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Citations for Agilent :
2151 - 2200 of 2659 citations for Human Serum Albumin Rabbit Polyclonal affinity purified since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of the library was hybridized to the Agilent SureSelect XT Human All Exon V4 probes (Agilent Technologies 5190-4634). The targeted exon fragments were captured by Streptavidin beads (Fisher 65602) ...
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bioRxiv - Biophysics 2024Quote: The recombinant human Cα subunit of cAMP-dependent protein kinase with the Phe to Ala mutation in position 100 (PKA-CF100A) was generated from the human PKA-Cα wild-type using Quik-Change Lightning mutagenesis kit (Agilent genomics). The key resource table lists the PCR primers used to modify the pET-28a expression vector encoding for the wild-type human PKA-Cα gene (PRKACA – uniprot P17612 ...
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bioRxiv - Immunology 2020Quote: ... followed by a quick rinse in TBS before being returned to the black chamber to be incubated with serum free protein block (Dako X0909) for 20 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... the serum samples were immunoaffinity depleted of the 14 most abundant proteins using IgY14 LC20 (cat No. 5188-6557, Agilent, USA). The depletion was performed on an Agilent 1260 HPLC (1260 Infinity ...
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bioRxiv - Immunology 2023Quote: ... Antigen retrieval was then performed for 20 minutes at 95°C using Lecia Epitope Retrieval Buffer 2 followed by treatment with Dako serum-free protein block (X090930-2, Agilent Dako) for 15 minutes to prevent non-specific binding of the antibody ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The slides were then treated with 3% hydrogen peroxide for 10 min and then blocked with Serum-Free Protein Block (Dako, X0909) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed thrice with 0.1% (v/v) tween-20 / PBS and incubated in Serum Free Protein Block (DAKO, cat.#X0909) for 1h at 21°C ...
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bioRxiv - Neuroscience 2024Quote: Free-floating brain sections (8-14 µm thick) were mounted onto slides and blocked with Protein Block (Serum-Free, Dako, CA) for 1h at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Blocking was done in Primary Blocking Buffer (10% donkey serum, 0.1% saponin, 0.05 mg/mL sonicated salmon sperm DNA (Stratagene, La Jolla, California) in PBS for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
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bioRxiv - Cancer Biology 2021Quote: ... The reaction mix was purified by lithium chloride precipitation and concentration and size of the transcripts were measured using TapeStation 4200 (Agilent).
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bioRxiv - Cell Biology 2020Quote: ... MBP-Tlg21-327 and the co-expressed Vps45–Tlg2 proteins were expressed and purified in a similar manner with the following modifications: protein was overexpressed in BL21-Codon Plus (Agilent); after IPTG addition the cells were grown at 16°C ...
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bioRxiv - Biophysics 2020Quote: ... and the MFS0 peptide biotin-N-GGGGS (GPGGA)5 GGKYG GS-K(ε-N3) were purified via preparative C18 reverse phase HPLC (5 µm particle size, 250×21.2 mm, Agilent) applying a linear gradient of 0.1% triflouracetic acid (TFA ...
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bioRxiv - Molecular Biology 2021Quote: ... after which quantification and qualification of purified band(s) were undertaken using the Agilent DNA 1000 kit and BioAnalyzer (Agilent). Illumina NGS was performed with 2×250 bp sequencing without fragmenting the amplicons ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl of each purified final library was run on an Agilent TapeStation HS D1000 ScreenTape (Agilent Technologies, 5067-5584). The libraries were quantified using the Quant-iT 1X dsDNA HS kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... The pool was then purified again with 0.65X volumes of AMPure XP Beads and analyzed on a 2200 TapeStation (Agilent Technologies) using a High Sensitivity D1000 ScreenTape (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions showing absorbance peaks at 280 nm were pooled and purified by size exclusion chromatography (SEC) in a HPLC system (Agilent technologies ...
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bioRxiv - Genomics 2021Quote: ... 4 μl of the purified product were amplified in multiple 100 μl reactions using Herculase II Fusion DNA Polymerase (Agilent) following the manufacturer’s specifications with 0.3 μM of the IS5/IS6 primers ...
