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Citations for Agilent :
151 - 200 of 2486 citations for Small ribosomal subunit protein uS12 RPS23 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and tRNA was isolated to purity from the small RNA fraction following HPLC on the Bio SEC-3 column (Agilent; 7.8 mm ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA quality and correspondence with sample characteristics were evaluated on a subset of the samples by migrating a small amount of DNA on a TapeStation 4200 (Agilent) to calculate the DNA Integrity number (DIN) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then intracellularly stained with 4G2 mAbs followed by rabbit anti-mouse Igs FITC (Dako). The cells were fixed with 1% formaldehyde and analyzed on BD LSRFortessa ...
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bioRxiv - Neuroscience 2022Quote: ... Protein blocking was performed using protein block solution (DAKO) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were incubated overnight with the following primary antibodies: rabbit polyclonal anti-glial fibrillary acidic protein (GFAP) (1:6,000, DAKO, UK), rabbit polyclonal anti-allograft inflammatory factor 1 (IBA1 ...
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bioRxiv - Bioengineering 2022Quote: ... and incubated overnight at 4 °C with primary antibodies for the glial fibrillary acidic protein (GFAP; 1:1000, Z0334, Dako), ionised calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Physiology 2024Quote: ... sections were washed three times with cold 0.1% Triton in PBS and then incubated with primary antibodies at 4 °C overnight after being blocked with protein blocking solution (DAKO) for 5-10 min at room temperature ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The entire sample was then added to a 250 µL small volume pulled point class insert (Agilent Part No: 5183-2085) to facilitate autosampler needle access ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA concentration was then quantified at The Center for Functional Genomics/University at Albany using an Agilent Technologies 2100 Bioanalyzer System (Santa Clara, CA) and respective Bioanalyzer Small RNA reagents (catalog no. 5067-1548, Agilent Technologies). Purified small RNA samples were then digested using Nucleoside Digestion Mix (catalog no ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA and small RNA from BH were analyzed using capillary electrophoresis by RNA 6000 Nano Kit (Agilent Technologies 5067-1511) and RNA 6000 Pico Kit.
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bioRxiv - Biochemistry 2022Quote: ... Point mutations and small deletions or insertions were generated using QuickChange [80] with Turbo Pfu DNA polymerase (Stratagene, La Jolla, CA). A nuclear localization signal (NLS) ...
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bioRxiv - Molecular Biology 2021Quote: ... and the binding of small fragments (35-2000 bp) using a 2100 Bioanalyzer with an Agilent high-sensitivity DNA chip (Agilent Technologies).
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bioRxiv - Immunology 2023Quote: ... according to manufacturer’s instructions and RNA concentrations were measured with the Agilent 2100 bioanalyzer using a Small RNA Chip (Agilent 5067-1548).
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was washed with 80% ethanol and sizes of generated RNA fragments were assessed by capillary electrophoresis using a small RNA chip on Bioanalyzer 2100 (Agilent, USA). RNA fragments were directly 3’-end dephosphorylated using 5 U of Antarctic Phosphatase (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: Small RNA and miRNA RNA quantity and quality in the nine EV samples were assessed by capillary electrophoresis in the 2100 Bioanalyzer and the Agilent Small RNA kit (Agilent Technologies). miRNA libraries were generated with the NEXTFLEX Small RNA Sequencing Kit v3 (©Bioo Scientific Corp. ...
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bioRxiv - Bioengineering 2023Quote: ... protein block with Dako protein block solution (Dako, Glostrup, Denmark), incubation with primary antibodies [eGFP (Abcam ...
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bioRxiv - Immunology 2021Quote: ... Protein Block (Agilent) was used to block nonspecific antibody binding before incubating the sections with primary antibody overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound antibody was removed with washing buffer and the fraction of cells with surface protein labeled with CD44 antibody was determined using a MoFlo cell sorter (Dako Cytomation).
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies were directed against: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), epithelial membrane antigen (EMA ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies were directed against: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), epithelial membrane antigen (EMA ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatants from 1mL transient transfections of the antibodies were purified using the AssayMAP BRAVO platform with 5mL Protein A cartridges (Agilent Technologies). Lager scale transient transfections were purified on the Akta FPLC system using 1mL HiTrap MabSelect PrismA™ affinity chromatography resin (Cytiva) ...
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bioRxiv - Neuroscience 2024Quote: Mouse hind paws were fixed in Zamboni’s fixative for 24-48 hours at 4°C and processed as previously described.44 50 μm floating tissue sections were stained with the primary antibody rabbit anti-protein-gene-product 9.5 (PGP 9.5) (Dako, Z5116, 1:500) overnight and the secondary biotinylated goat anti-rabbit IgG antibody (Vector Laboratories ...
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bioRxiv - Pathology 2024Quote: ... sections were incubated overnight at 4 ⁰C with a cocktail of the primary antibodies αSyn (clone KM51 1:500, Monosan Xtra, The Netherlands) and glial fibrillary acidic protein (GFAP, Z0334, 1:4000, DAKO, Denmark). After rinsing in PBS ...
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bioRxiv - Neuroscience 2020Quote: All MRI experimental measurements were performed with an Agilent 7T (300 MHz) horizontal bore small animal MRI (Agilent Inc.; Santa Clara, California) with an open bore of 310 mm and a diameter of 115 mm inside the gradient coil (Resonance Research Inc. ...
