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Citations for Agilent :
1601 - 1650 of 6919 citations for Nitrite Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... using HS D1000 screentape kit (Agilent) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... and RNA 6000 Nano Kit (Agilent) before processing with the Ambion® WT Expression Kit and Affymetrix GeneChip Whole Transcript Sense Target Labeling Assay and hybridization to the Affymetrix Exon 1.0 ST Arrays following the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... Mitostress assay was performed according to Khan et al.42 using the Seahorse XFe24 analyzer (Agilent Technologies Inc. CA, USA). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... Thermal stability at a constant 37°C was measured by a thermal shift assay using a Mx3000p real-time PCR instrument (Agilent technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... and RNA quality was measured on a 2100-Bioanalyser using an RNA Nano Assay Chip (Agilent Technologies, Santa Clara, CA). Only samples with an RNA integrity number (RIN ...
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bioRxiv - Cell Biology 2022Quote: ... The quality and concentration of the extracted material was assessed using the RNA assay on a 2200 TapeStation (Agilent Technologies): all the samples had a RIN score higher than 8 ...
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bioRxiv - Developmental Biology 2022Quote: RNA was extracted with the NucleoSpin RNA extraction kit (Machery-Nagel) and sample quality and quantity were verified with the B2100 BioAnalyzer Instrument and Bioanalyzer RNA 6000 Nano assay (Agilent Technologies ...
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bioRxiv - Immunology 2020Quote: ... Generated data were normalized to viable cell number in million/ml at the viability was found to be consistently between 96% and 99% as determined by trypan blue exclusion assay (ViCell, Beckmann Coulter) and analyzed using Seahorse Glycolysis Stress Test Generator (Agilent).
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bioRxiv - Molecular Biology 2019Quote: ... Sample input was 900ng total RNA (determined by Qubit RNA HS reagents, Thermo) with a RIN >8 (TapeStation High Sensitivity RNA ScreenTape Assay, Agilent). RNA was fragmented for 15 minutes and libraries were constructed with a 1/5th dilution of NEB adaptor and 12 cycles of PCR amplification with dual-indexing primers ...
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bioRxiv - Immunology 2019Quote: ... An estimated 150 STAMPS (based on encapsulation frequencies and bead counts) were taken further for library prep (High Sensitivity DNA Assay, Agilent Bioanalyser ...
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bioRxiv - Genomics 2019Quote: ... The size and quality of each final library were validated on an Agilent 2100 Bioanalyzer with the DNA 7500 assay (Agilent). Libraries were sequenced using TruSeq SBS Kit v3-HS in paired end mode with the read length 2×76bp ...
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bioRxiv - Genomics 2020Quote: ... Library sizes and concentrations were measured using a genomic DNA assay on the TapeStation 4200 (Agilent Technologies, Santa Clara, CA). Tumor DNA was fragmented to a target size of 200 bp on E220 Focused-ultrasonicator (Covaris ...
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bioRxiv - Genomics 2021Quote: ... ADT and HTO library sizes and estimated concentrations were determined in 2200 TapeStation System High Sensitivity D1000 assays (Agilent Technologies). Qubit™ dsDNA HS assays (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Integrity was verified by agarose gel electrophoresis (1.5%, 100 V, 45 min; Carl Roth) and Bioanalyzer measurements (RNA 6000 Nano Assay, Agilent Technologies).
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bioRxiv - Evolutionary Biology 2020Quote: ... and assessed fragment length using a High Sensitivity DNA Assay on an Agilent 2100 Bioanalyzer (Agilent Technologies; Santa Clara, CA). We generated DNA libraries using a KAPA Biosystems Hyper Prep kit (Wilmington ...
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bioRxiv - Molecular Biology 2020Quote: ... The purity and quality of isolated RNA were assessed by RNA 6000 Nano assay on a 2100 Bioanalyzer (Agilent Technologies). Total RNA from each sample (250 ng ...
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bioRxiv - Neuroscience 2020Quote: ... Concentration and cDNA size was analyzed for selected representative wells using a High Sensitivity D5000 assay for the Tapestation 4200 system (Agilent). cDNA was diluted to an average of 200 pg/µl and 100 pg cDNA from each cell and was tagmented using Nextera XT DNA Library Preparation Kit (Illumina) ...
