Labshake search
Citations for Agilent :
2001 - 2050 of 3815 citations for 7 Propyl 3 trifluoromethyl 1 2 benzisoxazol 6 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... then followed by immunoblotting for ≤ 1 h with secondary antibodies: 1:5,000 anti-mouse linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Microbiology 2022Quote: ... then followed by immunoblotting for ≤ 1 h with secondary antibodies: 1:5,000 anti-mouse linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Immunology 2020Quote: ... followed by the incubation with horseradish peroxidase-labeled secondary antibody (1:10,000) for 1 h at room temperature (DAKO EnVision™+ System ...
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bioRxiv - Neuroscience 2021Quote: ... WAKO) antibodies were diluted at 1:250 and 1:1000 respectively with EnVision FLEX Antibody Diluent (K800621, Dako-Agilent) and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... WAKO) antibodies were diluted at 1:250 and 1:1000 respectively with EnVision FLEX Antibody Diluent (K800621, Dako-Agilent) and incubated overnight at 4°C ...
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bioRxiv - Genetics 2019Quote: ... the following primary antibodies were used at a 1:100 dilution: 1) anti-GH rabbit polyclonal antibody (Dako, A0570); 2 ...
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bioRxiv - Pathology 2020Quote: ... It was then incubated with horseradish peroxidase-labelled secondary antibodies (cat. no. sc-2020; 1:5,000; Santa Cruz Biotechnology, Inc.; and cat. no. K4003; 1:5,000; Dako; Agilent Technologies ...
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bioRxiv - Pathology 2020Quote: ... It was then incubated with horseradish peroxidase-labelled secondary antibodies (cat. no. sc-2020; 1:5,000; Santa Cruz Biotechnology, Inc.; and cat. no. K4003; 1:5,000; Dako; Agilent Technologies ...
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bioRxiv - Genetics 2020Quote: ... We determined efficiency of each reaction using the equation Efficiency = −1+10(−1/slope of standard curve) (Agilent Genomics). We additionally calculated ratios of transcript abundance to determine expression levels of one gene relative to another gene and to account for any differences in underlying mRNA levels among individuals ...
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bioRxiv - Microbiology 2020Quote: ... The MUF production (fmol MUF cell-1 h-1) was measured with a Cary Eclipse spectrofluorometer (FL0902M009, Agilent Technologies) at 359 nm (excitation ...
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bioRxiv - Neuroscience 2022Quote: ... The following day slides were rinsed and incubated with horseradish peroxidase labelled secondary antibodies for 1 hour and developed using 3,3′-diaminobenzidine (DAB, 1:50, DAKO) for 5 minutes ...
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bioRxiv - Genetics 2022Quote: The wild-type and mutant BRD-1-BRC-1 chimeras were expressed in BL21-CodonPlus (DE3)-RIPL cells (Agilent). The cells were grown at 37°C until OD600 0.6 and were induced by 0.2mM isopropyl-β-D-thiogalactoside in the presence of 100μM ZnCl2 at 37°C for 6 hrs ...
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bioRxiv - Cancer Biology 2022Quote: ... was followed by incubation with streptavidin conjugated to horseradish peroxidase (1:200; 1.25% NGS/1% BSA in PBS; 30 min) (#P0397, DakoCytomation). For visualization DAB (#D5905 ...
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bioRxiv - Neuroscience 2023Quote: ... prior to incubation in primary antibodies (anti-YKL-40, Abcam ab77528, 1:1,000; anti-C3d, Agilent A0063, 1:1,000). Sections were incubated in primary antibodies for one hour at room temperature prior to washing and probing with the universal probe and polymer-HRP components of the Mach 4 Polymer kits (CellPath ...
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bioRxiv - Neuroscience 2023Quote: ... followed by washing in TBST (0.1%) and a 1 h incubation in secondary antibody (goat a-mouse-HRP: 1:10000, P0447, Dako). After washing ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating brain slices were incubated in a solution of anti-Iba1 antibody (1:2000; Fuji film-Wako) or anti-GFAP antibody (1:10,000; Agilent-Dako) at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... anti-Gfap rabbit polyclonal antibody (1:2,000 [brain] or 1:4,000 [spinal cord]; Z0334; Dako/Agilent, Santa Clara, CA); and anti-Iba1 rabbit polyclonal antibody (1:2,000 [brain] or 1:4,000 [spinal cord] ...
