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Citations for Agilent :
2251 - 2300 of 2304 citations for Homeobox Protein Hox B1 HOXB1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... followed by repeating all steps from antigen retrieval through TSA using an antibody against CK7 (1:200, mouse; Dako cat. no. MS-1352) and the FITC fluorophore (Perkin Elmer cat ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Primary antibodies were diluted 1:4000 for anti-sGCα1 and 1:2000 for anti-sGCβ1 antibody in 3% dry milk in TBST and incubated with nitrocellulose membranes at 4°C over-night following challenge of membranes with secondary goat anti-rabbit antibody (1:2000 in 3% milk in TBST) conjugated to horseradish peroxidase (Dako A/S, Denmark). Immuno-complexes were visualized using an enhanced chemiluminescence kit (Amersham Pharmacia Biotech ...
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bioRxiv - Pathology 2021Quote: The primary antibodies were incubated at the following concentrations in antibody diluent (PBS + 1% BSA (Fisher BioReagents, BP1605-100) with 0.1% Tween™ 20 (Fisher BioReagents, BP337-500): PanCK (Dako, Z0622, 1:200), Cleaved Caspase 3 (Cell Signaling ...
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bioRxiv - Neuroscience 2022Quote: ... In all rats, antibodies staining for neurons (Mouse anti-NeuN, MAB377, MiliporeSigma) and astrocytes (Rabbit anti-GFAP, Z0334, Dako/Agilent, Santa Clara, CA) were used ...
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bioRxiv - Neuroscience 2022Quote: ... In all rats, antibodies staining for neurons (Mouse anti-NeuN, MAB377, MiliporeSigma) and astrocytes (Rabbit anti-GFAP, Z0334, Dako/Agilent, Santa Clara, CA) were used ...
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bioRxiv - Cancer Biology 2023Quote: ... using the clone DO-7 monoclonal antibody (GA61661-2, Dako Omnis, ready-to-use, incubation 20 min, Agilent Technologies, Santa Clara, CA, USA). Antibody detection was performed using EnVision FLEX/HRP (GV80011-2 ...
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bioRxiv - Neuroscience 2023Quote: ... HLA-DR-and PLP-stained sections were incubated with HRP-labelled anti-mouse antibody (K5007, Dako Real EnVision detection system; Dako, Nowy Sącz, Poland) for 1 hour and immunostaining was visualized with 3’3-diaminobenzidine (1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... The antigen retrieval step was repeated followed by second round staining for pan-CK using anti-pan-cytokeratin antibody (AE1/AE3 mouse monoclonal, Dako-Cat# M351501-2) followed by HRP-conjugated anti-mouse secondary antibody polymer (EnVision+ ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were incubated for 1 hour with horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit antibodies (Agilent Technologies, Santa Clara, CA, USA), washed with PBS 0.05% Tween ...
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bioRxiv - Neuroscience 2023Quote: ... a solution with the secondary antibody and peroxidase (AdvanceTMHRP®, Dako, cat#K4068, Glostrup, Denmark; or EnvisionTM Flex+, Dako, cat#K8002, Glostrup, Denmark) was added for 30 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies bound to proteins were incubated with a secondary antibody labeled with a polymer as part of the Envision + system containing horseradish peroxidase (Dako Cytomation, Gostrup, Denmark). Images of immunohistochemically stained sections were obtained using an optical microscope (BX51 ...
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bioRxiv - Cell Biology 2023Quote: ... before permeabilization with 0.5% Triton X-100 in PBS and stained with primary antibodies: anti-Ki67 (M7240, Agilent Dako, Santa Clara, CA, USA) diluted at 1/50 and anti-Poly-ubiquitin purchased from ENZO (ENZ-ABS840 ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated with the conjugated antibody for one hour before being subjected to flow cytometry analysis on an ACEA NovoCyte Quanteon analyzer (Agilent, Santa Clara, CA). For xenograft TME cellular profiling and in vivo cancer cell HLA expression analysis ...
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bioRxiv - Neuroscience 2020Quote: ... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent, cat# Z031101-2 or between 1:4 and 1:5, Dako-Agilent, cat#IR50461-2) followed by 2h at RT with the chicken IgY anti-Neurofilament medium chain (NF-M ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Slides were then washed and secondary antibody was EnVision+ anti-rabbit labelled polymer-HRP applied for 30min (Dako, K4003; Agilent, Santa Clara, CA, USA), then DAB+ chromogen solution for 5min ...
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bioRxiv - Cell Biology 2020Quote: ... Germany) followed by overnight incubation at 4 °C with specific primary antibodies: polyclonal rabbit anti-human AAT (1:800) (DAKO A/S, Glostrup, Denmark), mouse monoclonal anti-AAT polymer antibody (clone 2C1 ...
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bioRxiv - Microbiology 2020Quote: ... A goat anti-rabbit HRP polymer (Envision +) was used as secondary antibody and color was developed with DAB + (DAKO / Agilent Technologies, Santa Clara, USA).
