Labshake search
Citations for Agilent :
2001 - 2050 of 4557 citations for 6 chloro 2 N 2 N diethyl 4 N propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... The coding sequence of FAM104A isoform 5 was mutated using the QuikChange XL II kit (Agilent) with primers FAM104A_NL-RR_fwd (5’-cct cta ctt cca cat ccg cca gac ccg cag gga ggc cca ctt cc) ...
-
bioRxiv - Microbiology 2023Quote: ... with GenePix Pro 6.1 (AutoPMT; pixel size of 5 µm) and analysed with GeneSpring 14.8 (Agilent).
-
bioRxiv - Zoology 2024Quote: ... We used an HP-5 column (30 x 0.25 mm ID X 0.25 μm, Agilent, US) with nitrogen (2 ml/min ...
-
bioRxiv - Neuroscience 2024Quote: ... mut-shM4 (Table 5) and the QuikChange Multi Site-Directed Mutagenesis kit (200514; Agilent; Manufacturer Instructions) in several assays until all the mutations were incorporated ...
-
bioRxiv - Bioengineering 2024Quote: ... we employed a bright-field imaging using a Cytation 5 cell imaging multimode reader (Agilent Technologies). Images were stitched and z-projected prior to analysis of MFI and cancer cell area ...
-
bioRxiv - Neuroscience 2021Quote: ... anti-AIF-1/Iba1 (1:1000, 019741 DAKO); and secondary antibodies ...
-
bioRxiv - Pathology 2020Quote: ... and Ki-67 (1:100, MIB-1, Dako) in an Autostainer Link48 automated staining platform (Dako ...
-
bioRxiv - Developmental Biology 2020Quote: ... mouse anti-Ki67 (Dako, MIB-1, 1:100), mouse anti-DLX2 (Santa Cruz ...
-
bioRxiv - Immunology 2019Quote: ... RP1-5’-AAT GAT ACG GCG ACC ACC GAG ATC TAC ACG TTC AGA GTT CTA CAG TCC GA-3’ and RPI1-5’-CAA GCA GAA GAC GGC ATA CGA GAT CGT GAT GTG ACT GGA GTT CCT TGG CAC CCG AGA ATT CCA-3’ and the purified library was quantified with 4200 TapeStation (Agilent Technologies) and paired-end sequenced on a Nextseq 500 V2 (Illumina ...
-
bioRxiv - Genetics 2019Quote: ... The PAM sequences in the c(3)G gene were mutated using the Quik Change II XL Site-Directed Mutagenesis Kit (Agilent Technology). The bases changed are in bold above ...
-
bioRxiv - Bioengineering 2020Quote: ... Pelleted cells by the centrifugation for 3 min at 300 rcf are stained with FITC-conjugated anti-lysozyme antibody (Dako, F0372) and APC-conjugated anti-CD24 antibody (Biolegend ...
-
bioRxiv - Biochemistry 2020Quote: Measurements in both settings were performed in 3 min mix and 3 min measure cycles at 37 °C in six replicates per condition on a Seahorse XFe96 Analyzer (Agilent Technologies). OCR and ECAR were depicted as pmol/min and mpH/min ...
-
bioRxiv - Neuroscience 2021Quote: ... Samples were injected into a C18 reverse phase column (Atlantis T3, 2.1 × 150 mm, 3 µm; Waters) using HPLC (Agilent 1290 LC) at a flow rate of 0.15 ml/min with 5 mM ammonium formate for mobile phase A and 100% methanol for mobile phase B ...
-
bioRxiv - Immunology 2021Quote: ... HES5 and GATA6 genes were made for 3 replicates using the Brilliant III Ultra-Fast SYBR green qPCR master mix (Agilent Technologies) and analyzed on a CFX Opus Real-Time PCR system (BioRad ...
-
bioRxiv - Cell Biology 2021Quote: ... All immunostains were performed in Ventana Discovery (Ki-67 and cleaved caspase 3) or Dako autostainers using 3,3’ diaminobenzidine (DAB) chromogen (Dako-Agilent Technologies, Denmark). All slides were scanned using digital slide scanner NanoZoomer-XR C12000 (Hamamatsu ...
-
bioRxiv - Biophysics 2021Quote: ... then was injected to online SEC-SAXS equipped with a temperature-controlled (15 °C) silica-based SEC column (Agilent BioSEC-3)32 ...