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bioRxiv - Neuroscience 2024Quote: ... anti-Gfap rabbit polyclonal antibody (1:2,000 [brain] or 1:4,000 [spinal cord]; Z0334; Dako/Agilent, Santa Clara, CA); and anti-Iba1 rabbit polyclonal antibody (1:2,000 [brain] or 1:4,000 [spinal cord] ...
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bioRxiv - Cancer Biology 2021Quote: ... the membranes were washed 3X with TBST followed by incubation with HRP-conjugated secondary antibodies (Rabbit Cytiva/Amersham NA934; Mouse Agilent/Dako P026002-2) for one hour at room temperature ...
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bioRxiv - Genetics 2020Quote: ... and 0.5 mL was pipetted into individual 2 mL glass vials fitted with 9mm PTFE lined caps (Agilent Crosslab, Santa Clara, CA, USA). The hexane was evaporated under a nitrogen gas flow ...
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bioRxiv - Cell Biology 2020Quote: ... RNA quality of all samples (n=7 time points and n=3 experiments from independent passages) was evaluated by both Nanodrop for (A260/280 >2) and Bioanalyzer 2100 High Sensitivity RNA Analysis chips (Agilent, Cat. 5067-1513) which displayed intact RNA integrity (RIN >9) ...
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bioRxiv - Developmental Biology 2021Quote: Immunofluorescence microscopy was performed on de-paraffinized tissue sections that received heat-induced antigen retrieval using Target Retrieval Solution (Agilent Dako, S169984-2). After washing and permeabilization in TBS-T universal buffer (0.2% Triton X-100 in tris-buffered saline) ...
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bioRxiv - Genomics 2020Quote: ... a plasmid encoding the replicases of AAV serotype 2 and the capsid of AAV serotype 9) and pHelper (Agilent Technologies, lab reference pZMB0088) which provides AAV adenoviral helper functions ...
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bioRxiv - Biochemistry 2019Quote: ... in 200μl of dichloromethane containing 200 ng of 2-tetradecyl acetate (internal standard) in 2ml glass vials with polytetrafluoroethylene-coated caps (Agilent, Santa Clara, California). After one hour ...
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bioRxiv - Biochemistry 2019Quote: ... solvent A) and methanol:isopropanol (8:2 v/v, solvent B) was generated by an Infinity II 1290 UPLC (Agilent, Santa Clara, CA, USA) and with a constant flow rate of 600 μL min−1 (0 min ...
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bioRxiv - Genomics 2020Quote: ... Chromium Genome Reagents Kit Version 2 User Guide) and size and concentration determined using an Agilent 2100 Bioanalyzer DNA 1000 chip (Agilent Technologies, CA, USA). Libraries were then sequenced on an Illumina NovaSeq 6000 System following the manufacturer’s protocols (Illumina ...
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bioRxiv - Bioengineering 2021Quote: ... samples were subjected to a treatment with 0.3% Sudan black solution for 10 min prior to blocking for 2 h (Dako Blocking Solution X0909; Agilent Technologies, Santa Clara, CA). Immunofluorescence staining was then performed using antibodies to detect CD31 (dilution 1:250 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Amino acid substitutions or deletions were introduced into the pSARS-CoV-2-Strunc expression vector by site-directed mutagenesis (Stratagene, La Jolla, CA) using mutagenic oligonucleotides as follow:
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bioRxiv - Cell Biology 2021Quote: Both PCRs were performed in 100 µl with the 2 µl of the Herculase II Fusion DNA Polymerase from Agilent (ref. no. 600675) according the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... 1 and 3.5 μm diameter fibers were manufactured from 2 and 5 wt% of high molecular weight polystyrene (MW: 15,000,000 g/mol, Agilent Technologies, Santa Clara, CA, USA) and equally spaced at approximately 60 μm ...
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bioRxiv - Genetics 2022Quote: ... and 0.5 mL was pipetted into individual 2 mL glass vials fitted with 9mm PTFE lined caps (Agilent Crosslab, Santa Clara, CA, USA). The hexane was evaporated under a nitrogen gas flow ...
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bioRxiv - Neuroscience 2020Quote: ... and heat-induced antigen retrieval was performed using 10mM sodium citrate solution (pH 6.0, pSer129) or citrate Target Retrieval Solution (Dako #S169984-2, pH 6.1) with 0.05% Tween-20 (conformation-specific ...
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bioRxiv - Physiology 2022Quote: ... All assays were run in duplicate under control conditions or in the presence of 5 mM DTT in a 96-well plate and changes in absorbance were tracked using a microplate reader (Synergy 2, BioTek/Agilent, Santa Clara, CA). This concentration was selected based on similar experiments in a previous study [15] ...
