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Citations for Agilent :
1551 - 1600 of 2610 citations for Mouse Anti Tick borne Encephalitis Virus NS1 M838 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Gene expression analysis was conducted using Agilent Whole Mouse Genome 4×44 multiplex format oligo arrays (Agilent Technologies 014868) following the Agilent 1-color microarray-based gene expression analysis protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Isolated mouse cardiomyocytes were seeded at 2K per well in the Seahorse XF96 cell culture microplates (Agilent Technologies, 102416100) pre-coated with laminin mouse protein at 2μg/cm2 (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... The bisulfite-converted library was PCR amplified and indexed using the SureSelect XT Mouse Methyl-Seq Kit (Agilent, #G9651A). Prepared libraries were run on an Illumina sequencing platform using a NextSeq High 150 bp paired-end mode.
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bioRxiv - Cell Biology 2019Quote: ... washed in PBS before saturation with 0.6 μg/mL mouse IgG2 diluted in Dako Antibody Reducing Background buffer (Dako) for 30 min ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA obtained from FACS-sorted E7.5 mouse embryonic populations was purified with the Absolutely RNA nanoprep kit (Agilent) and quantified as above ...
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bioRxiv - Bioengineering 2021Quote: ... Serotec) mouse monoclonal antibodies, or Aβ40 (1:200, Covance), Iba-1 (1:200, Wako) and GFAP (1:1000, DAKO) rabbit polyclonal antibodies ...
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bioRxiv - Pathology 2023Quote: ... three clusters of lysine- and arginine-residues and one cluster of asparagine residues were exchanged for alanine within mouse Prnp in a pcDNA3.1 vector by site-directed mutagenesis (QuikChange site-directed mutagenesis kit; Agilent). PrP-deficient RK13 cells (ATCC ...
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bioRxiv - Neuroscience 2023Quote: ... followed by washing in TBST (0.1%) and a 1 h incubation in secondary antibody (goat a-mouse-HRP: 1:10000, P0447, Dako). After washing ...
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bioRxiv - Developmental Biology 2024Quote: ... at a density of 9.1 × 105 cells/cm2 (mouse assay) or 7.3 × 105 cells/cm2 (human assay) in 100 μl of Seahorse XF DMEM (Agilent) supplemented with glucose (20 mM) ...
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bioRxiv - Neuroscience 2019Quote: ... with 0.3% TritonX-100 for 1 hour at room temperature and then incubated overnight at 4°C with the primary antibodies rabbit anti-Iba-1 (Wako: 019-19741, RRID:AB_839504) at 1:500 or rabbit anti-GFAP (Dako: Z0334, RRID: AB_10013382) at 1:1000 and then secondary antibodies (Alexa goat-647 anti-Rabbit ...
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bioRxiv - Cell Biology 2019Quote: ... anti-H4K20me2 The following secondary antibodies were used for western blot experiments: peroxidase-conjugated goat anti-rabbit (P448 DAKO, 1:2000) and goat anti-mouse (P447 DAKO ...
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bioRxiv - Microbiology 2020Quote: ... ACE2 was detected using a rabbit polyclonal anti-hACE2 antibody (Cell Signalling, 4355S; citrate antigen-retrieval) and EnVision+ anti-rabbit HRP (Agilent, K4003). Slides were scanned with a bright field slide scanner (Leica ...
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bioRxiv - Cancer Biology 2022Quote: ... For secondary antibody staining, either ImmPRESS kits (anti-goat, MP-7405; anti-rat, MP-7444) or Envision+ Reagents (Dako: rabbit, K4002) were used ...
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bioRxiv - Immunology 2022Quote: Tissue sections were then incubated with anti-PD1 (1:300, 3G8) and anti-PDL1 (1:100, 1F8) in antibody diluent (Dako, S0809) for 60 min (26) ...
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bioRxiv - Cell Biology 2021Quote: ... then primary antibody (anti-SOX9 at 1/200 with 0.1% TritonX-100, ABCAM, Cat # ab185966; anti-KRT19 at 1/100, DAKO Cat # M0888) diluted in diluent (DAKO ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation for 45 minutes at room temperature in 1:100 dilution of rabbit anti-goat immunoglobulins/HRP (Dako, P0160 or ready-to-use goat anti-rabbit HRP labelled polymer (Dako, K4003). The slides were washed twice in 1x Wash buffer and the color was developed with 3,3-diaminobenzidine tetrahydrochloride (DAB ...
