Labshake search
Citations for Agilent :
2051 - 2100 of 2500 citations for Tubulin inhibitor 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... for 1 h at RT and incubated overnight at 4°C with 1:100 dilution of mouse anti-Ki67 antibody (Novocastra, Concord, ON, Canada) in antibody diluent (DAKO). Next ...
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Engineering of membrane complex sphingolipids improves osmotic tolerance of Saccharomyces cerevisiaebioRxiv - Bioengineering 2019Quote: ... The dried samples were sent to the Profleader Institute for complex sphingolipids analysis and solubilized in dichloromethane-methanol (2:1,vol/vol) before analysis by UHPLC-QTOF-MS (Agilent) analysis (Fig ...
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bioRxiv - Biochemistry 2019Quote: ... the relevant tetrapeptide motifs were inserted into iBox-PAK4cat within the pXJ40 vector [1] using the Quikchange II Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... 1 µL was injected in splitless mode through a GC inlet liner (ultra inert, splitless, single taper, glass wool, Agilent) onto a DB-5MS column (30 m × 0.25 mm ...
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bioRxiv - Cancer Biology 2019Quote: ... 3-4 μm slides were cut and stained for MLH1 (Monoclonal Mouse Anti-Human; Clone ES05, dilution 1 : 10,Dako), MSH2 (Monoclonal Mouse Anti-Human ...
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bioRxiv - Biochemistry 2020Quote: UV-visible spectra were recorded in 1-cm optic path quartz cuvettes under aerobic conditions on a Cary 100 Uv-vis spectrophotometer (Agilent) and under anaerobic conditions in a glove box on a XL-100 Uvikon spectrophotometer equipped with optical fibers.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were incubated with 1:200 (v/v) mouse monoclonal anti-AX AO3.2 [16] and 1:2000 (v/v) anti-mouse IgG-HRP (Dako Cytomation) diluted in 1%(w/v ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 μL of this mixture was injected into an Agilent 7890A Gas Chromatograph with 5977A MSD (Agilent, Santa Clara, CA) using an Agilent HP-5MS (30 m length x 0.25 mm i.d ...
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bioRxiv - Cancer Biology 2019Quote: ... Devices were then washed with 1× PBS solution for 3 times and mounted on glass slides using fluorescent mounting medium (Dako) and the coverslips were sealed using nail polish ...
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bioRxiv - Immunology 2020Quote: ... and bound mAbs were detected with rabbit anti-human IgG gamma chain-specific/HRP (Agilent: cat. P0214, 1:15,000 dilution).
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bioRxiv - Plant Biology 2020Quote: ... The dried peptides were dissolved in 1% TFA solution to perform zip-tip cleanup using C-18 Omix tips (Agilent). Digests were dried in a speedvac as elution-2 (E2 ...
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bioRxiv - Genomics 2021Quote: ... Between 1 and 5 ng of the purified digested DNA was loaded on a High Sensitivity DNA chip (Agilent Technologies) and run on an Agilent 2100 Bioanalyzer System ...
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bioRxiv - Neuroscience 2020Quote: ... we used horseradish peroxidase (HRP)-conjugated goat anti-rabbit and goat anti-mouse secondary antibodies (diluted 1:200 in PBS; DAKO) to bind the primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Stained cells suspensions were counted in 20 μl sample volume at medium flow rate (60 μl min−1) using an ACEA NovoCyte Green flow cytometer (OMNI Life Science Agilent). The SYBR Green I signal was measured in the FITC-channels of the instrument ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were heated from 25 °C to 96 °C at 1 °C/min using an Mx3005P qPCR machine (Agilent Technologies). The dye was excited at 492 nm ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sections were thoroughly washed with 0.3% Triton-TBS followed by antigen retrieval with target retrieval solution citrate pH6 (1:10, Dako S2031) for 20 minutes at 80°C ...
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bioRxiv - Physiology 2019Quote: The oxygen consumption rate (OCR) of HL-1 cardiomyocytes was measured using the XFp Cell Mito Stress Test by Agilent Seahorse24 ...