-
bioRxiv - Cancer Biology 2021Quote: ... endogenous peroxidase activity was blocked by incubation with 3% H2O2 / Methanol for 10 minutes followed by incubation with Dako Dual Endogenous Enzyme Block reagent (Agilent Dako, S2003) for 30 min at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... heat mediated antigen retrieval was performed for 3 min at 125°C in citrate pH 6.0 target retrieval solution (Dako Cat# S2369) using a decloaking chamber (Biocare Medical) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Plates were read at 450 nm and 560 nm wavelengths using a BioTek Cytation 3 Cell Imaging Multi-Mode Microplate Reader (Agilent Technologies). Plasma samples isolated from retroorbital bleeds were used for ProcartaPlex immunoassays (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2021Quote: ... Scanning of microarrays was performed with 3 μm resolution (8×60K) using a G2565CA high-resolution laser microarray scanner (Agilent Technologies). Microarray image data were processed with the Image Analysis/Feature Extraction software G2567AA v ...
-
bioRxiv - Biochemistry 2021Quote: ... Samples were subsequently diluted to adjust the HNO3 concentration to 3 % and ICP-MS was performed with a Hewlett-Packard 4500 ICP mass spectrometer (Agilent Technologies) connected to a CETACASX-500 auto-sampler for sample injection ...
-
bioRxiv - Neuroscience 2022Quote: ... samples were injected into a C18 reverse phase column (Atlantis T3, 2.1 × 150 mm, 3 μm; Waters) using HPLC (Agilent 1290 LC) at a flow rate of 0.15□ml/min with 5 mM ammonium formate for mobile phase A and 100% methanol for mobile phase B ...
-
bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR was performed in technical triplicates from at least 3 independent biological samples using the SYBR green Brilliant II fast kit (Agilent Technologies) on an Mx3005p apparatus (Agilent Technologies ...
-
bioRxiv - Biochemistry 2023Quote: ... (3) RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
-
bioRxiv - Bioengineering 2023Quote: ... PHA was extracted from lyophilized sludge using acidified methanol (3% sulfuric acid) containing chloroform and analyzed using a gas chromatography-mass spectrometry (Agilent, USA). Glycogen in sludge was extracted in a similar way but using 0.9M HCl and analyzed using a liquid chromatography-mass spectrometer (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... DNA was cross-linked by exposing the living cells twice to 4,5′,8-trimethylpsoralen at a final concentration of 10 μg/mL followed by 3’ irradiation pulses with UV 365-nm monochromatic light (UV Stratalinker 1800, Agilent Technologies). The cells were then washed repeatedly with cold PBS and lysed with a cell lysis buffer (1.28M sucrose ...
-
bioRxiv - Biophysics 2023Quote: ... The monodisperse sample solutions of proteins were injected onto a size exclusion column (David and Perez 2009) (SEC-3, 150 Å; Agilent) using an Agilent HPLC system and eluted into the capillary cell at a flow rate of 0.3 ml min-1 ...
-
bioRxiv - Plant Biology 2023Quote: ... The samples were homogenized and the supernatant was loaded onto a Bond Elut C18 cartridge (100 mg, 3 ml; Agilent Technologies) and eluted with 80% (v/v ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The slides were then treated with 3% hydrogen peroxide for 10 min and then blocked with Serum-Free Protein Block (Dako, X0909) for 30 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... The pooled library was quality checked on an Agilent TapeStation System by mixing 3 µL D1000 Sample Buffer (Agilent 5190-6502) with 1 µL pooled library and running in D1000 ScreenTape (Agilent 5067-5582) ...
-
bioRxiv - Physiology 2023Quote: ... The metabolic function of activated CD4+ T cells cultured for 3 days in vitro was analyzed by measuring the extracellular acidification rate (ECAR) using an XFe96 extracellular flux analyzer (Seahorse Bioscience). The cells were kept in XF media (Seahorse Bioscience ...
-
bioRxiv - Cancer Biology 2023Quote: ... and absorbance was measured at 562 nanometers using the Bio-Tek Cytation 3 Multi-Mode Reader (Agilent, Santa Clara, CA, USA). The proteins were run on a 10% SDS-polyacrylamide gel ...
