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Citations for Agilent :
201 - 250 of 568 citations for 6 nitrobenzo d isoxazol 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were then washed twice with DPBS and mounted using DAKO fluorescent mounting medium containing 4’,6-diamidino-2-phenylindole (DAPI; Agilent). For visualisation of endogenous TDP-43 and FUS ...
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bioRxiv - Cell Biology 2021Quote: ... and were mutated by deleting 6-7 base pairs using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). The primers used for mutagenesis are listed in Supplementary Table S6 ...
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bioRxiv - Pathology 2022Quote: Exonic sequences were enriched in the DNA sample of patient II-6 using SureSelect Human All Exon 50 Mb Kit (Agilent Technologies ...
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bioRxiv - Genetics 2020Quote: ... genomic DNA from various tissues (Supplemental Table 6) was purified and used for library construction with target enrichment using the SureSelectQXT Target Enrichment kit (Agilent). Custom RNA capture probes were designed to hybridize with the 120 bp 5’ ends of the 5’LTRs and the 120 bp 3’ ends of the 3’LTR of about 600 intact (internal region flanked by two LTRs ...
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bioRxiv - Microbiology 2020Quote: ... Small RNAs (6–150 nucleotides) and microRNA fractions (10–40 nucleotides) were quantified using high-resolution small RNA analysis (Agilent 2100 Bioanalyzer system ...
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bioRxiv - Cell Biology 2020Quote: ... The equivalent of 6 ng of resulting cDNA was used as a template for real time PCR using a Mx3000P thermocycler (Agilent) with a home-made SYBR Green qPCR master mix (Lutfalla & Uze ...
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bioRxiv - Neuroscience 2020Quote: Total lysate and synaptoneurosomes isolated from cortex tissue of 1-month-old C57Bl/6 mice were UV crosslinked (100 mJ/cm2 for 2 cycles) using UV Stratalinker 2400 (Stratagene) and stored at −80°C until use ...
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bioRxiv - Cancer Biology 2022Quote: ... For cross validation samples (n=6) the All-In-One solid tumor panel (AIO, Agilent, Santa Clara, USA, catalog # G9706S) was used for capture ...
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bioRxiv - Microbiology 2022Quote: We extracted extracellular metabolites from the seawater matrix using Bond Elut PPL cartridges (1 g/6 ml sized cartridges, Agilent) following the protocol of Dittmar et al ...
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bioRxiv - Biochemistry 2023Quote: ... shRNAs against mouse ACOT12 (#5, #6 and #7) and mouse ACOT8 (#1 and #2) were also constructed in pAdEasy-1 (Stratagene) for adenovirus packaging based on The AdEasyTM Technology (He et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Mitochondrial bioenergetics were assessed at 6 weeks of differentiation using the Mitochondrial Stress Test measured on a Seahorse XFe96 Analyzer (both Agilent). Stress tests were carried out according to manufacturer’s instructions and cycling parameters ...
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bioRxiv - Immunology 2023Quote: For CFTR detection in cross sections, target retrieval was performed with a citrate buffer (Target Retrieval solution, pH 6, (Dako) for 30min after deparaffinization ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA-resistant versions of Cofilin-1 have been obtained by mutating 6 bp of the siRNA-targeting sequence using QuickChange (Agilent). Plasmids were transiently transfected in HeLa cells for 48 hours using X-tremeGENE 9 DNA reagent (Roche) ...
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bioRxiv - Cell Biology 2024Quote: Cell proliferation rates were measured with label-free high-contrast brightfield microscopy in 6-well plates using Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Agilent) as described previously 58 ...
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bioRxiv - Physiology 2024Quote: ... MLO-Y4 cells with Pparγ silenced with siRNA (γY4KD) and their scrambled control (Scrl) were plated 6 hrs before the assay onto Seahorse cell culture microplates (Agilent Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Different FMv2-MDM4 versions containing mutations or deletions of single or combined L22 consensus sequences in MDM4 intron 6 were generated by QuikChange site-directed mutagenesis (200516, Agilent).
