Labshake search
Citations for Agilent :
2001 - 2050 of 2447 citations for 2' 4' Dichloro 3 2 5 dimethylphenyl propiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Temperature-dependent phase transition was detected by measuring the optical scattering at 800 nm while increasing the temperature from 4 to 60°C (with a ramping rate of 0.5°C/min) using a spectrophotometer Cary 300UV-VIS (Agilent Technologies). Nitrogen gas purging was used to prevent the moisture from condensing on the cuvette surface ...
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bioRxiv - Physiology 2024Quote: ... sections were washed three times with cold 0.1% Triton in PBS and then incubated with primary antibodies at 4 °C overnight after being blocked with protein blocking solution (DAKO) for 5-10 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Nine images were taken every 4 hours in each well and the number of cells by images were determined using BioTek Gen5 Software (Agilent).
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bioRxiv - Immunology 2024Quote: 0.4 ug of biotinylated SL9 (SLYNTAVTL) or IV9 (ILKEPVHGV) peptides (NIH Tetramer Core Facility) were conjugated to PE-streptavidin (Agilent), APC-streptavidin (Agilent ...
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bioRxiv - Synthetic Biology 2024Quote: ... GFP fluorescence was measured every 15 min for up to 4 h and every 30 min thereafter (Mx3005P; Agilent Technologies). The slope of the GFP fluorescence from 0 to 4 h was evaluated for each aaRS activity ...
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bioRxiv - Synthetic Biology 2024Quote: ... GFP fluorescence was measured every 15 min for up to 4 h and every 30 min thereafter (Mx3005P; Agilent Technologies). The slope of the GFP fluorescence from 0 to 4 h was evaluated as the GFP synthesis rate ...
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bioRxiv - Bioengineering 2024Quote: ... we washed and cultured BMDMs in the Seahorse XF DMEM medium (pH 7.4) supplemented with 25 mM D-glucose and 4 mM Glutamine (all sourced from Agilent Technologies) for an hour in a non-CO2 incubator at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Images were automatically taken every 4 hours over 72 hours in a BioTek BioSpa Automated Incubator (Agilent, Santa Clara, CA). Cell proliferation was quantified using Aligent’s Gen5 image analysis tools.
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bioRxiv - Plant Biology 2020Quote: ... The sample components were separated on a C18 olumn (Hypersil GOLD C18; 4.6 mm × 100 mm; 3 µm particle size; Agilent, Santa Cruz, CA, USA). The column was eluted at 0.3 mL/min with a gradient from 95% solvent A (0.1% formic acid in water ...
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bioRxiv - Developmental Biology 2022Quote: ... High quality total RNA (RNA integrity number = 10) was labelled with Cyanine 3 CTP using the Low Input Quick Amp Labelling Kit (Agilent Technologies-5190-2305), and purified using Qiagen’s RNeasy Mini Spin Columns ...
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bioRxiv - Biochemistry 2022Quote: ... All libraries at this step yielded >300,000 colonies implying a 100x coverage (assuming 1/3 of the variants were perfect based on our previous analysis of Agilent OLS based libraries). Each sublibrary was combined at an equimolar ratio to make a complete library with all intended mutations included.
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bioRxiv - Microbiology 2021Quote: ... The extracts were acidified to pH 2 and additionally pre-treated with solid-phase extractions using Agilent Bond Elut-PPL 3 mL columns and diluted to 50 ppm (Agilent Technologies, DE, USA) following standard lab protocol29 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Primary antibodies were diluted 1:4000 for anti-sGCα1 and 1:2000 for anti-sGCβ1 antibody in 3% dry milk in TBST and incubated with nitrocellulose membranes at 4°C over-night following challenge of membranes with secondary goat anti-rabbit antibody (1:2000 in 3% milk in TBST) conjugated to horseradish peroxidase (Dako A/S, Denmark). Immuno-complexes were visualized using an enhanced chemiluminescence kit (Amersham Pharmacia Biotech ...
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bioRxiv - Biochemistry 2022Quote: 50 μL of pure CrRPE1 concentrated at 9.5 mg mL−1 were injected on BioSEC-3 300 size-exclusion chromatography column (Agilent Technologies, Santa Clara, USA) equilibrated in buffer 20 mM Tris-HCl (pH 7.9 ...
