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Citations for Agilent :
1551 - 1600 of 3795 citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... The staining was performed according to the manufacturer’s procedure with EnVision G|2 Doublestain System Rabbit/Mouse (DAB+/Permanent Red) kit (Dako/Agilent K5361) on the Dako Autostainer ...
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bioRxiv - Bioengineering 2021Quote: ... or rabbit anti-CD206 antibody for 60 mins at room temperature Secondary antibodies donkey anti-mouse HRP and donkey anti-rabbit HRP (Dako) were applied for 30 mins at RT and samples were exposed DAB substrate (Dako ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBS + 0.1% Tween 20 and 5% dried skimmed milk powder and probed with relevant primary antibodies followed by horseradish peroxidase (HRP)-conjugated polyclonal goat anti-mouse and polyclonal goat anti-rabbit secondary antibodies (Dako). Membranes were treated with EZ-ECL (Geneflow ...
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bioRxiv - Cancer Biology 2021Quote: ... for 30 min or with 0.4 µg/ml anti-human cath-D mouse monoclonal antibody (clone C-5) for 20 min after heat-induced antigen retrieval with the PTLink pre-treatment (Dako) and the High pH Buffer (Dako ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were resolved on 10–16% gradient SDS-PAGE under reducing conditions followed by immunoblotting for PMCA (primary antibody: 5F10 anti-pan-PMCA, Abcam, ab2825, secondary antibody: anti-mouse-HRP, Dako) and aSN (primary antibody ...
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bioRxiv - Pathology 2022Quote: ... appropriate HRP-coupled secondary antibodies (rabbit anti-mouse, Cat. No. P0260, and goat anti-rabbit, Cat. No. P0448, from DAKO and rabbit anti-goat ...
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bioRxiv - Molecular Biology 2022Quote: ... sections were incubated for 30 minutes in DAKO Envision-Plus System HRP Labelled Polymer Anti-Mouse (DAKO, Catalog No. K4001). Peroxidase labeling was visualized with the Liquid DAB+ Substrate Chromogen System (DAKO ...
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bioRxiv - Cancer Biology 2022Quote: ... For signal detection the MACH 3 Mouse HRP Polymer Detection system was employed according to manufacturer’s protocol using DAB+ (Fa.Agilent Technologies, K3468). Slides were counter-stained with Hematoxylin Gill’s Formula (Fa.Vector ...
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bioRxiv - Immunology 2020Quote: ... membranes were incubated with secondary Abs (goat anti-rabbit HRP or rabbit anti-mouse HRP; DAKO, Agilent Technologies, Heverlee, Belgium) for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by incubation with the Dako REALTM EnvisionTM-HRP Rabbit/Mouse system and substrate development was done with DAB (DAKO). Scanning of the slides was done using the Zeiss Axio Scan.Z1 (Zeiss ...
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bioRxiv - Cancer Biology 2020Quote: Microarray was performed on in vitro cultures of dMMR and CIN MC38 CRC cells using the SurePrint G3 Mouse Gene Expression v2 Microarray (8×60K) (Agilent) system ...
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bioRxiv - Immunology 2020Quote: ... Cytospots were stained by May-Gruenwald Giemsa stain and mast cells were detected immunohistochemically (monoclonal mouse anti-human mast cell tryptase, Dako). Staining protocols and further details can be found in the supplementary material ...
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bioRxiv - Cancer Biology 2020Quote: ... The secondary antibodies used for western blotting were horseradish peroxidase (HRP)-conjugated antibody against mouse immunoglobulin G (IgG) and (HRP)-conjugated antibody against rabbit IgG from Dako.
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bioRxiv - Developmental Biology 2021Quote: Microarray analysis was performed on six control and six IL1B samples (O4+ cells isolated at P5 after in vivo PBS or IL1B treatment) using Agilent Whole Mouse Genome Oligo Microarrays 8×60K (Agilent). Raw data are available in Dataset S2 ...
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bioRxiv - Cancer Biology 2020Quote: ... and then with the primary (Supplementary Table 2) (overnight 4°C) and the secondary (HRP-conjugated anti-mouse or -rabbit, DAKO) (2h ...
