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Citations for Agilent :
201 - 248 of 248 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... samples were injected into a C18 reverse phase column (Atlantis T3, 2.1 × 150 mm, 3 μm; Waters) using HPLC (Agilent 1290 LC) at a flow rate of 0.15□ml/min with 5 mM ammonium formate for mobile phase A and 100% methanol for mobile phase B ...
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bioRxiv - Microbiology 2024Quote: ... Separation was done on a Poroshell 120 Phenyl Hexyl column (150 × 2.1 mm i.d., 1.9 μm; Agilent Technologies, Santa Clara, CA) maintained at 40°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Separation was achieved on an Agilent InfinityLab Poroshell 120 HILIC-Z column (2.1 mm x 150 mm, 2.7 µm particle size, PEEK-lined) (Agilent Technologies, Waldbronn, Germany). Different eluents and gradients were applied for positive and negative ionization modes ...
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bioRxiv - Bioengineering 2023Quote: ... equipped with a UV detector through a C18 column Poroshell 120 EC-C18 (4.6 × 150 mm, 4 μm) (Agilent Technologies, Inc.). Mobile phase consisted of acetonitrile/methanol/0.4% acetic acid/ tetrahydrofuran (80:12:5:3 ...
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bioRxiv - Biophysics 2023Quote: ... The monodisperse sample solutions of proteins were injected onto a size exclusion column (David and Perez 2009) (SEC-3, 150 Å; Agilent) using an Agilent HPLC system and eluted into the capillary cell at a flow rate of 0.3 ml min-1 ...
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bioRxiv - Neuroscience 2023Quote: ... Chromatography was carried out using a Zorbax SB C18 column (150 × 2.1 mm, 5 µm, Zorbax, Agilent, Santa Clara, CA, USA) which was proceeded with a SB-C18 Guard Cartridges (12.5 × 2.1 mm ...
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bioRxiv - Cancer Biology 2023Quote: ... with a Poroshell 120 EC-C18 column (150 mm × 2.1 mm, 2.7 μm particle size, Agilent Infinity Lab, Santa Clara, CA) was employed using a gradient of solvent A (90%:10% H2O ...
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bioRxiv - Molecular Biology 2023Quote: ... Agilent 1290 Infinity II LC system and separated using a 150 mm Agilent AdvanceBio SEC 2.7 micron HPLC Bio size-exclusion column (Agilent #PL1580-3301). The mobile phase used was 100 mM Sodium Phosphate ...
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bioRxiv - Molecular Biology 2024Quote: ... Jurkat T cells in 1% BSA/RPMI media were exposed to 150 mJ/cm2 of irradiation using the Stratagene UV Stratalinker 1800 (Agilent Technologies) and incubated for 4 hours at 37°C ...
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bioRxiv - Neuroscience 2024Quote: Proteomic analyses were performed by LC-qTOF-MS on a C18 column (150 × 1 mm, 3.5 μm, 300 Å, Agilent, USA) with buffer A (0.1% formic acid in water ...
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bioRxiv - Cancer Biology 2024Quote: ... then triethylammonium phosphate monobasic solution - CH3CN from 0:100 to 90:10 in 5 min with a flow rate of 1 mL/min (Column: Zorbax SB-Aq 5 µm analytical column, 150 X 4.6 mm; Agilent Technologies, Inc). Method C ...
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bioRxiv - Cancer Biology 2024Quote: ... then triethylammonium phosphate monobasic solution - CH3CN from 20:80 to 80:20 in 10 min with a flow rate of 1 mL/min (Column: Zorbax SB-Aq 5 µm analytical column, 150 X 4.6 mm; Agilent Technologies, Inc). Peaks were detected by UV absorption (210 nm ...
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bioRxiv - Biochemistry 2024Quote: ... Liquid chromatography on an Agilent Pursuit 5 Diphenyl 150 x 2.0 mm column with tandem mass spectrometry (LC-MS) confirmed the peptide mass and purity (Agilent, California, USA). LC and MS for all peptides can be found in SI Figures S1-S8 ...
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bioRxiv - Microbiology 2020Quote: ... The purity of each subfraction was analysed by reversed phase HPLC (COSMOSIL Packed C18 column, 4.6 ID × 150 mm, Agilent Technologies 1260 infinity). The following mobile phase was used ...
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bioRxiv - Microbiology 2020Quote: ... of sample was loaded via the autosampler onto a C18 peptide column (AdvanceBio Peptide 2.7 um, 2.1 x 150 mm, Agilent part number 653750-902) enclosed in a thermostatted column oven set to 50 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were loaded onto a ZORBAX SB-C18 column (150 x 1 mm, 3.5 μm, flow of 90 μl/min; Agilent, Les Ulis, France) heated at 40°C ...