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bioRxiv - Biophysics 2022Quote: ... The samples were then further diluted in ultrapure water to a final 3% nitric acid concentration and analyzed by triple quadrupole inductively coupled plasma-mass spectrometry (Agilent 8800 ICP-QQQ). Copper concentrations were determined by using standard curves ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- ACC TCC ACC ACC AGA ACC ACC TCC ACC GCT CTG AAA GTA CAG ATC CTC GGT ACC TTT ATA TAA TTC GTC CAT-3’) using the Agilent QuikChange SDM kit following the manufacturer’s instructions (Agilent, Singapore, Singapore; Cat: STR_210519). This was followed by amplifying the backbone of the plasmid using primer set Ag43BBGib_F ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- CTT TTT CAA ATT GGG GGT GTG ACC ATG CAG CGC TAG CCG CAG C −3’) using the GeneMorph II epPCR kit (Agilent, Singapore, Cat: STR_200550). The epPCR profile is as follows ...
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Age, sex, and cell type-resolved hypothalamic gene expression across the pubertal transition in micebioRxiv - Genomics 2023Quote: RNA-seq libraries were prepared using an automated - QuantSeq 3’mRNA-seq (Lexogen GmbH, Vienna) and Agilent NGS Workstation (Agilent Technologies, Santa Clara) at The Centre for Applied Genomics (TCAG ...
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bioRxiv - Plant Biology 2024Quote: ... For separation of all metabolites, we used a Zorbax RRHD Eclipse XDB-C18 column (50 x 3 mm, 1.8 µm) (Agilent, Santa Clara, CA, USA) with a 1290 Infinity II inline filter (0.3 µm ...
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bioRxiv - Immunology 2024Quote: ... The antigens on the slides were retrieved by submerging slides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent, Cat# S2375) and incubating at 97 °C for 40 min in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4-µm sections were cut and antigen retrieval was carried out by heat treatment with Target Retrieval Solution (S1699, Agilent Technologies) for Ki67 ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then incubated overnight at 4°C with primary polyclonal anti-GFAP rabbit antibody (1:1000; Z0334, DAKO, Golstrup, Denmark) or anti-collagen IV rabbit antibody (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... dilution 1:100)] in TNB blocking buffer for overnight at 4°C followed by one-hour incubation with secondary antibodies: EnVisionTM+ System-HRP (DAKO K4002). TRITC-based Tyramide reagent pack from Perkin Elmer was used to amplify the fluorescence of Ki67 and c-Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Sample pH was then adjusted to ~4 with formic acid prior to loading on Bond Elut-PH SPE columns (Agilent, 14102005), washing with aqueous buffer (30 mM ascorbic acid ...
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bioRxiv - Molecular Biology 2021Quote: ... The sections were incubated with a primary antibody at 4°C overnight and with a rabbit/mouse HRP-conjugated secondary antibody (Dako, Denmark) at room temperature for 2 h ...
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bioRxiv - Neuroscience 2022Quote: ... Plasma (50 μL) was diluted by adding 150 μL of Agilent buffer A (1:4 dilutions, as recommended by Agilent Technologies), then filtered using a 0.45μm spin filter (Spin-X centrifuge tube filter ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated overnight at +4 °C with primary antibodies diluted in the same solution or in an antibody diluent (Agilent #S3022). The samples were then washed in 0.1 % Triton X-100 or 1.0 % Tween20 in PBS for 30-60 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Protein immunodetection was performed with primary abs diluted in blocking solution during overnight incubation at 4°C: rabbit polyclonal ab against alpha-1-antitrypsin (A0012, Dako, Agilent) at a 1:500 dilution ...
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bioRxiv - Biochemistry 2020Quote: ... SDM was carried out with appropriate primers (Table 4) using QuikChange® Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) according to manufacturer’s instruction.
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bioRxiv - Cell Biology 2021Quote: Primary SVF cells from BAT and sWAT were isolated and cultured for 4 days before being plated in XF cell culture microplates (Seahorse Bioscience). SVF cells (10,000 cells ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed with 4 μl Brilliant III Ultra-Fast SYBR QPCR as instructed by the manufacturer (Agilent Santa Clara). All primers for quantification of either expression of viral m/cRNAs or antiviral responses are shown below.
