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Citations for Agilent :
2501 - 2550 of 2722 citations for 7 Bromomethyl 1 benzothiophene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Ki-67 was scored on the glass slide of whole tissue sections by a breast pathologist (BC) according to the Ki-67 IHC MIB-1 pharmDx (Dako Omnis) Interpretation Manual for Breast Carcinoma ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were the following: goat anti-mouse IgG conjugated to horseradish peroxidase (IB: 1:10,000, cat#P044701-2 Dako, Glostrup, Denmark), donkey anti-Rabbit IgG Alexa Fluor 488 (IF ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... neurons were incubated for 1 hour in CO2-free incubator in Agilent Seahorse DMEM assay medium (Agilent Technologies, cat# 103575-100) supplemented with 1 mM sodium pyruvate (Agilent Technologies ...
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bioRxiv - Immunology 2024Quote: ... 11 cycles of TCR target enrichment PCR 1 and 2 were performed and the resulting cDNA was quantified on an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies). TCR libraries were prepared and indexed with 9 cycles of amplification using the PN-220103 Chromium i7 Sample Index Plate ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated with primary antibodies (chicken anti-Map2 [Thermo Scientific, PA1-16751, 1:1000] and rabbit anti-Gfap [Dako, Z0334 ...
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bioRxiv - Biochemistry 2023Quote: ... blots were incubated for 1h at room temperature with a horse radish peroxidase-labeled secondary antibody (anti-rabbit, Dako; 1/1000), and revealed by ECL chemiluminescence (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10 mM glucose (for the Cell Mito Stress Test Kit) or 1 mM glutamine (for the Glycolysis Stress Test Kit) was used as the assay medium (103681-100; Agilent Technologies). Briefly ...
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bioRxiv - Pathology 2023Quote: ... EGTA 1 mM and 1mg/ml bovine serum albumin) supplemented with respiratory substrates (glutamate/malate) and 50 nM MitoXpress (Agilent, USA). Each well was loaded with 10μl respiratory substrates ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with DAPI (1 μg/ml) and mounted onto glass slides using DAKO Fluorescent Mounting Medium (Agilent Technologies S3023). Images were acquired using an Upright ZEISS AXIO Imager.M2 with Apotome 2.
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bioRxiv - Plant Biology 2024Quote: ... Powdered samples (1 g) were weighed and transferred immediately to a 20 mL head-space vial (Agilent, Palo Alto, CA, USA), containing NaCl saturated solution to inhibit potential enzyme reactions ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated with Alexa Fluor®-conjugated secondary antibodies for 1 hour at room temperature and mounted on slides using DAPI-containing mounting media (Dako). Images were acquired using a Zeiss LSM 800 series confocal microscope at the Yale CCMI facility ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with primary antibody against pS65-Ub (in- house, 1:250) [20] that was diluted in Dako Antibody Diluent with Background Reducing Components (Agilent, S3022) for 45 minutes at room temperature ...
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bioRxiv - Pathology 2024Quote: ... Van-Gieson as a specific elastin stain or labelled with alpha-smooth muscle actin (a-SMA, Cat#M0851, Dako, 1:1000) as a specific marker for muscle ...
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bioRxiv - Neuroscience 2024Quote: ... 1 μL of derivatized sample was injected into a J&W HP-5ms Ultra Inert GC column (Agilent Technologies, #19091S-433UI) using a 1:10 split mode ...
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bioRxiv - Neuroscience 2024Quote: ... Then membranes were probed with the following antibodies: anti-human tau polyclonal antiserum KJ9A (1:5,000; A0024, Dako Cooperation, Carpinteria, CA) and rabbit anti-GAPDH antiserum (1:5,000 ...
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bioRxiv - Molecular Biology 2024Quote: The ATG (+1) codon of pKL-RLBP1 was mutated to an alanine codon (GCG) using the QuikChange Site-directed Mutagenesis kit (Agilent technologies) with the F primer and its reverse complement (Table S1 ...
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bioRxiv - Plant Biology 2024Quote: ... Fatty acid methyl esters (1 ml) were injected onto a DB-23 capillary column (60 m · 0.25 mm · 0.25 mm (Agilent Technologies, United States). A mix of FAME standards was used as references for peak identification by retention times ...
