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Citations for Agilent :
1551 - 1600 of 1944 citations for 7 Chloro 3 4 dihydro 1H quinoxalin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... GFP-ALG-2 was obtained by performing a reverse mutagenesis (Quick change II site directed mutagenesis kit, Stratagene) on a GFP-hALG-2 Y180A (a generous gift from Masatoshi Maki ...
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bioRxiv - Genetics 2019Quote: ... We collected the supernatant and read 2 μl from each sample in the TapeStation (Agilent high sensitives D1000) to verify fragment size ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were incubated with appropriate primary antibodies diluted in EnVision FLEX Antibody Diluent (Agilent Dako, K800621-2) overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were incubated with appropriate primary antibodies diluted in EnVision FLEX Antibody Diluent (Agilent Dako, K800621-2) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... and AlixΔPGY (AlixΔALG-2) cDNAs in pCI were generated by mutagenesis (Quick change II site directed mutagenesis kit, Stratagene) and Alix R757E (AlixΔendo ...
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bioRxiv - Genomics 2020Quote: The Aβ coding sequence and two flanking regions of 52bp and 72bp respectively upstream and downstream of Aβ were amplified (primers MS_01 and MS_02, Supplementary file 2) by error-prone PCR (Mutazyme II DNA polymerase, Agilent). 30 cycles of amplification and 0.01ng of initial template were used to obtain a mutagenesis rate of 16 mutations/kb ...
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bioRxiv - Genomics 2020Quote: ... 1 - 2 ng / µl was examined using a 2100 Bioanalyzer and a corresponding High Sensitivity DNA Kit (Agilent) to determine the MW profile of the size-selected library ...
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bioRxiv - Immunology 2021Quote: A pAAV-MCS plasmid containing inverted terminal repeats (ITRs) from AAV serotype 2 (Agilent Technologies, Santa Clara, CA) was utilized as backbone for AAV6 plasmid construction (naturally occurring AAV6 has an AAV2 ITR (24)) ...
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bioRxiv - Cell Biology 2021Quote: ... T-cell lymphoma diagnoses were confirmed by immunohistochemical staining of sections with anti-CD3 antibody (Agilent, M725429-2) and DAB solution development.
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bioRxiv - Microbiology 2022Quote: ... pneumoniae was done in 96 well microtitre plates using a BioteK Epoch-2 microtiter plate reader (Agilent Technologies). Each treatment was replicated in at least 3 wells ...
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bioRxiv - Developmental Biology 2022Quote: ... Repeated washes were performed before incubation with secondary antibody (FITC-labelled swine anti-rabbit F (ab’)2 (Dako) or Alexa Fluor 647-labelled goat anti-rabbit IgG (Molecular Probes ...
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bioRxiv - Physiology 2022Quote: ... were placed in 50 µl XF medium (non-buffered DMEM containing 10 mM glucose and 2 mM GlutaMax, pH 7.3-7.4, Agilent), centrifuged in a carrier tray (300g for 3 min with no break) ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was then performed at 97 °C for 10 minutes with Target Retrieval Solution (Agilent, S236784-2) on a PT Module (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Growth curves for H53 wild type and ΔcirA strains were measured using BioTek Epoch 2 microplate reader with BioTek Gen6 v.1.03.01 (Agilent). Colonies of each strain were inoculated in 5 mL Hv-Cab liquid medium and grown shaking at 45°C until an OD600 0.15 was reached ...
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bioRxiv - Developmental Biology 2024Quote: ... 2009) (1μg) were co-transfected into H9 hESC (70,000 cells cm-2) using GeneJammer transfection reagent (Agilent Technologies). To enrich for successfully transfected cells ...
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bioRxiv - Immunology 2024Quote: ... sections were stained with monoclonal mouse anti-human CD68 clone PG-M1 - 1:100 (Dako Omnis: GA61361-2). Second antigen retrieval and blocking were performed as described above and subsequent staining was performed with either rabbit anti-Gas6 - 1:100 (PA5-79300 ...
