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Citations for Agilent :
201 - 248 of 248 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Hippocampal neurons were seeded in a poly-d-lysine-coated XF 96-well cell culture microplate (Seahorse Bioscience, Copenhagen, Denmark) in triplicate at 5 × 105 cells per well in 1 ml neuronal growth medium and then incubated at 37 °C in 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a nitrocellulose membrane before probing using a primary anti IL-36γ antibody (Biotechne (R&D) UK) overnight at 4°C and a secondary goat antibody (Dako) for 1 hour at room temperature to visualise any protein cleavage.
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bioRxiv - Immunology 2021Quote: ... 3×105 CD8+ T cells were plated onto poly-D-lysine coated wells and assayed in XF RPMI medium (Agilent) pH 7.4 supplemented with 10 mM glucose and 2 mM glutamine ...
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bioRxiv - Plant Biology 2020Quote: ... Eluting metabolites were fragmented in electron impact mode with an ionization voltage of 70 eV and data was acquired using MSD ChemStation software (revision D.01.02.SP1, Agilent Technologies). Analytes were identified based on their mass fragmentation patterns by comparison with those of authentic standards using the National Institute of Standards and Technology Mass Spectral Search Program (version D.05.00) ...
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bioRxiv - Immunology 2022Quote: ... Seahorse XF Cell Culture Microplates were pre-treated with poly-D-lysine and 106 primary B cells or 105 DG75 cells were plate in Seahorse XF Base Medium (Agilent) supplemented with 2mM L-glutamine (Agilent) ...
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bioRxiv - Neuroscience 2022Quote: ... Libraries were analyzed with Qubit and fragment size was accessed using the high sensitivity D-5000 screen tapes (5067-5592 Agilent) on a Tapestation.
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bioRxiv - Immunology 2024Quote: ... and MØ cell culture media was replaced with FCS-and bicarbonate-free DMEM medium supplemented with 4.5 mg ml-1 D-glucose and 2 mM glutamine (Agilent, USA) for another 60 min incubation at 37°C without CO2 ...
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bioRxiv - Neuroscience 2023Quote: Anti-PrP monoclonal antibody ICSM35 (D-Gen Ltd) was used with biotinylated polyclonal rabbit anti-mouse immunoglobulin secondary antibodies (Dako; Agilent) and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit ...
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bioRxiv - Developmental Biology 2023Quote: ... 60sal- MUT2 and 60sal-MUT3 were generated from pENTR/D-TOPO-UAS(5x)-60sal(1x) using Pfu polymerase and Quick Change Site-Directed Mutagenesis (Stratagene). Sequences of 6 nucleotides were changed C to A and G to T using the oligonucleotide pairs 60sal- MUT1 Fw and 60sal-MUT1 Rv ...
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bioRxiv - Neuroscience 2023Quote: ... and 5.47*105 cells/cm2 were plated in each well of a poly-D-lysine-coated Seahorse XFe24 V7 PS Cell Culture Microplate (Agilent) or a 24-well tissue culture plate ...
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bioRxiv - Genomics 2023Quote: ... and mock-edited control cells (CTRL) were plated on poly-D-lysine coated 96-well Seahorse assay plates (XF96, Agilent) in DMEM (Gibco A144300 ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 HO-1 +/+ and HEK293 HO-1 -/- were seeded at a density of 2.5 × 104 cells/well on a poly-D-lysine (#P6407-5MG) coated Seahorse XF96/XF Pro cell culture microplate (#204624-100, Agilent) on the day prior to the analysis ...
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bioRxiv - Bioengineering 2024Quote: ... we washed and cultured BMDMs in the Seahorse XF DMEM medium (pH 7.4) supplemented with 25 mM D-glucose and 4 mM Glutamine (all sourced from Agilent Technologies) for an hour in a non-CO2 incubator at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... T140A/D SETD8 cDNAs were generated using the QuikChange Lightning Site-Directed Mutagenesis Kit according to the manufacturer’s instructions (Agilent #210518).
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bioRxiv - Bioengineering 2021Quote: ... samples were subjected to a treatment with 0.3% Sudan black solution for 10 min prior to blocking for 2 h (Dako Blocking Solution X0909; Agilent Technologies, Santa Clara, CA). Immunofluorescence staining was then performed using antibodies to detect CD31 (dilution 1:250 ...
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bioRxiv - Biophysics 2021Quote: Staining of mast cells was done by blocking with serum-free protein followed by incubation for 1 h with anti-tryptase antibody (clone AA1) diluted 1:1,000 in antibody diluent (all Agilent, Santa Clara, CA, USA). For staining of dendritic cells ...
