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Citations for Agilent :
151 - 200 of 669 citations for Recombinant Human CEACAM1 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... or rabbit anti-human C3d polyclonal Ab (Agilent) primary antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... libraries were prepared using SureSelect XT Human (Agilent) and captured using a customized panel covering exonic regions of 435 genes ...
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bioRxiv - Immunology 2022Quote: ... A whole Human Genome Microarray 4X44K (Agilent Technologies) was used for gene expression analysis ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... human vimentin (1:4,000; M0725; DakoCytomation; Glostrup, Denmark), P-gp (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... human anti-CD34 antibody (clone QBend-10, DAKO), human anti-CD14 antibody (clone 7 ...
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bioRxiv - Molecular Biology 2024Quote: ... rabbit anti human vWF (1:200, Dako GA527) and goat anti human FH (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... The substitution S59D and S59A were introduced into the pLVX-IRES-FLAG-tagged HERPUD1 vector using the QuikChange II XL direct-mutagenesis kit (Stratagene, cat#200522) and the mutagenesis service of GenScript (Hong Kong ...
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bioRxiv - Microbiology 2023Quote: Plasmids encoding hexahistidine (Hisx6)- and glutathione S-transferase (GST)-tagged proteins were introduced into Escherichia coli strain BL21(DE3) CondonPlus RIL (230245, Agilent Technologies, Inc.), and the expression of the recombinant protein was induced by 0.5 mM isopropylthio-β-D-galactoside (IPTG ...
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bioRxiv - Biophysics 2021Quote: ... The plasmid encoding the protein fused with a His-tag at the N-terminus was transformed in BL21 DE3 pLysS strains (Agilent). Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich ...
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bioRxiv - Systems Biology 2021Quote: ... respectively) on a 300 × 7.7 mm PL Hi-Plex H+ (8 µm particle size) column (Agilent Technologies, Santa Clara, CA) and 4 x 3 mm ID Carbo-H guard (Phenomenex ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A 20 µl sample was injected into the Hi-Plex H column (300×7.7 mm; 8 µm particle size, Agilent Tech.). 0.1% formic acid in Milli-Q water (Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: The CAN97-83 HMPV P construct was cloned into the plasmid pET 302/NT-His between the cleavage sites EcoRI and XhoI and expressed in BL21(DE3) CodonPlus RIL cells (Agilent) overnight at 37 °C in terrific broth (TB ...
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bioRxiv - Cell Biology 2021Quote: ... W224A mutants were obtained in the pcDNA5 His PC TEV Arpin plasmid using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent). ORFs encoding Arpin WT and mutant forms were subcloned between FseI and AscI sites into custom-made pcDNAm FRT PC GFP ...
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bioRxiv - Cell Biology 2021Quote: ... The three missense mutations identified were introduced into a previously described pcDNA3.1-CD33-V5/HIS vector using a QuikChange XL kit (Agilent 200517) with forward primer 5’-GCACTTGCAGCCGGATTTTTGGATCCATAGCCAGGGCC and reverse primer 5’-GGCCCTGGCTATGGATCCAAAAATCCGGCTGCAAGTGC (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: Site directed mutagenesis was performed on pGAPZα-His-MspGH25 vector according to the instructions of the QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Genomics 2023Quote: ... 500-1000ng of Hi-C library were captured using the SureSelectXT Target Enrichment System for the Illumina Platform (Agilent Technologies) as instructed by the manufacturer ...
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bioRxiv - Genetics 2022Quote: ... the other mtr4 mutant plasmids were constructed by site-directed mutagenesis of the MTR4 HIS CEN6 plasmid (pAC4096) with the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and oligonucleotides containing the corresponding missense mutations ...
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bioRxiv - Biochemistry 2023Quote: ... R448A and N455A were introduced in the pETNKI-his-JBP1 JDBD plasmid using a modified QuickChange site-directed mutagenesis method (Agilent). Forward and reverse mutagenesis primers containing the single point mutations were purchased from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... human cGAS was expressed as a fusion protein with a 6×His-SUMO2 tag in Escherichia coli (E. coli) BL21-RIL DE3 (Agilent) bacteria harboring a pRARE2 tRNA plasmid ...
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bioRxiv - Developmental Biology 2024Quote: ... 1ug of Hi-C library per sample were hybridized with 120 nucleotide biotinylated RNA probes using the SureSelect kit from Agilent and PCR amplification using the polymerase from the Kapa Hyper Prep Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... The reporter cell lines were stained with anti-human CD45 and anti-human CD19 antibodies and analyzed by flow cytometry on an ACEA NovoCyte 2060 (Agilent, USA). ACEA NovoExpress (Agilent ...
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bioRxiv - Genomics 2022Quote: ... Each pool was enriched twice with the SureSelect Human All Exon v7 probes and the latest version of the probes SureSelect Human All Exon v8 (Agilent Technologies) for the second time ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant adenovirus particles were produced following the AdEasy XL Adenoviral Vector System protocol (#240010 Agilent Technologies). Islets were transduced using a microfluidic device to obtain uniform infection of the islet cells throughout the islet volume and were incubated overnight before imaging ...
