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Citations for Agilent :
1851 - 1900 of 6766 citations for Mouse Protein DEK DEK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... using the QuikChange Site-Directed Mutagenesis kit (Agilent Technologies) and mutagenic primers listed in Supplementary Table 3.
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bioRxiv - Cancer Biology 2024Quote: ... along with the mitochondrial stress kit (Agilent, California, USA). This approach aimed to measure the oxygen consumption rate (OCR ...
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bioRxiv - Cancer Biology 2024Quote: ... using a Seahorse XF Glycolytic Rate Assay Kit (Agilent) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... QuikChange II Site-Directed Mutagenesis Kit (Agilent, 200523-5) was used for mutagensis of GFP-VPS35L constructs following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... according to manufacturer’s protocols (Mito Stress Test Kit, Agilent). For real-time ATP production ...
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bioRxiv - Genomics 2024Quote: ... and the High Sensitivity DNA Assay kit (Agilent Technologies), respectively ...
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bioRxiv - Developmental Biology 2024Quote: ... A QuickChange Site-Directed Mutagenesis Kit (Agilent, Cat # 200524) was employed to generate the designated deletions and mutations of the putative binding sites within the 3’reg1-nr2f1a:GFP reporter vector ...
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bioRxiv - Genomics 2024Quote: We used the QuikChange Site-Directed Mutagenesis Kit (Agilent) as directed to generate the M03A1.3 3’UTR mutants used in this study ...
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bioRxiv - Immunology 2024Quote: ... and High Sensitivity DNA Kit (Agilent Technologies, #5067-4626). DNA libraries were then prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Immunology 2024Quote: ... and High Sensitivity DNA Kit (Agilent Technologies, #5067-4626). Libraries were sequenced by Genewiz on an Illumina HiSeq 4000 by 2×75 bp paired end sequencing.
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bioRxiv - Cell Biology 2023Quote: ... Seahorse XF Glycolysis Rate Assay Kit (#103344-100, Agilent) was used ...
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bioRxiv - Molecular Biology 2024Quote: ... and Mito-stress test kit (Agilent, Cat# 103015-100) along with Seahorse XFe96/XF Pro FluxPak (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... The 75th amino acid in the PB1-F2 protein was changed to a histidine (H) via site directed mutagenesis (QuikChange Lightning, Agilent) to produce the PB1-F2-75H plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... MBP-Tlg21-327 and the co-expressed Vps45–Tlg2 proteins were expressed and purified in a similar manner with the following modifications: protein was overexpressed in BL21-Codon Plus (Agilent); after IPTG addition the cells were grown at 16°C ...
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bioRxiv - Cell Biology 2020Quote: Samples from all biological replicates were first sent to the Stanford University Protein and Nucleic Acid Facility (Stanford, CA) for quantification and quality analysis using a 2100 Bioanalyzer (Agilent). Samples were then sent to Novogene Corporation Inc ...
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bioRxiv - Biophysics 2021Quote: ... The plasmid encoding the protein fused with a His-tag at the N-terminus was transformed in BL21 DE3 pLysS strains (Agilent). Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were washed for 5 minutes using 1X Wash Buffer and then blocked by adding 8 drops of Protein Block Serum-Free (Agilent). Primary antibodies were diluted in diluent (Dako ...
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bioRxiv - Molecular Biology 2021Quote: Affinity purification and digestion of biotinylated proteins were carried out in an automated fashion in a Bravo AssayMap platform (Agilent) using AssayMap streptavidin cartridges (Agilent) ...
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bioRxiv - Immunology 2019Quote: Purified recombinant His-tagged C-terminal MSP1 protein (amino acids 4960 to 5301) (Ndungu et al., 2009) was biotinylated and tetramerized with streptavidin-PE (Prozyme), as previously described (Krishnamurty et al. ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The multiple charged peaks of the antibody were deconvoluted using the Agilent MassHunter Bioconfirm software (deconvolution for protein, Agilent technology) with the deconvolution range from 20 kDa to 160 kDa ...
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bioRxiv - Systems Biology 2021Quote: ... The relative secreted expression of ARTN was determined by western blotting of culture supernatants using the rabbit antibody HPRK3140989 from the Human Protein Atlas and an HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Systems Biology 2021Quote: ... rabbit anti-THBS4 (antibody HPRK2400008 kindly provided from the Human Protein Atlas) as primary antibody and a HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... Samples were washed with PBS twice and were either blocked for 10 min at room temperature (Dako protein block #X0909), or with goat block (GB ...
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bioRxiv - Physiology 2021Quote: ... Samples were washed with PBS twice and were either blocked for 10 min at room temperature (Dako protein block #X0909), or with goat block (GB ...
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bioRxiv - Microbiology 2020Quote: Proteins were detected using the following primary antibodies: anti-enterovirus VP1 clone 5-D8/1 antibody purchased from Dako (Denmark); Mouse anti-Ub ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Seahorse data were normalized to total amount of protein (μg) and mitochondrial respiration parameters were analyzed using Wave Desktop 2.6 software (Agilent Technologies).
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bioRxiv - Molecular Biology 2020Quote: ... The free HSF2BP protein as well as the complex between ARM and F15X were analyzed using a Bio SEC-3 column (Agilent) equilibrated in 25 mM Tris-HCl buffer ...