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bioRxiv - Systems Biology 2020Quote: ... lymphatic tissue was cut into 1-mm pieces with a sterile scalpel and disaggregated mechanically into a single-cell suspension using the Medimachine (DAKO) equipped with sterile cartridges holding rotating knives (Medicons ...
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bioRxiv - Developmental Biology 2019Quote: ... for one hour at room temperature and incubated overnight at 4°C with 1:200 dilution of mouse anti-Ki67 antibody (Novocastra, Concord, ON, Canada) in antibody diluent (DAKO). Next ...
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bioRxiv - Zoology 2020Quote: ... Slides were rinsed with PBS (pH = 7.4) and then subjected to antigen retrieval procedure using proteinase K 1:30 (Dako, Denmark). Nonspecific binding sites were blocked with 2% BSA for 30 min in a humid chamber at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies used for immunoblotting were horseradish peroxidase-coupled goat anti-mouse and goat anti-mouse IgG (Dako, 1:5000). Secondary antibodies used for immunofluorescence were Alexa fluorophore (488 ...
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bioRxiv - Molecular Biology 2019Quote: ... High mass accuracy was achieved by infusion of 1:4 diluted ESI low concentration tune mix (Agilent Technologies, Waldbronn, Germany) at the start of each chromatographic run ...
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bioRxiv - Plant Biology 2019Quote: ... The digest was acidified by adding formic acid to a final concentration of ~ 1 % and desalted using OMIX C18 pipette tips (10 – 100 μL, Agilent). C18 desalting tips were first activated by twice aspiring and discarding 200 μl buffer B2 (0.1 % formic acid ...
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bioRxiv - Genomics 2019Quote: ... The prepared library was quantified using Qubit and validated for quality by running an aliquot (1 μl) on High Sensitivity DNA Kit (Agilent) which showed expected fragment distribution in the range of ~250–500 bp ...
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bioRxiv - Bioengineering 2019Quote: ... The primary antibodies sources and dilutions were as follows: rabbit poly-clonal anti-glia fibrillary acidic protein (GFAP; 1:500; DAKO), mouse monoclonal anti-Neuronal Class III B-Tubulin (TuJ1 ...
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bioRxiv - Biochemistry 2021Quote: ... and transfected with a DNA mixture containing either the 4xNFAT or the 7xAP-1 promoter-Firefly luciferase reporter (100 ng; Stratagene), along with Renilla luciferase (50 ng of pGL4.70[hRluc] or 1 ng of pRL-null ...
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bioRxiv - Neuroscience 2021Quote: ... free floating sections were blocked with 3% donkey serum and incubated overnight with either rabbit anti-GFAP (1:10000, Dako), rat anti-CD68 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and incubated with biotinylated rabbit anti-rat IgG for 1h (1:200, DAKO Cytomation A/S, Denmark). Following avidin-biotin-peroxidase complex (Vector laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... 25 and 50 ppb with Sc-45 (ICP-MS internal standard mix 1 ug/mL in 2% HNO3, Agilent Technologies) as the internal standard for Cu-63.
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bioRxiv - Developmental Biology 2021Quote: ... Half of the cDNA was amplified for 17 PCR cycles and a 1:4 dilution of the resulting library was assessed by Agilent Bioanalyzer High Sensitivity DNA kit ...
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bioRxiv - Plant Biology 2021Quote: ... The filtrate was passed through a 1 g phase C18 silica-bonded non-polar column (HF Mega Bond Bond Elut C18, Agilent, 5301 Stevens Creek Blvd ...
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bioRxiv - Developmental Biology 2021Quote: ... and the sections were incubated for 1 h at room temperature with biotinylated IgG anti-mouse secondary antibody which is produced in rabbit (E 0354 -Dako Cyt ...
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bioRxiv - Genomics 2021Quote: ... and a second 1 μl aliquot was used to determine molecule length with an Agilent Genomic Tape (Agilent, Cheadle, UK) on an Agilent TapeStation (Agilent).
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation for 45 minutes at room temperature in 1:100 dilution of rabbit anti-goat immunoglobulins/HRP (Dako, P0160 or ready-to-use goat anti-rabbit HRP labelled polymer (Dako ...
