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Citations for Agilent :
151 - 200 of 1324 citations for 6 Amino 2 chloro 9 methylpurine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Accuscript reverse transcriptase (6×10−5) (Agilent, product literature), and Phusion DNA polymerase after 20 cycles of amplification (1.2×10−5 ...
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bioRxiv - Microbiology 2019Quote: ... GFAP (Dako #M076101-2 or #Z033429-2), OLIG2 (R&D Systems #AF2418 ...
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bioRxiv - Bioengineering 2021Quote: ... Mutagenesis of individual amino acid residues in KRaION1 was done using the QuikChange Site-Directed Mutagenesis Kit (Agilent). Forward and reverse primers used to generate each mutant are provided in Supplementary Table 6 ...
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bioRxiv - Cell Biology 2020Quote: ... ShhHA and C25SShhHA (HA inserted between amino acids 197 and 198) were generated by site-directed mutagenesis (Stratagene). Unlipidated C25SShhN cDNA and non-palmitoylated C25AShh cDNA (amino acids 1-438 ...
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bioRxiv - Molecular Biology 2023Quote: ... Amino acid substitutions in the C-terminal (126-250) truncation were created via QuikChange site-directed mutagenesis (Agilent). pDEST-mCherry-LacR-RIF1 (1-567 ...
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bioRxiv - Plant Biology 2023Quote: ... The chromatographic separations of free amino acids were carried out using a HILIC-Z column (2.1×150 mm, particle size 2.7 µm, Agilent) employing a linear binary gradient of (A ...
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bioRxiv - Neuroscience 2023Quote: ... Single amino acid substitutions of GLIC were engineered using the QuikChange II Site-Directed Mutagenesis kit (Agilent Technologies) and oligonucleotide primers (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... All samples had an RNA Integrity (RIN) score of >9 as determined by the Agilent 2100 bioanalyzer or Agilent 2200 Tapestation system (Agilent). For RNAseq of cell lines (Figure 5C) ...
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bioRxiv - Molecular Biology 2022Quote: ... and DDX60L cDNAs were amplified from either a HeLa-JVM or HEK293T total cDNA library and inserted into the NotI and XhoI restriction sites in the pCMV-3Tag-9 vector (Agilent Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... A frequency of 59.673 MHz and an amplitude of 8 dBm was generated by a signal generator (EXG Analog Signal Generator, NS171B, 9 kHz – 3 GHz, Agilent) and fed into the transmitter and reception boards of the scanner via a respective custom made power splitter.5
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bioRxiv - Neuroscience 2021Quote: ... 3 μm paraffin-embedded tissue sections were either dewaxed and subjected to antigen retrieval treatment with Tris-EDTA buffer pH 9 for 20 min at 97°C using a PT Link (Dako – Agilent) or dewaxed as part of the antigen retrieval process using the Low pH EnVision ™ FLEX Target Retrieval Solutions (K8005 ...
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bioRxiv - Cancer Biology 2019Quote: ... MSH2 and PMS2 and at pH 9 for MSH6) using a detection system suitable for the Dako Autostainer Link 48 (EnVision FLEX, Dako).
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bioRxiv - Cell Biology 2020Quote: ... Prior to immunohistochemistry antigen retrieval was performed using Tris-EDTA buffer pH 9 for 20 min at 97°C using a PT Link (Dako – Agilent). Quenching of endogenous peroxidase was performed by a 10 min incubation with Peroxidase-Blocking Solution (Dako REAL S2023) ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids expressing gfpA206K fusions to mgrB mutants (pSY3-9, pSY72-75, pJC1-7, pTG1-15) were created by either QuikChange site-directed mutagenesis (Stratagene) or Inverse PCR [58] using pAL38 as template ...
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bioRxiv - Genomics 2022Quote: ... Hi-C material was then amplified from the beads with 7-9 cycles of PCR using Herculase II Fusion DNA polymerase (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Tissues next underwent antigen retrieval was carried out by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating them at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Heat-mediated epitope retrieval was performed by incubating slides in a beaker containing Tris EDTA solution (pH 9; Dako, #S2367) in a pressure cooker for 20 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... HEK-293T cells were co-transfected with the following plasmids: pAAV/L7-6-Slc9A6-GFP or pAAV/L7-6-GFP (control) with pHelper (Stratagene, La Jolla, CA, USA), and pUCmini-iCAP-PHP.eB followed by viral particles purification ...
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bioRxiv - Neuroscience 2019Quote: ... of the canonical 307 amino acid human NMNAT2 variant (NCBI ProteinID: NP_055854) cloned in expression vector pCMV Tag-2B (Stratagene). A short linker (17 amino acids ...
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bioRxiv - Biochemistry 2021Quote: ... Selected residues were mutated to alanine or oppositely-charged amino acids by PfuUltra II Hotstart PCR Master Mix (Agilent). All mutated sequences were confirmed by Sanger sequencing ...
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bioRxiv - Microbiology 2020Quote: ... The codon at amino acid position 160 in HA (H3 numbering, Threonine) was modified via site-directed mutagenesis (Agilent) from the wild type (ACA ...
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bioRxiv - Microbiology 2022Quote: ... Amino acid substitutions and deletions were introduced into pET28b vectors with the QuickChange II Site-Directed mutagenesis kit (Stratagene), according to the manufacturer’s directions ...
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bioRxiv - Biophysics 2023Quote: ... The Pro283Ser amino substitution was introduced to this vector via the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) as previously described (13) ...