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bioRxiv - Microbiology 2023Quote: ... The sample was passed up and down through a 29-gauge needle 6-8 times and the fragment size distribution was determined (∼30 kbp; TapeStation, Agilent).
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bioRxiv - Immunology 2023Quote: ... antigen retrieval using sodium citrate buffer (10 mM, pH 6) was performed for 15 min at 110 °C in a DAKO Pascal pressure chamber (Agilent). After blocking in PBS supplemented with 10% goat serum (Sigma Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were loaded on a Superose 6 Increase column (Cytiva) run by a 1260 Infinity II HPLC (Agilent Technologies) at 0.6 mL/min ...
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bioRxiv - Cell Biology 2020Quote: ... Calu-3 in Seahorse plates were switched to Seahorse XF base media (Agilent 103334-100) supplemented with 10 mM glucose (Fisher BP350) ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was incubated with horseradish peroxidase-conjugated streptavidin (P0397; Dako; 1:3000, 3% BSA) at 4°C overnight ...
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bioRxiv - Pathology 2019Quote: The clinical data and level 3 mRNA microarray data (Agilent 244K TCGA Custom 1-2) of primary GBM samples were downloaded from the NCI Genomic Data Commons ...
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bioRxiv - Neuroscience 2020Quote: ... The slices were washed 3 times with PBS and mounted with Dako mounting medium (Agilent). Fluorescence images of brain section were obtained using a confocal microscope (Zeiss LSM700).
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed 3 times with PBS and mounted using Dako Fluorescent mounting medium (Agilent). Fluorescence images of brain section were obtained using a confocal microscope (Zeiss LSM700).
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bioRxiv - Synthetic Biology 2021Quote: Riboflavin was analyzed using a 50 × 3 mm Poroshell 120 EC-C18 column (Agilent Technologies) in 82% solvent A (20 mM formic acid ...
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bioRxiv - Microbiology 2022Quote: ... Images were acquired by Cytation 3 imaging plate reader (Agilent Technologies, Santa Clara, CA, USA), cell nuclei and LCMV NP expression in infected cells were detected by Gen5 software (Agilent Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... Sections of paraffin-embedded tissue (3 mm) were first treated with Target Retrieval solution (DAKO) and then a cytomation serum-free protein block (DAKO ...
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bioRxiv - Microbiology 2021Quote: ... A bright red chromogen labelling was produced with 3-amino-9-ethylcarbazole substrate (AEC, DAKO). Sections were counterstained with Mayer’s haematoxylin ...
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bioRxiv - Plant Biology 2022Quote: ... camalexin and indole-3-acetonitrile (IAN) were performed using Agilent 1200 HPLC System (Agilent, USA) equipped with diode array and fluorescence (FLD ...
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bioRxiv - Immunology 2023Quote: ... Approximately 3 µg of AC3 was injected into 1290 Infinity II UHPLC system (Agilent Technologies) containing a Premier CSH-C18 column (Waters Corporation) ...
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bioRxiv - Cell Biology 2023Quote: Cellular bioenergetics were measured utilizing the Seahorse XF24-3 Bioanalyzer according to manufacturer’s instructions (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... and heated for 3 minutes (35°-80°C) in a PCR machine (Agilent SureCycler 8800). Next ...
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bioRxiv - Cell Biology 2023Quote: ... and the absorbance was measured at 595 nm with a Cytation 3 Image Reader (Agilent).
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then incubated for 3 h before being analyzed with NovoCyte Flow Cytometer (Agilent). For the other markers ...