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bioRxiv - Neuroscience 2023Quote: ... Microglia and Astrocyte proliferation was visualized by staining the brain sections using anti Iba1 (1:2500; WAKO) and anti GFAP antibody (1:1000 Agilent technologies). When appropriate the brain sections were counterstained with haematoxylin and eosin ...
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bioRxiv - Molecular Biology 2023Quote: ... The reporter cell lines were stained with anti-human CD45 and anti-human CD19 antibodies and analyzed by flow cytometry on an ACEA NovoCyte 2060 (Agilent, USA). ACEA NovoExpress (Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated with primary antibodies (chicken anti-Map2 [Thermo Scientific, PA1-16751, 1:1000] and rabbit anti-Gfap [Dako, Z0334 ...
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bioRxiv - Genomics 2021Quote: ... the sections were incubated with an anti-ESR1 antibody (FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α, Agilent technologies, Dako, Glostrup, Denmark) at a 1:2 dilution for 60 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... the sections were incubated with an anti-ESR1 antibody (FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α, Agilent technologies, Dako, Glostrup, Denmark) at a 1:2 dilution for 60 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Consecutive slides were incubated with anti-ATM (1:100) or anti-SMA (1:200) diluted in Dako REAL Antibody Diluent (Dako, Glostrup, Denmark). Slides were then treated with biotinylated secondary antibodies and target staining was performed with peroxidase-conjugated streptavidin and DAB chromogen (Dako REAL Detection System Peroxidase/DAB+ ...
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bioRxiv - Cancer Biology 2021Quote: ... immunohistochemical staining was performed using an antibody directed against the mouse endothelial cell surface molecule CD31 (JC70A, DAKO, Glostrup, Denmark). 10 μm-thick cryosections were fixed with 4% PFA solution for 20 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... mouse MLE12 cells were seeded at a concentration of 20,000 cells/well on XFp cell plates (Seahorse Bioscience, 103057-100) 24 hours prior to the initiation of studies ...
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bioRxiv - Cell Biology 2020Quote: The Bub1 K795R allele was generated by a single-base substitution in full-length mouse Bub1 cDNA cloned into pcDNA3/Myc using site-directed mutagenesis (Stratagene), then subcloned into a pShuttleCMV vector as a BglII-NotI digest ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated with primary or with negative isotype control mouse IgG2a (cat. X0943 - Agilent Technologies, Santa Clara, CA, USA) or negative isotype control rabbit IgG (cat ...
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bioRxiv - Immunology 2022Quote: ... for 1 hour RT and replacing the secondary antibody incubation with HRP-labeled polyclonal goat α-mouse antibody (Dako K4000) for 30 minutes RT ...
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bioRxiv - Genomics 2019Quote: ... Library preparation and target enrichment was carried out using the SureSelect XT Mouse All Exon Kit (Agilent cat.# 5190-4641) per manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Whole genome expression data was obtained using Agilent Sureprint G3 Mouse Gene Expression 8×60K microarrays (Agilent Technologies cat.# G4852B) with Low Input Quick Amp Labeling protocol (Agilent Technologies cat.# 5190-2331 ...
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bioRxiv - Systems Biology 2021Quote: ... The staining was performed according to the manufacturer’s procedure with EnVision G|2 Doublestain System Rabbit/Mouse (DAB+/Permanent Red) kit (Dako/Agilent K5361) on the Dako Autostainer ...
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bioRxiv - Cancer Biology 2021Quote: ... for 30 min or with 0.4 µg/ml anti-human cath-D mouse monoclonal antibody (clone C-5) for 20 min after heat-induced antigen retrieval with the PTLink pre-treatment (Dako) and the High pH Buffer (Dako ...
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bioRxiv - Cancer Biology 2022Quote: ... For signal detection the MACH 3 Mouse HRP Polymer Detection system was employed according to manufacturer’s protocol using DAB+ (Fa.Agilent Technologies, K3468). Slides were counter-stained with Hematoxylin Gill’s Formula (Fa.Vector ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by incubation with the Dako REALTM EnvisionTM-HRP Rabbit/Mouse system and substrate development was done with DAB (DAKO). Scanning of the slides was done using the Zeiss Axio Scan.Z1 (Zeiss ...
