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Citations for Agilent :
2401 - 2450 of 9205 citations for Mouse Autophagy related protein 16 1 ATG16L1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... For PrP deposition anti-PrP monoclonal antibodies ICSM3525,26 were used in conjunction with biotinylated polyclonal rabbit anti-mouse immunoglobulin secondary antibodies (Dako; Agilent) and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit ...
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bioRxiv - Molecular Biology 2022Quote: ... This was followed by incubation with the appropriate anti-rabbit or anti-mouse IgG antibody conjugated to horseradish peroxidase (Dako) for 60 minutes at room temperature ...
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Treatment with furosemide indirectly increases inhibitory transmission in the developing hippocampusbioRxiv - Neuroscience 2023Quote: ... The membrane was washed 3 times 15 minutes with TBST before a 1h incubation of horseradish peroxidase (HRP)-conjugated antibodies (P0447 goat anti-mouse IgG HRP, Dako, 1:2500 or P0399 swine anti-rabbit IgG HRP ...
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bioRxiv - Biochemistry 2024Quote: ... The presentation of epitopes of the BinJV/WNVKUN when applied to the membrane was confirmed using a goat anti-mouse Ig (Dako) conjugated gold nanoparticle (AuNP ...
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bioRxiv - Neuroscience 2023Quote: Anti-PrP monoclonal antibody ICSM35 (D-Gen Ltd) was used with biotinylated polyclonal rabbit anti-mouse immunoglobulin secondary antibodies (Dako; Agilent) and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit ...
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bioRxiv - Immunology 2023Quote: ... sorted neutrophils were added to CMT-93 (mouse colonic epithelial cells; ATCC) monolayer on 96-well E-plate (Agilent # 300601010), and changes in TEER were measured every 15 minutes for up to 72hrs as described previously (71 ...
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bioRxiv - Biochemistry 2023Quote: The pET-30a-PHB1 vector encoding His-tagged mouse PHB1 was transformed into Escherichia coli strain BL21 Star (DE3; Stratagene). The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare) ...
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bioRxiv - Cancer Biology 2023Quote: ... libraries were constructed and sequenced similarly to the WGS experiment following hybrid capture selection with the hybrid reagent SureSelect DNA - Mouse All Exon V1 (Agilent). WES reads were aligned to the GRCm38 reference genome using BWA-MEM ...
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bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...
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bioRxiv - Cancer Biology 2024Quote: ... Proliferating cells were immunostained with FLEX monoclonal mouse anti-human Ki-67 (Catalog No. GA626, Ready-to-use (Dako Omnis), Clone MIB-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The tissue was washed twice in TBS-T prior incubation with secondary antibody (EnVision anti-mouse-HRP/DAB+ system, Agilent), 3,3′-Diaminobenzidine (DAB ...
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bioRxiv - Biochemistry 2024Quote: ... sections were incubated for 30 min at RT with the detection system (Envision+ System HRP Mouse and Rabbit, respectively; Agilent Dako), followed by incubation with diaminobenzidine as chromogen and counterstaining with hematoxylin.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were washed 3 times with 500 µL PBS before the addition of the secondary antibody (HRP-conjugated anti-mouse IgG (1.5mg/mL) (Dako, Denmark)) diluted 1:2000 in 1% BSA PBS for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound antibody was removed with washing buffer and the fraction of cells with surface protein labeled with CD44 antibody was determined using a MoFlo cell sorter (Dako Cytomation).
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bioRxiv - Molecular Biology 2020Quote: ... All samples were depleted of the 7 most abundant proteins with the multiple affinity removal system (MARS Hu7 spin cartridge) according to the manufacturers protocol (Agilent Technologies). Samples were further reduced using 4 mM DTT ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Primary anti-CD31 antibody in Tris-HCl buffer containing stabilizing protein and 0.015 mol/L sodium azide (Dako Antibody Diluent, Dako, Glostrup, Denmark) were then added to the slides ...
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bioRxiv - Microbiology 2019Quote: ... MBP-fusion proteins were purified with amylose resin following the manufacturer’s instructions (GE Healthcare, Pierce, Agilent Technologies and New England Biolabs). MBP-fusions (1 µg each in 100 µl of 1% NP40 ...
