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Citations for Agilent :
2401 - 2450 of 3125 citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... followed by horseradish peroxidase (HRP-) conjugated goat anti-mouse polyclonal antibody (DAKO, Carpinteria, CA, USA) (1:2000 in PBS-Tween 20) ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies used in the study included: anti-WT1 (Dako, ref M3561, clone 6F-H2), anti-MAFB (BETHYL Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... Immunodetection of TTR monomers was performed by employing rabbit anti-human TTR polyclonal antibody (Dako) as a primary and goat anti-rabbit antibody labelled with DyLight 680 (SERACARE ...
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bioRxiv - Neuroscience 2024Quote: ... HRP-conjugated polyclonal antibody (goat anti-rabbit) was purchased from DakoCytomation (Glostrup, Denmark; D048701-2). Mouse LH reference prep (AFP5306A ...
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bioRxiv - Cancer Biology 2024Quote: ... Detection was performed by horseradish peroxidase (HRP)-conjugated secondary antibodies (all from Agilent, CA, USA) and chemiluminescence (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Antibodies were diluted in blocking buffer and sections were mounted in mounting medium (Dako, #S3023).
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bioRxiv - Cell Biology 2020Quote: ... These DN proteins were generated by PCR-based mutagenesis using the QuikChange Lightning Multi Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3.1(+ ...
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bioRxiv - Cell Biology 2019Quote: ... Sections were then treated with Proteinase K (20 μg/mL) for 15 minutes for antigen retrieval and blocked with DAKO background reducing protein blocking agent (Agilent, X090930-2) prior to 1 hour incubation of CD206 (1:200 ...
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bioRxiv - Biophysics 2019Quote: ... Protein concentration was determined using UV absorbance at 280 nm on an Agilent 8453 UV/Vis spectrophotometer (Agilent Technologies, Milford, MA, USA).
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bioRxiv - Biophysics 2021Quote: ... dye at self-quenching concentration of 25 mM and used to monitor increase in fluorescence upon addition of ClyA proteins in a fluorescence spectrophotometer (Agilent Cary Eclipse). To make SUVs at first ...
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with 3% hydrogen peroxide to quench endogenous peroxidase activity and 15 minutes in serum-free protein block (DAKO, Glostrup, Denmark). Sections were then subjected to appropriate antigen retrieval methods (if needed ...
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bioRxiv - Plant Biology 2021Quote: ... at 37⁰C (1:50 protein/trypsin ratio) and cleaned up with a C18 column (OMIX C18, Agilent, Santa Clara, CA, USA). Nano-liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS ...
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bioRxiv - Molecular Biology 2019Quote: The rEDIII protein band was excised from polyacrylamide gel and the sample was prepared for de novo protein sequencing using in-gel digestion according to manufacturer’s protocol (Agilent Technologies, Inc., 2015). Briefly ...
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bioRxiv - Biochemistry 2019Quote: ... IgG was incorporated into sodium dodecyl sulphate polyacrylamide gel and glycans were released from protein using an overnight incubation with PNGase F (ProZyme, Hayward, CA). Released glycans were labelled with 2-aminobenzamide (2-AB ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were washed three times (3X) in TBST and incubated in blocking solution (Protein Block Serum-Free, Agilent DAKO, CA, USA) for 30 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were washed three times (3X) in TBST and incubated in blocking solution (Protein Block Serum-Free, Agilent DAKO, CA, USA) for 30 min at room temperature (RT) ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasma samples were depleted of high- and mid-abundant proteins using immune-depletion columns (Agilent Multiple Affinity Removal Column MARS3 (Agilent 5188-5218) coupled to the Seppro mouse LC5 SuperMix column (Sigma S5824 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated for 1 h at room temperature with rabbit anti-glial fibrillary acidic protein (GFAP, 1:300; Dako, Carpinteria, CA), rabbit anti-oligodendrocyte specific protein (OSP ...
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bioRxiv - Biophysics 2022Quote: Mass spectrometry was performed with protein samples (∼10 μM) with 10% acetonitrile on a 6100 Series Quadrupole LC mass spectrometer (Agilent Technologies, Inc.), equipped with an electrospray source and operated in the positive ion mode ...
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bioRxiv - Biochemistry 2024Quote: ... the GEF protein was added and the absorbance was recorded for an additional on Biotek Synergy HT or Biotek Synergy H1 (Agilent, Winooski, VT). DMSO was employed as vehicle control ...
