Labshake search
Citations for Agilent :
2151 - 2200 of 2474 citations for Ciprofloxacin Hcl 100 Ug Ml In Methanol 2 3 Carboxyl 13C3 99%; Quinoline 15N 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2021Quote: ... The cells were washed twice with 0.5 ml Seahorse XF DMEM medium pH 7.4 (Agilent) and then incubated in a CO2-free incubator at 37 °C (or 24°C for S2 cells ...
-
bioRxiv - Immunology 2021Quote: ... beads were incubated with 5 μg/ml FITC-conjugated rabbit anti-human C1q (Dako, F0254).
-
bioRxiv - Molecular Biology 2023Quote: ... Mouse and/or rabbit IgG (1:1000, 1μg/mL, diluted in antibody diluent, Agilent Technologies) served as negative control ...
-
bioRxiv - Pathology 2023Quote: ... Antigen retrieval was carried out using Proteinase K (5 μg/ml, 7 mins, Dako, UK). Sections were rinsed in running tap water for 5 min ...
-
bioRxiv - Plant Biology 2020Quote: ... RNA was quantified and evaluated for purity using a Nanodrop Spectrophotometer ND-100 (NanoDrop Technologies, Willington, DE) and Bioanalyzer 2100 (Agilent, Santa Clara, CA). For each sample ...
-
bioRxiv - Developmental Biology 2020Quote: ... sections were permeabilized with 0.25% TritonX-100 in PBS and autoclaved (121°C, 10 min) with Target Retrieval Solution (Agilent Technologies, Santa Clara, California). Sections were incubated with Blocking One Histo (Nacalai Tesque Inc. ...
-
bioRxiv - Bioengineering 2019Quote: ... The following primary antibodies were used for immunocytochemistry experiments: rabbit anti-glial fibrillary acidic protein (GFAP) (DAKO, 1:100, Z-0334, Lot #2002332), mouse anti-vascular endothelial (VE)-cadherin (Abcam ...
-
bioRxiv - Genomics 2021Quote: ... 100 nucleotides) was verified by fluorescent capillary electrophoresis using an Agilent 2100 Bioanalyzer (Agilent RNA 6000 pico kit, Agilent, Santa Clara, CA, USA).
-
bioRxiv - Plant Biology 2020Quote: ... Samples were injected on an Infinity Lab Poroshell 120 Z-HILIC column (2.7 μm, 100 x 2.1 mm; Agilent technologies, Santa Clara, CA, USA). All LC-MS/MS analyses were performed using a Shimadzu (UFLCXR ...
-
bioRxiv - Plant Biology 2020Quote: ... A 5 μl sample was injected on the Infinity Lab poroshell 120 Z-HILIC column (2.7 μm, 100 x 2.1 mm; Agilent technologies, Santa Clara, CA, USA) and the metabolites were eluted with an increasing gradient of acetonitrile ...
-
bioRxiv - Plant Biology 2022Quote: ... Metabolites were then re-suspended in 100 μL distilled water and soluble sugars and sugar alcohols (fructose, glucose, sucrose, mannitol, sorbitol) were quantified by HPLC (Agilent 1260 Infinity II) using an 8 μm Hi-Plex Ca column (7.7 mm x 300 mm ...
-
bioRxiv - Biochemistry 2021Quote: ... Analyses were performed on an Agilent Infinity 1260 HPLC system equipped with a 300 × 7.5 mm PLgel SE-HPLC column (particle size 5 μm, pore size 100 Å) (Agilent Technologies, Waldbronn, Germany). Dichloromethane was used as eluent at a flow rate of 1.00 ml/min ...
-
bioRxiv - Cell Biology 2022Quote: ... Medium was changed 1-hour before the start of readout into 180 μL serum-free assay medium (Agilent Seahorse XF DMEM; 103575-100) supplemented with 4mM L-glutamine (Corning ...
-
bioRxiv - Biochemistry 2022Quote: ... HILIC analysis was predominantly performed on the 6520 LC-MS using a Poroshell 120 HILIC-Z column (Agilent, 2.7 μm, 2.1 × 100 mm) with water and 9:1 ACN:water ...
-
bioRxiv - Pathology 2021Quote: The primary antibodies were incubated at the following concentrations in antibody diluent (PBS + 1% BSA (Fisher BioReagents, BP1605-100) with 0.1% Tween™ 20 (Fisher BioReagents, BP337-500): PanCK (Dako, Z0622, 1:200), Cleaved Caspase 3 (Cell Signaling ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in 100 µl of 0.5% BSA in PBS and incubated with mouse anti-human CD31 (Agilent Dako, M0823, clone JC70A) antibody-coated magnetic beads (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... Cells cultured on 100 mm plates were washed once with PBS and cell monolayers were irradiated with 100 mJ in a UV-Stratalinker 2400 (Stratagene, San Diego, CA) with the plate lid off or on to generate the mock control ...
