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Citations for Agilent :
2251 - 2300 of 2370 citations for 5 4 Iodophenoxy methyl furan 2 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Plasmids encoding AlgE7 variants (Table 2) were constructed based on wild type AlgE7 (plasmid pBG27) (12) using the QuikChange™ Site-directed Mutagenesis Kit (Stratagene/Agilent) or Q5 site directed mutagenesis (New England Biolabs) ...
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bioRxiv - Cell Biology 2021Quote: ... FAMEs were re-dissolved in 2 ml pentane and chromatographed using a GC/MS instrument (SHIMADZU, QP2010 Ultra) with a DB-23 GC column (Agilent, 122-2332). FAME peaks were identified according to the fatty acid standards and integrated to calculate the TAG amount ...
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bioRxiv - Plant Biology 2021Quote: ... Grown colonies were screened with colony PCR using CP-F and UnivR primers and KAPA2G Robust HotStart Kit (Agilent, Supplemental Method 2). Sanger sequencing of the selected clone confirmed correct sequence of the PVY-coding part and correct in-frame insertion of GFP coding sequence ...
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bioRxiv - Immunology 2021Quote: ... Paraffin sections (4µm thick) of FFPE thymus and lymph node tissues (NMR, mouse, and human control) were stained for cytokeratin (AE1/AE3, Dako GA05361-2) on a Dako Omnis autostainer with pressure cooker antigen retrieval (TrisEDTA ...
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bioRxiv - Microbiology 2021Quote: ... were acidified with 5 µl of 30% HCl per 2 mL to prevent precipitation and measured by inductively coupled plasma optical emission spectroscopy (ICP-OES, Agilent Technologies 5100). Porewater for dissolved inorganic carbon (DIC ...
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bioRxiv - Cancer Biology 2020Quote: ... slides with PCa tissue samples (n=144) were incubated at 60°C for 2 hours followed by target retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
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bioRxiv - Cell Biology 2021Quote: ... Entry clones for other mutant dynamin 2 were prepared by introducing corresponding mutations into the Entry clone of wild type human dynamin 2 using QuikChange Lightning Site-directed Mutagenesis kit (Agilent Technologies, 210518) following manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... by polymerase chain reaction (PCR). Ki-67 (Catalog no. M7240) and CD4 (Catalog no. M731029-2) antibodies were purchased from Dako (CA, USA). FOXO3a (Catalog no ...
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bioRxiv - Microbiology 2022Quote: ... Sialyl-Lewis a (sLea) and Sialyl-Lewis x (sLex/CD15s) were detected with 2 µg/ml of mAbs NS-1116-19.9 (Dako, Agilent Technologies, USA) and CSLEX-1 (Becton Dickinson ...
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bioRxiv - Neuroscience 2021Quote: ... the co-cultures were incubated first with primary antibody for glial fibrillary acidic protein (GFAP, 1:400, Z033429-2, Dako, Glostrup, Denmark) prepared in 5% NGS in DPBS overnight at +4 following an incubation with Alexa Fluor405 (A31556 ...
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bioRxiv - Cancer Biology 2021Quote: ... Sections were washed with TBST and slides were developed by adding AEC+ High Sensitivity Substrate Chromogen Ready to use (Dako K346111-2).
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bioRxiv - Immunology 2021Quote: Point substitutions within RBD in SARS-CoV-2 spike gene were introduced by site-directed mutagenesis using the QuikChange II kit (Agilent Technologies Inc.) following the manufacturer’s protocol and by overlapping PCR strategy as described previously (Patil et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... and incubated for 30 minutes at room temperature with the secondary antibody (Supplementary Table 2)(1:500 diluted in DAKO Antibody Diluent). Cells were washed with 0.2% Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... three washes with phosphate buffered saline (PBS) and the incubation with the primary antibody in the DAKO Real TM antibody diluent (Agilent #S202230-2) for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Sialyl-Lewis a and Sialyl-Lewis x (sLex also known as CD15s) were detected with 2 μg/ml of mAbs NS-1116-19.9 (Dako, Agilent Technologies, USA) and CSLEX1 (Becton Dickinson ...
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bioRxiv - Microbiology 2021Quote: ... were designed based on the DNA sequence for SARS-CoV-2 Wuhan-Hu-1 using the QuickChange Primer Design tool (Agilent Technologies, Inc.). Mutagenesis was carried out on a pCDNA-SARs2 Wuhan-Hu 1 S plasmid to create the P681H mutation ...
