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Citations for Agilent :
1751 - 1800 of 2370 citations for 5 4 Iodophenoxy methyl furan 2 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: 250 M10M6-PtenC124S cells and 750 M10M6-PtenWT cells were seeded in 384 well plate with 40 µL RPMI-1640 medium containing 5% FBS using Bravo automated liquid handling platform (Agilent). After 24 hours ...
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bioRxiv - Plant Biology 2023Quote: ... 10 μg of TMT-labeled peptides were loaded onto 10 cm capillary columns packed with 5 μM Zorbax SB-C18 (Agilent), which was connected using a zero dead volume 1 μm filter (Upchurch ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were then transferred immediately into a 5 mm NMR tube (Wilmad) and placed into a 600 MHz magnet with a coldprobe (Agilent). The peptide backbone was assigned using a combination of BEST versions of 3D HNCA ...
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bioRxiv - Microbiology 2023Quote: ... The optical density was measured at 540 nm and subtracted from readings at 450 nm using the BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). Concentrations were calculated from standard curves run in parallel.
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bioRxiv - Systems Biology 2023Quote: ... the peptides were reconstituted in 10 mM TEAB and fractionated using a bRPLC column (Agilent 300 Extend-C18 column, 5 µm, 4.6 mm × 250 mm, Agilent Technologies) under an increasing gradient of the mobile phases consisting of 10 mM TEAB in water and 90% acetonitrile (ACN) ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed in PBS and incubated in DAPI (1:500) for 5 min before mounting with the immunofluorescence mounting media (DAKO).
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bioRxiv - Microbiology 2023Quote: ... a mutant version of each of the hsdR1 had a unique BamHI site inserted by site directed mutagenesis of a C at position 1033 of hsdR1 to a G using oligonucleotide directed mutagenesis (5’-CATCAGAGACTTTTTTAGCGGATCCAACCTAAACAAAAAGAC-3’) (Quick-Change Lightning. Agilent). The mutated site is shown in bold and italics and the generated BamHI site is underlined ...
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bioRxiv - Microbiology 2023Quote: ... Membrane fluidity was determined by using an excitation wavelength of 350 nm and measuring fluorescence at 460 nm and 500 nm emission wavelengths on a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). The generalised polarisation (GP ...
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bioRxiv - Microbiology 2023Quote: ... 200 μl were aliquoted into black 96 well plates in triplicate and fluorescence was measured using a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). The excitation wavelength used was 488 nm and emission wavelength used was 530 nm.
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bioRxiv - Bioengineering 2023Quote: ... slides were counterstained with DAPI (1µg/ml) in PBS for 5 min followed by water washes and cover slipping with fluorescent antifade mounting reagent (DAKO, Agilent).
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bioRxiv - Bioengineering 2023Quote: ... Peptides were loaded to a nanoscale HPLC column composed of 10 cm of Polaris C18 5 μm packing material (Agilent), followed by 4 cm of Partisphere 5 SCX (Whatman) ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed 3 times (5 min) in TBST prior to addition of secondary antibody (goat anti-rabbit HRP 1:10,000; Dako - P00448). Blots were washed 3 times in TBST prior to development using SuperSignal West Pico Plus substrate (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... Mixtures were incubated at RT for 60 min in the dark and absorbance at OD570 was measured on a microplate reader (Bio Tek Cytation 5, Agilent). For each LD sample ...
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bioRxiv - Immunology 2024Quote: ... Sections were blocked with 5% horse serum in 1% BSA solution and incubated with the anti-CD68 primary antibody (KP1, Dako) for 60 minutes at room temperature ...
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bioRxiv - Genomics 2024Quote: ... each library underwent a reconditioning PCR to reduce heteroduplexes: libraries were concentrated down to 5 µl and mixed with 10 µl of Herculase Buffer (Agilent), 5 µl of 2.5 nM dNTPs ...
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bioRxiv - Plant Biology 2024Quote: ... The plates were placed in either a BioTek Cytation 5 or Synergy HT plate reader (Agilent, Santa Clara, CA, USA) set at 22°C ...