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bioRxiv - Neuroscience 2019Quote: ... was confirmed by immunostaining with antibody against S100 and were used for measurements of oxygen consumption and extracellular acidification using XF96 extracellular flux analyzer (Seahorse Bioscience, North Billerica, MA) (Yang et al. ...
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bioRxiv - Physiology 2020Quote: ... blocked with 3% BSA in PBS1 and incubated overnight at 4°C with primary polyclonal antibodies for glucagon and insulin (Dako, Agilent, Santa Clara, CA, USA) (Table S8) ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight at 4°C in primary antibody (2% NGS in TBST) with either rabbit anti-GFAP (1:2000, Agilent Cat# Z0334, RRID: AB_10013382), rabbit anti-synaptophysin (IgG ...
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bioRxiv - Neuroscience 2020Quote: ... a monoclonal anti-Aβ17–24 antibody (4G8, Additional file 1, Table S1) was subsequently combined with a polyclonal anti-GFAP (DAKO, Additional file 1, Table S1) as described previously (43) ...
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bioRxiv - Genomics 2021Quote: ... A mouse monoclonal anti-p63 antibody (1:500, MS-1081-P, Neomarkers, Fremont, CA, USA) and followed by a secondary kit (Mouse Envision, DAKO-Agilent, Santa Clara CA, USA), stained with chromogen (3,3′-Diaminobenzidine [DAB] ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Slides were then washed and secondary antibody was EnVision+ anti-rabbit labelled polymer-HRP applied for 30min (Dako, K4003; Agilent, Santa Clara, CA, USA), then DAB+ chromogen solution for 5min ...
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bioRxiv - Cell Biology 2021Quote: ... Dissected aortae were fixed in 4% paraformaldehyde for 30 minutes and then washed with 0.1% Triton X-100 in PBS at room temperature, followed by incubation rabbit anti-β-Catenin antibody (Sigma #C2206, St. Louis, MO in blocking solution (Dako-Agilent, K800621, Carpinteria, CA) (dilution 1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were washed in PBS three times for 10 min after each antibody staining and mounted in DAKO fluorescent mounting medium (Agilent Technologies, cat. no. S3023). The following antibodies were used in this study ...
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bioRxiv - Cell Biology 2023Quote: The following primary antibodies and concentrations were used in this study: rabbit anti-GFAP (DAKO, Agilent, clone Z0334; IF 1:500, WB 1:10,000), mouse anti-GFAP (Sigma ...
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bioRxiv - Immunology 2023Quote: ... blots were washed before adding the secondary antibodies (Polyclonal Swine Anti-Rabbit Immunoglobulins/HRP (Dako p0399; 1:5000 in 1% BSA and TBS-T) and Polyclonal Goat Anti-Mouse Immunoglobulins/HRP (Dako p0447 ...
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bioRxiv - Immunology 2024Quote: ... 2,5.105 PMN were incubated with different treatments and then stained for 30 min with a specific mouse anti-human CD11b antibody conjugated with PE (Dako, Santa Clara, CA, USA) at 4°C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Then cells were fixed in fixed in 4 % of paraformaldehyde in phosphate-buffered saline (PBS) before permeabilization with 0.5% Triton X-100 in PBS and stained with primary antibodies: anti-Ki67 (M7240, Agilent Dako, Santa Clara, CA, USA) diluted at 1/50 and anti-Poly-ubiquitin purchased from ENZO (ENZ-ABS840 ...
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bioRxiv - Immunology 2021Quote: ... a monoclonal mouse antibody against the intracellular tail of human HLA-DR α chain was used (Clone TAL.1B5, # M0746, Agilent, Santa Clara, CA, USA). Detection of MHC-I on the surface of cells was performed using a monoclonal mouse antibody against devil beta-2-microglobulin (B2M ...
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bioRxiv - Molecular Biology 2020Quote: ... monoclonal) (Cell Signalling, Danvers, USA), β-actin (#3700, monoclonal) (Cell Signalling, Danvers, USA), and secondary antibodies (P044801-2, polyclonal) from Dako (Agilent, Santa Clara, USA) were used at a dilution 1/1000 (primary ...
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bioRxiv - Neuroscience 2020Quote: ... the myoblasts were incubated overnight at 4 °C with primary antibodies against sarcomeric myosin, MF-20 (Developmental Studies Hybridoma Bank, Iowa City, IA, 1:200) and Desmin (Dako, Santa Clara, CA, 1:100). After washing with PBS ...
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bioRxiv - Immunology 2022Quote: ... and a tyramide-barcode staining cycle performed for mouse anti-MUM1 primary antibody (1:100 dilution, clone MUM1p, Agilent Dako Products, catalog number GA64461-2). However ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies diluted in blocking solution were incubated overnight at 4°C (anti-GFAP z0334 DAKO 1:400, anti-Iba1 019-19741 Wako 1:400). After three washes in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... for 1 hour at room temperature followed by treatment with HRP-conjugated anti-mouse secondary antibody (DAKO Envision Plus HRP kit, Dako Denmark A/S, Glostrup, Denmark) for 30 minutes ...
