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Citations for Agilent :
1801 - 1850 of 3726 citations for 2E 4E 2 4 Octadien 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 2 µg of the plasmid carrying the relevant recombinant provirus was co-transfected with 2 µg of plasmid pMD2.G (a kind gift from D. Trono) and 2 µg of plasmid PVPackgagpol (#217566 from Stratagene, La Jolla, CA) or plasmid pCMV-1′R8.74 (a gift from D ...
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bioRxiv - Microbiology 2023Quote: ... followed by sample measurements, all performed in duplicates using the OT-2 robot (Opentrons, Long Island City, NY) and BioTek Synergy (Agilent Technologies, Waldbronn, Germany). The acquired data were evaluated using the Shiny application based on the determined standard curves for accurate quantification [62].
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bioRxiv - Microbiology 2024Quote: ... The growth was monitored by measuring the optical density at 600 nm (OD600) in an Epoch 2 plate reader (Agilent, Santa Clara, CA). The growth rates were calculated using the R package growthcurver (32).
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bioRxiv - Neuroscience 2023Quote: The AAV plasmid of human synapsin I promoter (hSyn)-tetracycline-controlled transactivator 2 (tTA2) was constructed by subcloning the DNA fragments containing hSyn and tTA2 into pAAV-MCS (Agilent Technologies, CA, USA). The AAV vector was produced as described previously (Ebina et al. ...
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bioRxiv - Microbiology 2023Quote: ... Plate was shaken continuously at 30°C and OD600 was read every 20 minutes in a BioTek Epoch 2 Microplate Spectrophotometer (Agilent, Santa Clara, CA). MMC (0.5 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... The AAV plasmid of human synapsin I promoter (hSyn)-tetracycline-controlled transactivator 2 (tTA2) was constructed by subcloning the DNA fragments containing hSyn and tTA2 into pAAV-MCS (Agilent Technologies, CA, USA). The AAV vector was produced as described previously15,35,55 ...
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Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... Coverslips were then incubated with 1X DAPI for 5 min before mounting on slides using DAKO mounting media (Agilent Technologies, Cat #S302380-2). Images were captured at 60X or 100X using a Nikon C2+ confocal microscope coupled to NIS-Elements AR software (Nikon).
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bioRxiv - Neuroscience 2023Quote: ... Antigen □ retrieval was performed by incubating the tissue for 2 h at 70 °C in Target Retrieval Solution (Dako, Santa Clara, CA, USA) using a 1:10 dilution in demineralized water ...
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bioRxiv - Plant Biology 2023Quote: ... Ten-μl B314-2 sample was injected into an Eclipse XDB-C18 column (250 mm x 4.6 mm, 5 μm, Agilent, Santa Clara, CA, USA) and eluted with the same gradient solvent system described above ...
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bioRxiv - Cancer Biology 2022Quote: ... The antigen retrieval step was repeated followed by second round staining for pan-CK using anti-pan-cytokeratin antibody (AE1/AE3 mouse monoclonal, Dako-Cat# M351501-2) followed by HRP-conjugated anti-mouse secondary antibody polymer (EnVision+ ...
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bioRxiv - Pathology 2024Quote: The samples were resuspended in 10µL phase A of Q-TOF (2% ACN, 0.1% FA in water HPLC grade) in mass spectrometry polypropylene vials (Agilent technologies, reference 5190-3155). The samples (1µL out of 10µL ...
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bioRxiv - Biochemistry 2024Quote: ... Assay plates were generated by plating 2 µL of the deep well stock plates into 384-well assay plates (Thermo Scientific #142761 using the Agilent V11 Bravo). 88 µL of SrEpac cultured in high-nutrient media (5% Sea Water Complete ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.4 U/μl Murine RNase inhibitor (Vazyme, Cat. No. R301-03) and 2% AffinityScript Multiple Temperature Reverse Transcriptase (Agilent, Cat. No. 600100-51). After incubation of 5 min at 65°C of RNA with random hexamers ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plates were incubated at room temperature for 30 minutes and FRET signals (665 and 620 nm) were read using Biotek Synergy Neo 2 plate reader (Agilent, Santa Clara, CA). HTRF signal was calculated as the ratio of signal from the 665 nm (acceptor ...
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bioRxiv - Bioengineering 2024Quote: ... centrifuged at 1000 x g for 5 minutes and resuspended in 2 mL of assay media (Seahorse XF base medium without phenol red (Agilent, Cat. # 103334-100), 1 mM pyruvate (Acros Organics ...