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bioRxiv - Plant Biology 2019Quote: ... RNA quality and integrity were evaluated by electrophoresis on 1% agarose gel and the RNA integrity number (RIN) was analysed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc.).
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bioRxiv - Microbiology 2019Quote: ... We extracted extracellular metabolites using solid phase extraction (SPE) with 1 g/6 cc PPL cartridges (BondElut, Agilent, Santa Clara, CA, USA) as described previously (Dittmar et al ...
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bioRxiv - Immunology 2021Quote: ... and bound serum IgG was detected by 70 µL/well of 1:3000 diluted rabbit anti-human IgG antibody linked to horseradish peroxidase (Agilent, P021402-2) incubated for 2h at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... The cDNA was diluted (1:10-20) and used for qRT-PCR in technical triplicate using Brilliant III SYBR master mix (Agilent Technologies, 600882) and CFX384 Touch Real-Time PCR Detection Systems (BioRad) ...
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bioRxiv - Immunology 2019Quote: ... overnight at 4 °C followed by a 1 h incubation with either an HRP-conjugated goat anti-mouse IgG or goat anti-rabbit IgG (Dako, Cambridge, UK). Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated for 2 hours at room temperature with a biotinylated goat anti-rabbit secondary antibody (Dako, 1:500 in blocking solution). Cells were washed 3 times and incubated for 30 minutes with streptavidin Cy3 (Sigma ...
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bioRxiv - Physiology 2019Quote: ... At least 10 sections per pancreas spaced at least 100 μm apart were stained with the following antibodies: Guinea pig anti-insulin (Dako, 1:500), Rabbit anti-proglucagon (Cell Signaling Technologies ...
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bioRxiv - Microbiology 2019Quote: ... Five mL of headspace gas sample from the 20 mL culture bottle was administered via a 1 mL GSV Loop to the GC-MS (Agilent 7890A/5975C). The volatile compounds were separated by CP7348 column (Agilent PoraBOND Q 25 m × 250 µm × 3 µm ...
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bioRxiv - Biochemistry 2019Quote: ... 1 μl of each PCR product was subjected to Chip-based capillary electrophoresis with the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA) and the proportional amount of each fragment to the whole yield of splicing products was calculated ...
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bioRxiv - Bioengineering 2021Quote: ... pCS2-GemininΔ27-GFP was generated by deleting amino acids 1-27 from Geminin-GFP [27] using QuikChange® Lightning mutagenesis kit (Agilent, 210513). The plasmids pCS2-Flag-FA-Geminin-GFP w.t and Δ27 variant were generated by cloning Geminin-GFP into pCS2-Flag-FA vector sing FseI and AscI restriction sites ...
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bioRxiv - Bioengineering 2021Quote: ... mRNA was isolated from high quality total RNA samples with RIN > 9 and 28S:18S > 1 (measured with an Agilent 2100 Bioanalyzer) using poly-T oligonucleotide beads ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent, Santa Clara ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent ...
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bioRxiv - Evolutionary Biology 2021Quote: ... a 2uL aliquot from each plated sample was diluted 1:9 on a Bravo liquid handling platform (Agilent, Santa Clara, CA, USA). A 5 μL aliquot from each diluted sample was arrayed into a 384 well plate for input into the mNGS library prep ...
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bioRxiv - Plant Biology 2021Quote: ... at 37⁰C (1:50 protein/trypsin ratio) and cleaned up with a C18 column (OMIX C18, Agilent, Santa Clara, CA, USA). Nano-liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were loaded onto a ZORBAX SB-C18 column (150 x 1 mm, 3.5 μm, flow of 90 μl/min; Agilent, Les Ulis, France) heated at 40°C ...
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bioRxiv - Neuroscience 2021Quote: ... A capillary column was the stationary phase (30 m x 0.53 mm x 1 µm film thickness; Agilent Technologies, Santa Clara, CA), and helium was used as the mobile phase (at a flow rate of 8.5 mL/min) ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibody detection was followed by 45 min of incubation with goat anti-mouse-biotin (DAKO, E0433, 1:200 in TNB blocking buffer) and subsequent incubation with Streptavidin-HRP Conjugate (PerkinElmer ...
