorse XF calibrant solution (Agilent Technologies). After this ...
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bioRxiv - Biophysics 2023Quote: ... Cuvettes were then inserted into a preheated (50°C) Cary 3500 UV−Vis spectrophotometer controlled by an Agilent multizone Peltier temperature controller (Agilent Technologies). The samples were cooled to 1°C at a rate of 1°C/min while measuring the absorbance at 600 nm.
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bioRxiv - Cell Biology 2023Quote: ... and the N-terminal 6xHis-tagged and C-terminal EGFP-tagged proteins were expressed in Arctic Express (DE3) RP cells (Agilent Technologies). Bacterial cultures in LB medium were induced with 0.1-0.2 mM IPTG at exponential growth phase (OD600 = 0.5-0.6 ...
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bioRxiv - Microbiology 2024Quote: ... Cultures were grown at 30 °C in 96-well plates in an BioTek Epoch 2 shaking incubator (Agilent, Santa Clara, CA) for 72 hours.
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bioRxiv - Evolutionary Biology 2024Quote: ... was fragmented and hybridized to a microarray for 17 h while rotating at 10 rpm at 65 °C in a hybridization oven (Agilent Technologies). The hybridized microarray was washed and scanned according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: The purified RNAs in the 96-well PCR plate (Axygen Scientific, PCR-96-FS-C) were transferred to a 0.3 mL 96-well plate (Agilent, 5043-9313) for hydrolysis in a 40 µL of an enzyme cocktail containing 10 U benzonase (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... sections were deparaffinized, dehydrated and subjected to antigen retrieval (20 min incubation at 98 °C in citrate buffer, pH 6 (Agilent Dako) for CD8 ...
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bioRxiv - Microbiology 2024Quote: ... Log-phage cultures were inoculated into specified media and monitored for growth rate every 15 min at OD600 for 24 or 72 hours at 37°C under static conditions using BioTek Synergy H1 (Agilent Technologies).
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bioRxiv - Genomics 2021Quote: ... Floating sections were permeabilized, blocked, probed by Iba-1 antibody (Wako, catalog # 019-19741, and 1:800 dilution) or GFAP antibody (DAKO, catalog # z0334, and 1:500 dilution), inactivated endogenous peroxidases ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were incubated with HPR-conjugated secondary antibodies (Agilent, K400311-2) for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... MA172531) total Tau antibody K9JA 1:500 (Dako, cat no A0024), anti-mouse secondary antibody conjugated with Alexa flour-488 1:500 (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... placed into a wet chamber and blocked with antibody diluent (DAKO) for 1 hour at RT ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (Dako) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Dako, Agilent Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... the sections were incubated with Envision Flex HRP secondary antibody (Dako) for 30 minutes and color was developed using liquid DAB Substrate ...
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bioRxiv - Cancer Biology 2019Quote: ... and secondary antibody horseradish peroxidase (DAKO Agilent, Santa Clara, CA, USA). Endogenous peroxidase activity was blocked using 3% hydrogen peroxide ...
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bioRxiv - Cancer Biology 2019Quote: ... and secondary antibody horseradish peroxidase (DAKO Agilent, Santa Clara, CA, USA). Endogenous peroxidase activity was blocked using 3% hydrogen peroxide ...
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bioRxiv - Cancer Biology 2021Quote: ... underwent antigen retrieval and were incubated with primary antibodies (KIT, DakoCytomation/Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibodies (rb-GFAP 1:200, Dako, gt-Iba1 ...