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bioRxiv - Bioengineering 2024Quote: ... samples were treated with 0.3% Sudan black solution for 10 min prior to blocking for 2 h (Dako Blocking Solution X0909; Agilent Technologies, Santa Clara, CA). Immunohistochemical staining was then performed using antibodies to detect laminin (dilution 1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... 2×105 neutrophils were resuspended in 50 µL neutrophil mitochondria assay media containing Seahorse Agilent RPMI media (Agilent Technologies, Cat. #103576-100, US), 1 mM pyruvate ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-E-cadherin (diluted 1:100) antibody (Ab) and anti-vimentin Ab (1:1000) were purchased from DakoCytomation, mouse anti-p65 Ab (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... then followed by immunoblotting for ≤ 1 h with secondary antibodies: 1:5,000 anti-mouse linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Microbiology 2022Quote: ... then followed by immunoblotting for ≤ 1 h with secondary antibodies: 1:5,000 anti-mouse linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Immunology 2020Quote: ... followed by the incubation with horseradish peroxidase-labeled secondary antibody (1:10,000) for 1 h at room temperature (DAKO EnVision™+ System ...
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bioRxiv - Neuroscience 2021Quote: ... WAKO) antibodies were diluted at 1:250 and 1:1000 respectively with EnVision FLEX Antibody Diluent (K800621, Dako-Agilent) and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... WAKO) antibodies were diluted at 1:250 and 1:1000 respectively with EnVision FLEX Antibody Diluent (K800621, Dako-Agilent) and incubated overnight at 4°C ...
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bioRxiv - Pathology 2020Quote: ... It was then incubated with horseradish peroxidase-labelled secondary antibodies (cat. no. sc-2020; 1:5,000; Santa Cruz Biotechnology, Inc.; and cat. no. K4003; 1:5,000; Dako; Agilent Technologies ...
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bioRxiv - Pathology 2020Quote: ... It was then incubated with horseradish peroxidase-labelled secondary antibodies (cat. no. sc-2020; 1:5,000; Santa Cruz Biotechnology, Inc.; and cat. no. K4003; 1:5,000; Dako; Agilent Technologies ...
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bioRxiv - Genetics 2020Quote: ... We determined efficiency of each reaction using the equation Efficiency = −1+10(−1/slope of standard curve) (Agilent Genomics). We additionally calculated ratios of transcript abundance to determine expression levels of one gene relative to another gene and to account for any differences in underlying mRNA levels among individuals ...
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bioRxiv - Microbiology 2020Quote: ... The MUF production (fmol MUF cell-1 h-1) was measured with a Cary Eclipse spectrofluorometer (FL0902M009, Agilent Technologies) at 359 nm (excitation ...
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bioRxiv - Cancer Biology 2022Quote: ... was followed by incubation with streptavidin conjugated to horseradish peroxidase (1:200; 1.25% NGS/1% BSA in PBS; 30 min) (#P0397, DakoCytomation). For visualization DAB (#D5905 ...
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bioRxiv - Neuroscience 2024Quote: ... anti-Gfap rabbit polyclonal antibody (1:2,000 [brain] or 1:4,000 [spinal cord]; Z0334; Dako/Agilent, Santa Clara, CA); and anti-Iba1 rabbit polyclonal antibody (1:2,000 [brain] or 1:4,000 [spinal cord] ...
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bioRxiv - Neuroscience 2024Quote: ... anti-Gfap rabbit polyclonal antibody (1:2,000 [brain] or 1:4,000 [spinal cord]; Z0334; Dako/Agilent, Santa Clara, CA); and anti-Iba1 rabbit polyclonal antibody (1:2,000 [brain] or 1:4,000 [spinal cord] ...
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bioRxiv - Neuroscience 2023Quote: ... followed by washing in TBST (0.1%) and a 1 h incubation in secondary antibody (goat a-mouse-HRP: 1:10000, P0447, Dako). After washing ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating brain slices were incubated in a solution of anti-Iba1 antibody (1:2000; Fuji film-Wako) or anti-GFAP antibody (1:10,000; Agilent-Dako) at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... The following day slides were rinsed and incubated with horseradish peroxidase labelled secondary antibodies for 1 hour and developed using 3,3′-diaminobenzidine (DAB, 1:50, DAKO) for 5 minutes ...
