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Citations for Agilent :
2301 - 2350 of 4015 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) competent cells were purchased from Agilent technologies (USA) ...
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bioRxiv - Biochemistry 2024Quote: ... the Cell Mito Stress assay kit (Agilent, 103015-100) was used ...
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bioRxiv - Physiology 2019Quote: Tendon fibroblasts were treated for 48 hours prior to being plated (8,000 cells) into collagen coated XFp cell culture miniplates (Seahorse Bioscience, Agilent, Santa Clara, CA). Cultured cells from each treatment condition were reseeded in duplicate ...
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bioRxiv - Cancer Biology 2020Quote: Mel285 and OMM2.5 were seeded in four wells per treatment group at a density of 12,000 and 14,000 cells per well respectively in a 24-well cell culture XFe24 microplate (Agilent Technologies, Santa Clara, CA, USA) at a volume of 100 μl RPMI and allowed adhere at 37°C and 5% CO2 for 5 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... and Cal27 were seeded at a density of 2×104 cells per well in a Seahorse XFe24 Cell Culture Microplate (102340-100; Agilent, Santa Clara, CA). Cells were incubated overnight for adherence in a culture media composed of DMEM and 10% FBS at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... A multichannel pipette was used to transfer 50 µL of the cell suspension (1.5×105 cells) evenly to each well of the PDL plates (Seahorse Bioscience, cat: #103798-100). The cells were centrifuged at 200 rcf (zero braking ...
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bioRxiv - Cancer Biology 2024Quote: The oxygen consumption rate (OCR) of cells was measured using the Seahorse Cell Mito Stress Test kit following the user guide (Agilent, cat. 103010-100). Briefly ...
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bioRxiv - Immunology 2024Quote: ... HER2 CAR-T cells or mock CAR-T cells from the same donor were added at an effector:target cell ratio of 10:1 and measured in a real-time cell analysis assay using the xCELLigence SP system (Agilent, Santa Clara, CA). An electrical current was applied to each co-culture ...
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bioRxiv - Microbiology 2020Quote: ... followed by a 30 min incubation at room temperature with the secondary antibody (Envision mouse and rabbit, respectively, Dako) in an autostainer (Dako) ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed 3x with 0.1% PBST and incubated in an appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (Dako) diluted 1:2000 in 5% milk powder in 0.1% PBST for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were washed three times with PBS containing 0.05% Tween-20 and incubated for 1 h with horseradish peroxidase (HRP)-conjugated secondary antibodies (DAKO) at a dilution of 1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies used were rabbit anti-cow glial fibrillary acidic protein (GFAP; 1:5000; Z0334; Dako, Carpinteria, CA, USA) to detect astrocytes ...
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bioRxiv - Cell Biology 2019Quote: ... Protein lysates were incubated at 4°C with a mouse monoclonal antibody directed against the FLAG-tag (M2, Stratagene). Immunocomplexes were pulled down by incubation with protein G sepharose ...
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bioRxiv - Systems Biology 2019Quote: ... and then incubated at room temperature for 30 minutes with labelled polymer-HRP antibody (Dako EnVision+ System, HRP-DAB). Sections were developed in DAB chromogen solution ...
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bioRxiv - Neuroscience 2019Quote: ... The blots were washed again and an alkaline-phosphate-conjugated goat anti mouse antibody was added (DAKO, 1:2000). Another washing step with TBST and B3 buffer (100 mM Tris ...
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bioRxiv - Bioengineering 2019Quote: N-linked glycans were released from purified antibody samples by overnight incubation with N-Glyccosidase F (Prozyme, GKE-5006B). The proteins were removed using LudgerClean glycan EB-10 cartridges (Ludger ...
