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Citations for Agilent :
151 - 200 of 1811 citations for Hepatitis E Virus Capsid Protein ORF2 Mouse Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 96 deep well polypropylene plates (0.7 ml) with flat bottom and square wells (EK-2074, E&K Scientific; 201242-100, Agilent), or 24 well cell culture clear plates with flat bottom and circular wells (T1024 ...
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bioRxiv - Microbiology 2023Quote: Vero TMPRSS2 cells were plated in 96-well tissue culture treated E-Plate VIEW plates (#300-601-020, Agilent) to be subconfluent at time of assay ...
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bioRxiv - Cancer Biology 2023Quote: All cytotoxicity assays were performed using the xCELLigence RTCA MP analyzer and 96 well PET E-plates (Agilent Technologies). Assays were carried out in RPMI containing 10% fetal calf serum ...
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bioRxiv - Developmental Biology 2023Quote: ... Periodic acid-Schiff (PAS) and hematoxylin and eosin (H&E) staining were performed using standard procedures (Dako, Glostrup, Denmark) for the assessment of normal structures.
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bioRxiv - Neuroscience 2021Quote: ... was fused with a six histidine tag (6xHis) and an AviTag (pET-28a-AviTag-GluN1C0) and expressed in BL21-CodonPlus (DE3)-RIPL cells (Agilent). Expression of His-AviTag-GluN1(834-863 ...
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bioRxiv - Biochemistry 2019Quote: ... All additional tags were added via Gibson assembly and all mutations and truncations were made via site-directed mutagenesis (Agilent). For rapalog induced motility in cells ...
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bioRxiv - Biochemistry 2020Quote: ... Salmonella typhimurium MsbA (T561C in a C88A/C315A cysteine-less background) with an N-terminal poly-histidine-tag was expressed in BL21-CodonPlus (DE3)-RIPL (Agilent). Expression was induced with 1 mM IPTG for 4 hours at 30°C and membrane proteins were solubilized with 1% DDM and 0.04% sodium cholate in a buffer containing 100 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... and pCMVTag 4 expression control plasmid containing the firefly luciferase gene fused with the FLAG tag (luc-FLAG) were obtained from Stratagene. The plasmid 15cxCAT contained the interleukin-2 (IL-2 ...
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bioRxiv - Biochemistry 2022Quote: ... BioID2-tagged variants were generated by introducing a BamHI site between the 8xHis and MBP tags in the 8xHis-MBP-pFastBac1 modified with a CMV promoter vector by Quikchange Lightning site-directed mutagenesis (Agilent) and cloning BioID2 into the BamHI sites using In-Fusion EcoDry cloning according to manufacturer protocols.
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bioRxiv - Biophysics 2022Quote: ... The insertion of an amber codon (TAG) between E715 and V716 in mGluR2 (azi-ECL2) was performed using the QuikChange site-directed mutagenesis kit (Agilent). SNAP-mGluR2 constructs used for calcium imaging had C-terminal FLAG-tag removed by PCR-based deletion using phosphorylated primers ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting DNA fragments were cloned in frame with the FLAG tag using the oligonucleotides that are indicated in Supplementary file 2 into the pESC-URA vector (Agilent), in which the GAL10 and GAL1 promoters were replaced with the Cup1 promoter ...
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bioRxiv - Microbiology 2019Quote: ... a BglII restriction site within the linker-sequence separating the N-terminal mCherry-tag from hGBP1 was eliminated in pmCherry-hGBP1 by Quickchange Site Directed Mutagenesis (Agilent) using the oligomer pair pmCherry-hGBP1DBglII-F and -R (Table S9) ...
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bioRxiv - Cell Biology 2021Quote: ... All additional tags were added via Gibson assembly and all mutations and truncations were made via site-directed mutagenesis (Agilent).
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bioRxiv - Molecular Biology 2021Quote: ... 6XHis tag was added to the N-terminus of Upf1 in pGEM-3Zf (+) using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) with oligonucleotides 5-N-His-UPF1 and 5-N-HIS-UPF1-r to yield pGEM3Zf(+)-6XHis-UPF1 ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-NBR1 was generated by clone the NBR1 sequence from GFP-NBR1 into pCMV-Tag 2C vector (Agilent Technologies, #211172). pGEX6P was purchased from Millipore Sigma ...
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bioRxiv - Plant Biology 2023Quote: PHDsuper-FN3VRN5 from Zm for XL-MS analysis was inserted into pEC-LIC-His-3C containing a hexa-histidine tag and 3C protease cleavage site at the N terminus and was expressed in BL21 CodonPlus (DE3)-RIL cells (Agilent) in LB medium ...
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bioRxiv - Biophysics 2023Quote: Human BRAF kinase domain (residues 448-723 with a tobacco etch virus (TEV)-protease-cleavable N-terminal 6x-His tag in pProEX containing 16 mutations to improve solubility38 was expressed in chemically competent BL21 (DE3) RIL Escherichia coli (Agilent) for 18 h at 18°C ...
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bioRxiv - Biophysics 2023Quote: Human MEK1 kinase domain (residues 1-393 with a TEV protease cleavable N-terminal 6x-His tag in pET-Duet) containing an inactivating K97R mutation was expressed in chemically competent BL21 (DE3) RIL Escherichia coli (Agilent) for 18 h at 18°C ...
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bioRxiv - Biochemistry 2024Quote: ... and subsequently cloned into a pET-SUMO expression vector with an additional C-terminal hexahistidine tag cloned in using QuikChange (Agilent).
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bioRxiv - Immunology 2020Quote: ... protein block (Dako X0909) for 20 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: ... and Protein block (Dako) before staining with anti-pS6 (detected with fluorochrome conjugated secondary antibodies ...