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bioRxiv - Cancer Biology 2022Quote: ... All uniquely labelled wells from each plate were pooled and multiplexed libraries were purified and quality controlled using TapeStation (Agilent) and Qubit fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... RNA from cultured and transfected DRG cells was purified using the Absolutely RNA Nanoprep Kit (Agilent Tech., Santa Clara, CA). RNA quantity was measured using a NanoDrop 1000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated and purified using the Absolutely RNA Miniprep Kit (Cat# 400800, Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s protocol for extremely small samples ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were purified on C18 stage tips using protocol described previously58 prior to intact mass analyses using LC-TOF mass spectrometer (Agilent).
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bioRxiv - Genetics 2020Quote: ... genomic DNA from various tissues (Supplemental Table 6) was purified and used for library construction with target enrichment using the SureSelectQXT Target Enrichment kit (Agilent). Custom RNA capture probes were designed to hybridize with the 120 bp 5’ ends of the 5’LTRs and the 120 bp 3’ ends of the 3’LTR of about 600 intact (internal region flanked by two LTRs ...
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bioRxiv - Genomics 2021Quote: ... Between 1 and 5 ng of the purified digested DNA was loaded on a High Sensitivity DNA chip (Agilent Technologies) and run on an Agilent 2100 Bioanalyzer System ...
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bioRxiv - Molecular Biology 2020Quote: 100 μL of purified recombinant protein (at approximately 2 mg/mL) were loaded onto a sizeexclusion chromatography column (PL1580-3301, Agilent) in the phosphate buffer (20 mM Tris ...
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bioRxiv - Genomics 2021Quote: ... Final quality control of the purified RNAs was performed using the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip Kit (Agilent) and the Qubit Fluorometer with the Qubit RNA BR (Broad-Range ...
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bioRxiv - Genetics 2020Quote: ... The final purified product was then quantified and qualified using the TapeStation DNA screen tape (Agilent, Santa Clara, CA, USA). For exome capture ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The UV-visible absorption spectra of wild and mutant purified visual pigments were recorded using a Cary 4000 double beam spectrophotometer (Agilent). The photopigments were photo-excited with light from a fiber optic lamp (Dolan-Jenner ...
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bioRxiv - Cell Biology 2020Quote: ... including glutathione S-transferase (GST)-NEPH1 cytoplasmic domain (CD) and GST-NEPHRIN CD were expressed and purified from Escherichia coli BL21 cells (Stratagene). Purified phosphorylated GST-NEPH1 was expressed and purified from TKB1 cells (Stratagene) ...
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bioRxiv - Physiology 2021Quote: ... pH (using the indicator dye purified m-cresol purple [34] in an Agilent 8453 spectrophotometer (Agilent, Santa Clara, CA, USA)) ...
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bioRxiv - Microbiology 2020Quote: ... We produced the purified 16S V4 rDNA by amplification of the V4 region of Escherichia coli XL 10-gold (Agilent) using TaKaRa Taq R001 AM kit (Clonetech ...
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bioRxiv - Immunology 2020Quote: ... Amplified products were solid phase reversible immobilization (SPRI) bead-purified and evaluated by Fragment Analyzer (Agilent, Santa Clara, CA, USA). Twenty-five percent of the cDNA volume was subjected to fragmentation and double-sided SPRIselect (Beckman Coulter ...
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bioRxiv - Genomics 2021Quote: ... 1ul of the purified amplicons were run on DNA1000 Agilent bioanalyzer (Cat. No. 5067-1504, Agilent, Santa Clara, California, USA). Further ...
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bioRxiv - Biochemistry 2022Quote: ... to remove their N-terminal 6×His-tag according to our previous procedure [3,18] and purified by HPLC using an analytical C18 reverse-phase column (Zorbax 300SB-C18, 4.6 × 250 mm; Agilent Technologies). For those C4ORF48-fused LEAP2 precursors ...