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bioRxiv - Neuroscience 2020Quote: ... with 5% BSA and 0.4% triton X-100) followed by incubation overnight at 4°C with 1:500 rabbit anti-C3 antibody (Dako) and 1:10,000 guinea pig anti-vGluT2 (Synaptic Systems ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed with PBS block buffer and antigen-antibody complexes were visualized by incubation with DAB substrate (DAKO) after incubation with Brightvision poly-HRP-linker (Immunologic ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection of primary antibodies (pan-Cytokeratin and α-SMA) was performed by using EnVision-mouse HRP (Dako, Hamburg, Germany) for 45 min at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-E-cadherin (diluted 1:100) antibody (Ab) and anti-vimentin Ab (1:1000) were purchased from DakoCytomation, mouse anti-p65 Ab (1:500 ...
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bioRxiv - Cancer Biology 2019Quote: ... This was followed by sequential 1-hour incubations with the secondary antibodies (En Vision+ System-HRP-Labeled Polymer; Dako) and visualization with the Liquid DAB+ Substrate Chromogen System (Dako) ...
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bioRxiv - Physiology 2021Quote: ... Samples were incubated overnight at 4°C in primary antibodies targeting anti-insulin (1:200, Abcam #Ab7872, Dako #A0564), anti-glucagon (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... Bound monoclonal antibodies were detected with goat anti-mouse immunoglobulin (Ig) conjugated with horse radish peroxidase (HRP, Dako, Denmark). The reaction was developed with TMB one (Kementec Solutions A/S ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were revealed by chemi-luminescence (Super Signal West Dura Substrate, Pierce) using a secondary peroxydase-conjugated antibody (Dako) at a dilution of 1:10000 ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were incubated with a HRP-labeled polymer coupled to a secondary anti-mouse antibody (Flex® system, Dako) for 20 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies and dilutions used were as follows: anti-Mouse Immunoglobulins/FITC (1:400, F0479, Dako, Carpinteria, CA, USA) and anti-Rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2021Quote: ... A representative block of each tumor was sectioned at 4μm thickness and subjected to immunohistochemistry using the following panel of antibodies: CD68 (DAKO, clone PG-M1 ...
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bioRxiv - Cell Biology 2021Quote: Secondary antibodies used were Polyclonal Goat Anti-Rabbit or Polyclonal Rabbit Anti-Mouse (1:1000) HRP-conjugated (Dako Agilent), or IRDye® 680RD (1:20,000)- or 800CW (1:10,000)-conjugated for quantitative fluorescence measurements on an Odyssey® Fc Dual-Mode Imaging System (LICOR Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: Secondary antibodies used were Polyclonal Goat Anti-Rabbit or Polyclonal Rabbit Anti-Mouse (1:1000) HRP-conjugated (Dako Agilent), or IRDye® 680RD (1:20,000)- or 800CW (1:10,000)-conjugated for quantitative fluorescence measurements on an Odyssey® Fc Dual-Mode Imaging System (LICOR Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... then followed by immunoblotting for ≤ 1 h with secondary antibodies: 1:5,000 anti-mouse linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Microbiology 2022Quote: ... then followed by immunoblotting for ≤ 1 h with secondary antibodies: 1:5,000 anti-mouse linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... The secondary antibodies used were goat anti-rabbit and rat anti-rabbit IgG-HRP (Dako, Santa Clara, CA, USA). Proteins were detected by chemiluminescence using the Clarity™ Western ECL Substrate coupled to a ChemiDoc XRS+(Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... and a tyramide-barcode staining cycle performed for mouse anti-MUM1 primary antibody (1:100 dilution, clone MUM1p, Agilent Dako Products ...
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bioRxiv - Cancer Biology 2022Quote: All immunohistochemistry staining were performed at Brigham and Women’s Hospital Pathology Core with validated antibodies against CD68 (clone PG-M1, M0876, Dako), CD3 (clone F7.2.38 ...
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bioRxiv - Immunology 2020Quote: ... followed by the incubation with horseradish peroxidase-labeled secondary antibody (1:10,000) for 1 h at room temperature (DAKO EnVision™+ System ...
