Labshake search
Citations for Agilent :
1801 - 1850 of 4338 citations for N 5 Trimethoxysilyl 2 Aza 1 Oxopentyl Caprolactam since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Cells were then washed in PBS 5 x 5 mins, then mounted with a cleaned glass coverslip (#1.5, 24 mm x 60 mm) in mounting media (Dako; Pathology Products, S3023).
-
bioRxiv - Molecular Biology 2023Quote: ... column protected with an ZORBAX RRHD Eclipse Plus C18 5 x 2.1 mm ID (1.8 µm) guard column (Agilent Technologies, Foster City, CA). The mobile phase consisted of water and methanol (both added 0.1% formic acid ...
-
bioRxiv - Neuroscience 2023Quote: ... Pools were diluted to 5 ng/µL and quality and size are assessed using Agilent DNA High Sensitivity chip (Agilent Technologies, Inc.) on the Bioanalyzer ...
-
bioRxiv - Molecular Biology 2023Quote: A fluorescent image of rehydrated brain tissue with a thickness of 275 μm stained with Amy tracker and placed on glass microscopic slides was acquired using the manual mode in a Cytation 5 multimode reader (Agilent Technologies, USA). Montage images were collected at 4x and 20x magnification ...
-
bioRxiv - Genetics 2023Quote: ... Kallisto quant settings were adjusted to -b 5 -l 160 -s 20 - -single - -threads 4 based on fragment lengths determined by the Agilent 4200 TapeStation (Agilent Technologies, Inc.)
-
bioRxiv - Molecular Biology 2023Quote: ... The dried labelled peptide plexed sample was then resuspended in 400uL of 1 M TEAB and fractionated using an Agilent 1100 HPLC system and high pH reversed phase chromatography on a Zorbax Eclipse XDB-C18 column (4.6 x 150mm, 5-micron) from Agilent (Santa Clara, CA). Sixty fractions were collected at 0.5 mL/min over 105 min using a gradient of 0-5% solvent B in 10 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... Blocking with endogenous peroxidase was performed with Protein Block (Novocastra Laboratories) for 5 min and subsequent steps were performed in DAKO autostainer link 48 (Agilent Technologies, Inc.). Slides were counterstained in hematoxylin-eosin ...
-
bioRxiv - Immunology 2023Quote: ... Bacteria were fixed with 0.5% formaldehyde at RT for 30 min and then wells were washed twice with a washer dispenser (BioTek EL406, Agilent Technologies, US) with PBS ...
-
bioRxiv - Bioengineering 2023Quote: ... were obtained by high pressure liquid chromatography on an Agilent Bio SEC-5 500 Å (for KWOCA assemblies) or 1000 Å column (for I32-10) (Agilent) at a flow rate of 0.35 mL/min by injection of 10 μL of purified eluate using a mobile phase of Tris-buffered saline (50 mM Tris pH 8 ...
-
bioRxiv - Neuroscience 2024Quote: ... The gas chromatograph was equipped with a 5 m guard column + 30 m (I.D. 250 μm, film 0.25 μm) DB-35MS capillary column (Agilent J&W GC Column). Helium was used as the carrier gas with a constant flow rate of 1.2 mL/min.
-
bioRxiv - Plant Biology 2024Quote: ... CMV genomic RNAs (strain Fny) were synthesized with 5’ monomethylated cap analog by T7 RNA Polymerase (Agilent Technologies, Santa Clara, CA, #600123) from PCR product templates (see Supplementary Table S1 for primer sequences) ...
-
bioRxiv - Bioengineering 2024Quote: ... and reverse primer (5’-AGGTCATGTACTGGGCATAAT-3’) binding to the GFP sequence with 5 µL of Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies, USA) and 0.15 µL of 2 µM reference dye.
-
bioRxiv - Neuroscience 2024Quote: ... Pools were diluted to 5 ng/μL and quality and size are assessed using Agilent DNA High Sensitivity chip (Agilent Technologies, Inc.) on the Bioanalyzer ...
-
bioRxiv - Molecular Biology 2024Quote: Serial sections of human and mouse FFPE BM biopsies were prepared at 3-5 μm thickness on coated microscope slides (Dako FLEX, Agilent) and processed for immunohistochemistry/immunofluorescent-based (IHC-IF) ...
-
bioRxiv - Biochemistry 2024Quote: ... containing the assembled ribosomes from 25 µL mixtures and 5 nM DNA template encoding sfGFP were incubated at 37 °C in Mx3005P (Agilent Technologies, USA), and sfGFP fluorescence was monitored during incubation.