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bioRxiv - Cell Biology 2024Quote: ... The HPLC conditions employed two normal-phase Zorbax Sil (5 μm, 4.6 × 150 mm) columns (Agilent, Santa Clara, CA, USA), connected in series within the Multicolumn Thermostat compartment ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: ... cell nuclei were stained with DAPI for 5 min prior to mounting the cover slip onto a glass slide using DAKO mounting medium (Agilent). Cells were visualized through 63x ...
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bioRxiv - Cancer Biology 2024Quote: ... 2,000 cells were seeded on a well of a 96-well plate and brightfield images were taken every two hours by using the BioTek BioSpa 8 Automated Incubator and Citation 5 reader (Agilent). BioTek Gen5 imaging software (Agilent ...
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bioRxiv - Immunology 2024Quote: ... We performed the HPLC with a normal phase Zobax Sil (5 μm, 4.6 × 150 mm) column (Agilent, Santa Clara, CA). Isocratic chromatographic separation was achieved with 10% ethyl acetate/hexane at a flow rate of 1.4 ml/min ...
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bioRxiv - Microbiology 2024Quote: The archived samples of DNA extracts were tested for endogenous KoRV-5’ using an AriaMx real-time PCR system (Agilent) with recKoRV_200 primers under the optimised conditions (see results) ...
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bioRxiv - Physiology 2024Quote: ... interfaced to a nanoscale reversed-phase high-pressure liquid chromatography (Tempo) with a 10 cm-180 micron ID glass capillary packed with 5-µm C-18 ZorbaxTM beads (Agilent) as described before 37.
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bioRxiv - Molecular Biology 2024Quote: Serial sections of human and mouse FFPE BM biopsies were prepared at 3-5 μm thickness on coated microscope slides (Dako FLEX, Agilent) and processed for immunohistochemistry/immunofluorescent-based (IHC-IF) ...
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bioRxiv - Biochemistry 2024Quote: ... A Zorbax SB-C8 reversed-phase column (5 μm, 2.1 x 50 mm) was obtained from Agilent (Palo Alto, CA). The LC eluent was introduced into the ESI source of the mass spectrometer ...
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bioRxiv - Neuroscience 2024Quote: ... sound stimuli consisted in single-cycle sound pips (5-ms duration at 200 Hz) produced by a function generator (33210A Agilent Technologies Inc. ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: ... This construct was also used to generate the let-7 ʻseed’ deletion mutant derivative using the QuikChange Multi Site Mutagenesis Kit (catalog 200514-5, Stratagene). 3T3 mouse embryonic fibroblasts (MEFs ...
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bioRxiv - Molecular Biology 2024Quote: ... equipped with a DB-5 column (inner diameter, 0.25 mm; length, 30 m; film thickness, 1 μm; #122-5033, Agilent Technologies). Helium was used as carrier gas ...
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bioRxiv - Molecular Biology 2024Quote: ... with the shRNA target site (5’-TGGAGAACTTGGAAATGGAA) in PCNA disrupted by site-directed mutagenesis using the Quick Change system (Stratagene). Stable cell lines expressing HA-PCNA-WT or HA-PCNA-K164R were generated by lentiviral infection ...
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bioRxiv - Plant Biology 2024Quote: ... and 1.5 μl of this dilution was added to 5 μl of qRT-PCR Brilliant III SYBR Master Mix (Agilent Technologies). Quantitative Real-time PCR (qRT-PCR ...
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bioRxiv - Biophysics 2024Quote: ... a ∼5 V cm-1 electric field was manually applied across the compartment using a DC power supply (E3620A, Agilent) to pull the chromosome into the bottom capillary ...
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bioRxiv - Biochemistry 2024Quote: Analytical SEC was conducted on a Nexera Bio HPLC (Shimadzu) using a Bio SEC-5 2000 Å pore size column (7.8 × 300 mm, Agilent) with a Bio SEC-5 2000 Å guard column (7.8 × 50 mm ...