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bioRxiv - Neuroscience 2020Quote: Hippocampal neurons were seeded in a poly-d-lysine-coated XF 96-well cell culture microplate (Seahorse Bioscience, Copenhagen, Denmark) in quadruplicate at 5 × 105 cells per well in 1 ml neuronal growth medium and then incubated at 37 °C in 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: Hippocampal neurons were seeded in a poly-d-lysine-coated XF 96-well cell culture microplate (Seahorse Bioscience, Copenhagen, Denmark) in triplicate at 5 × 105 cells per well in 1 ml neuronal growth medium and then incubated at 37 °C in 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a nitrocellulose membrane before probing using a primary anti IL-36γ antibody (Biotechne (R&D) UK) overnight at 4°C and a secondary goat antibody (Dako) for 1 hour at room temperature to visualise any protein cleavage.
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bioRxiv - Plant Biology 2019Quote: ... PAD4LLD was obtained by site-directed mutagenesis on pENTR/D-TOPO PAD4 according to the QuikChangeII site-directed mutagenesis manual (Agilent) (See Table S1 for primers) ...
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bioRxiv - Cancer Biology 2019Quote: ... Immunohistochemistry (IHC) for SEMA4C was performed using a rabbit anti-SEMA4C primary antibody (#AF6125, R & D Systems) on an autostainer (Dako). Detection was achieved using the Envision rabbit detection system (Dako ...
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bioRxiv - Immunology 2019Quote: ... 5×105 CD8+ T-cells were plated onto poly-D-lysine coated wells and assayed in XF RPMI medium (Agilent) pH 7.4 supplemented with 10 mM glucose ...
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bioRxiv - Plant Biology 2020Quote: ... Eluting metabolites were fragmented in electron impact mode with an ionization voltage of 70 eV and data was acquired using MSD ChemStation software (revision D.01.02.SP1, Agilent Technologies). Analytes were identified based on their mass fragmentation patterns by comparison with those of authentic standards using the National Institute of Standards and Technology Mass Spectral Search Program (version D.05.00) ...
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bioRxiv - Immunology 2022Quote: ... Seahorse XF Cell Culture Microplates were pre-treated with poly-D-lysine and 106 primary B cells or 105 DG75 cells were plate in Seahorse XF Base Medium (Agilent) supplemented with 2mM L-glutamine (Agilent) ...
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bioRxiv - Neuroscience 2022Quote: ... Libraries were analyzed with Qubit and fragment size was accessed using the high sensitivity D-5000 screen tapes (5067-5592 Agilent) on a Tapestation.
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bioRxiv - Neuroscience 2023Quote: Anti-PrP monoclonal antibody ICSM35 (D-Gen Ltd) was used with biotinylated polyclonal rabbit anti-mouse immunoglobulin secondary antibodies (Dako; Agilent) and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit ...
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bioRxiv - Developmental Biology 2023Quote: ... 60sal- MUT2 and 60sal-MUT3 were generated from pENTR/D-TOPO-UAS(5x)-60sal(1x) using Pfu polymerase and Quick Change Site-Directed Mutagenesis (Stratagene). Sequences of 6 nucleotides were changed C to A and G to T using the oligonucleotide pairs 60sal- MUT1 Fw and 60sal-MUT1 Rv ...
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bioRxiv - Neuroscience 2023Quote: ... and 5.47*105 cells/cm2 were plated in each well of a poly-D-lysine-coated Seahorse XFe24 V7 PS Cell Culture Microplate (Agilent) or a 24-well tissue culture plate ...
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bioRxiv - Genomics 2023Quote: ... and mock-edited control cells (CTRL) were plated on poly-D-lysine coated 96-well Seahorse assay plates (XF96, Agilent) in DMEM (Gibco A144300 ...
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bioRxiv - Immunology 2024Quote: ... and MØ cell culture media was replaced with FCS-and bicarbonate-free DMEM medium supplemented with 4.5 mg ml-1 D-glucose and 2 mM glutamine (Agilent, USA) for another 60 min incubation at 37°C without CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... For ChR2-mediated optogenetic stimulation, blue light (473 nm, 6 Hz or 20 Hz) was produced using an arbitrary waveform generator (Agilent, 33220A) and a diode-pumped solid-state laser (Laserglow ...