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bioRxiv - Genomics 2020Quote: ... The library preparations for the genome-wide data were performed according to the SureSelect XT Mouse Methyl-Seq Kit Enrichment System for Illumina Multiplexed Sequencing Library protocol (Agilent Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... horse radish peroxidase (HRP)-conjugated anti-mouse IgG pAb (P0260) and anti-rabbit IgG pAb (P0448)(Dako, Carpinteria, CA, USA). Proteins were separated by SDS-PAGE system and transferred to Immobilon-P membrane (Merck KGaA ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed thrice in PBS and incubated for 30 min with HRP-conjugated rabbit anti-mouse antibody (DaKo, P0161) diluted 1:5000 in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... After washing with TBST three times, membranes were exposed to appropriate secondary antibodies (anti-rabbit, anti-mouse, and anti-rat) coupled to HRP (Dako) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were washed and incubated with the corresponding secondary antibodies when needed (rabbit anti mouse, Abcam; rabbit anti rat, Vector; Goat anti-rabbit, Dako) and visualization systems (Bond Polymer Refine Detection ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was washed 3 times 15 minutes with TBST before a 1h incubation of horseradish peroxidase (HRP)-conjugated antibodies (P0447 goat anti-mouse IgG HRP, Dako, 1:2500 or P0399 swine anti-rabbit IgG HRP ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated for 1h at RT with the secondary antibody (Polyclonal Goat Anti-Rabbit Immunoglobulins/HRP, Dako; Polyclonal Goat Anti-Mouse Immunoglobulins/HRP, Dako) prepared in the same blocking solution at a dilution of 1:10.000 ...
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bioRxiv - Neuroscience 2022Quote: ... For PrP deposition anti-PrP monoclonal antibodies ICSM3525,26 were used in conjunction with biotinylated polyclonal rabbit anti-mouse immunoglobulin secondary antibodies (Dako; Agilent) and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit ...
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bioRxiv - Molecular Biology 2022Quote: ... This was followed by incubation with the appropriate anti-rabbit or anti-mouse IgG antibody conjugated to horseradish peroxidase (Dako) for 60 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were washed twice with PBS-Tween and incubated for 30 minutes at room temperature with the Rabbit/Mouse EnVision kits (K4001/K4007, Dako). Slides were counterstained with hematoxylin and ...
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Treatment with furosemide indirectly increases inhibitory transmission in the developing hippocampusbioRxiv - Neuroscience 2023Quote: ... The membrane was washed 3 times 15 minutes with TBST before a 1h incubation of horseradish peroxidase (HRP)-conjugated antibodies (P0447 goat anti-mouse IgG HRP, Dako, 1:2500 or P0399 swine anti-rabbit IgG HRP ...
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bioRxiv - Biochemistry 2024Quote: ... The presentation of epitopes of the BinJV/WNVKUN when applied to the membrane was confirmed using a goat anti-mouse Ig (Dako) conjugated gold nanoparticle (AuNP ...
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bioRxiv - Neuroscience 2023Quote: ... RNA integrity of brain organoids and mouse tissue was evaluated by analysis on the 2100 Bioanalyzer RNA 6000 Pico Chip kit (Agilent) using the RNA Integrity Number (RIN) ...
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bioRxiv - Cancer Biology 2023Quote: ... Staining visualisation was performed by incubating with a cocktail of anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Envision Kit, Agilent) for 60 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: Anti-PrP monoclonal antibody ICSM35 (D-Gen Ltd) was used with biotinylated polyclonal rabbit anti-mouse immunoglobulin secondary antibodies (Dako; Agilent) and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit ...
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bioRxiv - Immunology 2023Quote: ... sorted neutrophils were added to CMT-93 (mouse colonic epithelial cells; ATCC) monolayer on 96-well E-plate (Agilent # 300601010), and changes in TEER were measured every 15 minutes for up to 72hrs as described previously (71 ...
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bioRxiv - Biochemistry 2023Quote: The pET-30a-PHB1 vector encoding His-tagged mouse PHB1 was transformed into Escherichia coli strain BL21 Star (DE3; Stratagene). The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare) ...
