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Citations for Agilent :
2451 - 2500 of 2767 citations for NADH Cytochrome B5 Reductase 2 CYB5R2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... Real-time PCR amplification with the 2 x precision master mix (Primer design, Southampton, UK) using the Stratagene Mx3000P real-time QPCR system (Agilent Technologies, Santa Clara, CA, USA). Each sample was tested in triplicate and compared to a housekeeping gene (Atp5B in osteoblasts and bone tissue ...
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bioRxiv - Plant Biology 2022Quote: ... 2 μL of each sample was injected in splitless and split 1:10 mode into a 6890N gas chromatograph (Agilent Technologies Inc. Santa Clara, CA) coupled to a Pegasus 4D TOF mass spectrometer (LECO ...
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bioRxiv - Molecular Biology 2020Quote: ... and aromatic 1H-13C-NOESY-HSQC experiments with a mixing time of 150 ms each were carried out at 25 °C on a 500 MHz Agilent DirectDrive 2 spectrometer (Agilent Technologies, Santa Clara, CA, USA) equipped with a room temperature probe ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed again three times in TBS 1X and immunoreactions were revealed with DAB peroxidase substrate (K346811-2, Agilent Technologies, Santa Clara, CA, USA) was performed ...
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bioRxiv - Microbiology 2024Quote: ... Colorimetric conversion was terminated by addition of 5% SDS (50 uL per well) and absorbance was measured at 414 nm using a BioTek Epoch 2 Microplate Spectrophotometer (Agilent Technologies, Santa Clara, CA, USA). The endpoint absorbance of each sample was determined on a plate-by-plate basis where the absorbances of all the blank wells were averaged and multiplied by three ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed again three times in TBS 1X and immunoreactions were revealed with DAB peroxidase substrate (K346811-2, Agilent Technologies, Santa Clara, CA, USA). Finally ...
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bioRxiv - Biophysics 2023Quote: The samples were shortly centrifuged after thawing and injected onto a cooled (2°C) HPLC System (Agilent Infinity 1260, Agilent Technologies, Santa Clara, CA, USA) which includes a home packed pepsin column (IDEX guard column with 60 μl Porozyme immobilized pepsin beads ...
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bioRxiv - Pathology 2023Quote: ... Sections from the first level (main experiment) were stained for smooth muscle alpha-2 actin (ACTA2) and galectin 3 (LGALS3) using mouse monoclonal anti-ACTA2 (Dako, cat. no. M0851, 1:100) after blocking with Fab fragment (Jackson ImmunoResearch ...
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bioRxiv - Biochemistry 2023Quote: ... The fluorescence at 590 nm with excitation at 485 nm (F590) was measured with the BioTek Synergy 2 plate reader (Agilent Technologies, Santa Clara, CA, USA). The data were fitted using the Hill equation to estimate parameters from data which has the a priori unknown parameters ...
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bioRxiv - Cancer Biology 2021Quote: ... immunohistochemical staining was performed using an antibody directed against the mouse endothelial cell surface molecule CD31 (JC70A, DAKO, Glostrup, Denmark). 10 μm-thick cryosections were fixed with 4% PFA solution for 20 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... were incubated with the indicated primary antibodies (Reagents) overnight at 4 °C followed by Envision/diaminobenzidine detection (Dako, Glostrup, Denmark) and hematoxylin counterstaining/mounting (Entellan ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... then washed and incubated with Labelled polymer HRP-anti-Rabbit and Labelled polymer HRP-anti-mouse secondary antibody (Dako, Denmark) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... then probed for 1 hr at room temperature with HRP-conjugated goat anti-mouse IgG secondary antibodies 1:500 in PBS (Dako, Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated with secondary antibody polyclonal rabbit anti-mouse HRP-conjugate (cat.P0260, Dako, Agilent Technologies, Santa Clara, CA, USA) diluted 1:100 in PBS 1× for 1h at room temperature (RT) ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 drops per slide of secondary antibody (EnVision + labeled polymer-HRP anti-mouse, Dako K4001, or anti-rabbit, Dako K4003) were added and incubated for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 drops per slide of secondary antibody (EnVision + labeled polymer-HRP anti-mouse, Dako K4001, or anti-rabbit, Dako K4003) were added and incubated for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... The REAL™ EnVision™ Detection system including anti-rabbit/mouse IgG secondary antibodies and peroxidase/diaminobenzidine (Dako, Agilent Technologies) was used to visualize the primary antibody‑binding cells according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The REAL™ EnVision™ Detection system including anti-rabbit/mouse IgG secondary antibodies and peroxidase/diaminobenzidine (Dako, Agilent Technologies) was used to visualize the primary antibody‑binding cells according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Endogenous peroxidases were blocked with peroxidase inhibitor (CM1) for 8 minutes before incubating the section with CD3 antibody (Dako, #A0452) at 1:300 dilution for 60 minutes at room temperature ...