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bioRxiv - Cancer Biology 2021Quote: ... all DNA samples were evaluated for concentration by fluorometric Qubit assays (Thermo-Fisher) and for integrity by TapeStation (Agilent Technologies). Sequencing libraries were prepared using the TruSeq DNA PCR-free HT sample preparation kit from Illumina ...
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bioRxiv - Microbiology 2022Quote: ... and subsequently pooled in equimolar amounts prior to fragment analysis on 4200 TapeStation System using the D5000 ScreenTape Assay (Agilent). The final pooled library was sequenced an Illumina MiSeq using a V3 flowcell and 600 cycle kit (Illumina).
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bioRxiv - Systems Biology 2022Quote: ... In both cases we checked library size distribution via tapestation using D1000 (RNA) and D5000 assays (ATAC) respectively on a Tapestation4200 instrument (Agilent) and quantified the libraries via Qubit HS dsDNA assay (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: ... Quality of submitted RNA samples and the resulting cDNA libraries was determined by ScreenTape Assay on a 4200 TapeStation (Agilent). Prepared libraries were submitted for single-end 75 bp sequencing on an Illumina HiSeq 4000 (Illumina).
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bioRxiv - Molecular Biology 2021Quote: ... Size-distribution of cDNA libraries was measured using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system (Agilent). cDNA libraries were quantified using KAPA Library Quantification Kits (Kapa Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA integrity was measured using the High Sensitvity RNA ScreenTape Assay in an Agilent TapeStation (Agilent Technologies, Inc.; Fig. S1). The RNA sample (40ng ...
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bioRxiv - Neuroscience 2021Quote: ... Mitochondrial oxygen consumption rate (OCR) was measured by extracellular flux (XF) assay (Seahorse XFp analyzer, Agilent Technologies, Santa Clara, CA), according to manufacturer’s procedures ...
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bioRxiv - Cell Biology 2022Quote: ... and extracellular acidification rates (ECAR) were measured in XF DMEM assay medium with pH adjusted to 7.4 (103680-100, Agilent Technologies) containing 10 mM glucose (103577-100 ...
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bioRxiv - Developmental Biology 2022Quote: ... The size of sequencing libraries was determined by means of High Sensitivity D5000 Assay in at Tapestation 4200 system (Agilent). Pooled indexed libraries were then sequenced on the Illumina HiSeq X platform with 150-bp paired-end reads.
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bioRxiv - Cell Biology 2022Quote: ... The quality control of cDNA and obtained final libraries was performed using a Bioanalyzer High Sensitivity DNA Analysis assay (Agilent). Library quantification was performed using the Collibri™ Library Quantification Kit (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2022Quote: ... viral protein in the virus-infected cells was detected by ELISA assay using anti-SARS-CoV-2 nucleocapsid mAb (40143-R001, SinoBiological) and HRP-conjugated goat anti-rabbit pAb (P0448, Dako). After 10 min incubation with TMB substrate ...
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bioRxiv - Cancer Biology 2022Quote: ... and its quality was evaluated with the RNA 6000 Nano Assay on a Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA). Libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep Kit protocol (Illumina ...
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bioRxiv - Biochemistry 2019Quote: ... Assays were resolved by SDS-PAGE and results were analysed by western blotting with anti-ubiquitin primary antibody (Z0458, Dako) and anti-rabbit HRP secondary antibody for the unlabelled Ubc13~Ub conjugate ...
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bioRxiv - Molecular Biology 2020Quote: ... The size-distribution of cDNA libraries was measured using the Agilent high sensitivity D5000 assay on a Tapestation 4200 system (Agilent). cDNA libraries were quantified using a Qubit high sensitivity dsDNA assay ...
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bioRxiv - Cancer Biology 2020Quote: ... the cell media was replaced with the assay media and ran using the Seahorse XF Cell Mito Stress Test and ATP Rate Assay on a Seahorse XFe96/XF96 analyzer (Agilent). Hoechst 33342 staining (ThermoFisher ...
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bioRxiv - Synthetic Biology 2021Quote: ... Concentration and cDNA size was checked for select representative wells using a High Sensitivity DNA5000 assay for the Tapestation 4200 (Agilent). cDNA was diluted to an average of 200 pg/µl and 100 pg cDNA from each cell was tagmented by adding 1 µl TD and 0.5 µl ATM from a Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Fragment size and quality was assessed by the Agilent TapeStation 4200 using the High-Sensitivity DNA ScreenTape Assay (Agilent, USA) on TruSeq input DNA pre- and post-sonication ...