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bioRxiv - Microbiology 2021Quote: ... depleted serum were eluted from a high-capacity nano-HPLC Chip (160 nL, 150 mm × 75 μm ID, Agilent, Santa Clara, CA) with a 30 min gradient of 3-40% acetonitrile as described previously 28,29 ...
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bioRxiv - Biochemistry 2020Quote: ... SpeB digests were separated on an AdvanceBio Diphenyl column featuring superficially porous particles (150 × 2.1 mm i.d., 3.5 μm particle size, 450 Å pore size, Agilent, Santa Clara, CA, USA) at a flow rate of 200 µL.min-1 and a column oven temperature of 80 °C ...
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bioRxiv - Genomics 2020Quote: Dried peptides were separated by high pH reverse phased liquid chromatography on an Agilent 1100 HPLC system using a Zorbax Eclipse column (2.1 × 150 mm, 5 um) (Agilent Technologies, Palo Alto). Peptides were eluted with a linear gradient of 20 mM ammonium formate pH 10 ...
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bioRxiv - Plant Biology 2022Quote: ... mounted to an Agilent 6890N gas chromatograph with split/splitless injector and electronic pressure control up to 150 psi (Agilent, Böblingen, Germany). Mass spectrometric data were acquired through a Pegasus III time-of-flight mass spectrometer (LECO Instrumente GmbH ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatants were fractionated on a reversed-phase Supelco Discovery BIO wide Pore C18-3 (4.6 × 150 mm, 3 µm particle size) column operated by a HPLC Agilent 1200 (Agilent Technologies, Waldbronn, Germany) chromatography system ...
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bioRxiv - Bioengineering 2023Quote: ... bEnd.3 cells were seeded at a density of 6×103 cells/well in rat tail collagen-coated (150 µg/mL) 96-well plates (E-plate 96; Agilent, cat# 300600910) and confluent monolayers were treated as described above for human ECs ...
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bioRxiv - Biochemistry 2024Quote: ... The separation was carried out on Zorbax Extend-C18 (4.6 x 150 mm, 3.5 μm) column (Agilent Technologies, Santa Clara, CA, USA) with mobile phase consisting of 5 mM ammonium bicarbonate in water as solvent A and acetonitrile as solvent B applying the following gradient (% B) ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were loaded onto a ZORBAX SB-C18 column (150 x 1 mm, 3.5 μm, flow of 90 μl/min; Agilent, Les Ulis, France) heated at 40°C ...
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bioRxiv - Plant Biology 2023Quote: ... Chromatographic separations were performed on an Agilent InfinityLab Poroshell 120 HILIC-Z column (2.7 μm, 2.1 mm × 150 mm, Agilent Technologies, Santa Clara, CA) at a column temperature of 50°C and a flow rate of 0.8 mL/min ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 ng of purified RNA was mixed with RNA standards (One Colour RNA Spike-In Kit; Agilent, Santa Clara, CA – 5188–5282) and the mixture labelled with LowInput QuickAmp Labeling Kit One-Color (Agilent 5190-2305) ...
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bioRxiv - Physiology 2024Quote: ... labelled peptides were pre-fractionated in order to reduce sample complexity using a 150 mm C18 column on an Agilent 1260 Infinity II high performance liquid chromatography instrument (Agilent Technologies, USA). Gradient elution was used to collect 24 fractions in 60 min ...
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bioRxiv - Biochemistry 2024Quote: ... coupled to a Vanquish HPLC system (Thermo) using an InfinityLab Poroshell 120 HILIC-Z column (2.1 × 150 mm, 2.7 μm, Agilent catalogue number 673775-924). The injection volume was 5 μl ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were plated in 150 μL of complete DMEM at 1 × 105 cells/well on 24-well Seahorse plates (Agilent Technologies, USA) 24 h prior to the assays ...
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bioRxiv - Cell Biology 2024Quote: ... Fragmentation of chromatin to an average size of 150-500bp was checked on Agilent 2100 Bioanalyzer using High Sensitivity DNA Kit (Agilent, 5067-4626). To reduce the SDS ...
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bioRxiv - Biophysics 2021Quote: ... incubated for 16 h at 4 °C and purified by HPLC on a Zorbax C3 column with dimensions of 21 × 150 mm (Agilent, Santa Clara, CA) using a gradient of 20-90 % acetonitrile in water with 0.1 % trifluoroacetic acid (TFA ...
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bioRxiv - Plant Biology 2022Quote: ... We analyzed 50 µL of each sample using an Agilent 1100-series HPLC machine with a diode array detector and a Zorbax Eclipse XDB-C18 column (4.6 x 150 mm, pore size 5 µm; Agilent Technologies, Santa Clara, USA). Desulfinated glucosinolates were separated at 40°C in a mobile phase beginning with 1.5% acetonitrile (ACN ...
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bioRxiv - Biochemistry 2023Quote: ... Assays were performed on an Agilent 1100 series HPLC with a reverse phase column (Agilent Zorbax 300SB-C18, 5 μM, 2.1 × 150 mm). Solvent A was water containing 0.1% TFA and solvent B was acetonitrile containing 0.1% TFA ...