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bioRxiv - Microbiology 2023Quote: ... yield and size of total and small RNAs were analysed using capillary electrophoresis (Agilent 2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA). The TruSeq Small RNA-seq preparation kit (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... A comprehensive database comprising 850 small molecules was created using the Agilent Personal Compound Database and Library (PCDL) software (Agilent technologies, USA). The software is used to manage the content of personal compound databases and libraries and is part of the Agilent MassHunter Workstation software suite ...
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bioRxiv - Immunology 2020Quote: ... FFU/ml) using an anti-NP MAb (HB-65) and a FITC-conjugated anti-mouse secondary Ab (Dako). Geometric mean titers and data representation were performed using GraphPad Prism ...
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bioRxiv - Microbiology 2023Quote: ... rabbit F(ab’)2 anti-human C1q-FITC (both at 5 µg/ml, Dako as described in (9)) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... plasma FITC concentration was measured as fluorescence intensity using BioTek Synergy H1 (Agilent Technologies Japan, Ltd., Tokyo, Japan).
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bioRxiv - Neuroscience 2021Quote: ... the co-cultures were incubated first with primary antibody for glial fibrillary acidic protein (GFAP, 1:400, Z033429-2, Dako, Glostrup, Denmark) prepared in 5% NGS in DPBS overnight at +4 following an incubation with Alexa Fluor405 (A31556 ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Molecular Biology 2020Quote: ... The size distribution and concentration of s-mEV miRNA was further assessed using a 2100 Agilent Bioanalyzer with an Agilent Small RNA Kit (Agilent Technologies, CA, USA), according to the manufacturers’ instruction.
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bioRxiv - Neuroscience 2023Quote: ... The yield and size distribution of the small RNA libraries were assessed using the Fragment Bioanalyzer™ system with DNA 1000 chip (Agilent 5067-1505). After size selection of the libraries by agarose gel cassettes (Sage Science HTG3010 ...
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bioRxiv - Molecular Biology 2024Quote: ... And assessed for their size distribution and adapter dimer presence using the High Sensitivity Small Fragment DNF-477 Kit (Agilent, # DNF-477-0500) on a 48-channel Fragment Analyzer (Agilent ...
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bioRxiv - Immunology 2020Quote: ... protein block (Dako X0909) for 20 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: ... and Protein block (Dako) before staining with anti-pS6 (detected with fluorochrome conjugated secondary antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... and protein block (DAKO) were conducted before a 1-hour primary antibody incubation at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then incubated for 1 hour at room temperature with rabbit anti-glial fibrillary acidic protein (GFAP; rabbit anti-GFAP antibody, 1:300, Dako, Z0334, Carpinteria, CA), rabbit anti-oligodendrocyte specific protein (OSP ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The slides were processed as described and probed with a set of 258 antibodies against total proteins and phosphoproteins using an automated slide stainer Autolink 48 (Dako, Santa Clara, CA). Each slide was incubated with one specific primary antibody and a negative control slide was incubated with antibody diluent without any primary antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were processed as described and probed with a set of 264 antibodies against total proteins and phosphoproteins using an automated slide stainer Autolink 48 (Dako, Santa Clara, CA). Each slide was incubated with one specific primary antibody and a negative control slide was incubated with antibody diluent without any primary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated for 1h at RT before overnight incubation at 4°C with primary antibodies: Glial Fibrillary Acidic Protein (GFAP) (1/500 Dako Cat. Number Z0334); AQP4 (1/500 ...
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bioRxiv - Neuroscience 2020Quote: ... The yield and size distribution of the small RNA libraries were assessed using the Agilent 2100 Bioanalyzer™ instrument with DNA 1000 chip (Agilent Technologies 5067-1504). Libraries were prepared for deep sequencing using the Ion Chef system (Life Technologies 4484177 ...
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bioRxiv - Microbiology 2020Quote: FITC-conjugated rabbit F(ab’)2 anti-human C1q was obtained by digestion of FITC-conjugated rabbit anti-human C1q (Dako) using 1 U/ µg of recombinant His-tagged IdeS protease ...
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bioRxiv - Cancer Biology 2020Quote: ... mononuclear cells from BM of non-metastasized breast cancer patients were subjected to immunofluorescent staining for EpCAM (Ber-EP4-FITC, Agilent or HEA-125-PE ...
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bioRxiv - Cancer Biology 2020Quote: ... mononuclear cells were screened accordingly to the method for human DCCs using immunofluorescent staining of the cell suspension with anti-human EpCAM (Ber-EP4-FITC, Agilent, or HEA-125-PE ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with PBS and incubated with a fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG (Dako; 1:200). Cell nuclei were stained with 4’ ...
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bioRxiv - Developmental Biology 2022Quote: ... the sections were washed twice in TTBS buffer before incubation in a diluted solution of Fluorescein (FITC) conjugated F (ab’)₂ fragment goat anti-rabbit IgG (Dako) or Alexa Fluor 647 conjugated F(ab)2 fragment swine anti-rabbit secondary antibody (Molecular Probes) ...
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bioRxiv - Biochemistry 2024Quote: ... Fluorescence anisotropy titrations with FITC-labeled Nbs and CtRoco were performed at 25°C using a Cary Eclipse spectrofluorometer (Agilent) equipped with polarizers and temperature control ...
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bioRxiv - Microbiology 2024Quote: ... The FITC concentration in the serum was quantified fluorometrically using a multimode reader (Synergy H1, Agilent technologies, Santa Clara, CA) at an emission wavelength of 528 nm and an excitation wavelength of 485 nm ...