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bioRxiv - Immunology 2019Quote: ... Concentration and cDNA size were checked for select representative wells using a High Sensitivity DNA5000 assay for the Tapestation 4200 (Agilent). cDNA was diluted to an average of 200pg/μl and 100pg cDNA from each cell was tagmented by adding 1μl TD and 0.5μl ATM from a Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Genetics 2020Quote: ... ribosome profiling library size distributions were checked on the Bioanalyzer 2100 using a high sensitivity DNA assay (Agilent; 5067-4626), multiplexed and sequenced on an Illumina HiSeq 2500 producing single end 1×51nt reads ...
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bioRxiv - Plant Biology 2021Quote: ... DNA contamination and first quantification were performed via RNA Pico Assay for the 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA). Quantification was verified via a Qubit RNA BR (Broad-Range ...
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bioRxiv - Microbiology 2021Quote: ... Mass validation of peptide products from in vitro cleavage assays or in vivo endogenous production was performed by LC-MS/MS on a 6546 LC/Q-TOF (Agilent) employing a 1290 Infinity II LC system using an Atlantis dC18 column (5 μm ...
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bioRxiv - Microbiology 2021Quote: ... A second clean up with magnetic beads was performed and the libraries were quantified using the ThermoFishrer High Sensitivity dsDNA Qubit assay and validated using the TapeStation (Agilent) with the DNA100 screentape assay ...
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bioRxiv - Cell Biology 2020Quote: ... followed by washes in buffered Seahorse Assay medium (pH 7.4) and subsequent metabolic testing according to the manufacturer’s protocol (Agilent Seahorse XF96). Oligomycin (2 μM ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... Library concentration was determined using Qubit and average fragment size was estimated using DNA HS assay on bioanalyzer 2100 (Agilent) before pooling libraries at equimolar ratio ...
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bioRxiv - Neuroscience 2021Quote: ... Size and concentration of the RNA-seq libraries was measured by the Agilent 2100 Bioanalyzer using the DNA 1000 assay (Agilent) before loading onto the sequencer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we quantified the libraries using Qubit DNA Assay and assessed the fragments using DNA TapeStation D1000 Screen Tape (#5067-5582,5583, Agilent Technologies). We finally clonally amplified the quantified libraries on a cBOT and sequenced them on the HiSeq X with 150bp paired end chemistry.
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bioRxiv - Developmental Biology 2020Quote: ... Quality of submitted RNA samples and the resulting cDNA libraries was determined by ScreenTape Assay on a 4200 TapeStation (Agilent). Prepared libraries were submitted for single-ended 75 bp sequencing on an Illumina HiSeq 4000 (Illumina).
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bioRxiv - Cancer Biology 2021Quote: Metabolic flux assays measuring oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were performed using the Seahorse XFe96 Analyzer (Agilent). Patient-derived GBM cells were seeded at a density of 1 to 1.5 × 104 cells per well in NSC medium in laminin-coated Seahorse XFe96 cell culture microplates ...
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bioRxiv - Microbiology 2020Quote: ... The integrity of the RNA was checked both in a 1.2% agarose gel and with RNA6000 Nano Assay using the Agilent 2100 Bioanalyzer (Agilent Technologies).
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bioRxiv - Microbiology 2021Quote: ... and concentration of each IVT standard were confirmed using the Agilent Bioanalyzer RNA 6000 Nano assay (Agilent, Santa Clara, CA) prior to dilution in nuclease-free water to working concentrations.
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bioRxiv - Genomics 2021Quote: ... Final library concentration was assessed by Qubit dsDNA HS Assay Kit (Thermo-Fischer Scientific) and fragment size was checked using the High Sensitivity D1000 ScreenTape assay on a Tapestation 4200 (Agilent) to ensure that fragment sizes were distributed normally about 500 bp ...
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bioRxiv - Genomics 2021Quote: ... Final library concentration was assessed by Qubit dsDNA HS Assay Kit (Thermo-Fischer Scientific) and fragment size was inspected using the High Sensitivity D1000 ScreenTape assay on a Tapestation 4200 (Agilent). Libraries were sequenced using a NextSeq 500 or NovaSeq 6000 (Illumina ...
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bioRxiv - Genomics 2021Quote: ... The cfDNA concentration was determined using an Agilent 4200 TapeStation System with the Cell-free DNA ScreenTape Analysis assay (Agilent).