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bioRxiv - Genomics 2021Quote: ... After 10 minutes of formalin fixation of the tissue on the slides they were dried with isopropanol and stained with Mayer’s hematoxylin (Dako, cat.no.: S330930-2) and bluing buffer (Dako ...
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bioRxiv - Microbiology 2022Quote: ... the plates were incubated with serially diluted serum samples for 2 h at room temperature and antibody binding detected using horseradish peroxidase–labelled rabbit anti-guinea pig antibody (Dako, Glostrup, Denmark) and 5,5’-dithiobis-(2-nitrobenzoic acid) ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were rinsed in PBS (2 × 10 min) before antigen-retrieval by incubation in target retrieval solution (Dako S1700, Glostrup, Denmark) for 40 min at 85 °C ...
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bioRxiv - Immunology 2023Quote: ... following standard protocol and plated at a concentration of 2×105 cells per well of a seahorse XFe96 assay plate in seahorse XF DMEM media (Agilent; 103575-100) supplemented with 1mM pyruvate ...
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bioRxiv - Cancer Biology 2023Quote: FISH assay was performed on tissue microarrays using the Histology FISH accessory kit (K579911-2, Dako, Agilent Technologies, Santa Clara, CA, USA) according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor xenograft slides were incubated at 60 °C for 2 h followed by antigen retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
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bioRxiv - Bioengineering 2022Quote: ... Incubation with secondary antibodies diluted in blocking solution was performed 1 h at RT: anti-rabbit-IgG conjugated to horseradish peroxidase (1:10,000, cat. no. P044801-2; Dako, Carpinteria, CA, USA), anti-mouse-IgG conjugated to horseradish peroxidase (1:10,000 ...
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bioRxiv - Biophysics 2022Quote: Point substitutions within RBD in SARS-CoV-2 spike gene were introduced by site-directed mutagenesis using the QuikChange II kit (Agilent Technologies Inc.) following the manufacturer’s protocol and by overlapping PCR strategy as described previously (33) ...
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bioRxiv - Neuroscience 2023Quote: ... samples were centrifuged at 20.800 xg for 2 min and 100 µl sample was injected on a SEC-HPLC column (Bio SEC-3 300 Å, Agilent, USA) using an Agilent 1260 Infinity II system (Agilent ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A 2 µl aliquot of processed gDNA was taken to assess shearing using an Agilent High Sensitivity D1000 Kit (Agilent, Cat. #G2991AA). Once shearing was assessed ...
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bioRxiv - Microbiology 2023Quote: ... 200 mm by 2 mm fitted with a precolumn 10 mm by 2 mm (Dr. Maisch GmbH, Ammerbuch, Germany) coupled to an Agilent LC/MSD Ultra Trap System XCT 6330 (Agilent, Waldbronn, Germany). For analysis were used ...
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bioRxiv - Microbiology 2024Quote: ... 1:2 dilution series in serum-free media was performed and cells were infected in a 96-well plate (Agilent, 204624-100) for 1 hour at 37°C followed by addition of methylcellulose (Sigma M0512-500G ...
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bioRxiv - Neuroscience 2024Quote: ... Wildtype mouse brain paraffin sections were used for IHC analysis with a dilution of mouse serum at 1:200 with antibody diluent solution (DAKO, S080983-2). Mouse anti-NMDAR1 monoclonal antibody (BD ...
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bioRxiv - Microbiology 2024Quote: ... The optical density (OD) at 600 nm was recorded every hour using a BioTek Epoch 2 Microplate Spectrophotometer (Agilent Technologies, CA, USA) using Gen 6 1.04 software (Agilent Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed in PBS and incubated 30 min in an anti-rabbit polymer system (Anti-rabbit Envision HRP polymer system, Agilent K400311-2). After several rinses ...
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bioRxiv - Microbiology 2024Quote: ... Clarified supernatant (2 µl) was dotted onto nitrocellulose as above and probed with rabbit polyclonal anti-CEA primary antibody (1:1000; Dako, Denmark A0115) and HRP conjugated anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... at 450 nm was measured 2 hours after the addition of the CCK-8 reagent using BioTek Synergy H1 microplate reader (Agilent Technologies, USA).
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bioRxiv - Cell Biology 2024Quote: qPCR was performed using 2× SYBR Green qPCR Mix (Low ROX) (Aidlab Biotechnologies, Beijing, Chian) in a Stratagene Mx3000P (Agilent Technologies, SUA). With GAPDH mRNA as endogenous control ...