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bioRxiv - Systems Biology 2022Quote: ... the RNA integrity number was 10 as assessed by the Stanford Protein and Nucleic Acid (PAN) Biotechnology Facility using the RNA Nano Kit (Agilent #5067-1511) on an Agilent Bioanalyzer ...
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bioRxiv - Biophysics 2023Quote: Assessment of the extent of protein oligomerization was based on monitoring absorbance at 550 nm using BioTek Plate Reader (Agilent BioTek NEO2) to reflect turbidity changes induced by protein oligomer formation ...
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bioRxiv - Microbiology 2023Quote: Plasmids encoding hexahistidine (Hisx6)- and glutathione S-transferase (GST)-tagged proteins were introduced into Escherichia coli strain BL21(DE3) CondonPlus RIL (230245, Agilent Technologies, Inc.), and the expression of the recombinant protein was induced by 0.5 mM isopropylthio-β-D-galactoside (IPTG ...
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bioRxiv - Cancer Biology 2020Quote: ... blocked in 0.5% hydrogen peroxide for 5 minutes at room temperature and then blocked with 20% normal rabbit or goat serum (DAKO, Agilent, Cheadle, Cheshire, UK) for 20 min and incubated with the primary antibody overnight at +4°C ...
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bioRxiv - Biophysics 2021Quote: ... Mutations of key contact points between docked ligands and the human 5-HT5AR binding pocket were made via site-directed mutagenesis as directed (Stratagene, La Jolla, CA). Quickchange II mutagenic primer sets were the following ...
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bioRxiv - Microbiology 2021Quote: The determination conditions of bacillomycin D and fengycin were established by injecting 10 μL samples into an HPLC column (Eclipse XDB-C18, 5 μm; Agilent, Santa Clara, CA). The temperature was maintained at 30 °C during the experiment ...
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bioRxiv - Cell Biology 2021Quote: ... was then mutated to either R to mimic deacetylated c-Myc (K323R) or Q to mimic acetylated c-Myc (K323Q) using QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies, 200522-5) against pPHAGE-EF1α-HA-Puro-c-Myc ...
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bioRxiv - Genomics 2020Quote: ... and electroporated in 5 separate cuvettes containing 4 µL of the plasmid and 40 µL of ElectroTen-Blue electrocompetent cells (Agilent Santa Clara, CA). After a 1 hour outgrowth in LB at 37C ...
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bioRxiv - Biophysics 2022Quote: ... 1 and 3.5 μm diameter fibers were manufactured from 2 and 5 wt% of high molecular weight polystyrene (MW: 15,000,000 g/mol, Agilent Technologies, Santa Clara, CA, USA) and equally spaced at approximately 60 μm ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples analyzed by mass spectrometry were resolved using a Poroshell StableBond 300 C8 (2.1 x 75 mm, 5 μm, Agilent Technologies part #660750-906) using a 1290 Infinity II UHPLC (G7120AR ...
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bioRxiv - Biochemistry 2022Quote: ... The pcDNA3.1 Flag-mRBPJ A284V CRr and the pcDNA3.1 Flag-mRBPJ F261A/A284V CRr were generated via site directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies 200521-5) accordingly to manufacturer’s instructions with the oligos listed in Table S6 and using the pcDNA3.1 Flag-mRBPJ WT CRr and the pcDNA3.1 Flag-mRBPJ A284V CRr as templates ...
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bioRxiv - Microbiology 2020Quote: ... The derivatized samples were analyzed on an Agilent GC-MS (GC model 7890A, MS Model 5975C) equipped with a (5% phenyl)-methylpolysiloxane capillary column (Agilent model HP-5MS). The injection port temperature was held at 280 °C and the oven temperature program was held at 80 °C for 1 min ...
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bioRxiv - Biochemistry 2021Quote: ... Analyses were performed on an Agilent Infinity 1260 HPLC system equipped with a 300 × 7.5 mm PLgel SE-HPLC column (particle size 5 μm, pore size 100 Å) (Agilent Technologies, Waldbronn, Germany). Dichloromethane was used as eluent at a flow rate of 1.00 ml/min ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli was carried out with a ZORBAX SB-Aq StableBond Analytical column (5 μm, 4.6 × 250 mm, Agilent Teologies, Santa Clara, CA, USA). A linear gradient of acetonitrile and water with 0.1% trifluoroacetic acid was used for development of the column at a flow rate of 0.8 mL/min ...