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bioRxiv - Immunology 2021Quote: ... TMT-labelled peptides were fractionated using high pH reverse phase chromatography on a C18 column (150 × 2.1 mm i.d. - Kinetex EVO (5 μm, 100 Å)) on a HPLC system (Agilent, LC 1260 Infinity II, Agilent). A two-step gradient was applied ...
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bioRxiv - Microbiology 2024Quote: ... Lipid samples were reconstituted in 150 µL isopropyl alcohol and transferred to an LC-MS vial with insert (Agilent 5182-0554 and 5183-2086) for analysis ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were analyzed using an Aglient 1290 series HPLC installed with a HILIC-Z column (150 mm ×2.1 mm, 2.7 μm, 120 Å, Agilent Technologies, Santa Clara, CA, USA). The aqueous and organic mobile phases were LC-MS grade water with 5 mM ammonium acetate and 0.2% acetic acid (A ...
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bioRxiv - Cancer Biology 2024Quote: ... The analytes were separated on a Sciex C18 column (4.6 mm × 150 mm, 5 μm) maintained at 50°C using a 1260 Infinity HPLC instrument (Agilent Technologies, Santa Clara, CA, USA). A gradient flow of the mobile phase was applied ...
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bioRxiv - Synthetic Biology 2020Quote: ... Fractions containing the target peaks were combined and further purified by a Reveleris flash chromatography system (Grace) using a C18 Preparative column (Agilent, 5 μm, 21.2 × 150 mm). A gradient method (55% acetonitrile-water to 85% acetonitrile-water in 12 mins ...
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bioRxiv - Immunology 2021Quote: ... The infected cell monolayers were rinsed twice with PBS and then irradiated with 150 mJ/cm2 of UV light using a Stratalinker 1800 (Agilent technologies, Santa Cruz, CA, USA) to induce apoptosis ...
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bioRxiv - Molecular Biology 2020Quote: ... and aromatic 1H-13C-NOESY-HSQC experiments with a mixing time of 150 ms each were carried out at 25 °C on a 500 MHz Agilent DirectDrive 2 spectrometer (Agilent Technologies, Santa Clara, CA, USA) equipped with a room temperature probe ...
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bioRxiv - Cell Biology 2022Quote: ... from mitochondrial samples (0.150 mg/mL) in the respiration buffer under constant stirring at 28°C using a Cary Eclipse Fluorescence Spectrophotometer (Agilent Technologies, Santa Clara, CA, USA) as described previously (Emaus et al. ...
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bioRxiv - Genomics 2023Quote: RNA seqFISH+ and two-layer DNA seqFISH+ primary probes were generated from oligo array pools (Twist Bioscience for 150-nt oligos; Agilent Technologies for 170-nt oligos) based on Oligopaint technologies80 with enzymatic amplifications as described previously7 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated with the required primary antibody (ABri: 338 1:1000 from Ghiso lab; ADan: 5282 1:1000 from Ghiso lab; CD68: 1:150 DAKO; CR3/43: 1:100 DAKO) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... from mitochondrial samples (0.150 mg/mL) in the respiration buffer under constant stirring at 28°C using a Cary Eclipse Fluorescence Spectrophotometer (Agilent Technologies, Santa Clara, CA, USA) as described previously [49].
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bioRxiv - Microbiology 2021Quote: The substrates and cleavage products were analyzed on a 1260 Infinity II Agilent technologies HPLC equipped with an Infinitylab Poroshell 120 C18 analytical column (Agilent technologies, 4.6 x 150 mm, 4µm). Eluent A was 50 mM ammonium phosphate buffer pH 6.8 and eluent B was 50% methanol in water ...
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bioRxiv - Biochemistry 2023Quote: ... Metabolomics data were acquired using an Agilent Infinity II/6495 LC-MS/MS fitted with an InfinityLab Poroshell 120 HILIC-Z column (Agilent, 2.1 x 150 mm, 2.7 Micron). The mass spectrometer was operated in both positive and negative ion modes using polarity switching ...
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bioRxiv - Cancer Biology 2024Quote: ... flowing 2 mM NH4CHO2 (Solvent A) and ACN (Solvent B) at 15 µL/min through a Zorbax SB-C18 column (Agilent, 0.5 x 150 mm, 3 µm). Quantitation was done monitoring MS/MS transitions m/z 242.1 [M + H]+ → m/z 126.1 [M – deoxyribose + H]+ for 5mC ...
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bioRxiv - Systems Biology 2024Quote: Extracts were separated on an Agilent 1290 Infinity II Bio LC System using an InfinityLab Poroshell 120 HILIC-Z column (Agilent 683775-924, 2.7 µm, 2.1 x 150 mm), maintained at 15°C ...