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bioRxiv - Immunology 2024Quote: ... Stimulated BMDMs were washed with XF media (non-buffered RPMI-1640 containing 2 mM L-glutamine and 1 mM sodium pyruvate; Agilent Seahorse XF24) and incubated for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... on a Dako Omnis platform for 1 h and secondary antibody for 30 minutes followed by substrate chromogen (DAB) (DAKO, GV82511-2) treatment for 10 minutes and counterstaining with haematoxylin ...
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bioRxiv - Cell Biology 2024Quote: ... on a Dako Omnis platform for 1 h and secondary antibody for 30 minutes followed by substrate chromogen (DAB) (DAKO, GV82511-2) treatment for 10 minutes and counterstaining with haematoxylin ...
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bioRxiv - Synthetic Biology 2020Quote: ... Fractions containing the target peaks were combined and further purified by a Reveleris flash chromatography system (Grace) using a C18 Preparative column (Agilent, 5 μm, 21.2 × 150 mm). A gradient method (55% acetonitrile-water to 85% acetonitrile-water in 12 mins ...
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bioRxiv - Biochemistry 2020Quote: ... were separated by a gradient elution from 5% solvent B to 95% solvent B over 15 min on a high-capacity nano-LC chip (Agilent Technologies; part no. G4240-62010) driven by a 1200 series nano-flow HPLC system (Agilent ...
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bioRxiv - Synthetic Biology 2022Quote: ... Further purification of the target product was performed using a reverse-phase HPLC semi-preparative column (Agilent Zorbax SB C18, 250 × 9.4 mm, 5 μm) to collect the target compound manually ...
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bioRxiv - Microbiology 2022Quote: ... The resulting membrane was blocked for 1 hr in PBS + 5% BSA (Fisher Bioreagents, BP1605-100) before being incubated overnight with primary antibodies [anti-VP1 (Dako, anti-enterovirus clone5-D8/1) or anti-eIF4G (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2024Quote: ... and the molar mass distribution in PVC materials were determined using a 1260 HPLC system equipped with three PLgel 5 µm Mixed-C columns and a PLgel guard column (Agilent Technologies, Santa Clara, CA, USA). Approximately 5 mg of PVC was dissolved in 1 mL of tetrahydrofuran at room temperature for at least 14 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Blocking was performed in 0.03% H2O2 containing sodium azide for 5 minutes and primary antibodies (Table S1) were incubated for 60 minutes before detection (Dako EnVision+ System-HRP labeled Polymer) for 30 minutes and development for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The cytolysis of A549 cells was monitored over a period of 5 days using xCELLigence® RTCA eSight device (Agilent Technologies, Inc., Santa Clara, CA) by impedance.
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bioRxiv - Molecular Biology 2023Quote: ... Liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) was performed using a C3 column (Agilent Poroshell 300SB-C3, 1 x 75 mm, 5 µm) with a gradient of 5% to 95% acetonitrile (0.1% v/v formic acid ...
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bioRxiv - Microbiology 2024Quote: ... Mutation of the catalytic active site at position 138 (Ser-138 to Ala-138) of RLip was introduced via the QuikChange II XL site-directed mutagenesis kit (Agilent technologies, Cat-No.: 200521-5) according to the manufacturer’s instructions using the using Forward primer 5’-CTTTTAACACCAGCGGCCACGCGCTGGGCGCGATTATC-3’ and Reverse primer 5’-GATAATCGCGCCCAGCGCGTGGCCGCTGGTGTTAAAAG-3’ ...
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bioRxiv - Cancer Biology 2024Quote: ... 2× 104 EO771 and PyMT cells were cultured in 100 µL of DMEM supplemented with 10% FBS and 4 mM glutamine with or without S100A8 or S100A8/9 recombinant protein (5 or 10 µg/mL) and plated in XFe96/XF Pro PDL Cell Culture Microplates (Agilent Technologies, Cat. #103799-100, US). Following 16h overnight incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... fixed tissues were embedded in paraffin and 4 mm sections were histologically processed for hematoxylin-eosin staining and immunohistochemistry using anti-human Ki67 antibody (DAKO #M7240, 1:75, 30 min at 37°C). All these procedures were performed using standardized protocols by Atrys Health S.A.
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bioRxiv - Cancer Biology 2021Quote: ... the membranes were washed 3X with TBST followed by incubation with HRP-conjugated secondary antibodies (Rabbit Cytiva/Amersham NA934; Mouse Agilent/Dako P026002-2) for one hour at room temperature ...
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bioRxiv - Genetics 2020Quote: ... and 0.5 mL was pipetted into individual 2 mL glass vials fitted with 9mm PTFE lined caps (Agilent Crosslab, Santa Clara, CA, USA). The hexane was evaporated under a nitrogen gas flow ...