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bioRxiv - Immunology 2022Quote: ... Influenza A virus inactivation was performed by delivering 2400 μJ/cm2 of UV light (254 nm) using a Strata linker 1800 (Stratagene, La Jolla, CA.). Virus inactivation was verified by plaque assay ...
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bioRxiv - Molecular Biology 2024Quote: M280 anti-mouse magnetic beads were coupled to control mouse IgG2a (Dako) or mouse monoclonal anti-Ku antibodies (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: Microscopic observation of Arabidopsis plantlets attacked with pathogens was performed using an inverted spinning disk confocal microscope with a high-resolution camera (Yokogawa CSU-X1 on Nikon Ti-E platform, laser box Agilent MLC400 ...
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bioRxiv - Cancer Biology 2021Quote: ... Animals treated with CAR T cells had tumors excised and were also stained with H&E and human CD3 (A0452, Dako), in addition to GPC2 ...
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded tissue sections were dewaxed and stained according to the H/E standard protocol using a CoverStainer (Dako, Agilent).
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded tissue sections were dewaxed and stained according to the H/E standard protocol using a CoverStainer (Dako, Agilent).
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bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
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bioRxiv - Immunology 2022Quote: ... 50 µL of Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 2% FBS was added to each well of a 96-well E-plate (Agilent) to establish the background reading ...
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bioRxiv - Immunology 2022Quote: ... 50ul of RPMI-1640 was first added to each well of the 96-well E-plates (Agilent, Santa Clara, CA) and was measured as background impedance ...
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bioRxiv - Genomics 2023Quote: ... the slide was baked at 60 °C for 60 minutes and then processed through the Dako Progressive H&E protocol (Agilent) and using Dako Harris Hematoxylin ...
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bioRxiv - Immunology 2022Quote: ... anti-mouse-HRP (Dako) secondary Abs or StrepTactin-HRP (IBA-LifeSciences ...
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bioRxiv - Microbiology 2022Quote: ... mouse anti-hCK18 (Agilent, Santa Clara ...
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bioRxiv - Cell Biology 2021Quote: ... -goat anti-mouse (Dako).
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bioRxiv - Molecular Biology 2022Quote: ... Rabbit/Mouse (Dako, Denmark).
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bioRxiv - Developmental Biology 2023Quote: ... p63 (mouse, DAKO, M7317). Sections were extensively washed in 1X PBS and incubated with secondary antibodies (Alexa Fluor 488 anti-rabbit A11008 ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-mouse (Agilent #P0260) or anti-rabbit (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse envision (K4001, Agilent) diluted 1:3 with PBS was used for detection of IgG ...
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bioRxiv - Cell Biology 2020Quote: ... and Pnut with a C-terminal Strep tag II (WSHPQFEK, 1058 Da) were purified from Escherichia coli BL21(DE3) cells (Agilent Technologies) using a two-tag affinity purification scheme to aid the isolation of full-length complexes as explained in ref ...
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bioRxiv - Biophysics 2020Quote: ... Human SHP-1 with an N-terminal 6x His tag was produced in Escherichia coli BL21-CodonPlus (DE3)-RIPL strain (Agilent Technologies) and purified on Ni2+-NTA agarose (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... OPG2-E was generated by cloning the cDNA encoding the E-protein into the same pcDNA5-OPG2 vector using the KpnI and BamHI sites and deleting the stop codon after the OPG2 tag by site-directed mutagenesis (Stratagene QuikChange, Agilent Technologies). The N-terminal OPG2-tag of OPG2-ORF6-OPG2 was inserted by site-directed mutagenesis of ORF6-OPG2 using the relevant forward and reverse primers (Integrated DNA Technologies) ...
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bioRxiv - Neuroscience 2019Quote: ... pCMV-Tag-3B-2XMyc-LRRK2-D1994A was generated by mutagenesis of pCMV-Tag-3B-2XMyc-LRRK2-WT using a QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... hFN10 containing a TAG stop codon at position 1493 was generated by PCR-based mutagenesis with the Quick-change kit (Agilent Technologies), cloned into bacterial expression plasmid pET11a and verified by DNA sequencing ...
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bioRxiv - Neuroscience 2019Quote: ... containing full-length rat Syt1 and a preprotachykinin signal sequence cloned upstream of the Myc tag as described in ref.17 The F349A mutation was introduced using site directed mutagenesis (QuickChange, Agilent Technologies). Myc-tagged Syt1WT and Syt1F349 sequences were then subcloned into the lentiviral vector L309 (a gift from T ...
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bioRxiv - Molecular Biology 2023Quote: ... The sequence was mutagenized to obtain the N-ter tag canonical variant 2 through the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, 200521) using the following DNA primers ...
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bioRxiv - Microbiology 2023Quote: ... AIP56D274S/D276-278S and AIP56D274N/D276-278N with or without V5-tag were generated with the QuickChange Site-Directed Mutagenesis Kit (Stratagene, 200518) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... A 10-minute protein block step with Serum-Free Protein Block (X0909, Agilent DAKO) to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK ...
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bioRxiv - Neuroscience 2020Quote: ... A 10-minute protein block step with Serum-Free Protein Block (X0909, Agilent DAKO) to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK ...
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bioRxiv - Immunology 2021Quote: ... we changed three amino acids within the Fc region by using the QuikChange II site directed mutagenesis kits (Agilent, #200523), introducing M257Y ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with 1 μg/mL recombinant anti-PSD95 antibody (Rabbit Fc fusion, NanoTag Biotechnologies #N3783) diluted in Dako REAL Antibody Diluent (S2022, Agilent) for 1 h at RT ...