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bioRxiv - Biophysics 2021Quote: ... incubated for 16 h at 4 °C and purified by HPLC on a Zorbax C3 column with dimensions of 21 × 150 mm (Agilent, Santa Clara, CA) using a gradient of 20-90 % acetonitrile in water with 0.1 % trifluoroacetic acid (TFA ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen □ retrieval was performed by incubating the tissue for 2 h at 70 °C in Target Retrieval Solution (Dako, Santa Clara, CA, USA) using a 1:10 dilution in demineralized water ...
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bioRxiv - Microbiology 2024Quote: ... of 0.1 and incubated for 100 h at 30 °C with linear shaking at 567 cycles per minute using an EPOCH microplate reader (Agilent, Santa Clara, CA). The OD600 was measured continuously.
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bioRxiv - Bioengineering 2024Quote: ... samples were treated with 0.3% Sudan black solution for 10 min prior to blocking for 2 h (Dako Blocking Solution X0909; Agilent Technologies, Santa Clara, CA). Immunohistochemical staining was then performed using antibodies to detect laminin (dilution 1:200 ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed three times with TBS-T for 5 min and incubated for 1 h at room temperature with anti-rabbit immunoglobulin/HRP secondary antibody (Agilent, Santa Clara, CA) or with anti-mouse IgG-Peroxidase secondary antibody (MilliporeSigma) ...
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bioRxiv - Cell Biology 2022Quote: ... all cultures were diluted to seed an A600 = 0.01 cells/well into a poly-D-lysine coated Seahorse XFp cell culture miniplate in 50 μl of Seahorse XF assay media (Agilent Technologies). For background measurement ...
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bioRxiv - Physiology 2022Quote: ... The LC-MS/MS data acquired using Agilent Mass Hunter Workstation (.d files) were processed in quantitative analysis software (Agilent Technologies) for quantitative analysis of samples ...
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bioRxiv - Microbiology 2021Quote: ... the fractions were injected on HPLC for purification performed on an Eclipse+ C18 column (L = 150 mm, D = 3.0 mm, Particles diameter 5 µm) (Agilent, Waldbronn, Germany). The volume injected was 100 µl ...
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bioRxiv - Neuroscience 2024Quote: ... d) using a Cascade Summit series semi-automated probe station connected to a 4294A precision impedance analyser (Keysight, Agilent Technologies, model). The measurements were carried out by measuring the total impedance between the counter electrode ...
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bioRxiv - Neuroscience 2023Quote: Anti-PrP monoclonal antibody ICSM35 (D-Gen Ltd) was used with biotinylated polyclonal rabbit anti-mouse immunoglobulin secondary antibodies (Dako; Agilent) and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit ...
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bioRxiv - Cell Biology 2024Quote: ... The glycolytic activity or glycoPER in response to Rotenone/Antimycin A and 2-deoxy-D-glucose was measured using glycolytic rate assay (Agilent, USA). All measurements were performed using Seahorse XFe96 Bioanalyzer (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... oligomycin (1 µM) and 2-deoxy-D-glucose (2-DG) (50 mM) (Seahorse Glycolysis Stress Test Kit, Agilent Technologies, 103020-100). Analyses were performed with Wave software 2.3.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... and fixed with precooled methyl alcohol in −20℃ for 30 min followed by H&E staining (Eosin, Dako CS701, Hematoxylin Dako S3309, bluing buffer CS702), sections were processed at RT by isopropanol (MilliporeSigma ...
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bioRxiv - Microbiology 2020Quote: ... was studied in 50 ml resting cell as mentioned above were monitored till 12 h in an interval of 2 h and later till 48h in an interval of 6 h for determining the rate of CP degradation using high performance liquid chromatography (HPLC) (Agilent Technology 1260 Infinity, USA).
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bioRxiv - Synthetic Biology 2023Quote: ... gain = 75 and mKate (585/615, gain = 125) fluorescence was measured continuously up to 48 h using a Synergy H1 plate reader (Agilent Biotek, Serial Number 21031715) with continuous linear shaking (1096 cpm ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue sections on slides were baked at 60°C for 1 h and then deparaffinized and retrieved on Dako Omnis fully automated IHC platform (Agilent, Santa Clara, CA, USA) using EnVision FLEX target retrieval solution ...
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bioRxiv - Molecular Biology 2024Quote: ... equipped with a refraction index (Surveyor RI Plus and Surveyor) on a 300 × 7.7 mm PL Hi-Plex H+ (8 μm particle size) column (Agilent Technologies, Santa Clara, CA, USA) and 4 × 3 mm ID Carbo-H guard (Phenomenex ...
