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Citations for Agilent :
2201 - 2250 of 2281 citations for Mono 2 Ethyl 5 Oxohexyl Phthalate 13C4 99% Dehp Metabolite Vi 100Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Local assembly of long reads enables phylogenomics of transposable elements in a polyploid cell linebioRxiv - Genomics 2022Quote: ... Purified DNA was precipitated by addition of 8 mL of ethanol and resuspended in TE buffer and size was analyzed by TapeStation (Agilent) prior to library preparation ...
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bioRxiv - Molecular Biology 2020Quote: ... Sensor plates were calibrated overnight in a CO2-free incubator at 37°C using 1 mL/well of XF calibrant solution (Agilent). After polarization ...
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bioRxiv - Microbiology 2021Quote: ... Nucleus staining was performed with DAPI 1 µg/mL for 1 min and coverslips were mounted on slides with fluorescent mounting medium (Dako). Cells were observed under a SP8 confocal laser-scanning microscope equipped with an HCP PL APO 100x/1.44 Oil CORR CS immersion objective (Leica) ...
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bioRxiv - Plant Biology 2019Quote: ... β-caryophyllene and 20 mg indole (Erb et al., 2015) was added into separate 2.0 ml amber glass vial (Agilent Technologies) with 1 mg of glass wool (Fig ...
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bioRxiv - Cancer Biology 2021Quote: ... SPEC-PTSCX sample cleanup pipette tips and Bond Elut C18/ 3 mL cleanup cartridges were from Agilent (Santa Clara, CA), cell culture slides (8-chamber ...
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bioRxiv - Cancer Biology 2020Quote: ... sections were blocked in DAB substrate-chromogen solution containing 1 ml substrate buffer and 1 drop of DAB chromogen (DAKO) for 10 minutes followed by rising with dH2O for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... five grams were sampled and placed in a vial (HS, crimb, FB, 20 ml, clr, cert, 100 PK, Agilent Technologies) hermetically capped by a pressure plug with a plastic membrane ...
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bioRxiv - Biophysics 2022Quote: TTP was run on a tandem size exclusion chromatography - multi-angle light scattering detector by injecting 100 μL sample at a concentration of 1 mg/mL using a 1260 Infinity HPLC (Agilent). The column was pre-equilibrated in 0.1 μm filtered buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... and probed with 8ng/mL anti-calnexin antibody (Cat. No. 2433S, Cell Signalling) overnight followed by 25ng/mL goat anti-rabbit -HRP (Cat. No. P0448, Agilent) secondary antibody or with 600ng/mL anti-GAPDH directly conjugated with HRP (Cat ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... OCI-AML3 and NALM6 cells stained with PBS containing 0.1 μg/mL acridine orange were evaluated for GFP and RFP channels using a fluorescent microscope (Lionheart FX Automated microscope; Agilent BioTek Instruments Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with 1 μg/mL recombinant anti-PSD95 antibody (Rabbit Fc fusion, NanoTag Biotechnologies #N3783) diluted in Dako REAL Antibody Diluent (S2022, Agilent) for 1 h at RT ...
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bioRxiv - Plant Biology 2022Quote: ... The digested solution was diluted to 15 mL with ultrapure water and analyzed by ICP-OES (Vista-PRO CCD Simultaneous ICP-OES; Agilent) calibrated with standards ...
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bioRxiv - Neuroscience 2023Quote: ... The steroid extraction was performed as earlier described for plasma [39] with a further modification of the CSF analysis to encompass a different size of solid-phase extraction columns (100 mg Bond elute C18 solid-phase extraction cartridges; 1 ml; Agilent) and therefore corresponding volume changes for conditioning (1 ml MeOH followed by 2×1 ml dH2O) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... to assess levels of serum psilocin and 2C-B (ng/mL) using liquid chromatography-tandem mass spectrometry liquid chromatography-tandem mass spectrometry (LC-MS/MS; Agilent, Waldbronn ...
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bioRxiv - Biochemistry 2022Quote: ... was injected at a concentration of 10mg/ml onto a Superdex S75 (10/300, Cytiva) mounted on a high-pressure liquid chromatography system (1260 Infinity; Agilent). Species were separated using 25 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... Shimadzu LC-10AT; autosampler: HiP sampler G1367A, T = 4°C, 10 µL injection; flow rate: 1 mL/min; column: Agilent Zorbax Eclipse XDB-C18 80Å ...