-
bioRxiv - Cell Biology 2022Quote: ... 120 μL of supernatant was removed from each tube and filtered using 0.2 μm polyvinylidene fluoride filter (Agilent Technologies P/N: 203980-100) and collected via 6,000 rcf centrifuge for 4 minutes ...
-
bioRxiv - Pathology 2022Quote: ... and a monoclonal antibody directed against the alpha isoform of smooth muscle actin at a working dilution of 1/100 (a-SMA, clone 1A4, n M0851; Dako, Denmark A/S). Alligator skeletal muscle was used as positive control for anti-desmin antibody (Supplemental figure 3A) ...
-
bioRxiv - Plant Biology 2022Quote: ... Chlorophyll content was then determined using 100% ethanol extraction (Ritchie, 2006) and analyzed with spectrophotometer (BioTek PowerWave Microplate Reader, Agilent, Santa Clara, CA). For other experiments ...
-
bioRxiv - Plant Biology 2022Quote: ... Ten microliters of the reconstituted samples were loaded onto a 3.0 × 100 mm 1.8 μm ZORBAX Eclipse XDB-C18 column (Agilent Technologies, Santa Clara, CA, USA) and the phytohormones were eluted within 22.0 minutes ...
-
bioRxiv - Immunology 2023Quote: ... Conjugation efficacy and conjugated IgG concentration was measured by absorbance spectroscopy using Agilent Cary 100 UV-vis spectrophotometer (Agilent Technologies, Santa Clara, CA).
-
bioRxiv - Plant Biology 2023Quote: ... Amino acids were separated on an Infinity Lab Poroshell 120 Z-HILIC column (2.7 µm, 100 x 2.1 mm; Agilent Technologies, Santa Clara, CA, USA) and detected in the positive ion mode of MS (Czajka et al. ...
-
bioRxiv - Evolutionary Biology 2023Quote: Enzymes were assayed at 25 °C by recording the change in absorbance at 340 nm for 300 seconds on a Cary 100 Bio (Agilent, Santa Clara, CA) in 50 mM Tris ...
-
bioRxiv - Cancer Biology 2023Quote: ... The oxygen consumption rate (OCR) assay was performed using the XF long-chain fatty acid oxidation (LCFA) stress test kit (Agilent, cat# 103672-100) and Seahorse XFe24 Analyzer was used to investigate the long-chain fatty acid OCR according to the manufacturer’s protocol.
-
bioRxiv - Cancer Biology 2023Quote: ... A multichannel pipette was used to transfer 50 µL of the cell suspension (1.5×105 cells) evenly to each well of the PDL plates (Seahorse Bioscience, cat: #103798-100). The cells were centrifuged at 200 rcf (zero braking ...
-
bioRxiv - Cell Biology 2023Quote: ... before permeabilization with 0.5% Triton X-100 in PBS and stained with primary antibodies: anti-Ki67 (M7240, Agilent Dako, Santa Clara, CA, USA) diluted at 1/50 and anti-Poly-ubiquitin purchased from ENZO (ENZ-ABS840 ...
-
bioRxiv - Microbiology 2024Quote: ... of 0.1 and incubated for 100 h at 30 °C with linear shaking at 567 cycles per minute using an EPOCH microplate reader (Agilent, Santa Clara, CA). The OD600 was measured continuously.
-
Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... Acute 10 μM glutamate injection was applied to four replicate wells/donor/experiment during the mitochondrial stress test (Agilent Technologies, Cat#: 103015-100). The mitochondrial stress test was performed according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2021Quote: ... 7.8 mm ID x 300 mm column equilibrated in GF150 buffer (25 mM HEPES-KOH, 150 mM KCl, 2 mM MgCl2) plumbed into an HPLC (Agilent 1100). Molecular masses were analyzed by an inline SEC-MALs system (Wyatt Technology ...
-
bioRxiv - Microbiology 2020Quote: ... The SARS-CoV-2 RBD constructs carrying point mutation were generated by following the standard protocol from QuikChange® II XL Kit (Agilent). The cloned genes were sequenced to confirm that no errors had accumulated during the PCR process ...
-
bioRxiv - Cell Biology 2020Quote: ... The cell viability was assessed by measuring the absorbance at 570 nm wavelength using Epoch 2 microplate spectrophotometer (Agilent technologies. USA).
