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Citations for Agilent :
201 - 250 of 685 citations for Recombinant Human STAT1 His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... R448A and N455A were introduced in the pETNKI-his-JBP1 JDBD plasmid using a modified QuickChange site-directed mutagenesis method (Agilent). Forward and reverse mutagenesis primers containing the single point mutations were purchased from ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... 500-1000ng of Hi-C library were captured using the SureSelectXT Target Enrichment System for the Illumina Platform (Agilent Technologies) as instructed by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant adenovirus particles were produced following the AdEasy XL Adenoviral Vector System protocol (#240010 Agilent Technologies). Islets were transduced using a microfluidic device to obtain uniform infection of the islet cells throughout the islet volume and were incubated overnight before imaging ...
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bioRxiv - Genomics 2019Quote: Same amounts of early and late-labeled DNA were loaded on mouse or human DNA microarrays (SurePrint G3 Human CGH arrays, Agilent Technologies, G4449A). Hybridization was performed as previously described (Hadjadj et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Same amounts of early and late-labeled DNA were loaded on human DNA microarrays (SurePrint G3 Human CGH arrays, Agilent Technologies, G4449A). Hybridization was performed as previously described (39) ...
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bioRxiv - Biochemistry 2023Quote: ... Same amounts of early and late-labelled DNA were loaded on human DNA microarrays (SurePrint G3 Human CGH arrays, Agilent Technologies, G4449A). Hybridization was performed at 65°C as previously described43 ...
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bioRxiv - Systems Biology 2019Quote: ... Translating mRNA bound to the GFP-tagged ribosomal subunit protein L10a was then pulled-down and purified (Absolutely RNA Microprep Kit, Agilent Technologies, Wilmington, DE). RNA-Seq libraries were prepared from 1 μg of purified RNA using the NEBNext Ultra RNA Library Prep Kit for Illumina with NEBNext Poly(A ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA was purified from total RNA using biotin-tagged poly dT oligonucleotides and streptavidin-coated magnetic beads and quality was assessed using an Agilent Technologies 2100 Bioanalyzer (Agilent, Santa Clara, CA).
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bioRxiv - Cell Biology 2020Quote: ... For the anti-human Fibronectin immunostaining (Dako, 1:1000) a single immunostaining was performed with a goat anti-rabbit secondary antibody and Alexa-568 phalloidin ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-human Chorionic Gonadotropin (hCG, A0231, Dako, 1:300), anti-ENDOU (HAP067448 ...
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bioRxiv - Genetics 2019Quote: ... using the Cy3-labeled Universal Human Reference cRNA (Agilent). Genes that showed significant change of expression (normalized to DMSO control-treated samples ...
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bioRxiv - Immunology 2019Quote: ... and rabbit anti-human myeloperoxidase (A039829-2, Dako, USA) at 1:300 ...
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bioRxiv - Developmental Biology 2019Quote: ... or mouse anti-human CD31 (Dako, M0823, 1:50) overnight at 4°C and then goat anti-mouse Alexa488 (ThermoFisher Scientific ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... and with rabbit anti-human IgM (1:250, Dako). Incubation with primary antibodies was followed by a biotinylated goat-anti-rabbit antibody for 30 min followed by the avidin–biotin–peroxidase complex (Vectastain Elite ABC Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... A Universal Human Reference (UHR) (Chem-Agilent, Catalog #740000) was sequenced with each batch of samples to allow for assessment and removal of technical artifacts (due to ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA samples were performed to Microarray analysis by Agilent SurePrint G3 human gene expression Microarray 8X60K (Agilent Technologies). After hybridization ...
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bioRxiv - Genomics 2022Quote: Universal Human Reference RNA (UHRR) was purchased from Agilent (product name ...
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bioRxiv - Genetics 2022Quote: Preparation of the universal human reference RNA (Agilent, 74000) was done according to the manufacturer’s manual ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... A monoclonal mouse anti-human αSMA antibody (Dako, Denmark) was incubated for 2 hours at room temperature after antigen retrieval ...
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bioRxiv - Cancer Biology 2020Quote: ... Library preparation (Agilent SureSelect Human All Exon V6 kit) and high throughout sequencing (Illumina NovaSeq 6000 ...
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bioRxiv - Cell Biology 2021Quote: ... and IgA (rabbit anti-human, Dako, A0262, 1:75). For amplified targets the appropriate HRP-conjugated secondary was then incubated at 1:250 in PBS for 45 minutes-1hr followed by TSA fluorophore treatment (Akoya) ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-human Chorionic Gonadotropin (CBG, A0231, Dako, 1:1000), anti-ENDOU (HAP067448 ...
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bioRxiv - Cancer Biology 2020Quote: ... tumor sections were stained for human Ki67 (Dako, #M7240) using the Target Retrieval Solution pH 9.0 (Dako ...
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bioRxiv - Microbiology 2022Quote: ... Sections were stained with mouse anti human CMV (Dako, Agilent technologies ...
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal CD31 anti-human clone JC70A (M0823, Agilent Dako ...