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bioRxiv - Neuroscience 2019Quote: ... Endogenous peroxides were quenched with 1 % hydrogen peroxide in 10 % methanol for 10 minutes followed by blocking in antibody diluent (Dako /S 202230) for 10 minutes ...
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bioRxiv - Physiology 2019Quote: ... for 15 minutes and then incubated overnight at 4°C with primary antibodies diluted in DAKO antibody diluent: anti-Insulin (DAKO, 1:1000), anti-BRD4 (Bethyl laboratories ...
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bioRxiv - Bioengineering 2019Quote: ... followed by overnight staining with 1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and mouse-anti-CD31 (Dako; M082329-2) primary antibodies ...
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bioRxiv - Synthetic Biology 2020Quote: ... and was allowed to pass through the packed catalyst bed at space velocities in the range of 1-45 s−1 by helium as the diluent gas.44 The injection was immediately followed by separation using HP-PLOTQ column (Agilent, 19091P-QO4), and compounds in the effluent stream were identified using online Agilent 5975C Triple-Axis MS detector ...
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bioRxiv - Microbiology 2020Quote: ... Cells were next washed three times with PBS and incubated with a secondary fluorescein isothiocynate (FITC)-conjugated goat anti-mouse IgG (Dako; 1:200) in 1% BSA for 1 h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed with Bond Wash buffer 3 x 30s and then incubated with either mouse anti-CD68 (M0814, 1:100, Dako, Carpenteria, CA), mouse anti-βIII-Tubulin (G7121 ...
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bioRxiv - Neuroscience 2021Quote: ... the co-cultures were incubated first with primary antibody for glial fibrillary acidic protein (GFAP, 1:400, Z033429-2, Dako, Glostrup, Denmark) prepared in 5% NGS in DPBS overnight at +4 following an incubation with Alexa Fluor405 (A31556 ...
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bioRxiv - Physiology 2021Quote: ... Primary antibodies are diluted in 1x blocking solution and incubated with antibodies against insulin (1:2000, guineapig, Dako, cat. no. A-0546), glucagon (1:500 ...
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bioRxiv - Developmental Biology 2020Quote: ... and incubated for 30 minutes at room temperature with the secondary antibody (Supplementary Table 2)(1:500 diluted in DAKO Antibody Diluent). Cells were washed with 0.2% Triton X-100 in PBS ...
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bioRxiv - Microbiology 2021Quote: ... 190 to 601 nm (steps: 1 nm) and a Q-TOF (tandem mass spectrometry, quadrupole and Time of flight detector combined) (Agilent, Waldbronn, Germany). Electrospray ionization was performed in positive mode (ESI+ ...
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bioRxiv - Immunology 2021Quote: ... The membranes were washed and incubated with rabbit anti-goat IgG (500 ng ml−1, 5% milk in PBST; Dako, P044901-2), rabbit anti-mouse IgG (1.3 μg ml−1 ...
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bioRxiv - Microbiology 2021Quote: ... membranes were washed with 0.1% Tween-20 in PBS and incubated with anti-mouse or anti-human IgG (1:5000; Agilent Dako, Glostrup, Denmark), conjugated to horseradish peroxidase ...
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bioRxiv - Microbiology 2021Quote: ... were designed based on the DNA sequence for SARS-CoV-2 Wuhan-Hu-1 using the QuickChange Primer Design tool (Agilent Technologies, Inc.). Mutagenesis was carried out on a pCDNA-SARs2 Wuhan-Hu 1 S plasmid to create the P681H mutation ...
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bioRxiv - Immunology 2021Quote: ... and a 1:10 dilution of the resulting cDNA was run on a Bioanalyzer High Sensitivity DNA chip (Agilent Technologies #5067-4626) to assess cDNA quality and yield ...
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bioRxiv - Immunology 2021Quote: ... post-amplification and a 1:10 dilution of the resulting HTO libraries were run on a Fragment Analyzer (Agilent Technologies #5067-4626) to assess HTO quality and yield ...