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bioRxiv - Cancer Biology 2020Quote: Microarray was performed on in vitro cultures of dMMR and CIN MC38 CRC cells using the SurePrint G3 Mouse Gene Expression v2 Microarray (8×60K) (Agilent) system ...
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bioRxiv - Developmental Biology 2019Quote: ... Candidate Onecut1 cis-regulatory elements were amplified from chick or mouse genomic DNA with Herculase II polymerase (Agilent, 600677-51), treated for 10-30 minutes with Taq polymerase (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... The secondary antibodies used for western blotting were horseradish peroxidase (HRP)-conjugated antibody against mouse immunoglobulin G (IgG) and (HRP)-conjugated antibody against rabbit IgG from Dako.
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bioRxiv - Developmental Biology 2021Quote: Microarray analysis was performed on six control and six IL1B samples (O4+ cells isolated at P5 after in vivo PBS or IL1B treatment) using Agilent Whole Mouse Genome Oligo Microarrays 8×60K (Agilent). Raw data are available in Dataset S2 ...
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bioRxiv - Genomics 2020Quote: ... The library preparations for the genome-wide data were performed according to the SureSelect XT Mouse Methyl-Seq Kit Enrichment System for Illumina Multiplexed Sequencing Library protocol (Agilent Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... RNA integrity of brain organoids and mouse tissue was evaluated by analysis on the 2100 Bioanalyzer RNA 6000 Pico Chip kit (Agilent) using the RNA Integrity Number (RIN) ...
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bioRxiv - Immunology 2023Quote: ... sorted neutrophils were added to CMT-93 (mouse colonic epithelial cells; ATCC) monolayer on 96-well E-plate (Agilent # 300601010), and changes in TEER were measured every 15 minutes for up to 72hrs as described previously (71 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cytokeratin 5/6 (mouse monoclonal) at 1:100 to 1:200 dilution for zebrafish tissue and 1:100 dilution for human tissue (Dako), GATA 3 (SAB2100898 ...
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bioRxiv - Biochemistry 2023Quote: The pET-30a-PHB1 vector encoding His-tagged mouse PHB1 was transformed into Escherichia coli strain BL21 Star (DE3; Stratagene). The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare) ...
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bioRxiv - Cancer Biology 2023Quote: ... libraries were constructed and sequenced similarly to the WGS experiment following hybrid capture selection with the hybrid reagent SureSelect DNA - Mouse All Exon V1 (Agilent). WES reads were aligned to the GRCm38 reference genome using BWA-MEM ...
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bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...
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bioRxiv - Cancer Biology 2023Quote: ... Patient-matched primary tumor tissues and PDTOs were immunostained for diagnostic NSCLC markers with antibodies against cytokeratin (CK)7 (mouse, M7018, 1:100; Dako), CK5/6 (mouse ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were washed twice with PBS-Tween and incubated for 30 minutes at room temperature with the Rabbit/Mouse EnVision kits (K4001/K4007, Dako). Slides were counterstained with hematoxylin and ...
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bioRxiv - Immunology 2023Quote: DNA whole exome libraries of B16-F10 cell line and C57BL/6JOlaHsd germline sample were prepared with Agilent Mouse All Exon kit (Agilent) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... sections were incubated for 30 min at RT with the detection system (Envision+ System HRP Mouse and Rabbit, respectively; Agilent Dako), followed by incubation with diaminobenzidine as chromogen and counterstaining with hematoxylin.
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bioRxiv - Neuroscience 2024Quote: Cells were counted and plated at 1 × 105 (mouse astrocytes or neurons) cells per well in a Seahorse XF24 Cell Culture Microplate for all experiments (Agilent). Cells were then treated with indicated inhibitors or agonists in each experiment for 20 h ...
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bioRxiv - Bioengineering 2024Quote: ... The tissue was hybridized with an antibody against CD138/Syndecan-1 (clone MI15, mouse, #M7228, Agilent, Santa Clara, CA, USA), following standard procedures by automated processing using the Tecan Evo Freedom stainer and the Dako Advance system according to the manufacturer’s protocol.