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bioRxiv - Immunology 2022Quote: FFPE tissue sections were deparaffinized and antigen retrieval was undertaken in 0.1 M citric acid buffer solution (Dako, target retrieval solution, S169984-2) using an electric pressure cooker (Russell Hobbs RHNHP401) ...
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bioRxiv - Biochemistry 2021Quote: ... and 12 were used as primary detection against T22 capture antibody) was an anti-tau polyclonal rabbit antibody (A002401-2) purchased from Agilent (Santa Clara, CA). A goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen □ retrieval was performed by incubating the tissue for 2 h at 70 °C in Target Retrieval Solution (Dako, Santa Clara, CA, USA) using a 1:10 dilution in demineralized water ...
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bioRxiv - Plant Biology 2023Quote: ... Ten-μl B314-2 sample was injected into an Eclipse XDB-C18 column (250 mm x 4.6 mm, 5 μm, Agilent, Santa Clara, CA, USA) and eluted with the same gradient solvent system described above ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 µg of the plasmid carrying the relevant recombinant provirus was co-transfected with 2 µg of plasmid pMD2.G (a kind gift from D. Trono) and 2 µg of plasmid PVPackgagpol (#217566 from Stratagene, La Jolla, CA) or plasmid pCMV-1′R8.74 (a gift from D ...
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bioRxiv - Genetics 2023Quote: ... Double-stranded cDNA was synthesised from 2 μg of total RNA using AccuScript High Fidelity 1st Strand cDNA Synthesis Kit (Stratagene, La Jolla, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and Cal27 were seeded at a density of 2×104 cells per well in a Seahorse XFe24 Cell Culture Microplate (102340-100; Agilent, Santa Clara, CA). Cells were incubated overnight for adherence in a culture media composed of DMEM and 10% FBS at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... The antigen retrieval step was repeated followed by second round staining for pan-CK using anti-pan-cytokeratin antibody (AE1/AE3 mouse monoclonal, Dako-Cat# M351501-2) followed by HRP-conjugated anti-mouse secondary antibody polymer (EnVision+ ...
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bioRxiv - Neuroscience 2023Quote: The AAV plasmid of human synapsin I promoter (hSyn)-tetracycline-controlled transactivator 2 (tTA2) was constructed by subcloning the DNA fragments containing hSyn and tTA2 into pAAV-MCS (Agilent Technologies, CA, USA). The AAV vector was produced as described previously (Ebina et al. ...
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bioRxiv - Microbiology 2023Quote: ... Plate was shaken continuously at 30°C and OD600 was read every 20 minutes in a BioTek Epoch 2 Microplate Spectrophotometer (Agilent, Santa Clara, CA). MMC (0.5 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... The AAV plasmid of human synapsin I promoter (hSyn)-tetracycline-controlled transactivator 2 (tTA2) was constructed by subcloning the DNA fragments containing hSyn and tTA2 into pAAV-MCS (Agilent Technologies, CA, USA). The AAV vector was produced as described previously15,35,55 ...
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Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... Coverslips were then incubated with 1X DAPI for 5 min before mounting on slides using DAKO mounting media (Agilent Technologies, Cat #S302380-2). Images were captured at 60X or 100X using a Nikon C2+ confocal microscope coupled to NIS-Elements AR software (Nikon).
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bioRxiv - Microbiology 2023Quote: ... followed by sample measurements, all performed in duplicates using the OT-2 robot (Opentrons, Long Island City, NY) and BioTek Synergy (Agilent Technologies, Waldbronn, Germany). The acquired data were evaluated using the Shiny application based on the determined standard curves for accurate quantification [62].
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bioRxiv - Microbiology 2024Quote: ... The growth was monitored by measuring the optical density at 600 nm (OD600) in an Epoch 2 plate reader (Agilent, Santa Clara, CA). The growth rates were calculated using the R package growthcurver (32).
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bioRxiv - Cell Biology 2020Quote: ... The cells were incubated with anti-tubulin (Thermo PA1-41331; dilution 1:250) and K9JA (Dako A0024; dilution 1:500) antibody ...
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bioRxiv - Cell Biology 2020Quote: ... then probed for 1 hr at room temperature with HRP-conjugated goat anti-mouse IgG secondary antibodies 1:500 in PBS (Dako, Agilent Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated overnight at 4°C with primary antibodies (rabbit anti-TMEM98, Proteintech, 1:1000; rabbit anti-FLAG polyclonal, Sigma, 1:2000; mouse anti-FLAG monoclonal M2, Stratagene 1:2000 ...