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bioRxiv - Plant Biology 2024Quote: ... Separation of amino acid was done with a Zorbax Eclipse XDB-C18 column (50 × 4.6 mm, 1.8 μm, Agilent Technologies). The mobile phase comprised of solvent A (water ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Amino acid deletions for ΔKPQ were generated by use of the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent, USA). PCR products were cloned into XL-10 Gold Ultracompetent cells ...
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bioRxiv - Developmental Biology 2020Quote: ... Guinea Pig anti-Insulin pre-diluted 1:6 (Dako 1R002), Chicken anti-GFP 1:1000 (Abcam ab13970) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... low pH∼6 (Dako, K8005; Agilent, Santa Clara, CA, USA), with preheating to 80°C and increased temperature to 95°C for 20min after slides were added ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... low pH∼6 (Dako, K8005; Agilent, Santa Clara, CA, USA), with preheating to 80°C and increased temperature to 95°C for 20min after slides were added ...
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bioRxiv - Cancer Biology 2021Quote: ... cytokeratin 5/6 (mouse monoclonal, clone 6D5/16 B4, Dako), epidermal growth factor receptor (EGFR ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were treated with AEVs from AD and normal participants for 1 hour and then subjected to fluorescent immunolabelling for MAC using a mouse anti-human C5b-9 antibody (1 μg/mL; RRID: AB_2067162, cat. no. M0777, Dako, Carpinteria, CA) targeting an epitope in the poly-C9 component of MAC in association with C5b ...
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bioRxiv - Plant Biology 2020Quote: ... RT-PCR was performed with 1μL of diluted cDNA (1:9) in a Brillant III ultra fast SYBRgreen QPCR master mix (Agilent Technologies, USA) using a StepOnePlus Real-Time PCR System (Applied biosystems ...
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bioRxiv - Microbiology 2021Quote: ... and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 12 μM medronic acid (InfinityLab Deactivator additive, Agilent Technologies). The flow rate was kept at 250 μl/ml consisting of a 2 minutes hold at 10% B ...
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bioRxiv - Microbiology 2021Quote: ... and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive, Agilent Technologies). The flow rate kept at 250 μl/ml consisting of a 2 minutes hold at 10% B ...
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bioRxiv - Neuroscience 2021Quote: ... 3 μm paraffin-embedded tissue sections were either dewaxed and subjected to antigen retrieval treatment with Tris-EDTA buffer pH 9 for 20 min at 97°C using a PT Link (Dako – Agilent) or dewaxed as part of the antigen retrieval process using the Low pH EnVision ™ FLEX Target Retrieval Solutions (K8005 ...
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bioRxiv - Cell Biology 2020Quote: ... Prior to immunohistochemistry antigen retrieval was performed using Tris-EDTA buffer pH 9 for 20 min at 97°C using a PT Link (Dako – Agilent). Quenching of endogenous peroxidase was performed by a 10 min incubation with Peroxidase-Blocking Solution (Dako REAL S2023) ...
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bioRxiv - Immunology 2019Quote: ... Antigen retrieval was performed either with Proteinase K (for F4/80) or boiling the sections in a high pH antigen retrieval buffer (Dako Target retrieval Solution, pH 9, Dako, Denmark) (for Podocalyxin ...
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bioRxiv - Microbiology 2022Quote: ... and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive, Agilent Technologies). The flow rate was kept at 250 μL mL−1 consisting of a 2 min hold at 10% B ...
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bioRxiv - Neuroscience 2022Quote: ... of RF energy with varying input power to the antenna (9-68 W, measured by a power meter (U2001A Power Sensor connected to N9912A Field Fox Spectrum analyzer, Agilent/Keysight) and a bidirectional coupler (778D ...
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bioRxiv - Neuroscience 2024Quote: ... 6.5-9) and concentration (5-50 ng/µl; 260/280 values >1.7) were evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, CA) and Nanodrop (Thermo-fisher ...
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bioRxiv - Immunology 2024Quote: ... Epitope retrieval was then performed at 95 °C for 10 min at pH 9 with Dako Target Retrieval Solution (Agilent, USA), in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2023Quote: ... Tissues next underwent antigen retrieval was carried out by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating them at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Heat-induced antigen retrieval (HIER) was performed in a coverslip jar containing 1x Dako pH 9 Antigen Retrieval Buffer (Agilent, S2375) while using a PT module filled with 1x PBS ...
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bioRxiv - Biochemistry 2024Quote: ... 120-73-0) and 2.5 mmol/L hexakis(1H, 1H, 3H-tetrafluoropropoxy)phosphazine (HP-0921, CAS No. 58943-98-9) (Agilent, Cheadle, UK).
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bioRxiv - Microbiology 2020Quote: ... The 75th amino acid in the PB1-F2 protein was changed to a histidine (H) via site directed mutagenesis (QuikChange Lightning, Agilent) to produce the PB1-F2-75H plasmid ...
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bioRxiv - Plant Biology 2020Quote: ... and fluorenylmethoxycarbonyl (FMOC) was based on the application note “Automated amino acids analysis using an Agilent Poroshell HPH-C18 Column” by Agilent. The samples were injected onto a 100 mm x 3 mm InfinityLab Poroshell HPH-C18 column (2.7 μm ...
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bioRxiv - Immunology 2019Quote: Purified recombinant His-tagged C-terminal MSP1 protein (amino acids 4960 to 5301) (Ndungu et al., 2009) was biotinylated and tetramerized with streptavidin-PE (Prozyme), as previously described (Krishnamurty et al. ...
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bioRxiv - Genetics 2019Quote: ... into the complete open reading frame of the canonical 307 amino acid human NMNAT2 isoform cloned into expression vector pCMV-Tag2 (Stratagene). The expressed NMNAT2 proteins have a Flag tag and short linker sequence (17 amino acids ...