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bioRxiv - Genetics 2022Quote: The wild-type and mutant BRD-1-BRC-1 chimeras were expressed in BL21-CodonPlus (DE3)-RIPL cells (Agilent). The cells were grown at 37°C until OD600 0.6 and were induced by 0.2mM isopropyl-β-D-thiogalactoside in the presence of 100μM ZnCl2 at 37°C for 6 hrs ...
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bioRxiv - Microbiology 2024Quote: ... Each culture was then diluted at 1:500 to 1:1,000 in TSB or Seahorse XF DMEM (Agilent Technologies) supplemented with 100 mM glucose ...
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bioRxiv - Immunology 2024Quote: ... Permaebilisation/block buffer was removed and samples were incubated with primary antibodies α-Lysozyme 1 (1:40, DAKO, A0099), α-DCAMKL1 (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... prior to incubation in primary antibodies (anti-YKL-40, Abcam ab77528, 1:1,000; anti-C3d, Agilent A0063, 1:1,000). Sections were incubated in primary antibodies for one hour at room temperature prior to washing and probing with the universal probe and polymer-HRP components of the Mach 4 Polymer kits (CellPath ...
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bioRxiv - Systems Biology 2024Quote: ... for 1 h and washed in PBS before being incubated with anti-rat biotinylated secondary antibody (Dako, 1:100) for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... the same membrane was also hybridized with an anti-mouse GAPDH antibody (1:2,000, Novus IMG3073) and with a secondary anti-goat IgG HRP-conjugated antibody (1:2,000, Dako P0449). Immunoblots were visualized by ECL Chemiluminescent analysis system ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were incubated with anti-tubulin (Thermo PA1-41331; dilution 1:250) and K9JA (Dako A0024; dilution 1:500) antibody ...
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bioRxiv - Cell Biology 2020Quote: ... then probed for 1 hr at room temperature with HRP-conjugated goat anti-mouse IgG secondary antibodies 1:500 in PBS (Dako, Agilent Technologies ...
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bioRxiv - Systems Biology 2020Quote: ... After double XP beads purifications (with beads added in a ratio 1:1) libraries were quantified on a Fragment Analyzer run with a NGS Assay Kit (Agilent) and loaded on a HiSeq 3000 flowcell with 50 cycles single end sequencing by pooling the samples based on molarity aiming for 30 mio ...
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bioRxiv - Neuroscience 2020Quote: ... Briefly, Iba1 (Wako, 019-19741, 1:2,000) was used to visualize microglia/macrophages and anti-GFAP (Dako, Z0334, 1:15,000) was used to visualize astrocytes ...
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bioRxiv - Immunology 2021Quote: ... rabbit anti-VWF (A0082, 1:1000) and rabbit anti-VWF-HRP (P0226, 1:8000) used for sandwich ELISA were purchased from Dako, sheep anti-VWF (ab11713 ...
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bioRxiv - Cancer Biology 2020Quote: ... trifluoroacetamide containing 1% trimethylchlorosilane (Pierce, 20 μl, 1 hour, 37 °C) and analysed by GC-MS using a VF5 capillary column (Agilent, 30 m ...
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bioRxiv - Microbiology 2020Quote: ... The blocked membrane was then probed with anti-HA (1:1000 dilution, CellSignalling) and rabbit anti-mouse P260 IgG conjugated with HRP (1:1000 dilution, DAKO). The antibody-stained membrane was then soaked with SuperSignal West Pico PLUS substrate (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK; 1:800) in Antibody Diluent (S0809, Agilent DAKO). Following removal of excess primary antibody with wash buffer ...
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bioRxiv - Microbiology 2021Quote: ... Nucleus staining was performed with DAPI 1 µg/mL for 1 min and coverslips were mounted on slides with fluorescent mounting medium (Dako). Cells were observed under a SP8 confocal laser-scanning microscope equipped with an HCP PL APO 100x/1.44 Oil CORR CS immersion objective (Leica) ...
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bioRxiv - Neuroscience 2020Quote: ... Immunohistochemistry with polyclonal antibody 1175 (1:1,000) directed against α-synuclein phosphorylated at Ser129 or anti-ubiquitin (DAKO, 1:10,000) were performed as described previously (Masuda-Suzukake et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted 1:100 or CD3 antibodies (Spring biosciences, Clone SP7, ref. M3070) diluted 1:200 in Antibody diluent solution (DakoCytomation). After several washes in PBS ...