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bioRxiv - Biochemistry 2020Quote: ... and GST or His6-MBP-tagged DI-DAX variants were transformed into BL21(DE3) Codon-Plus RIL Escherichia coli (Agilent, Santa Clara CA). A single colony or a scraping from a glycerol stock was used to inoculate a starter culture ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA was purified from total RNA using biotin-tagged poly dT oligonucleotides and streptavidin-coated magnetic beads and quality was assessed using an Agilent Technologies 2100 Bioanalyzer (Agilent, Santa Clara, CA).
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bioRxiv - Cancer Biology 2020Quote: ... Same amounts of early and late-labeled DNA were loaded on human DNA microarrays (SurePrint G3 Human CGH arrays, Agilent Technologies, G4449A). Hybridization was performed as previously described (39) ...
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bioRxiv - Biochemistry 2023Quote: ... Same amounts of early and late-labelled DNA were loaded on human DNA microarrays (SurePrint G3 Human CGH arrays, Agilent Technologies, G4449A). Hybridization was performed at 65°C as previously described43 ...
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bioRxiv - Genomics 2020Quote: ... HindIII-based Hi-C libraries from our previous study (Wutz et al., 2017) were captured using SureSelect target enrichment system (Agilent Technologies) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... the eight genes flanked with the same promoters and terminators were cloned into the 2μ-based high-copy plasmids pESC-URA-USER and pESC-HIS (#217451, Agilent Technologies), resulting in pESC-URA-USER harboring CYP79A2 ...
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bioRxiv - Biophysics 2020Quote: ... Human SHP-1 with an N-terminal 6x His tag was produced in Escherichia coli BL21-CodonPlus (DE3)-RIPL strain (Agilent Technologies) and purified on Ni2+-NTA agarose (Invitrogen ...
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bioRxiv - Evolutionary Biology 2021Quote: Evolved viral genomes of the passage 12 from each lineage and genotype were amplified by high-fidelity RT-PCR using the AccuScript Hi-Fi (Agilent Technologies) reverse transcriptase and Phusion DNA polymerase (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were subjected to C18 desalting using an Agilent 1260 Infinity LC system equipped with an MRP-C18 Hi-recovery column (Agilent, USA) before SpeedVac drying.
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bioRxiv - Genomics 2023Quote: The fragment size distribution and concentration of the final PacBio and Dovetail Hi-C libraries were assessed using the TapeStation (Agilent Technologies) and the Qubit Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and analysed by HPLC Shimadzu, Prominence-I (Milton Keynes, UK) with an ion exchange column Hi PlexH (300 × 7.7 mm) (Agilent, Cheadle, UK) and 0.6 mL min-1 flow rate at 55 ºC (oven temperature ...
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bioRxiv - Cell Biology 2020Quote: ... For the anti-human Fibronectin immunostaining (Dako, 1:1000) a single immunostaining was performed with a goat anti-rabbit secondary antibody and Alexa-568 phalloidin ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-human Chorionic Gonadotropin (hCG, A0231, Dako, 1:300), anti-ENDOU (HAP067448 ...
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bioRxiv - Cancer Biology 2021Quote: ... A Universal Human Reference (UHR) (Chem-Agilent, Catalog #740000) was sequenced with each batch of samples to allow for assessment and removal of technical artifacts (due to ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA samples were performed to Microarray analysis by Agilent SurePrint G3 human gene expression Microarray 8X60K (Agilent Technologies). After hybridization ...
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bioRxiv - Genetics 2022Quote: Preparation of the universal human reference RNA (Agilent, 74000) was done according to the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2020Quote: ... Library preparation (Agilent SureSelect Human All Exon V6 kit) and high throughout sequencing (Illumina NovaSeq 6000 ...
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bioRxiv - Cell Biology 2021Quote: ... and IgA (rabbit anti-human, Dako, A0262, 1:75). For amplified targets the appropriate HRP-conjugated secondary was then incubated at 1:250 in PBS for 45 minutes-1hr followed by TSA fluorophore treatment (Akoya) ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-human Chorionic Gonadotropin (CBG, A0231, Dako, 1:1000), anti-ENDOU (HAP067448 ...
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bioRxiv - Cancer Biology 2020Quote: ... tumor sections were stained for human Ki67 (Dako, #M7240) using the Target Retrieval Solution pH 9.0 (Dako ...
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bioRxiv - Neuroscience 2022Quote: ... wild type human TTC3 in pCMV-Tag2 vector (Stratagene), mouse AUTS2-myc-DDK in pCMV6 (OriGene ...
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bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-human CD31 (Agilent, clone DAKO JC70A), mouse monoclonal anti-HLA class II-DR/DP/DQ (Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-human CD31 (Agilent, clone DAKO JC70A), mouse monoclonal anti-HLA class II-DR/DP/DQ (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... and immunolabelled for human CD31 (Dako, JC70A, 1:50), VE-Cadherin (1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal CD31 anti-human clone JC70A (M0823, Agilent Dako ...
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bioRxiv - Microbiology 2022Quote: ... Sections were stained with mouse anti human CMV (Dako, Agilent technologies ...
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bioRxiv - Immunology 2023Quote: ... human albumin (diluted 1:1000; Dako, Carpinteria, CA, USA), complement C3 (diluted 1:1000 ...