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bioRxiv - Cancer Biology 2021Quote: ... MCF10A shCtrl and shEIF3E cell lines were transfected in 100 mm tissue culture plates with 5 μg of DNA at a ratio of 1:50 of a T7-tagged PRP4K expression vector (16) or pBluescript SK-(Stratagene/Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... and RNA quality and integrity was assessed though gel electrophoresis (1% agarose gel) and the Agilent Bioanalyzer 2100 (Agilent Technologies). Labelling and hybridization were performed ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were passed through an immobilized pepsin column (2mm 2cm) at 200 μl min−1 and the digested peptides were captured on a 2mm 1cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Microbiology 2021Quote: Immunofluorescent (IF) staining was performed using the following primary antibodies: polyclonal rabbit anti-HSV-1 (cross-reactive with HSV-2; Agilent), anti-Iba1 (Wako ...
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bioRxiv - Microbiology 2021Quote: Mutations were introduced into the HA plasmid of A/Hong Kong/1/1968 using a site-directed mutagenesis kit (QuikChange; Stratagene). 2:6 recombinant IAVs containing wt and modified HA of A/Hong Kong/1/1968 ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed in 4% PFA and sequentially stained with mAbs to NPM (anti-human/mouse nucleophosmin (NPM) (clone 376, dilution 1:50, Dako) and anti-mouse MPO (Millipore) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cy3-labelled probes were prepared from RNA using Low Input Quick Amp Labeling Kit 1-color (Agilent Technologies, #5190-2305) and hybridized with the custom microarrays (ID:085740 ...
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bioRxiv - Genomics 2021Quote: ... The shape and the mean fragment size of the libraries were determined on a 5200 Fragment Analyzer using the HS NGS Fragment Kit 1-6000 bp (both Agilent). Enriched libraries were loaded with a final concentration of 10 pM on a MiSeq Flow Cell using v3 reagent chemistry for 2×150 cycles.
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bioRxiv - Biochemistry 2021Quote: ... Cys to Ala mutants of Flag-tagged DJ-1 were using QuikChange Ⅱ Site-Directed Mutagenesis kit (Agilent Technologies, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was carried out with the LSAB2 System-HRP (anti-rabbit/mouse LSAB2 Kit Dako Cat#K0679, dilution 1:100) or the VECTASTAIN Elite ABC HRP Kit (Vector Labs ...
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bioRxiv - Neuroscience 2020Quote: Total lysate and synaptoneurosomes isolated from cortex tissue of 1-month-old C57Bl/6 mice were UV crosslinked (100 mJ/cm2 for 2 cycles) using UV Stratalinker 2400 (Stratagene) and stored at −80°C until use ...
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bioRxiv - Genomics 2022Quote: ... and 1 μl was removed to assess tagmentation fragment size and yield by D5000 ScreenTape (Catalog No. 5067-5588, Agilent) on an Agilent Tapestation.
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bioRxiv - Cell Biology 2022Quote: ... Cells were cultured for 2-days in complete RPMI to allow for attachment to the culture dish and then for 1-hour in XF RPMI medium (Agilent) with 2.8 mM glucose ...
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bioRxiv - Biochemistry 2022Quote: ... Astrocytes were pre-incubated with 10 μM CGP-37157 or DMSO for 1 h and then plates were inserted in a XFe24 Seahorse Analyzer apparatus (#102238-100, Agilent). Cells were stimulated with 100 μM ATP ...
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bioRxiv - Biophysics 2022Quote: TTP was run on a tandem size exclusion chromatography - multi-angle light scattering detector by injecting 100 μL sample at a concentration of 1 mg/mL using a 1260 Infinity HPLC (Agilent). The column was pre-equilibrated in 0.1 μm filtered buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Bioengineering 2022Quote: ... and incubated overnight at 4 °C with primary antibodies for the glial fibrillary acidic protein (GFAP; 1:1000, Z0334, Dako), ionised calcium-binding adapter molecule 1 (IBA1 ...