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Citations for Agilent :
2451 - 2500 of 8085 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The Quick-Change site-directed mutagenesis kit (Agilent, Santa Clara, CA) was used to generate the specific point mutations within the coding regions of the GluN subunits ...
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bioRxiv - Neuroscience 2024Quote: ... The probes were labeled with Prime-It primer labeling kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... the RNA ScreenTape and D1000 ScreenTape kits (both from Agilent Technologies), Qubit RNA HS Assay kit ...
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bioRxiv - Genomics 2024Quote: ... and also using the High Sensitivity DNA Kit (Agilent, 5067-4626) with an Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2024Quote: ... The QuikChange Lightning Site-Directed Mutagenesis Kit was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Molecular Biology 2024Quote: ... using an RNA 6000 Nano kit (Agilent Technologies, cat# 5067-1511). Quality and integrity of extracellular RNA was evaluated by quality control PCR with a 1:10 diluted sample ...
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bioRxiv - Neuroscience 2024Quote: ... RNA quality was assessed using an RNA Pico 6000 kit (Agilent) and a Bioanalyzer 2100 (Agilent).
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bioRxiv - Genomics 2024Quote: ... using the High Sensitivity DNA analysis kit (Agilent, Cat# 5067-4626). ChIP-seq libraries were sequenced 75 bp single-end using Illumina NextSeq500/550 sequencer ...
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bioRxiv - Cancer Biology 2020Quote: ... blocked in 0.5% hydrogen peroxide for 5 minutes at room temperature and then blocked with 20% normal rabbit or goat serum (DAKO, Agilent, Cheadle, Cheshire, UK) for 20 min and incubated with the primary antibody overnight at +4°C ...
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bioRxiv - Neuroscience 2020Quote: ... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent, cat# Z031101-2 or between 1:4 and 1:5, Dako-Agilent, cat#IR50461-2) followed by 2h at RT with the chicken IgY anti-Neurofilament medium chain (NF-M ...
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bioRxiv - Biophysics 2021Quote: ... Mutations of key contact points between docked ligands and the human 5-HT5AR binding pocket were made via site-directed mutagenesis as directed (Stratagene, La Jolla, CA). Quickchange II mutagenic primer sets were the following ...
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bioRxiv - Microbiology 2021Quote: The determination conditions of bacillomycin D and fengycin were established by injecting 10 μL samples into an HPLC column (Eclipse XDB-C18, 5 μm; Agilent, Santa Clara, CA). The temperature was maintained at 30 °C during the experiment ...
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bioRxiv - Biophysics 2022Quote: ... 1 and 3.5 μm diameter fibers were manufactured from 2 and 5 wt% of high molecular weight polystyrene (MW: 15,000,000 g/mol, Agilent Technologies, Santa Clara, CA, USA) and equally spaced at approximately 60 μm ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples analyzed by mass spectrometry were resolved using a Poroshell StableBond 300 C8 (2.1 x 75 mm, 5 μm, Agilent Technologies part #660750-906) using a 1290 Infinity II UHPLC (G7120AR ...
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bioRxiv - Microbiology 2020Quote: ... The derivatized samples were analyzed on an Agilent GC-MS (GC model 7890A, MS Model 5975C) equipped with a (5% phenyl)-methylpolysiloxane capillary column (Agilent model HP-5MS). The injection port temperature was held at 280 °C and the oven temperature program was held at 80 °C for 1 min ...
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bioRxiv - Biochemistry 2021Quote: ... Analyses were performed on an Agilent Infinity 1260 HPLC system equipped with a 300 × 7.5 mm PLgel SE-HPLC column (particle size 5 μm, pore size 100 Å) (Agilent Technologies, Waldbronn, Germany). Dichloromethane was used as eluent at a flow rate of 1.00 ml/min ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli was carried out with a ZORBAX SB-Aq StableBond Analytical column (5 μm, 4.6 × 250 mm, Agilent Teologies, Santa Clara, CA, USA). A linear gradient of acetonitrile and water with 0.1% trifluoroacetic acid was used for development of the column at a flow rate of 0.8 mL/min ...
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bioRxiv - Genetics 2021Quote: ... Samples were frozen at -80 C until volatiles were separated using a DB-5 column on a Agilent 6890N gas chromatograph attached to an Agilent 5975 mass spectrometer (Agilent, Palo Alto, CA). Volatile quantitation was preformed using ChemStation software (Agilent ...