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bioRxiv - Biochemistry 2024Quote: ... Headspace GC measurements were carried out on an Agilent 8890 gas chromatograph system equipped with a flame ionization detector (FID) and a Hayesep Q packed column (1.8 m x 2 mm x 3.17 mm) (Agilent) operating with an Argon carrier gas (flow rate= 5 mL/min) ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated for 20 minutes in a microwave oven in DAKO Target Retrieval pH6 (S203130-2, DAKO, Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... rabbit F(ab’)2 anti-human C1q-FITC (both at 5 µg/ml, Dako as described in (9)) ...
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bioRxiv - Bioengineering 2022Quote: ... before incubating in primary antibody diluted in DAKO antibody diluent (Cat# S080983-2, Agilent, Santa Clara, CA, USA) for 1 h ...
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bioRxiv - Immunology 2023Quote: ... the buffer was exchanged into basic PBS (pH 8.0) with 0.1 % Tween-20 for a 2-hour conjugation of 12.0 μM streptavidin (Prozyme, SA10) and 100 μM fluorescent dye (Alexa Fluor 488 Cadaverine or Lissamine Rhodamine B ethylenediamine ...
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bioRxiv - Cancer Biology 2023Quote: ... The slides were steamed for 35 minutes with a pH 6 Dako Target Retrieval (Agilent Technologies, S169984-2) and permeabilized with 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... transferred onto glass slides and mounted with a fluorescence anti-fading mounting medium (DAKO Fluoromount; #S302380-2, Agilent). In order to reduce age-related autofluorescence in human cortical slices ...
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bioRxiv - Neuroscience 2023Quote: ... transferred onto glass slides and mounted with a fluorescence anti-fading mounting medium (DAKO Fluoromount; #S302380-2, Agilent). In order to reduce age-related autofluorescence in human cortical slices ...
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bioRxiv - Cell Biology 2023Quote: ... at 5 mM for 2 h or by UV irradiation at 20 mJ/cm2 using Stratalinker 1800 (Stratagene) followed by 2 h incubation at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Primary antibodies against human CD3 (1:100; #A045229-2) and CD68 (1:400; #M0876) were obtained from Dako, UK ...
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bioRxiv - Microbiology 2024Quote: ... sections were blocked for 1 h at room temperature in serum-free blocking buffer (Agilent Technologies, X090930-2), and then incubated with polyclonal rabbit antibody to ASBT (kindly provided by Paul Dawson ...
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bioRxiv - Genetics 2024Quote: ... PCR-2 reactions amplified vector fragments from MSp509_PKR-WT plasmid using Herculase II polymerase (Agilent Technologies Cat#600677) using cycling conditions for vector targets >10 kb ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was harvested by centrifugation at 1500 rcf for 4 minutes at 23°C and filtered using a Captiva 96 well 0.2 μm filter plate (Agilent Technologies, Santa Clara, CA) prior to titer measurement by RP-UHPLC.
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bioRxiv - Molecular Biology 2021Quote: ... iPSC-derived kidney organoids were cut at a thickness of 4 μm using a cryo-microtome and mounted on FLEX IHC Microscope Slides (DAKO, Agilent Technologies, Amstelveen, Netherlands).
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bioRxiv - Genomics 2020Quote: ... and electroporated in 5 separate cuvettes containing 4 µL of the plasmid and 40 µL of ElectroTen-Blue electrocompetent cells (Agilent Santa Clara, CA). After a 1 hour outgrowth in LB at 37C ...
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bioRxiv - Bioengineering 2019Quote: ... Substrates and inhibitors were injected during measurements at a final concentrations of 3.5 μM 4-(trifluoromethoxy) phenylhydrazone (FCCP; Seahorse Bioscience, Billerica, MA, USA), 1 μM oligomycin ...
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bioRxiv - Biophysics 2021Quote: ... incubated for 16 h at 4 °C and purified by HPLC on a Zorbax C3 column with dimensions of 21 × 150 mm (Agilent, Santa Clara, CA) using a gradient of 20-90 % acetonitrile in water with 0.1 % trifluoroacetic acid (TFA ...
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bioRxiv - Neuroscience 2023Quote: ... Afterwards the sections were incubated in a humidified chamber (overnight 4°C) in a peroxidase conjugated immunoglobulin against UEA (DAKO, P289; 1:50). Finally ...