-
bioRxiv - Microbiology 2021Quote: ... Analytes were separated on a HP-5 capillary column (50m × 320 µm × 1.05 µm column, Agilent, USA). The carrier gas was helium (constant flow of 1.4 mL min-1) ...
-
bioRxiv - Genetics 2021Quote: ... The sections were rinsed in PBS/Tween buffer and treated with 5% Normal Goat Serum (Dako X0907) for 30 minutes after which they were incubated overnight at 4°C with a 1:25 dilution of F4/80 monoclonal rat anti-mouse antibody BM8 (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2019Quote: 3D-FISH was performed using the Dako FISH Histology accessory kit from Agilent (Catlog no: K579911-5) as per manufacturer guidelines ...
-
bioRxiv - Bioengineering 2021Quote: ... The GC-MS system used was the 7890-5975C from Agilent in combination with the HP-5MS column (5% phenyl methyl polysiloxane, 30m×0.25mm ID, 0.25µm) from Agilent. The temperature was maintained at 180 °C for 1 min ...
-
bioRxiv - Cancer Biology 2020Quote: ... Endogenous peroxidase activity was blocked for 5 min in using Dual Endogenous Enzyme Block (Cat. S2003, Dako). Primary Antibodies used included Ki67 (1:50 ...
-
bioRxiv - Biochemistry 2021Quote: ... on a ZORBAX SB-C18 Semi-preparative column (9.4 x 250 mm, 5 μm particle size; Agilent) and ZORBAX SB-C18 Analytical column (4.6 x 250 mm ...
-
bioRxiv - Plant Biology 2020Quote: ... equipped with a 5% phenyl-polymethylsiloxane capillary column (DB-5MS, 30 m × 0.25 mm × 1.0 μm; Agilent).
-
bioRxiv - Microbiology 2021Quote: ... and then subjected to semipreparative preparative HPLC (ZORBAX Eclipse XDB-C18, 9.4 × 250 mm, 5 μm, Agilent) using 0.1% formic acid in water/CH3OH as the mobile phase to afford pyridomycin B (25 mg).
-
bioRxiv - Microbiology 2021Quote: ... HPLC-MS analysis was performed on a ZORBAX RX-C18 column (150×4.6 mm, 5 μm, Agilent). The column was first equilibrated with 80% solvent A (0.1% formic acid in water ...
-
bioRxiv - Plant Biology 2021Quote: ... Filtered samples (5 μl each) were loaded onto a C18 high-capacity nano LC chip (Agilent Technologies) using a 1200 series capillary pump (Agilent Technologies ...
-
bioRxiv - Biochemistry 2020Quote: ... 5 μL were injected using an Agilent 1290 Infinity HPLC system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent Zorbax Eclipse Plus C18 column (2.1 × 150 mm ...
-
bioRxiv - Biochemistry 2021Quote: ... A semi-prep column (Zorbax 300SB-C18, 9.5 mm x 250 mm, 5 μm particle size, Agilent) was used for all purification steps ...
-
bioRxiv - Neuroscience 2020Quote: ... and fractionated using a ZORBAX 300Extend-C18 column (4.6 mm x 250 mm, 5 µm, Agilent Technologies) on an Agilent 1100 HPLC system monitored at 280 nm ...
-
bioRxiv - Microbiology 2020Quote: ... and Eclipse 5 μm column (9.4 × 250 mm) was used for the purification of marker compounds (Agilent). The chemicals and reagents used for standardisation and quality control were procured from Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2019Quote: ... with a Zorbax XDB C8 reverse phase column (4.6×150 mm, 5 μm, particle size, Agilent Technologies) was used for sample separate ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... and a Zorbax Eclipse Plus C18 guard column (4.6×12.5 mm, 5 µm Agilent, Santa Clara, CA) on an Agilent 1200 series HPLC attached to a UV based diode array detector at 260 nm (Agilent ...
-
bioRxiv - Systems Biology 2019Quote: ... and fractionated using a ZORBAX 300Extend-C18 column (4.6 mm x 250 mm, 5 µm, Agilent Technologies) on an Agilent 1100 HPLC system monitored at 280 nm ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 25 μl of each sample was injected through a Bio SEC-5 2000 Å HPLC column (Agilent) with a Bio SEC-5 2000 Å guard (Agilent) ...