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bioRxiv - Systems Biology 2023Quote: ... with a split flow of 10 mL/min into a DB-5ms 40 m× 250 µm × 0.25 µm GC column (Agilent Technologies). We used helium as the carrier gas at a flow rate of 1 mL/min and set the inlet to 250 ◦C and programmed the GC oven to 60 ◦C for 1 min ...
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bioRxiv - Immunology 2023Quote: ... 10 µg/mL of either mouse anti-human LAIR-1 (clone Dx26; purified in house) or mouse isotype control IgG1 (Dako) diluted in PBS+1% BSA buffer were incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Concentrated protein samples were then immunodepleted using a multi-affinity removal spin cartridge human-14 (0.45 mL, Agilent Technologies, UK) to remove high abundance proteins according to the manufacturer’s protocol with some modifications ...
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bioRxiv - Cell Biology 2024Quote: ... One aliquot was solubilized with 1% SDS to determine protein content and the other aliquot was mixed with 1 mL 30% HNO3 and subjected to ICP/MS 8900 (Agilent). Data was normalized by the corresponding protein concentration.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were washed 3 times with 500 µL PBS before the addition of the secondary antibody (HRP-conjugated anti-mouse IgG (1.5mg/mL) (Dako, Denmark)) diluted 1:2000 in 1% BSA PBS for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... then incubated in 5 μg/ml primary antibody in incubation buffer (anti-Mala s 1 mouse monoclonal IgG1 antibody (9G9) from Karolinska Institute (Zagari A et al, 1994, Schmidt et al, 1997) or mouse monoclonal IgG1 antibody (Agilent Dako X093101-2; Santa Clara, USA) as an isotype control for 90 min ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were washed once with DMEM Assay Medium (XF DMEM [Agilent] supplemented with XF glucose [10 mM, Agilent], XF glutamine [2 mM, Agilent] and XF pyruvate [1 mM, Agilent]), then left in DMEM Assay Medium and placed in a non-CO2 incubator for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal goat anti-rabbit secondary antibody IgG/HRP (P044801-2; Agilent Technologies/Dako, Santa Clara, 527 CA, United States) diluted 1:2000 in milk ...
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bioRxiv - Biochemistry 2023Quote: The crude peptide mixture was purified using reverse-phase high performance liquid chromatography (RP-HPLC) on a semi-preparatory C18 column (Agilent, ZORBAX 300SB-C18, 9.4 x 250 mm, 5 μm) with an Agilent HPLC system (1260 Infinity II) ...
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bioRxiv - Bioengineering 2024Quote: ... samples were collected immediately after euthanizing the animals and subjected to radio-HPLC (Agilent 1260 Infinity II HPLC with Agilent column: 300SB-C18, 9.4 X 250 mm, 5 µm). The mobile phase started with 95% solvent A (0.1% TFA in water ...
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bioRxiv - Cancer Biology 2021Quote: ... In vivo psoralen cross-linking of the DNA was achieved by a repetitive exposure of living cells to 4,5’,8-trimethylpsoralen (10 μg/ml final concentration) followed by irradiation pulses with UV 365-nm monochromatic light (UV Stratalinker 1800; Agilent Technologies). The cells were then lysed with cell lysis buffer (buffer C1 ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were treated with AEVs from AD and normal participants for 1 hour and then subjected to fluorescent immunolabelling for MAC using a mouse anti-human C5b-9 antibody (1 μg/mL; RRID: AB_2067162, cat. no. M0777, Dako, Carpinteria, CA) targeting an epitope in the poly-C9 component of MAC in association with C5b ...
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bioRxiv - Biochemistry 2021Quote: ... Nbs were mixed with hArc-CTD in 1.3-fold molar excess for a final complex concentration of 10 mg/mL and 50 μl of the mixture injected onto an AdvanceBio SEC 130 column (Agilent Technologies) pre-equilibrated with the running buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The combined organic extract was concentrated to ~1 mL prior to clean-up by SPE (Agilent Bond Elut SI, 500 mg). The SPE columns were conditioned with hexane and the extract eluted with 6 mL 10% dichloromethane in hexane ...
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bioRxiv - Molecular Biology 2021Quote: ... Antigen concentration was determined using a PLRP-S column (2.1 x 150 mm, 300Å, 3µm) operated at 0.6 mL/min and 80°C (Agilent Technologies, Santa Clara, CA) on an HPLC equipped with a diode array detector set for absorbance detection at 214nm and ...
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bioRxiv - Biochemistry 2020Quote: ... at a flow rate of 0.2 mL/min.and the elution was introduced online into the Q-TOF mass spectrometer (Agilent Technologies, SantaClara, CA) using electrospray ionization ...