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bioRxiv - Biochemistry 2022Quote: ... The refolded mature LEAP2s were then purified by HPLC using an analytical C18 reverse-phase column (Zorbax 300SB-C18, 4.6 × 250 mm; Agilent Technologies). Purity of the mature LEAP2s was analyzed by HPLC and their identity was confirmed by electrospray mass spectrometry.
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bioRxiv - Molecular Biology 2022Quote: ... 2 μl of each purified final library was run on an Agilent TapeStation HS D1000 ScreenTape (Agilent Technologies, 5067-5584). The libraries were quantified using the Quant-iT 1X dsDNA HS kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The peptides were then purified and concentrated using OMIX Tips C18 reversed-phase resin (Agilent Technologies, Santa Clara, CA, USA). The peptides were dehydrated in a vacuum centrifuge and analysed by NanoLC-MS/MS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Amplified and purified sequencing libraries were analyzed on an Agilent 2100 Bioanalyzer (High sensitivity DNA analysis kit, Agilent, 5067-4626), pooled in equimolar amounts (2.47 ng/μL) ...
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bioRxiv - Immunology 2023Quote: Fluorophore-conjugated (or biotinylated) peptides were purified via reversed phase chromatography on a C18 column (Pursuit XRs C18 column, Agilent) to separate peptide-dye conjugates from unconjugated peptides or dyes ...
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bioRxiv - Cancer Biology 2023Quote: ... The purified capture products was then amplified using the SureSelect Post-Capture Indexing forward and Index PCR reverse primers (Agilent) for 12 cycles ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Captured samples for shotgun sequencing were indexed using universal iP5 and unique iP7 indices (Meyer & Kircher, 2010) and purified with AMPure XP beads (Agilent). The libraries were then pooled in equimolar ratios ...
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bioRxiv - Microbiology 2023Quote: ... PCR products are then purified and checked for fragment size distribution on Fragment Analyzer using HS NGS Fragment Kit (1-6000bp) (Agilent).
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bioRxiv - Biochemistry 2023Quote: ... 10μL of purified protein was injected to a C-18 column (Agilent Zorbax 300SB-C18, 5 μM, 2.1 x 150mm) without dilution or buffer exchange ...
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bioRxiv - Genomics 2023Quote: ... Purified RNAs were quantified by Qubit and quality was assessed using RNA Pico Assay kit with Bioanalyzer (Agilent Technologies, USA). chrRNA were then subjected to library preparation using True-seq stranded total RNA library prep gold kit (Ref#220599 ...
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bioRxiv - Biophysics 2023Quote: ... reduced with DTT and purified by reversed-phase high-performance liquid chromatography (RP-HPLC) using a ZORBAX 300SB-C3 column (Agilent) with flow rate of 2.5 ml/min starting at 95% RP-HPLC solvent A (99.9% H20 ...
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bioRxiv - Biochemistry 2023Quote: Each mitochondrial rRNA was purified by using reversed-phase LC through a PLRP-S 4000Å column (4.6 × 150 mm, 10 μm, Agilent Technologies). After applying the mixture of rRNAs from the purified ribosome or total mitochondrial RNA to the column ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR-amplified library was purified using Ampure XP beads and its quality was assessed on a Bioanalyzer 2100 system (Agilent). Diluted libraries were clustered on a pair-read flowcell and sequenced using a NovaSeq 6000 system (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... and the quality of the purified RNA was assessed with an RNA 6000 Nano Chip on a Bioanalyzer 2100 (Agilent) and by 1.2% agarose gel electrophoresis ...
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bioRxiv - Bioengineering 2024Quote: ... The size of purified DNA was profiled with the genomic DNA ScreenTape assay (range: 200∼60000 bp) via the 4200 TapeStation system (Agilent).
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bioRxiv - Cell Biology 2024Quote: ... of WRN was amplified by PCR from the pCMV-FlagWRN plasmid or from the pCMV-FlagWRNS1133D mutant The PCR product were subsequently purified and sub-cloned into pGEX4T-1 vector (Stratagene) for subsequent expression in bacteria as GST-fusion proteins ...