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bioRxiv - Physiology 2019Quote: ... tissues were incubated with primary antibody over night at 4°C (guinea pig anti-insulin at 1:750, DAKO; diluted in 1% goat serum/PBS). This was followed by 1-hour incubation with fluorophore-conjugated secondary antibodies (Cy3-anti-guinea pig ...
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bioRxiv - Physiology 2020Quote: ... blocked with 3% BSA in PBS1 and incubated overnight at 4°C with primary polyclonal antibodies for glucagon and insulin (Dako, Agilent, Santa Clara, CA, USA) (Table S8) ...
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bioRxiv - Genomics 2021Quote: ... A mouse monoclonal anti-p63 antibody (1:500, MS-1081-P, Neomarkers, Fremont, CA, USA) and followed by a secondary kit (Mouse Envision, DAKO-Agilent, Santa Clara CA, USA), stained with chromogen (3,3′-Diaminobenzidine [DAB] ...
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bioRxiv - Cell Biology 2021Quote: ... Dissected aortae were fixed in 4% paraformaldehyde for 30 minutes and then washed with 0.1% Triton X-100 in PBS at room temperature, followed by incubation rabbit anti-β-Catenin antibody (Sigma #C2206, St. Louis, MO in blocking solution (Dako-Agilent, K800621, Carpinteria, CA) (dilution 1:1000 ...
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bioRxiv - Immunology 2022Quote: ... then incubated in 5 μg/ml primary antibody in incubation buffer (anti-Mala s 1 mouse monoclonal IgG1 antibody (9G9) from Karolinska Institute (Zagari A et al, 1994, Schmidt et al, 1997) or mouse monoclonal IgG1 antibody (Agilent Dako X093101-2; Santa Clara, USA) as an isotype control for 90 min ...
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bioRxiv - Pathology 2022Quote: ... sections were incubated overnight at 4°C with a primary monoclonal antibody against CYP24A1(sc-365700, Santa Cruz Biotechnology Inc, CA, USA. The sections were then incubated with EnVision (Dako, Agilent, Santa Clara, CA, USA) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... the cells were washed once with the same buffer and incubated with FITC-conjugated polyclonal swine anti-rabbit immunoglobulin antibody from DAKO (dilution 1:40 in BP) for 1 hr at 4°C on ice with shaking ...
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bioRxiv - Microbiology 2022Quote: ... The resulting membrane was blocked for 1 hr in PBS + 5% BSA (Fisher Bioreagents, BP1605-100) before being incubated overnight with primary antibodies [anti-VP1 (Dako, anti-enterovirus clone5-D8/1) or anti-eIF4G (Cell Signaling Technology ...
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bioRxiv - Synthetic Biology 2023Quote: ... Immunodetection was carried out by blocking PVDF membranes in 5% (w/v) fat-free dried milk powder followed by incubation overnight in rat anti-FLAG antibodies diluted 1:500 (Agilent Technologies, Santa Clara, CA, USA). After a brief rinse in Tris-buffered saline with Tween™ 20 detergent (TBST) ...
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bioRxiv - Cancer Biology 2023Quote: ... IHC was performed on an automated Ventana Discovery Ultra staining system using primary antibodies recognizing Ki-67 (M7240, DAKO, mouse monoclonal, clone MIB1, 1:50) for 30min ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated with the required primary antibody (ABri: 338 1:1000 from Ghiso lab; ADan: 5282 1:1000 from Ghiso lab; CD68: 1:150 DAKO; CR3/43: 1:100 DAKO) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Blocking was performed in 0.03% H2O2 containing sodium azide for 5 minutes and primary antibodies (Table S1) were incubated for 60 minutes before detection (Dako EnVision+ System-HRP labeled Polymer) for 30 minutes and development for 5 minutes ...
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bioRxiv - Genomics 2020Quote: ... Blots were washed three times with TBST for 15min at room temperature and subsequently incubated for 2h at room temperature with HRP-conjugated secondary antibodies (goat anti-rabbit, Dako; rabbit anti-goat, R&D Systems) at a dilution of 1:5000 in TBST ...
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bioRxiv - Pathology 2022Quote: ... sections were incubated overnight at 4°C with a primary monoclonal antibody against CYP24A1(sc-365700, Santa Cruz Biotechnology Inc, CA, USA. The sections were then incubated with EnVision (Dako, Agilent, Santa Clara, CA, USA) for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal goat anti-rabbit secondary antibody IgG/HRP (P044801-2; Agilent Technologies/Dako, Santa Clara, 527 CA, United States) diluted 1:2000 in milk ...