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bioRxiv - Physiology 2021Quote: ... fully differentiated myotubes were treated for 24h with 10 ng/ml rNRTN before being subjected to extracellular flux analysis (XF24, Seahorse Bioscience). The mitochondrial stress test assay was performed with 1 mM pyruvate and 5 mM glucose ...
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bioRxiv - Bioengineering 2020Quote: ... Antigen concentration was determined using a PLRP-S column (2.1 x 150 mm, 300Å, 3μm) operated at 0.5 mL/min and 60°C (Agilent Technologies, Santa Clara, CA). Buffer A was 0.1% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... or PDK1SKD-PIF S241A (4 mg/ml) was injected onto a Superdex 200 10/300 column (Cytiva) operated by a 1260 Infinity HPLC (Agilent Technologies). Light scattering of a 690 nm laser was detected by a MiniDawn Treos (Wyatt ...
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bioRxiv - Cell Biology 2024Quote: ... Blocking was done in Primary Blocking Buffer (10% donkey serum, 0.1% saponin, 0.05 mg/mL sonicated salmon sperm DNA (Stratagene, La Jolla, California) in PBS for 1 hour at room temperature ...
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bioRxiv - Pathology 2023Quote: ... EGTA 1 mM and 1mg/ml bovine serum albumin) supplemented with respiratory substrates (glutamate/malate) and 50 nM MitoXpress (Agilent, USA). Each well was loaded with 10μl respiratory substrates ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended at a concentration of 8×106 cells/ml in Seahorse XF Base Medium (Agilent Technologies, Santa Clara, CA) supplemented with 1 mM pyruvate ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was cross-linked by exposing the living cells twice to 4,5′,8-trimethylpsoralen at a final concentration of 10 μg/mL followed by 3’ irradiation pulses with UV 365-nm monochromatic light (UV Stratalinker 1800, Agilent Technologies). The cells were then washed repeatedly with cold PBS and lysed with a cell lysis buffer (1.28M sucrose ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 10 μL of ethanol standard or unknown whole blood was added to 10-mL glass headspace vials (Agilent, Santa Clara, CA) and sealed with a crimp cap ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were washed in TBS-t and subsequently incubated with goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody (0.5 µg/ml; Dako, VIC, Australia) or rabbit anti-Goat IgG horseradish peroxidase-conjugated secondary antibody (0.125 µg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... A glucose-free assay medium was prepared by mixing 10 ml of Seahorse XF base medium minimal DMEM (Agilent 103334-100), 100 µl Na pyruvate (Sigma S8636) ...
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bioRxiv - Genetics 2024Quote: The purified RNAs in the 96-well PCR plate (Axygen Scientific, PCR-96-FS-C) were transferred to a 0.3 mL 96-well plate (Agilent, 5043-9313) for hydrolysis in a 40 µL of an enzyme cocktail containing 10 U benzonase (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with DAPI (1 μg/ml) and mounted onto glass slides using DAKO Fluorescent Mounting Medium (Agilent Technologies S3023). Images were acquired using an Upright ZEISS AXIO Imager.M2 with Apotome 2.
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bioRxiv - Plant Biology 2024Quote: ... Powdered samples (1 g) were weighed and transferred immediately to a 20 mL head-space vial (Agilent, Palo Alto, CA, USA), containing NaCl saturated solution to inhibit potential enzyme reactions ...
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bioRxiv - Plant Biology 2024Quote: ... The samples were homogenized and the supernatant was loaded onto a Bond Elut C18 cartridge (100 mg, 3 ml; Agilent Technologies) and eluted with 80% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Supernatants were loaded into pre-conditioned Phenomenex Strata XL-100 60 mg/3 ml cartridges (Torrance, CA) and passed through it using positive pressure manifold (Agilent Technologies). Flow through was collected subsequently and 100 µL of filtrate was mixed with 100 µL of water for the LC-MS/MS system ...
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bioRxiv - Biochemistry 2024Quote: ... A reference mass correction solution was continually infused at a flow rate of 0.5 mL/min via an external isocratic pump (Agilent, Cheadle, UK) for constant mass correction (see preparation of reference mass correction solution) ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred to amber glass LC vial with 0.250 mL deactivated glass insert and caps with PTFE/ silicone septa (Agilent Technologies Inc.). The pooled quality control (QC ...
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bioRxiv - Plant Biology 2024Quote: ... Fatty acid methyl esters (1 ml) were injected onto a DB-23 capillary column (60 m · 0.25 mm · 0.25 mm (Agilent Technologies, United States). A mix of FAME standards was used as references for peak identification by retention times ...