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bioRxiv - Cell Biology 2024Quote: Hybridization of samples was conducted on Agilent Whole Human Genome 4×44-k 60-mer oligonucleotide arrays (G4112F, Agilent Technologies, Santa Clara, CA), using 150ng of total RNA as template/sample ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Bioengineering 2022Quote: ... eye cups were post-fixed for 10 min in 4 % FA-PBS at RT following by flat-mounting with fluorescent mounting medium (Dako, Carpinteria, CA, USA). Fluorescence images were acquired with Nikon Eclipse Ti-E microscope (Nikon ...
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bioRxiv - Bioengineering 2024Quote: ... The solvent was then diluted with water (DMSO:water = 1:4) and subjected to purification through preparative reverse-phase HPLC chromatography (Agilent 1260 Infinity II HPLC). The chromatographic separation was carried out on an Agilent Prep-C18 column (250 mm × 21.2 mm × 10 μm ...
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bioRxiv - Neuroscience 2024Quote: ... they were incubated overnight at 4°C with the respective primary antibodies: (i) polyclonal rabbit anti-GFAP (1:2000; Dako, Cat. No. Z0334); (ii ...
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bioRxiv - Bioengineering 2024Quote: ... The solvent was then diluted with water (DMSO:water = 1:4) and subjected to purification through preparative reverse-phase HPLC chromatography (Agilent 1260 Infinity II HPLC). The chromatographic separation was carried out on an Agilent Prep-C18 column (250 mm × 21.2 mm × 10 μm ...
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bioRxiv - Bioengineering 2024Quote: ... The solvent was then diluted with water (DMSO:water = 1:4) and subjected to purification through preparative reverse-phase HPLC chromatography (Agilent 1260 Infinity II HPLC). The chromatographic separation was carried out on an Agilent Prep-C18 column (250 mm × 21.2 mm × 10 μm ...
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bioRxiv - Plant Biology 2021Quote: ... Collected volatiles were thermally desorbed for 3 min at 220°C and analyzed by GC-MS (Agilent 7820A GC interfaced with and Agilent 5977E MSD, Palo Alto, CA, USA). Desorbed volatiles were separated with split-less mode on a column (HP5-MS ...
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bioRxiv - Plant Biology 2020Quote: ... The excised fragment was introduced in the MCS 2 (EcoRI/NotI) of the yeast expression vector pESC-URA (Stratagene), cloned in the E ...
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bioRxiv - Bioengineering 2022Quote: ... SARS-CoV-2 RBD mutant plasmids were generated by Quikchange site-directed mutagenesis according to manufacturer’s instructions (Agilent, 210513). Biotinylated proteins were made by co-transfecting Avitagged RBD plasmids with a BirA expression plasmid and into Expi293 cells using FectoPRO ...
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bioRxiv - Microbiology 2019Quote: ... Immunolabelling was performed as follows: grids were placed directly on drops of 2% normal goat serum (DAKO, Glostrup, Denmark) in 0.1 M phosphate buffer to block non-specific binding then incubated with the primary antibodies ...
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bioRxiv - Plant Biology 2021Quote: ... Each sample set was run together with the external standard (2-AA labeled dextran ladder, Glyko Prozyme, Hayward, CA). In each chromatogram 15 individual peaks were identified and peak assignments were made basing on glucose units (GU) ...
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bioRxiv - Biophysics 2020Quote: ... They were then stained for 5 min with 4,6-diamidino-2-phenylindole (DAPI) and mounted using fluorescence mounting medium (DAKO, Agilent). Pictures were taken with an UltraVIEW ERS spinning disk confocal microscope (Zeiss 63X/1.4 ...
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bioRxiv - Bioengineering 2021Quote: ... samples were subjected to a treatment with 0.3% Sudan black solution for 10 min prior to blocking for 2 h (Dako Blocking Solution X0909 ...
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bioRxiv - Genetics 2020Quote: ... Step 1.3 of the protocol was performed with the following modifications: Step 1.3.q slides were incubated in Dako bluing buffer (#CS70230-2 Agilent) for 30 s ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and 10% Methanol for 10 min and incubated overnight with the primary antibody (Table 2) in PBS/T 0.1 with 10 % normal swine/ goat or rabbit serum (Dako). Second ...