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bioRxiv - Pathology 2022Quote: ... Anti-CD138 antibody was used for detection of plasma cells by immunohistochemistry (MI15 clone; Agilent Dako, Santa Clara, California, USA) at 1:25-1:50 dilution range.
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bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated secondary antibodies were then incubated on slides for 1-hour and detected with the DAB+ chromogen kit (DAKO). After washing ...
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bioRxiv - Developmental Biology 2019Quote: ... Brain sections that were stained with antibodies that required antigen retrieval were incubated in Dako 1X target retrieval solution (Agilent Dako) or sodium acetate buffer ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated overnight at 4°C with primary antibodies (rabbit anti-TMEM98, Proteintech, 1:1000; rabbit anti-FLAG polyclonal, Sigma, 1:2000; mouse anti-FLAG monoclonal M2, Stratagene 1:2000 ...
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bioRxiv - Immunology 2019Quote: ... Visualization of bound anti-insulin antibody was achieved using HRP-labelled polymer-conjugated goat anti-rabbit IgG (Dako EnVision+ System), followed by counterstain with hematoxylin ...
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bioRxiv - Immunology 2019Quote: ... Epithelial cells in the EDTA fraction were depleted by incubation with anti-human epithelial antigen antibody (clone Ber-EP4, Dako) followed by anti-mouse IgG dynabeads (ThermoFisher ...
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bioRxiv - Immunology 2019Quote: ... incubated overnight at 4 °C with polyclonal guinea pig anti-insulin antibody (1:100 in 1% BSA in DPBS, DAKO) followed by 1 h room temperature incubation either with Alexa Fluor® 488 AffiniPure donkey anti-guinea pig or with DyLight™ 594 AffiniPure donkey anti-guinea pig secondary antibody (1:100 in 1% BSA in DPBS ...
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bioRxiv - Immunology 2019Quote: ... Immunofluorescence staining was carried out using the following antibodies: a primary rabbit anti-GFAP (resting and reactive astrocytes, 1:500 dilution, Z0334, Dako), and a primary rabbit anti-Iba1 (resting and activated microglia/macrophages ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The multiple charged peaks of the antibody were deconvoluted using the Agilent MassHunter Bioconfirm software (deconvolution for protein, Agilent technology) with the deconvolution range from 20 kDa to 160 kDa ...
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bioRxiv - Neuroscience 2020Quote: ... IdU) on alternate one-in-ten sections using different anti-BrdU antibodies from different vendors (for BrdU: 1/200, Dako, Glostrup ...
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bioRxiv - Cancer Biology 2021Quote: HLA-restriction of antigen recognition was tested in anti-IFN-γ ELISpot using autologous DCs loaded with the relevant peptide or without peptide (negative control) and blocking antibodies against HLA class I and II (both Dako). DCs were incubated with peptides overnight and the next day anti-HLA mABs were added to ELISpot plates for 1 hour prior to the addition of expanded cells at a ratio of 1 DC to 50 expanded cells ...
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bioRxiv - Neuroscience 2020Quote: ... and retrovirus expressing EGFP in alternating one-in ten sections using different anti-BrdU antibodies from different vendors (for BrdU: mouse primary at 1/100, Dako; CldU ...
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bioRxiv - Cancer Biology 2020Quote: ... to avoid cross-reaction during the incubation of the following cocktail of primary antibodies: mouse monoclonal anti-pan-cytokeratin (clones AE1/AE3, DAKO) to detect the epithelial compartment ...
