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Citations for Agilent :
151 - 200 of 683 citations for Recombinant Human FAS His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Physiology 2020Quote: ... 20 μL samples were injected into Hi-Plex H column (300×7.7 mm; 8 μm particle size, Agilent Tech.). 0.1% formic acid in Milli-Q water (Merck Millipore ...
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bioRxiv - Microbiology 2019Quote: ... The RNA was DNase treated using Turbo DNA free kit according to manufacturer’s protocol (Thermo Fischer scientific) for making cDNA using Accuscript hi-fidelity cDNA synthesis kit (Agilent). The qRT-PCR was set up using brilliant III ultra-fast SYBRgreen qPCR master mix in Mx3005P qPCR system Agilent ...
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bioRxiv - Genetics 2020Quote: ... Synthesis of cDNA was carried out with the AccuScript Hi-Fi cDNA Synthesis Kit (Agilent Technologies, Santa Clara, USA) using Oligo(dT ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification of pcDNA3.1-NS3-mCherry-HIS plasmid was done by using PfuUltra HotStart DNA Polymerase (Agilent, Cat #: 600390) and forward and reverse primers (table 2 ...
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bioRxiv - Microbiology 2020Quote: ... biotinylated recombinant TRAP proteins (Table 1) were oligomerized around a streptavidin-conjugated Phycoerythrin (PE) (PJRS2, Prozyme) creating TRAP-PE staining complexes ...
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bioRxiv - Neuroscience 2020Quote: ... the recombinant adenovirus vector was produced using AdEasy (Ad) Vector System (Stratagene, La Jolla, CA, USA). The infection controls were Ad-GFP.
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bioRxiv - Neuroscience 2019Quote: ... Recombinant proteins were expressed in BL21-CodonPlus (DE3)-RILP Competent Cells (Agilent Technologies, Santa Clara, CA) and purified using Glutathione Sepharose 4B Media (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... All recombinant proteins were expressed in the BL21-Gold (DE3) pLysS strain of Escherichia coli (Agilent) in pGEX-KG vectors as glutathione S-transferase (GST ...
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bioRxiv - Microbiology 2020Quote: FITC-conjugated rabbit F(ab’)2 anti-human C1q was obtained by digestion of FITC-conjugated rabbit anti-human C1q (Dako) using 1 U/ µg of recombinant His-tagged IdeS protease ...
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bioRxiv - Cancer Biology 2020Quote: ... mononuclear cells were screened accordingly to the method for human DCCs using immunofluorescent staining of the cell suspension with anti-human EpCAM (Ber-EP4-FITC, Agilent, or HEA-125-PE ...
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bioRxiv - Genomics 2020Quote: The Universal Human Reference RNA was from Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... rabbit antibody against anti-human MPO (A0398, Dako), mouse β actin (A1978 ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies against human tau (DAKO Cat# A0024) and β-actin (Cell Signaling Cat# 3700 ...
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bioRxiv - Immunology 2020Quote: ... mouse-anti-human EGFR (Dako, DAK-H1-WT), and CLTX-biotin ...
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bioRxiv - Genomics 2019Quote: ... Library construction (Agilent SureSelect Human All Exon kit), quality assessment ...
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bioRxiv - Genomics 2019Quote: ... SureSelectXT Human all exon V6 kit (Agilent Technologies) was used to capture exons ...
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bioRxiv - Neuroscience 2020Quote: ... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
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bioRxiv - Neuroscience 2022Quote: ... Rabbit anti–human vWF (A0082, Agilent, 1:300). Primary antibodies were detected with the corresponding Alexa Fluor -555 ...
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bioRxiv - Immunology 2022Quote: ... and mouse anti-human CD68 (Dako, M087601-2) and rabbit anti-human PD-L1 (Ventana ...
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bioRxiv - Bioengineering 2022Quote: ... human CD31 (mouse, 1:200, clone JC70A, Agilent), mouse CD31 (rat ...
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bioRxiv - Cancer Biology 2022Quote: ... IHC for human Ki67 (IR62661-2, Agilent Technologies) was used to ensure the propagated tumors were comprised of human cells ...
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bioRxiv - Cancer Biology 2020Quote: ... Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis ...
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bioRxiv - Genomics 2019Quote: ... Universal Human Reference RNA was purchased from Agilent Technologies (#740000 ...
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bioRxiv - Biophysics 2019Quote: The human embryonic kidney AD-293 cells (Agilent) were cultured in growth medium that consisted of Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cancer Biology 2021Quote: ... A SureSelectXT Human All Exon V6 kit (Agilent) was used for exome capture ...
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bioRxiv - Immunology 2022Quote: ... A whole Human Genome Microarray 4X44K (Agilent Technologies) was used for gene expression analysis ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... human vimentin (1:4,000; M0725; DakoCytomation; Glostrup, Denmark), P-gp (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... Rabbit anti-human GFAP (1:400, Dako, Z0334), Rabbit anti-human SLC1A3 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... rabbit polyclonal anti-Human/mouse vWf (Dako #A0082), rat monoclonal anti-mouse/rat FOXP3 (clone FJK-16s ...