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bioRxiv - Neuroscience 2022Quote: ... wild type human TTC3 in pCMV-Tag2 vector (Stratagene), mouse AUTS2-myc-DDK in pCMV6 (OriGene ...
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bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-human CD31 (Agilent, clone DAKO JC70A), mouse monoclonal anti-HLA class II-DR/DP/DQ (Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-human CD31 (Agilent, clone DAKO JC70A), mouse monoclonal anti-HLA class II-DR/DP/DQ (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... and immunolabelled for human CD31 (Dako, JC70A, 1:50), VE-Cadherin (1:100 ...
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bioRxiv - Immunology 2023Quote: ... human albumin (diluted 1:1000; Dako, Carpinteria, CA, USA), complement C3 (diluted 1:1000 ...
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bioRxiv - Genomics 2020Quote: ... HindIII-based Hi-C libraries from our previous study (Wutz et al., 2017) were captured using SureSelect target enrichment system (Agilent Technologies) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... the eight genes flanked with the same promoters and terminators were cloned into the 2μ-based high-copy plasmids pESC-URA-USER and pESC-HIS (#217451, Agilent Technologies), resulting in pESC-URA-USER harboring CYP79A2 ...
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bioRxiv - Biophysics 2020Quote: ... Human SHP-1 with an N-terminal 6x His tag was produced in Escherichia coli BL21-CodonPlus (DE3)-RIPL strain (Agilent Technologies) and purified on Ni2+-NTA agarose (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... Each Hi-C library (750 ng) was hybridized and captured individually using the SureSelectXT Target Enrichment System reagents and protocol (Agilent Technologies). After library enrichment ...
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bioRxiv - Evolutionary Biology 2021Quote: Evolved viral genomes of the passage 12 from each lineage and genotype were amplified by high-fidelity RT-PCR using the AccuScript Hi-Fi (Agilent Technologies) reverse transcriptase and Phusion DNA polymerase (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were subjected to C18 desalting using an Agilent 1260 Infinity LC system equipped with an MRP-C18 Hi-recovery column (Agilent, USA) before SpeedVac drying.
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bioRxiv - Microbiology 2022Quote: ... and analysed by HPLC Shimadzu, Prominence-I (Milton Keynes, UK) with an ion exchange column Hi PlexH (300 × 7.7 mm) (Agilent, Cheadle, UK) and 0.6 mL min-1 flow rate at 55 ºC (oven temperature ...
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bioRxiv - Genomics 2023Quote: The fragment size distribution and concentration of the final PacBio and Dovetail Hi-C libraries were assessed using the TapeStation (Agilent Technologies) and the Qubit Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The pST39-BLOC-1 plasmid encoding recombinant BLOC-1 was transformed into BL21gold(DE3)plysS cells (230134, Agilent). Several colonies from the plate were inoculated into a starter culture of 10 ml LB supplemented with 34 μg/ml chloramphenicol and 100 μg/ml ampicillin that was grown overnight at 37 °C with moderate shaking ...
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bioRxiv - Cell Biology 2021Quote: All recombinant proteins were expressed in either BL21-CodonPlus (DE3)-RIPL or ArcticExpress (DE3) competent cells (Agilent Technologies) grown in LB medium overnight at 13-16°C ...
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bioRxiv - Neuroscience 2023Quote: Recombinant adeno-associated viruses (AAVs) with serotype 8 were produced using the AAV helper-free system (Agilent Technologies). Human embryonic kidney (HEK ...
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bioRxiv - Neuroscience 2023Quote: Plasmid construct (pRK172-human-lil-synuclein) encoding WT-human-α-synuclein was expressed in BL21-CodonPlus (DE3-RIL) cells (Agilent, Cat# 230245-41). Protein expression was induced with 0.1 mM IPTG at cell density OD (600 nm ...
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bioRxiv - Bioengineering 2021Quote: ... and MBP-tagged (for immunoblotting) NC1 domains of type IV Collagen α1-6 (V) were expressed in BL21-CodonPlus-RP (Agilent Technologies, Santa Clara, CA) transformed with pET-28a (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Rabbit anti-human tau was from Dako (1:8000, A0024). Other tau antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunohistochemistry (IHC) for human CD45 antibodies (IR75161-2, Agilent Technologies) was performed on FFPE blocks of engrafted tumors to identify cases of lymphomagenesis ...
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bioRxiv - Immunology 2022Quote: ... the plates were incubated with either anti-human-HRP (Dako), anti-rat-HRP (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... (30) using the SureSelectXT Human All Exon 50 Mb (Agilent) bait set ...
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bioRxiv - Cancer Biology 2020Quote: ... monoclonal mouse anti-human alpha smooth muscle actin (M0851, Dako), monoclonal mouse anti-human CD68 (M0814 ...
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bioRxiv - Cancer Biology 2021Quote: ... or 1:400 diluted Rabbit anti-human calcitonin (Dako, Denmark) at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Samples were analyzed against the Stratagene Universal Human Reference (Agilent). Raw fluorescence intensities were quantified and normalized (Lowess normalization ...