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bioRxiv - Neuroscience 2021Quote: ... A corresponding anti-rabbit or anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody at a dilution of 1:7000 (Dako) or 1:2000 (Pierce ...
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bioRxiv - Cancer Biology 2020Quote: ... The slides were washed the next day and then incubated with biotinylated anti-rabbit secondary antibody using a DAB kit (Dako) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... 0.2% Triton X-100 and incubation with appropriate secondary antibody for 2h at room temperature in blocking buffer before washing and mounting in fluorescent mounting medium (DAKO). Images were acquired using a Leica SP8 confocal microscope.
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bioRxiv - Immunology 2020Quote: ... The S228P amino acid change in the anti-MuSK antibodies was achieved by converting AGC>CCC through side-directed mutagenesis based on the QuikChange II system (Agilent). To make the b12 antibody suitable as an exchange partner for cFAE ...
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bioRxiv - Physiology 2021Quote: ... sections were incubated with secondary HRP coupled antibodies followed by addition of DAB+ Substrate Chromogen System (Dako Omnis, Glostrup, Denmark). For each experiment ...
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bioRxiv - Cancer Biology 2021Quote: ... for 30 min or with 0.4 µg/ml anti-human cath-D mouse monoclonal antibody (clone C-5) for 20 min after heat-induced antigen retrieval with the PTLink pre-treatment (Dako) and the High pH Buffer (Dako ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were incubated overnight with primary antibodies (collagen I; rabbit anti-bovine IgG, Cederlane, Burlington, ON, dilution 1:500, collagen IV; mouse anti-human IgG, Dako, Carpinteria ...
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bioRxiv - Pathology 2022Quote: ... appropriate HRP-coupled secondary antibodies (rabbit anti-mouse, Cat. No. P0260, and goat anti-rabbit, Cat. No. P0448, from DAKO and rabbit anti-goat ...
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bioRxiv - Cell Biology 2022Quote: ... Non-specific antibody binding was blocked by incubation for 30 min with blocking solution containing 5% normal goat serum (NGS, Dako) at room temperature followed by overnight incubation at 4 °C with different primary antibodies listed in Table 1 ...
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bioRxiv - Bioengineering 2022Quote: ... of primary antibody with competitor solution was transferred to array hybridization cassettes (Fig. S5) using a Bravo automated liquid handling platform (Agilent) with a 96-channel head ...
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bioRxiv - Neuroscience 2022Quote: ... and the presence of the secondary antibody was revealed by a streptavidin–horseradish peroxidase conjugate using diaminobenzidine (Dako, Glostrup, Denmark). Sliced were counterstained with Harris hematoxylin.
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bioRxiv - Molecular Biology 2022Quote: ... the blots were further incubated with a primary anti-total Tau antibody (rabbit Dako-A0024, 1:5000 in blocking buffer), then with the anti-rabbit HRP-conjugated secondary antibody ...
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bioRxiv - Pathology 2022Quote: ... Anti-CD138 antibody was used for detection of plasma cells by immunohistochemistry (MI15 clone; Agilent Dako, Santa Clara, California, USA) at 1:25-1:50 dilution range.
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bioRxiv - Physiology 2022Quote: ... Membranes were washed with TBS + 0.1% Tween 20 and then incubated for 1h at RT with the following secondary antibodies diluted in blocking buffer: swine anti-rabbit Ig HRP-conjugated (1:3000, catalog no. P0399, Dako) or goat anti-mouse Ig HRP-conjugated (1:5000 ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were washed three times with TBS-T and incubated for 1 hour with HRP-conjugated secondary antibodies (Dako). Proteins were detected by enhanced chemiluminescence reagents (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... The membranes were washed and incubated with secondary swine anti-rabbit HRP conjugated antibody (Agilent DAKO, Santa Clara, CA, P0399) in 1% milk in PBST for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... three 5 μm-thick tissue sections separated by 300 μm were stained with guinea pig anti-insulin antibody (undiluted, Dako IR002 ...
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bioRxiv - Neuroscience 2021Quote: Mouse brain cryosections for studying transmitophagy in vivo were blocked with 10% normal goat serum for 30 minutes at room temperature and incubated with an anti-GFAP primary antibody (Dako, Z033429-2 ...