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bioRxiv - Biochemistry 2024Quote: ... were kept in a 5°C autosampler prior to analysis and injected onto a reverse- phase Zorbax SB-C18 column (150×3 mm, 5 μm particle size, Agilent, Santa Clara ...
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bioRxiv - Cell Biology 2024Quote: ... FITC-dextran levels were measured (ex: 485 nm/em: 528nm) based on a standard curve on an Biotek Cytation 5 (Agilent) microplate reader ...
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bioRxiv - Cancer Biology 2024Quote: ... and imaged with the 4x objective every 12 h for 10-14 days using a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). Images were processed for data acquisition using BioTek GenV software (Agilent) ...
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bioRxiv - Cell Biology 2024Quote: ... Luciferin Detection reagent was added to all wells and luciferase readings were taken using a BioTek Cytation 5 (Agilent Technologies) plate reader.
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bioRxiv - Cancer Biology 2024Quote: ... with 0.1% v/v Ammonium Hydroxide (Alfa Aesor) in 90% Water (Honeywell)/10% Acetonitrile (BioSolv) containing 5 µM Infinitylabs Deactivator Solution (Agilent). Solvent B was 90% Acetonitrile / 10% Water containing 5 µM Infinitylabs Deactivator Solution ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies (Supplementary Table 2) were diluted according to manufacturer’s guidelines in DAKO Antibody Diluent (cat. S202230, Agilent). Cells were incubated with the primary antibody mixes overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... we generated a pT7-D6/2-NSP1-null via the QuikChange II site-directed mutagenesis kit (Agilent Technology) based on pT7-D6/2-NSP1 (22) ...
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bioRxiv - Biochemistry 2020Quote: ... followed by horseradish peroxidase (HRP)-conjugated anti-mouse antibodies (Cat. N. P044701-2, Agilent Technologies LTD, Cheadle, UK) (2.4μg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... The tissues were deparaffinized to distilled water and the antigen retrieval performed with Proteinase K (Dako #S302030-2) treatment for 2 minutes ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR reactions contained 10 μL of 2×Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent), 2 μL of each 2 μM primers (Table S5) ...
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bioRxiv - Plant Biology 2021Quote: ... 2 µl aliquot of the sample was injected into LC-QTOF-MS system (Agilent, 6545 LC/QTOF-MS) coupled with a C18 column (ZORBAX RRHD Plus C18 ...
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bioRxiv - Plant Biology 2021Quote: ... All mutations listed in Supplemental Table 2 were introduced using the QuikChange Multi Site-Directed Mutagenesis Kit (Agilent) with appropriate mutagenic primers ...
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bioRxiv - Plant Biology 2021Quote: ... Purified peptide suspensions (2 μL each) were injected into a HPLC-chip (Polaris-HR-Chip-3C18, Agilent Technologies) through a capillary pump with a flow at 1.5 μL/min ...
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bioRxiv - Microbiology 2021Quote: ... and the quality was checked on 2% agarose gel and verified using the Agilent Bioanalyser 2100 (Agilent technologies). RNAs selected for further analysis had a minimum RNA Integrity Number (RIN ...
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bioRxiv - Cancer Biology 2022Quote: ... the slices were incubated with primary antibodies: rabbit polyclonal anti-PSMA/GCPII (1:150) (M362029-2; DAKO, US), anti-CD68 (1:250 ...
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bioRxiv - Microbiology 2022Quote: ... Sections for SARS-CoV-2 IHC were blocked with 10% goat serum (X0907, DAKO, Agilent Technologies Netherlands B.V) for 30 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... Sections for SARS-CoV-2 IHC were blocked with 10% goat serum (X0907, DAKO, Agilent Technologies Netherlands B.V) for 30 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... coli Lgt fused to a C-terminal Flag-tag was transformed into Rosetta 2(DE3) Gold cells (Agilent). Starter cultures were grown in Terrific Broth (TB ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-ALG-2 was obtained by performing a reverse mutagenesis (Quick change II site directed mutagenesis kit, Stratagene) on a GFP-hALG-2 Y180A (a generous gift from Masatoshi Maki ...