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bioRxiv - Genetics 2022Quote: ... and incubated in the dark overnight at 4 C before running on a Novocyte Quanteon flow cytometer using NovoExpress 1.3.0 software (Agilent, Santa Clara, CA) at a flow rate of 14 uL/minute ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated overnight at 4°C and appropriate HRP-conjugated secondary antibodies (DAKO, Agilent Technologies, Santa Clara, CA, USA) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... The Samples were diluted 4 fold with water and 1 μl supernatant was loaded on a Bioanalyzer High Sensitivity DNA electrophoresis chip (Agilent Technologies).
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bioRxiv - Zoology 2020Quote: ... hydrated through graded ethanol and incubated overnight at 4°C with a mouse anti-human CD68 clone KP1 monoclonal antibody (1:100, Dako, Denmark). Then ...
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bioRxiv - Biochemistry 2021Quote: ... or PDK1SKD-PIF S241A (4 mg/ml) was injected onto a Superdex 200 10/300 column (Cytiva) operated by a 1260 Infinity HPLC (Agilent Technologies). Light scattering of a 690 nm laser was detected by a MiniDawn Treos (Wyatt ...
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bioRxiv - Developmental Biology 2021Quote: ... for 6 minutes (30 seconds ON, 30 seconds OFF) at 4°C to obtain ~200 bp fragments (confirmed using an Agilent Bioanalyzer). Sonicated DNA was extracted using ethanol precipitation ...
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bioRxiv - Cell Biology 2020Quote: ... All hESC lines were analysed for their genetic content by array-based comparative genomic hybridization (Human Genome CGH Microarray 4×44K, Agilent Technologies) as previously described (Jacobs et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were fixed for 15 min in 4% paraformaldehyde and blocked for 60 min in blocking solution (DAKO, Mississauga, Ontario, Canada). Sections were incubated overnight in polyclonal rabbit anti-GFAP primary antibody and monoclonal mouse anti-tau antibody (DC190) ...
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bioRxiv - Cancer Biology 2022Quote: Genome-wide copy number variations were assessed using the SurePrint G3 Human aCGH 4×180K Microarray (Agilent Technologies, Santa Clara, CA), according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... sections were post-fixed with 4% formaldehyde for 10 minutes and histologically stained with Dako Mayers hematoxylin (Agilent, cat. no. S3309) (4 minutes ...
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bioRxiv - Immunology 2024Quote: A total of 10 µg/ml rHA were coated on an ELISA plate at 4 °C ON and afterwards incubated with rabbit anti-HA antibody (1:2000 dilution) (Dako, A0001) 2h at RT ...
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bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sample pH was then adjusted to 4 with 15 μL of 20% formic acid before loading onto conditioned SPE Bond-Elute pH columns (Agilent, 14102062) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... solution (pH 7.4) and incubated with goat anti-mouse or anti-rabbit immunoglobulin labeled with dextran molecules and horseradish peroxidase (EnVision, Dako Omnis) at room temperature for 30 min ...
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bioRxiv - Genomics 2023Quote: Each purified GST-tagged DBD stock was validated for DNA-binding specificity using 4×44k universal protein-binding microarrays (PBMs) (Agilent Technologies), as described previously42 ...
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bioRxiv - Bioengineering 2023Quote: ... equipped with a UV detector through a C18 column Poroshell 120 EC-C18 (4.6 × 150 mm, 4 μm) (Agilent Technologies, Inc.). Mobile phase consisted of acetonitrile/methanol/0.4% acetic acid/ tetrahydrofuran (80:12:5:3 ...
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bioRxiv - Cancer Biology 2023Quote: The 4-6um thick TMA sections were deparaffinized and antigens were unmasked using EnVision FLEX Target Retrival Solution Low pH (Dako Agilent) in PT link device (PT200 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were fixed for 15 min in 4% paraformaldehyde and blocked for 60 min in a blocking solution (DAKO, Ontario, Canada). Sections were incubated overnight in a polyclonal rabbit anti-IBA-1 primary antibody (1:1000 ...
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bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...