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bioRxiv - Cell Biology 2020Quote: Zyxin and Pxl1 LCR proteins were expressed in Escherichia coli strain BL21-Codon Plus (DE3)-RP (Agilent Technologies, Santa Clara, CA) with 0.5 mM isopropyl β-D-1-thiogalactopyranoside for 16 h at 16 °C ...
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bioRxiv - Immunology 2020Quote: ... followed by a quick rinse in TBS before being returned to the black chamber to be incubated with serum free protein block (Dako X0909) for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of 2 – 4 mg/ml protein samples were applied to a column using the 1260 Infinity HPLC system (Agilent Technologies) coupled to a MiniDawn Treos detector (Wyatt Technologies ...
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bioRxiv - Immunology 2022Quote: ... brucei Heat Shock Protein 70 (TbHSP70) (Kind gift from Professor Keith Matthews, Edinburgh, UK) using a Dako Autostainer Link 48 (Dako, Denmark) and were subsequently counterstained with Gill’s haematoxylin.
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bioRxiv - Biochemistry 2022Quote: ... Proteins were detected using tryptophan fluorescence (λex=280 nm; λem=315 nm) using a fluorescence detector (Agilent technologies 1200 series, G1321A). The peak heights of the monomeric hsFPN peaks were used to assess the stability by normalizing heights to the corresponding value from samples incubated at 4 °C (100% stability) ...
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bioRxiv - Cancer Biology 2020Quote: ... the serum samples were immunoaffinity depleted of the 14 most abundant proteins using IgY14 LC20 (cat No. 5188-6557, Agilent, USA). The depletion was performed on an Agilent 1260 HPLC (1260 Infinity ...
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bioRxiv - Biophysics 2019Quote: ... The intact protein mass was detected by LC/MS on an Open Access MS system (Agilent 1290 UHPLC + Agilent 6530 QToF) and analyzed by the MassHunter software.
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bioRxiv - Biophysics 2019Quote: ... The intact protein mass was detected by LC/MS on an Open Access MS system (Agilent 1290 UHPLC + Agilent 6530 QToF) and analyzed by the MassHunter software.
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bioRxiv - Biochemistry 2021Quote: Serum samples were depleted of the top 14 interfering high-abundant proteins using the Multiple Affinity Removal Column Human 14 (Agilent, USA). Then ...
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bioRxiv - Neuroscience 2024Quote: Free-floating brain sections (8-14 µm thick) were mounted onto slides and blocked with Protein Block (Serum-Free, Dako, CA) for 1h at RT ...
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bioRxiv - Microbiology 2024Quote: ... approximately 2 μg of protein was injected on a Zorbax Poroshell 300SB-C8 column (5 μm, 300Å, 1x75mm IDxL; Agilent Technologies) and separated using a 15 min gradient from 5% to 80% solvent B at a flow rate of 100 μl/min (solvent A ...
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bioRxiv - Cell Biology 2023Quote: ... and the N-terminal 6xHis-tagged and C-terminal EGFP-tagged proteins were expressed in Arctic Express (DE3) RP cells (Agilent Technologies). Bacterial cultures in LB medium were induced with 0.1-0.2 mM IPTG at exponential growth phase (OD600 = 0.5-0.6 ...
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bioRxiv - Molecular Biology 2023Quote: The above 12 OR genes linked to a wild-type internal ribosome entry site from the encephalomyocarditis virus (EMCV IRES) and the mCherry fluorescent protein gene (Shaner et al., 2004) were inserted into a modified pBlueScript KS (Agilent/Stratagene) called plasmid “L” (Zboray et al. ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatants from 1mL transient transfections of the antibodies were purified using the AssayMAP BRAVO platform with 5mL Protein A cartridges (Agilent Technologies). Lager scale transient transfections were purified on the Akta FPLC system using 1mL HiTrap MabSelect PrismA™ affinity chromatography resin (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... Fluorescence spectroscopy measurements of 2 μM of each protein sample were obtained using the Varian Cary Eclipse Fluorescence Spectrophotometer (Agilent Technologies) with an excitation wavelength of 280 nm and recording the emission spectra between 300 nm and 400 nm.