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bioRxiv - Cancer Biology 2024Quote: ... libraries were prepared using SureSelect XT Human (Agilent) and captured using a customized panel covering exonic regions of 435 genes ...
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bioRxiv - Immunology 2024Quote: ... or rabbit anti-human C3d polyclonal Ab (Agilent) primary antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... pcDNA3.1-HA-SorCS1cβ Y1132A and GFP-tagged Rab7 T22N were generated by in vitro mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent, Cat #200522) from pcDNA4-SorCS1cβ-myc (kindly provided by Alan D ...
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bioRxiv - Cell Biology 2021Quote: ... The substitution S59D and S59A were introduced into the pLVX-IRES-FLAG-tagged HERPUD1 vector using the QuikChange II XL direct-mutagenesis kit (Stratagene, cat#200522) and the mutagenesis service of GenScript (Hong Kong ...
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bioRxiv - Microbiology 2023Quote: Plasmids encoding hexahistidine (Hisx6)- and glutathione S-transferase (GST)-tagged proteins were introduced into Escherichia coli strain BL21(DE3) CondonPlus RIL (230245, Agilent Technologies, Inc.), and the expression of the recombinant protein was induced by 0.5 mM isopropylthio-β-D-galactoside (IPTG ...
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bioRxiv - Cancer Biology 2019Quote: Microarray experiments were performed as dual-color or single-color hybridizations on either Agilent Whole Human Genome 4×44K microarrays (Design ID 014850) or 8×60K human custom (Agilent-048908) microarrays comprising identical features for coding genes ...
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bioRxiv - Genomics 2022Quote: ... Each pool was enriched twice with the SureSelect Human All Exon v7 probes and the latest version of the probes SureSelect Human All Exon v8 (Agilent Technologies) for the second time ...
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bioRxiv - Molecular Biology 2023Quote: ... The reporter cell lines were stained with anti-human CD45 and anti-human CD19 antibodies and analyzed by flow cytometry on an ACEA NovoCyte 2060 (Agilent, USA). ACEA NovoExpress (Agilent ...
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bioRxiv - Biophysics 2021Quote: ... The plasmid encoding the protein fused with a His-tag at the N-terminus was transformed in BL21 DE3 pLysS strains (Agilent). Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich ...
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bioRxiv - Systems Biology 2021Quote: ... respectively) on a 300 × 7.7 mm PL Hi-Plex H+ (8 µm particle size) column (Agilent Technologies, Santa Clara, CA) and 4 x 3 mm ID Carbo-H guard (Phenomenex ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A 20 µl sample was injected into the Hi-Plex H column (300×7.7 mm; 8 µm particle size, Agilent Tech.). 0.1% formic acid in Milli-Q water (Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: The CAN97-83 HMPV P construct was cloned into the plasmid pET 302/NT-His between the cleavage sites EcoRI and XhoI and expressed in BL21(DE3) CodonPlus RIL cells (Agilent) overnight at 37 °C in terrific broth (TB ...
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bioRxiv - Cell Biology 2021Quote: ... W224A mutants were obtained in the pcDNA5 His PC TEV Arpin plasmid using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent). ORFs encoding Arpin WT and mutant forms were subcloned between FseI and AscI sites into custom-made pcDNAm FRT PC GFP ...
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bioRxiv - Cell Biology 2021Quote: ... The three missense mutations identified were introduced into a previously described pcDNA3.1-CD33-V5/HIS vector using a QuikChange XL kit (Agilent 200517) with forward primer 5’-GCACTTGCAGCCGGATTTTTGGATCCATAGCCAGGGCC and reverse primer 5’-GGCCCTGGCTATGGATCCAAAAATCCGGCTGCAAGTGC (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: Site directed mutagenesis was performed on pGAPZα-His-MspGH25 vector according to the instructions of the QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Genetics 2022Quote: ... the other mtr4 mutant plasmids were constructed by site-directed mutagenesis of the MTR4 HIS CEN6 plasmid (pAC4096) with the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and oligonucleotides containing the corresponding missense mutations ...
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bioRxiv - Biochemistry 2023Quote: ... R448A and N455A were introduced in the pETNKI-his-JBP1 JDBD plasmid using a modified QuickChange site-directed mutagenesis method (Agilent). Forward and reverse mutagenesis primers containing the single point mutations were purchased from ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... 500-1000ng of Hi-C library were captured using the SureSelectXT Target Enrichment System for the Illumina Platform (Agilent Technologies) as instructed by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant adenovirus particles were produced following the AdEasy XL Adenoviral Vector System protocol (#240010 Agilent Technologies). Islets were transduced using a microfluidic device to obtain uniform infection of the islet cells throughout the islet volume and were incubated overnight before imaging ...
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bioRxiv - Genomics 2019Quote: Same amounts of early and late-labeled DNA were loaded on mouse or human DNA microarrays (SurePrint G3 Human CGH arrays, Agilent Technologies, G4449A). Hybridization was performed as previously described (Hadjadj et al. ...