-
bioRxiv - Biochemistry 2024Quote: ... containing the assembled ribosomes from 25 µL mixtures and 5 nM DNA template encoding sfGFP were incubated at 37 °C in Mx3005P (Agilent Technologies, USA), and sfGFP fluorescence was monitored during incubation.
-
bioRxiv - Cell Biology 2020Quote: ... followed by 1-hour incubation with peroxidase-labeled goat anti-mouse antibodies (1:1000; DAKO) and a 7 min incubation with the True Blue™ (KPL ...
-
bioRxiv - Microbiology 2020Quote: ... or chicken anti-GFAP (Abcam, 1:500, or mouse anti-CD68 (Agilent/Dako, 1:200) for 2 hours at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... or chicken anti-GFAP (Abcam, 1:500, or mouse anti-CD68 (Agilent/Dako, 1:200) for 2 hours at room temperature ...
-
bioRxiv - Developmental Biology 2024Quote: ... insulin (1:100; custom made by Cambridge Research Biochemicals) and somatostatin 1:300 (DAKO-A0566). Liver and insulin area was calculated on maximum projections using the Fiji threshold function.
-
bioRxiv - Physiology 2023Quote: ... freshly prepared) was flowed at 1 ml/min through a 1:100 flow splitter (Agilent Technologies 1260 Infinity II ...
-
bioRxiv - Cell Biology 2023Quote: ... freshly prepared) was flowed at 1 ml/min through a 1:100 flow splitter (Agilent Technologies 1260 Infinity II ...
-
bioRxiv - Immunology 2023Quote: ... EPCAM (CST, 1:5000), Claudin1 (SantaCruz, 1:200) and the bound secondary antibody – peridoxidase (DAKO) was detected with Liquid DAB + substrate chromogen system (DAKO ...
-
bioRxiv - Bioengineering 2024Quote: ... and resuspended in 1:1 acetonitrile:water before analysis using untargeted liquid chromatography mass spectrometry (Agilent 1290 LC coupled with Agilent 6538 Quadrupole-Time of Flight (Q-TOF ...
-
bioRxiv - Microbiology 2020Quote: FITC-conjugated rabbit F(ab’)2 anti-human C1q was obtained by digestion of FITC-conjugated rabbit anti-human C1q (Dako) using 1 U/ µg of recombinant His-tagged IdeS protease ...
-
bioRxiv - Cancer Biology 2021Quote: ... 2×105 cells/well were plated in five technical replicates on collagen-coated XF96 polystyrene cell culture microplates (Seahorse, Agilent) in GM ...
-
bioRxiv - Cell Biology 2020Quote: A library encoding ARHGAP36 isoform 2 mutants was created via error-prone PCR (epPCR) using the GeneMorph II Random Mutagenesis Kit (Agilent). To determine the optimal epPCR conditions for library generation ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2 µl of each purified final library was run on an Agilent TapeStation HS D1000 ScreenTape (Agilent Technologies, 5067-5584). The libraries were quantified using the Quant-iT 1X dsDNA HS kit (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... the cells were resuspended in 500 µl PBSF buffer (PBS+2% FBS) and analyzed using ACEA NovoCyte TM (Agilent Biosciences); non-transfected 293T cells served as a negative control.
-
bioRxiv - Cell Biology 2022Quote: ... Before separation peptides were first trapped (Dr Maisch Reprosil C18, 3 μm, 2 cm x 100 μm) and then separated on an analytical column (Agilent Poroshell EC-C18 ...
-
bioRxiv - Microbiology 2021Quote: ... D614G and other SARS-CoV-2 Spike single mutations were generated using the QuickChange II XL site-directed mutagenesis protocol (Stratagene) and the pCG1-SARS-CoV-2-S plasmid kindly provided by Stefan Pöhlmann ...
-
bioRxiv - Systems Biology 2021Quote: ... The relative secreted expression of ARTN was determined by western blotting of culture supernatants using the rabbit antibody HPRK3140989 from the Human Protein Atlas and an HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
-
bioRxiv - Systems Biology 2021Quote: ... rabbit anti-THBS4 (antibody HPRK2400008 kindly provided from the Human Protein Atlas) as primary antibody and a HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with TMB substrate (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... Mutant SARS-CoV-2 expression plasmids were generated by site-directed mutagenesis using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent). Unless otherwise stated all SARS-CoV-2 spike expression plasmids were based on the Wuhan-hu-1 reference sequence 41 ...