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bioRxiv - Genetics 2021Quote: ... Samples were frozen at -80 C until volatiles were separated using a DB-5 column on a Agilent 6890N gas chromatograph attached to an Agilent 5975 mass spectrometer (Agilent, Palo Alto, CA). Volatile quantitation was preformed using ChemStation software (Agilent ...
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bioRxiv - Biochemistry 2021Quote: ... (N-acetyl)paenilamicin-containing fractions were concentrated and purified subsequently by using a Grace HPLC column (GROM-Sil 120 ODS-5-ST, 10 µm, 250 × 20 mm) coupled to an Agilent 1100 HPLC system (Agilent Technologies, Waldbronn, Germany) with a MWD UV detector ...
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bioRxiv - Immunology 2021Quote: Final V(D)J and 5’ GEX libraries were run on a Bioanalyzer 2100 with a High Sensitivity DNA kit (Agilent, Santa Clara, CA) to assess library size and concentration ...
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bioRxiv - Bioengineering 2022Quote: ... and the plates were incubated at 37 °C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 45 hours.
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bioRxiv - Immunology 2022Quote: ... Quality control of the libraries to ensure no adapter dimers were present was carried out by examining 1ul of a 1:5 dilution on a High Sensitivity DNA chip (Agilent Technologies: 5067-4626) on an Agilent 2100 Bioanalyzer ...
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bioRxiv - Plant Biology 2022Quote: ... We analyzed 50 µL of each sample using an Agilent 1100-series HPLC machine with a diode array detector and a Zorbax Eclipse XDB-C18 column (4.6 x 150 mm, pore size 5 µm; Agilent Technologies, Santa Clara, USA). Desulfinated glucosinolates were separated at 40°C in a mobile phase beginning with 1.5% acetonitrile (ACN ...
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bioRxiv - Plant Biology 2022Quote: ... Samples of the final supernatant (5 μL) were automatically injected into a triple quadrupole (QqQ) LC-MS instrument (6430; Agilent Technologies, Waldbronn, Germany) equipped with an Eclipse Plus C18 column (2.1 × 50 mm ...
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bioRxiv - Plant Biology 2023Quote: ... Ten-μl B314-2 sample was injected into an Eclipse XDB-C18 column (250 mm x 4.6 mm, 5 μm, Agilent, Santa Clara, CA, USA) and eluted with the same gradient solvent system described above ...
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bioRxiv - Neuroscience 2024Quote: ... 10ug of total synaptosomal protein was plated in each well of a polyethyleneimine coated 96 well plate (n=5-6/animal) and analyzed using the MitoStress kit from Agilent (Santa Clara, CA). The MitoStress kit measures oxygen consumption rate (OCR ...
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bioRxiv - Immunology 2024Quote: ... using an Agilent 7890B gas chromatograph equipped with a 30 m DB-35ms and 5 m Duraguard capillary column (Agilent, Santa Clara, California) for separation of derivatized metabolites ...
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bioRxiv - Bioengineering 2024Quote: ... and the plate was incubated at 37 °C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 48 hours (Fig ...
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bioRxiv - Bioengineering 2024Quote: ... and the plates were incubated at 37°C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 45 hours.
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bioRxiv - Biochemistry 2023Quote: ... Assays were performed on an Agilent 1100 series HPLC with a reverse phase column (Agilent Zorbax 300SB-C18, 5 μM, 2.1 × 150 mm). Solvent A was water containing 0.1% TFA and solvent B was acetonitrile containing 0.1% TFA ...
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bioRxiv - Microbiology 2023Quote: ... using two different carrier gases (argon and helium) and two stationary phases (CP- Molsieve 5 Å Plot and PoraPLOT U J&W GC columns, Agilent Technologies, Germany). Moreover ...
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). 108 colony-forming units (CFUs ...
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Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... Coverslips were then incubated with 1X DAPI for 5 min before mounting on slides using DAKO mounting media (Agilent Technologies, Cat #S302380-2). Images were captured at 60X or 100X using a Nikon C2+ confocal microscope coupled to NIS-Elements AR software (Nikon).