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bioRxiv - Cancer Biology 2023Quote: ... together with 20 μl of pre-washed Protein A/G Dynabeads Chromatin digestion was assessed using TapeStation electrophoresis system (Agilent Technologies). Desired DNA digestion yielded a mono- and poly-nucleosome pattern with a range of DNA fragments 100-1000 bp ...
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bioRxiv - Immunology 2023Quote: ... Antigen retrieval was then performed for 20 minutes at 95°C using Lecia Epitope Retrieval Buffer 2 followed by treatment with Dako serum-free protein block (X090930-2, Agilent Dako) for 15 minutes to prevent non-specific binding of the antibody ...
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bioRxiv - Genomics 2023Quote: Each purified GST-tagged DBD stock was validated for DNA-binding specificity using 4×44k universal protein-binding microarrays (PBMs) (Agilent Technologies), as described previously42 ...
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bioRxiv - Biophysics 2023Quote: ... The monodisperse sample solutions of proteins were injected onto a size exclusion column (David and Perez 2009) (SEC-3, 150 Å; Agilent) using an Agilent HPLC system and eluted into the capillary cell at a flow rate of 0.3 ml min-1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The slides were then treated with 3% hydrogen peroxide for 10 min and then blocked with Serum-Free Protein Block (Dako, X0909) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed thrice with 0.1% (v/v) tween-20 / PBS and incubated in Serum Free Protein Block (DAKO, cat.#X0909) for 1h at 21°C ...
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bioRxiv - Bioengineering 2021Quote: ... The electrical characteristics of the brain phantom were determined with a VNA equipped with a dielectric probe kit (85070E kit, Agilent Technologies, Santa Clara, CA) and were representative of the averaged human brain (er = 66.34 and σ = 0.49 S m−1) ...
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bioRxiv - Neuroscience 2024Quote: ... RNA quality was assessed using an Agilent RNA Nano kit on a BioAnalyzer instrument (RNA 6000 Nano Kit, Agilent, Santa Clara, CA, United States). Libraries were subsequently prepared and sequenced at Psomagen ...
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bioRxiv - Genomics 2024Quote: ... RNA quality was assessed using an Agilent 2100 Bioanalyzer with an RNA 6000 Nano Kit or RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA). ERCC ExFold RNA Spike-In Mixes (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent, cat# Z031101-2 or between 1:4 and 1:5, Dako-Agilent, cat#IR50461-2) followed by 2h at RT with the chicken IgY anti-Neurofilament medium chain (NF-M ...
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bioRxiv - Cancer Biology 2024Quote: ... tissues were incubated with primary antibodies against HER-2 (cell signaling, #4290T, 1:200) or EGFR (cell signaling, #4267, 1:50) and αSMA (Agilent, M085129, 1:100) overnight at +4C ...
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bioRxiv - Biophysics 2020Quote: ... Slides were incubated with primary antibodies against HSV 1 (B0114, Dako; 1:100 dilution) or cleaved caspase 3 (9579 ...
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bioRxiv - Molecular Biology 2021Quote: ... diluted 1:500 in Ab mix or anti-PCNA diluted 1:1000 (DAKO #M0879). The slides were then washed twice in TBS (1% ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies (rabbit anti-AQP4, 1:500, MerckMillipore; rabbit anti-GFAP, 1:500, Agilent Technologies ...
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Integrative analyses of the RNA modification machinery reveal tissue- and cancer-specific signaturesbioRxiv - Molecular Biology 2019Quote: ... 1:150 and 1:2000 respectively with EnVision FLEX Antibody Diluent (K800621, Dako, Agilent) and incubated for 60 min ...
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Integrative analyses of the RNA modification machinery reveal tissue- and cancer-specific signaturesbioRxiv - Molecular Biology 2019Quote: ... 1:150 and 1:2000 respectively with EnVision FLEX Antibody Diluent (K800621, Dako, Agilent) and incubated for 60 min ...
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bioRxiv - Cell Biology 2022Quote: ... followed by a 1-hour incubation with HRP- conjugated secondary antibodies (1:5000, Dako) at RT ...
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bioRxiv - Genomics 2021Quote: ... and examined with the 2100 Bioanalyzer (RNA6000 Pico Kit, Agilent, cat.no ...