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bioRxiv - Biochemistry 2021Quote: ... (N-acetyl)paenilamicin-containing fractions were concentrated and purified subsequently by using a Grace HPLC column (GROM-Sil 120 ODS-5-ST, 10 µm, 250 × 20 mm) coupled to an Agilent 1100 HPLC system (Agilent Technologies, Waldbronn, Germany) with a MWD UV detector ...
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bioRxiv - Immunology 2022Quote: ... Quality control of the libraries to ensure no adapter dimers were present was carried out by examining 1ul of a 1:5 dilution on a High Sensitivity DNA chip (Agilent Technologies: 5067-4626) on an Agilent 2100 Bioanalyzer ...
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bioRxiv - Plant Biology 2022Quote: ... We analyzed 50 µL of each sample using an Agilent 1100-series HPLC machine with a diode array detector and a Zorbax Eclipse XDB-C18 column (4.6 x 150 mm, pore size 5 µm; Agilent Technologies, Santa Clara, USA). Desulfinated glucosinolates were separated at 40°C in a mobile phase beginning with 1.5% acetonitrile (ACN ...
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bioRxiv - Plant Biology 2022Quote: ... Samples of the final supernatant (5 μL) were automatically injected into a triple quadrupole (QqQ) LC-MS instrument (6430; Agilent Technologies, Waldbronn, Germany) equipped with an Eclipse Plus C18 column (2.1 × 50 mm ...
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bioRxiv - Plant Biology 2023Quote: ... Ten-μl B314-2 sample was injected into an Eclipse XDB-C18 column (250 mm x 4.6 mm, 5 μm, Agilent, Santa Clara, CA, USA) and eluted with the same gradient solvent system described above ...
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bioRxiv - Immunology 2024Quote: ... using an Agilent 7890B gas chromatograph equipped with a 30 m DB-35ms and 5 m Duraguard capillary column (Agilent, Santa Clara, California) for separation of derivatized metabolites ...
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bioRxiv - Biochemistry 2023Quote: ... Assays were performed on an Agilent 1100 series HPLC with a reverse phase column (Agilent Zorbax 300SB-C18, 5 μM, 2.1 × 150 mm). Solvent A was water containing 0.1% TFA and solvent B was acetonitrile containing 0.1% TFA ...
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bioRxiv - Microbiology 2023Quote: ... using two different carrier gases (argon and helium) and two stationary phases (CP- Molsieve 5 Å Plot and PoraPLOT U J&W GC columns, Agilent Technologies, Germany). Moreover ...
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). The liquid cultures were centrifuged at 3500 rpm for 5 minutes (Sorvall Legend X1R M20 rotor ...
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). 108 colony-forming units (CFUs ...
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Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... Coverslips were then incubated with 1X DAPI for 5 min before mounting on slides using DAKO mounting media (Agilent Technologies, Cat #S302380-2). Images were captured at 60X or 100X using a Nikon C2+ confocal microscope coupled to NIS-Elements AR software (Nikon).
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bioRxiv - Cell Biology 2023Quote: ... After washing for three times (5 mins) with H2O followed by three washing steps with Dako wash buffer (Agilent Technologies, Santa Clara, USA), antigen retrieval was done in a steamer using pH9 antigen retrieval from Dako ...
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bioRxiv - Genomics 2020Quote: ... Cells were sorted into G1 and G2M fraction based on Propidium iodide fluorescence using a MoFlo cell sorter (DakoCytomation).
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bioRxiv - Biochemistry 2019Quote: Cells were plated according to the manufacturer’s instructions in Seahorse cell culture plate (Agilent Technologies, Santa Clara CA, USA) in respective growth media ...
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bioRxiv - Cell Biology 2020Quote: ... smNPCs were split with accutase and 2×105 cells per well were seeded on Seahorse cell culture microplates (Agilent) one day before the ...
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bioRxiv - Cell Biology 2020Quote: ... lung fibroblasts were seeded at a concentration of 30,000 cells/well on XFp cell plates (Seahorse Bioscience, 103057-100) 24 hours prior to the initiation of studies ...