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bioRxiv - Cell Biology 2020Quote: The cells were fixed with 4% paraformaldehyde at 4°C for 5 min and permeabilized with 0.1% Triton X-100 at room temperature for 20 min in the presence of a protein-blocking solution consisting of PBS supplemented with 5% normal goat serum (Agilent Technologies ...
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bioRxiv - Plant Biology 2022Quote: His6-MBP-PFA-DSP protein fusions or free His8-MBP were expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells (Stratagene). Overnight bacterial cultures were inoculated 1:1000 into fresh 2YT medium (1.6 % tryptone ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed with TBST (Tris Borate Saline Tween-20) and then blocked with Protein Block Serum-Free (Ref: No X0909, Dako) for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... A plasmid coding for the fusion protein with a K1432A substitution in the RemA CTD was made by the QuikChange mutagenesis (Agilent) using primers PG007 and PG008.
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bioRxiv - Biochemistry 2020Quote: ... and the fluorescence signals corresponding to temperature-dependent protein unfolding were measured using a Real-Time PCR Mx3005p machine (Stratagene). The melting temperature calculation was performed as described previously46.
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bioRxiv - Biophysics 2022Quote: 30 µl EPYC1 and 30 µl Rubisco proteins were mixed in the cuvette for measurement in the Cary 60 UV-Vis spectrophotometer (Agilent). After 10 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... and the fluorescence signals corresponding to temperature-dependent protein unfolding were measured using a Real-Time PCR Mx3005p machine (Stratagene). Melting temperature shifts were calculated using the previously described method61.
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bioRxiv - Neuroscience 2021Quote: ... to test the presence of electrical coupling between THINs and NGF interneurons we used double transgenic TH–Cre crossed with (BAC) transgenic mice that express the humanized Renilla green fluorescent protein (hrGFP) (Stratagene) under the control of the mouse NPY promoter (NPY-GFP ...
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bioRxiv - Pathology 2020Quote: ... Sections were allowed to thaw at RT and thereafter blocked for > 60 min at RT with blocking-buffer (serum-free protein blocking solution, DAKO), supplemented with 0.2% Triton X-100 (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2020Quote: 100 μL of purified recombinant protein (at approximately 2 mg/mL) were loaded onto a sizeexclusion chromatography column (PL1580-3301, Agilent) in the phosphate buffer (20 mM Tris ...
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bioRxiv - Biophysics 2019Quote: ... Major interfering proteins were removed from the serum by the Multiple Affinity Removal Spin Cartridge HSA/IgG (Agilent Technologies, USA) as described into manual ...
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bioRxiv - Bioengineering 2019Quote: ... The primary antibodies sources and dilutions were as follows: rabbit poly-clonal anti-glia fibrillary acidic protein (GFAP; 1:500; DAKO), mouse monoclonal anti-Neuronal Class III B-Tubulin (TuJ1 ...
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bioRxiv - Biophysics 2020Quote: ... bound to precursor protein was analysed by SDS-PAGE and liquid chromatography coupled to mass-spectrometry (LC ESI-TOF MS, 6210, Agilent Technologies ...
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bioRxiv - Biophysics 2020Quote: His-tagged CrSAS-6[NL] spanning amino acids 1-503 of the protein (see Supplementary Fig. 1a)1 was expressed in the Escherichia coli strain BL21(DE3) (Stratagene). Bacteria were grown at 37 °C in lysogeny broth (LB ...
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bioRxiv - Immunology 2021Quote: ... and by SEC-HPLC with an xbridge protein BEH SEC (Waters, ref. 186009160) connected to a HP1100 system (Agilent Technologies).
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bioRxiv - Molecular Biology 2022Quote: ... whole protein lysates for each well were quantified by BCA assay and total protein amounts were used for normalization of Seahorse data using Wave Software (Agilent). Basal OCR ...
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bioRxiv - Biochemistry 2022Quote: ... constructs containing DNA encoding 8X-His-tagged CHI-domain-containing proteins were transformed into BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent) for recombinant protein expression ...
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bioRxiv - Bioengineering 2022Quote: ... and incubated overnight at 4 °C with primary antibodies for the glial fibrillary acidic protein (GFAP; 1:1000, Z0334, Dako), ionised calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Molecular Biology 2023Quote: The above 12 OR genes linked to a wild-type internal ribosome entry site from the encephalomyocarditis virus (EMCV IRES) and the mCherry fluorescent protein gene (Shaner et al., 2004) were inserted into a modified pBlueScript KS (Agilent/Stratagene) called plasmid “L” (Zboray et al. ...
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bioRxiv - Biochemistry 2023Quote: ... CLEANEX and DOSY data were collected on 100 μM 15N-labelled protein at a proton Larmour frequency of 600 (Agilent) or 800 (Bruker ...
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bioRxiv - Microbiology 2023Quote: Yeast proteins under both conditions were extracted and later subjected to fractionation through 3100 OFFGEL (Agilent Technologies, Palo Alto, CA) followed by an identification by LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Vectors encoding the recombinant proteins were constructed as described in the previous section and introduced into the BL21-CondonPlus (DE3)-RIL competent cells (Agilent Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were incubated overnight with the following primary antibodies: rabbit polyclonal anti-glial fibrillary acidic protein (GFAP) (1:6,000, DAKO, UK), rabbit polyclonal anti-allograft inflammatory factor 1 (IBA1 ...