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bioRxiv - Neuroscience 2020Quote: Primary antibodies used were listed as follows: rabbit anti-GFAP (a marker for astrocytes, 1:1000, Cat# Z0334, DAKO), rabbit anti-NeuN (a marker for neurons 1:1000 ...
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bioRxiv - Immunology 2020Quote: ... ELISA plates were washed and 50µL of a goat anti-rabbit HRP-conjugated IgG enhancement antibody (EnVision+ System-HRP from Agilent), diluted 1:15 in 2% BSA containing 1% mouse serum to prevent cross-reactivity ...
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bioRxiv - Immunology 2020Quote: ... HRP conjugated secondary antibodies used for Western blotting were anti-rabbit-HRP (0.25 μg/ml, goat polyclonal, Dako, P0448), anti-mouse-HRP (1.3 μg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed with 0.1% Tween-20 in PBS (PBST) and incubated in an appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (Dako) diluted 1:2000 for 2 hours at RT ...
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bioRxiv - Cell Biology 2019Quote: ... washed in PBS before saturation with 0.6 μg/mL mouse IgG2 diluted in Dako Antibody Reducing Background buffer (Dako) for 30 min ...
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Hypusinated eIF5A is expressed in pancreas and spleen of individuals with type 1 and type 2 diabetesbioRxiv - Cell Biology 2019Quote: ... Pancreas tissue sections (8 um) were stained using the following primary antibodies: guinea pig anti-insulin (DAKO; 1:500), goat anti-pancreatic polypeptide (abcam ...
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bioRxiv - Biophysics 2020Quote: ... After rinsing for 5 minutes in PBS they were incubated with secondary antibody (Rabbit Anti-Mouse 1/200, Dako) for 30 minutes ...
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bioRxiv - Pathology 2020Quote: ... It was then incubated with horseradish peroxidase-labelled secondary antibodies (cat. no. sc-2020; 1:5,000; Santa Cruz Biotechnology, Inc.; and cat. no. K4003; 1:5,000; Dako; Agilent Technologies ...
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bioRxiv - Pathology 2020Quote: ... It was then incubated with horseradish peroxidase-labelled secondary antibodies (cat. no. sc-2020; 1:5,000; Santa Cruz Biotechnology, Inc.; and cat. no. K4003; 1:5,000; Dako; Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were subsequently washed three times for 10 minutes in PBS with 0.05% Tween20 and incubated with horseradish peroxidase (HRP) conjugated anti-rabbit or anti-mouse secondary antibody (Dako) in 5% SMP in PBS ...
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bioRxiv - Immunology 2021Quote: ... 10μM ADP or 1μM TRAP-6 in the presence of fluorescein isothiocyanate–conjugated polyclonal rabbit anti-fibrinogen antibody (Agilent Technologies LDA UK Limited ...
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bioRxiv - Microbiology 2021Quote: ... they were then washed three time in PBS containing 0.1% tween 20 and incubated 1h with a rabbit anti-mouse antibody conjugated with horseradish peroxidase (HRP) (Dako). The activity of HRP was revealed by enhanced chemiluminescence (Perkin-Elmer).
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bioRxiv - Cell Biology 2021Quote: VWF string assays were essentially performed as described using 75,000 shCTRL or shSTX5 transduced cells per channel in gelatin-coated 6-channel IBIDI μ-slides VI 0.4.28 Strings were visualized by supplementing perfusion mix with AF488-conjugated anti-VWF antibody (DAKO) at 2 μM concentration ...
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bioRxiv - Neuroscience 2021Quote: ... followed by washing and detection of bound Adu with a rabbit anti-mouse horse radish peroxidase conjugated antibody (Dako) and 3,3′,5,5′-tetramethylbenzidine substrate ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using an anti-GFP antibody (MBL, Code No. 598) and an anti-Rabbit Goat Immuno-globulins/HRP (DAKO, P0160) as 1st and 2nd antibodies ...