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bioRxiv - Neuroscience 2022Quote: ... Human iPSC-derived NGN2 neurons were plated on day 4 of dox induction at a density of 50,000 cells per well in the Seahorse XF96 cell culture microplates (Agilent), pre-coated with 20 µg/ml Laminin and 67 µg/ml Poly-ornithine (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: Real-time cell metabolic profiling of gastric cell lines was performed with a Seahorse XF HS Mini Analyzer (Agilent). Cells were seeded at the following densities ...
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bioRxiv - Immunology 2023Quote: ... at a density of 2×105 cells/well on a Seahorse XFe96 cell culture plate (Agilent Technologies; 101085-004). Plates were pre-coated with Corning™ Cell-Tak Cell and Tissue Adhesive (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... the cell culture of MEFs was started (1.6×104 cells/200 micro-l/well (Agilent, XF96 microplate V3-PS)) using a MEF medium ...
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bioRxiv - Developmental Biology 2024Quote: ... at a density of 9.1 × 105 cells/cm2 (mouse assay) or 7.3 × 105 cells/cm2 (human assay) in 100 μl of Seahorse XF DMEM (Agilent) supplemented with glucose (20 mM) ...
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bioRxiv - Cell Biology 2019Quote: ... First peptides were fractionated using high pH reverse phase chromatography on a C18 column (150 × 2.1 mm i.d. - Kinetex EVO (5 μm, 100 Å)) on a HPLC system (Agilent, LC 1260 Infinity II, Agilent). A two-step gradient was applied ...
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bioRxiv - Immunology 2021Quote: Gas chromatography was performed on an HP-5MS capillary column (5% benzene/95% methyl polysiloxane 30 m × 250 μm i.d., 0.25 μm film thickness, Agilent J & W Scientific, Folsom, CA, USA) with a constant flow of helium at 1 mL/min ...
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bioRxiv - Neuroscience 2020Quote: ... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent, cat# Z031101-2 or between 1:4 and 1:5, Dako-Agilent, cat#IR50461-2) followed by 2h at RT with the chicken IgY anti-Neurofilament medium chain (NF-M ...
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bioRxiv - Bioengineering 2022Quote: ... Cell nuclei were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI) for 5 min before being mounted using DAKO mounting medium (Agilent, catalog no. S302380–2). Imaging of the histological samples was performed on the Microscope Axio Imager.A2 (Carl Zeiss Microscopy ...
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bioRxiv - Biophysics 2021Quote: ... and endogenous peroxidase was blocked with 3% H2O2 in TBS for 5 min followed by incubation with anti-mouse EnVision+ labelled polymer (Agilent Technologies, Santa Clara, USA) for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... linear gradient from 5% to 35% ACN in 50 mM TEAAc pH 7 over 15 min at 50 °C, Agilent Technologies Series 1200 HPLC). The collected fractions were freeze dried 3 times ...
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bioRxiv - Synthetic Biology 2020Quote: LC-MS analysis was performed with the ZORBAX SB-Aq StableBond Analytical column (5 μm, 4.6 × 250 mm, Agilent Teologies, Santa Clara, CA, USA) on an Agilent 1260/6460 Triple-Quadrupole LC/MS system (Santa Clara ...
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bioRxiv - Immunology 2021Quote: ... TMT-labelled peptides were fractionated using high pH reverse phase chromatography on a C18 column (150 × 2.1 mm i.d. - Kinetex EVO (5 μm, 100 Å)) on a HPLC system (Agilent, LC 1260 Infinity II, Agilent). A two-step gradient was applied ...
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bioRxiv - Physiology 2021Quote: ... was amplified by PCR using cDNA of mouse kidney at the template with the primers 5′-CCGTCGACATGCAGGTCAAGAAATCCTG-3′ (SalI site in italics) and 5′-CCGGATCCTTAAAGGCGTGTGTGATCTT-3′ (M6 reverse primer; BamHI site in italics) and cloned into pBluescript SK(−) (Stratagene, La Jolla, CA, USA) using the SalI and BamHI sites ...
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bioRxiv - Physiology 2022Quote: ... The first was a DB 5 column (20 m long x 0.18 mm internal diameter x 0.18 μm thick) (Agilent J & W Scientific, Folsom, CA, USA), and the second a Rxi-17 column (0.84 m long x 0.1 mm internal diameter x 0.1 μm thick ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5 mM calcium chloride for 15 min at room temperature prior to recording fluorescence on a Citation 5 microplate reader (Agilent-BioTek, Santa Clara, CA), exciting at 488 nm and measuring emission at 535 nm ...