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bioRxiv - Immunology 2024Quote: ... in 180 µL for 2 h in a nutrient- and serum-limited growth media composed of Seahorse basal DMEM (cat. no. A1443001, Agilent, Santa Clara, CA, USA) supplemented with 0.5 mM D-glucose (555 mM stock ...
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bioRxiv - Cancer Biology 2024Quote: ... the relative cell number per well was measured by replacing the growth medium in each well with fresh growth medium containing 25 µg/ml resazurin sodium salt and incubated for 2 h at 37°C prior to reading fluorescence intensity at 590 nm using a BioTek Synergy HT plate reader (Agilent Technologies, Santa Clara, CA). Wells were then rinsed and refed with growth media containing various drug combinations (Table S2 ...
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bioRxiv - Cell Biology 2022Quote: ... 150,000 cells (activated with anti-CD3 and anti-CD28 antibodies 48 hours prior) were plated into individual wells of a poly-D-lysine coated seahorse cell culture microplate (Agilent; 101085-004). T cell culture medium was changed 1 hour before the start of readout into 180μL serum-free assay medium (Agilent Seahorse XF DMEM ...
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bioRxiv - Immunology 2024Quote: B cells were treated with asparaginase for 18h and 1.5x105 cells were plated on a poly-D- lysine coated 96-well Seahorse utility plate (Agilent, California, United States). Metabolic function was analysed by measuring basal metabolism and metabolism after oligomycin ...
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bioRxiv - Microbiology 2021Quote: ... Concentrations of extracellular metabolites and putative alcoholic contaminants of NAD(P)H substrates were analyzed by high-performance liquid chromatography (HPLC) on an Agilent 1100 HPLC (Agilent Technologies, Santa Clara, CA USA) with an Aminex HPX-87H ion-exchange column (BioRad ...
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bioRxiv - Microbiology 2021Quote: The determination conditions of bacillomycin D and fengycin were established by injecting 10 μL samples into an HPLC column (Eclipse XDB-C18, 5 μm; Agilent, Santa Clara, CA). The temperature was maintained at 30 °C during the experiment ...
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bioRxiv - Immunology 2021Quote: Final V(D)J and 5’ GEX libraries were run on a Bioanalyzer 2100 with a High Sensitivity DNA kit (Agilent, Santa Clara, CA) to assess library size and concentration ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 µg of the plasmid carrying the relevant recombinant provirus was co-transfected with 2 µg of plasmid pMD2.G (a kind gift from D. Trono) and 2 µg of plasmid PVPackgagpol (#217566 from Stratagene, La Jolla, CA) or plasmid pCMV-1′R8.74 (a gift from D ...
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bioRxiv - Bioengineering 2023Quote: ... The optical purity of L-lactic acid and D-lactic acid were measured via a HPLC system (Agilent 1260 series, Hewlett-Packard, USA) equipped with a SCAS Sumichiral OA-5000 column (150 × 4.6 mm ...
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bioRxiv - Molecular Biology 2024Quote: ... in PBST for 1 h at RT Sections were incubated overnight at RT in primary antibody rabbit anti-GFAP (Agilent, Dako Z0334,1:500 in 5 % NGS-PBST) and then with biotinylated secondary antibody anti-rabbit IgG (H+L ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal goat anti-rabbit secondary antibody IgG/HRP (P044801-2; Agilent Technologies/Dako, Santa Clara, 527 CA, United States) diluted 1:2000 in milk ...
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bioRxiv - Molecular Biology 2024Quote: ... in PBST for 1 h at RT Sections were incubated overnight at RT in primary antibody rabbit anti-GFAP (Agilent, Dako Z0334,1:500 in 5 % NGS-PBST) and then with biotinylated secondary antibody anti-rabbit IgG (H+L ...
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bioRxiv - Immunology 2024Quote: ... of the V(D)J libraries was quantified on an Agilent TapeStation 4150 using a D5000 ScreenTape and Reagents (Agilent, 5067-5588, 5067-5589), while the concentration (ng/uL ...
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bioRxiv - Plant Biology 2024Quote: ... The identification and quantification of volatile metabolites was carried out using an Agilent Model 8890 GC and a 7000 D mass spectrometer (Agilent, Palo Alto, CA, USA), equipped with a 30 m × 0.25 mm × 0.25 μm DB-5MS (5% phenyl-polymethylsiloxane ...
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bioRxiv - Genomics 2020Quote: ... Blots were washed three times with TBST for 15min at room temperature and subsequently incubated for 2h at room temperature with HRP-conjugated secondary antibodies (goat anti-rabbit, Dako; rabbit anti-goat, R&D Systems) at a dilution of 1:5000 in TBST ...