-
bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies against proliferating cell nuclear antigen (PCNA-1, 1/200, sc-7907, Santa Cruz; PCNA-2, 1/200, M087901, Dako) were applied overnight at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... TUJ1 (Covance) and CMTM5 (custom made by Pineda, Berlin, Germany Table 2) antibodies were diluted in antibody diluent (DAKO, Hamburg, Germany) and incubated for 48 hours at 4°C in a dark ...
-
bioRxiv - Neuroscience 2021Quote: Purpose built rodent specific coils (circular coil 8mm in diameter and height-see [2]) controlled by an arbitrary waveform generator (Agilent 335141B) connected to a bipolar power supply (Kepco BOP 100-4M ...
-
bioRxiv - Genomics 2019Quote: ... fragments with UMIs were generated using 200 ng starting material (purified by ethanol precipitation) in 2 cycles of PCR with Herculase II Fusion DNA Polymerase (Agilent Technologies) using an equimolar mixture of P023poolseqNN-primers (1 mM final concentration ...
-
bioRxiv - Developmental Biology 2019Quote: ... truncated and deletion versions of RAB13 3’UTR were generated by PCR using 0.3 μM sequence-specific primers (Extended Data Table 2) and Platinum Pfx DNA polymerase or using QuikChange II Site-Directed Mutagenesis kit (Agilent Technologies) following the manufacturer’s instructions and introduced into the pcDNA3-HBB-24XMS2SL-MCS using the NheI and XhoI sites.
-
bioRxiv - Genetics 2021Quote: ... Samples with a 260/280 nm absorbance ratio > 1.8 and a 260/230 nm absorbance ratio > 2 were labeled and hybridized to the Tomato Gene Expression Microarray 4 × 44K (Agilent Technologies) as previously described (Coppola et al. ...
-
bioRxiv - Microbiology 2021Quote: ... The RNA was then cross-linked to positively charged membranes using a UV-crosslinker (Stratagene UV Stratalinker 2400, 2×240 mJoules) and stained with methylene blue (SERVA ...
-
bioRxiv - Systems Biology 2021Quote: ... The staining was performed according to the manufacturer’s procedure with EnVision G|2 Doublestain System Rabbit/Mouse (DAB+/Permanent Red) kit (Dako/Agilent K5361) on the Dako Autostainer ...
-
bioRxiv - Biochemistry 2020Quote: ... Radioactivity was detected using an in-line Lablogic Radiodetector and the analysis was calibrated using a co-injected 2-aminobenzamide-labeled dextran ladder (ProZyme/Agilent) detected on a Dionex fluorescence detector.
-
bioRxiv - Biochemistry 2021Quote: Plasmids encoding AlgE7 variants (Table 2) were constructed based on wild type AlgE7 (plasmid pBG27) (12) using the QuikChange™ Site-directed Mutagenesis Kit (Stratagene/Agilent) or Q5 site directed mutagenesis (New England Biolabs) ...
-
bioRxiv - Microbiology 2020Quote: ... for 2hrs at 40°C and then incubated over-night at 4°C on a rotating wheel with 2 μg of anti HBc antibody (Dako B0586). Immune-complexes were captured with protein A/G magnetic beads ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were further diluted with ultrapure water to a final concentration of 2% HNO3 and subjected to ICP-MS on Agilent 7700 ICP-MS (Agilent Technologies). ICP-MS runs were calibrated with high-purity iron standard solution (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2020Quote: ... slides with PCa tissue samples (n=144) were incubated at 60°C for 2 hours followed by target retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
-
bioRxiv - Cancer Biology 2021Quote: ... W503F (PBD, polo-box domain 1) and H629A, K631M (PBD, polo-box domain 2) were generated by site-directed mutagenesis (Agilent Technologies) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... embedded in paraffin, and automatically stained for SARS-CoV-2 (2019-nCoV) Nucleocapsid (SINO BIO, #40143-R019) or for fibrin (DAKO, #A0080) through LEICA BOND RX 1h room- temperature (RT ...
-
bioRxiv - Cancer Biology 2020Quote: ... at 4°C overnight followed by incubation with Dako EnVision+System-HRP Labelled Polymer Anti-Mouse (Aglient Dako, Cat # K400111-2) for 60 min at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... by placing the virus for 2 minutes in a UV Stratalinker 2400 equipped with 365 nm long-wave UV bulbs (Stratagene, USA). UV-inactivation was confirmed by lack of cytopathic effect on BSC-1 cells infected with i-VACV for up to 3 days (data not shown).
-
bioRxiv - Biochemistry 2021Quote: ... was added to a final concentration of 0.5% along with 2 µL of PNGase F Ultra (Agilent Technologies, Santa Clara, CA) and incubated for 1 hour at 37 °C before subsequent 2-AB/2-AA labelling ...