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bioRxiv - Cancer Biology 2023Quote: ... libraries were constructed and sequenced similarly to the WGS experiment following hybrid capture selection with the hybrid reagent SureSelect DNA - Mouse All Exon V1 (Agilent). WES reads were aligned to the GRCm38 reference genome using BWA-MEM ...
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bioRxiv - Immunology 2023Quote: DNA whole exome libraries of B16-F10 cell line and C57BL/6JOlaHsd germline sample were prepared with Agilent Mouse All Exon kit (Agilent) following manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...
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bioRxiv - Cancer Biology 2024Quote: ... Proliferating cells were immunostained with FLEX monoclonal mouse anti-human Ki-67 (Catalog No. GA626, Ready-to-use (Dako Omnis), Clone MIB-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The tissue was washed twice in TBS-T prior incubation with secondary antibody (EnVision anti-mouse-HRP/DAB+ system, Agilent), 3,3′-Diaminobenzidine (DAB ...
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bioRxiv - Biochemistry 2024Quote: ... sections were incubated for 30 min at RT with the detection system (Envision+ System HRP Mouse and Rabbit, respectively; Agilent Dako), followed by incubation with diaminobenzidine as chromogen and counterstaining with hematoxylin.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were washed 3 times with 500 µL PBS before the addition of the secondary antibody (HRP-conjugated anti-mouse IgG (1.5mg/mL) (Dako, Denmark)) diluted 1:2000 in 1% BSA PBS for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: Serial sections of human and mouse FFPE BM biopsies were prepared at 3-5 μm thickness on coated microscope slides (Dako FLEX, Agilent) and processed for immunohistochemistry/immunofluorescent-based (IHC-IF) ...
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bioRxiv - Immunology 2024Quote: ... Immunodetection was carried out with the indicated antibodies (Extended Data Table 2) and bound antibodies were visualized with peroxidase-conjugated affinity-purified donkey anti-mouse or anti-rabbit IgG (Dako), or HRP-linked anti-rat IgG (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were stained for hematoxylin & eosin and immunohistochemistry was performed with antibodies for Pan-Cytokeratin (Pan-CK) (MNF116, mouse; Agilent), Ionized Calcium-Binding Adaptor molecule 1 (IBA-1 ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound antibody was removed with washing buffer and the fraction of cells with surface protein labeled with CD44 antibody was determined using a MoFlo cell sorter (Dako Cytomation).
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bioRxiv - Molecular Biology 2020Quote: ... All samples were depleted of the 7 most abundant proteins with the multiple affinity removal system (MARS Hu7 spin cartridge) according to the manufacturers protocol (Agilent Technologies). Samples were further reduced using 4 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: Zyxin and Pxl1 LCR proteins were expressed in Escherichia coli strain BL21-Codon Plus (DE3)-RP (Agilent Technologies, Santa Clara, CA) with 0.5 mM isopropyl β-D-1-thiogalactopyranoside for 16 h at 16 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were prepared according to the instruction of the Agilent protein 80 kit and analyzed on a 2100 Bio-analyzer device (Agilent technologies). The ATPase activity of FSS-PscN was determined by measuring the release of total phosphate at 37°C for 30 minutes which was detected by the malachite green method (Casabona et al. ...
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bioRxiv - Immunology 2020Quote: ... followed by a quick rinse in TBS before being returned to the black chamber to be incubated with serum free protein block (Dako X0909) for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of 2 – 4 mg/ml protein samples were applied to a column using the 1260 Infinity HPLC system (Agilent Technologies) coupled to a MiniDawn Treos detector (Wyatt Technologies ...
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bioRxiv - Immunology 2022Quote: ... brucei Heat Shock Protein 70 (TbHSP70) (Kind gift from Professor Keith Matthews, Edinburgh, UK) using a Dako Autostainer Link 48 (Dako, Denmark) and were subsequently counterstained with Gill’s haematoxylin.
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bioRxiv - Biochemistry 2022Quote: ... Proteins were detected using tryptophan fluorescence (λex=280 nm; λem=315 nm) using a fluorescence detector (Agilent technologies 1200 series, G1321A). The peak heights of the monomeric hsFPN peaks were used to assess the stability by normalizing heights to the corresponding value from samples incubated at 4 °C (100% stability) ...