-
bioRxiv - Systems Biology 2021Quote: ... The staining was performed according to the manufacturer’s procedure with EnVision G|2 Doublestain System Rabbit/Mouse (DAB+/Permanent Red) kit (Dako/Agilent K5361) on the Dako Autostainer ...
-
bioRxiv - Cell Biology 2021Quote: RNAi-resistant EGFP-ORP5A and EGFP-ORP5B were generated by introducing 4 silent point mutations in the region targeted by the 2 siRNA oligos (#10 and #11) by site-directed mutagenesis (Quickchange II-XL, Stratagene) and the following primers:
-
bioRxiv - Biochemistry 2020Quote: ... Radioactivity was detected using an in-line Lablogic Radiodetector and the analysis was calibrated using a co-injected 2-aminobenzamide-labeled dextran ladder (ProZyme/Agilent) detected on a Dionex fluorescence detector.
-
bioRxiv - Plant Biology 2021Quote: ... The SEGS-2 ATG mutant (pNSB2110) was created in S2-1.5b (pNSB1869) using the QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies) and the primers ...
-
bioRxiv - Plant Biology 2021Quote: ... pGEX6P1:SYT1C2B (residues G374-end) and pGEX6P1:SYT1-SMPC2A (residues N34-E397) were transformed into Rosetta 2 (DE3) pLysS cells (Stratagene) for protein expression using standard protocols ...
-
bioRxiv - Microbiology 2021Quote: ... 2□μl of each sample was used to assess RNA quality using Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA), and the RNA integration numbers (RIN ...
-
bioRxiv - Microbiology 2021Quote: Plasmids encoding the single-mutation and the combination of mutations found in SARS-CoV-2 variants were generated by Quikchange II XL site-directed mutagenesis kit (Agilent). Recombinant Indiana vesicular stomatitis virus (VSV ...
-
bioRxiv - Physiology 2021Quote: ... Peptides were first trapped (Dr. Maisch Reprosil C18, 3 μm, 2 cm × 100 μm) prior to separation on an analytical column (Agilent Poroshell EC-C18 ...
-
bioRxiv - Microbiology 2020Quote: ... Equal number of live cells was seeded at 2*105 cells per well in XF96 V3 PS plates (Seahorse Bioscience) precoated with 0.5 mg/ml Corning® Cell-Tack™ Cell and Tissue Adhesive (Corning ...
-
bioRxiv - Cell Biology 2021Quote: ... A Ddyo7-mCherry expression plasmid was generated by first TA cloning a PCR product (myo42 to myi185+2) encompassing the myoi 3’ region of the gene (aa 475 - end) minus the stop codon using StrataClone (Agilent) (pDTi289+2) ...
-
bioRxiv - Cell Biology 2021Quote: ... The membranes were then washed twice with TBS-T followed by incubation for 1 h at room temperature in immunoreaction enhancer solution 2 (Can Get Signal; TOYOBO) with peroxidase-conjugated antibody against rabbit IgG (Dako) as the secondary antibody ...
-
bioRxiv - Cell Biology 2021Quote: ... Cellular oxygen consumption was assessed in basal condition (prior to any addition) and after addition of oligomycin (2 μM; Agilent) Carbonyl cyanide-4 (trifluoromethoxy ...
-
bioRxiv - Cell Biology 2022Quote: ... 0.2% Triton X-100 and incubation with appropriate secondary antibody for 2 hours at room temperature in blocking buffer before washing and mounting in fluorescent mounting medium (DAKO). Images were acquired using a Leica SP8 confocal microscope.
-
bioRxiv - Microbiology 2022Quote: ... ORF6 mutations were introduced into the pLVX-StrepII-SARS-CoV-2-ORF6-IRES-Puro and pLVX-EF1α-SARS-CoV-2-ORF6-2xStrep-IRES-Puro plasmids using the QuickChange II-XL site-directed mutagenesis kit (Agilent) according to the manufacturer’s instructions using SDM primers listed below ...
-
bioRxiv - Biophysics 2022Quote: ... was pre-equilibrated with 10 mM pH 7 sodium phosphates with 150 mM NaCl on an Infinity 2 1260 HPLC system (Agilent) with an inline degasser ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples were filtered through Agilent Captiva syringe filters in to deactivated glass inserts (0.2 mL) placed inside amber glass vials (2 mL; Agilent Technologies). LC separations were carried out using a 1200 series LC system (Agilent Technologies) ...