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Citations for Agilent :
1501 - 1550 of 2118 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The plates were placed in either a BioTek Cytation 5 or Synergy HT plate reader (Agilent, Santa Clara, CA, USA) set at 22°C ...
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bioRxiv - Immunology 2024Quote: ... We performed the HPLC with a normal phase Zobax Sil (5 μm, 4.6 × 150 mm) column (Agilent, Santa Clara, CA). Isocratic chromatographic separation was achieved with 10% ethyl acetate/hexane at a flow rate of 1.4 ml/min ...
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bioRxiv - Cancer Biology 2024Quote: ... 2,000 cells were seeded on a well of a 96-well plate and brightfield images were taken every two hours by using the BioTek BioSpa 8 Automated Incubator and Citation 5 reader (Agilent). BioTek Gen5 imaging software (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... The HPLC conditions employed two normal-phase Zorbax Sil (5 μm, 4.6 × 150 mm) columns (Agilent, Santa Clara, CA, USA), connected in series within the Multicolumn Thermostat compartment ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: ... cell nuclei were stained with DAPI for 5 min prior to mounting the cover slip onto a glass slide using DAKO mounting medium (Agilent). Cells were visualized through 63x ...
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bioRxiv - Biochemistry 2024Quote: ... A Zorbax SB-C8 reversed-phase column (5 μm, 2.1 x 50 mm) was obtained from Agilent (Palo Alto, CA). The LC eluent was introduced into the ESI source of the mass spectrometer ...
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bioRxiv - Neuroscience 2024Quote: ... sound stimuli consisted in single-cycle sound pips (5-ms duration at 200 Hz) produced by a function generator (33210A Agilent Technologies Inc. ...
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bioRxiv - Molecular Biology 2024Quote: ... with the shRNA target site (5’-TGGAGAACTTGGAAATGGAA) in PCNA disrupted by site-directed mutagenesis using the Quick Change system (Stratagene). Stable cell lines expressing HA-PCNA-WT or HA-PCNA-K164R were generated by lentiviral infection ...
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bioRxiv - Biochemistry 2024Quote: Analytical SEC was conducted on a Nexera Bio HPLC (Shimadzu) using a Bio SEC-5 2000 Å pore size column (7.8 × 300 mm, Agilent) with a Bio SEC-5 2000 Å guard column (7.8 × 50 mm ...
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bioRxiv - Microbiology 2024Quote: The archived samples of DNA extracts were tested for endogenous KoRV-5’ using an AriaMx real-time PCR system (Agilent) with recKoRV_200 primers under the optimised conditions (see results) ...
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bioRxiv - Cell Biology 2024Quote: ... Mixtures were incubated at RT for 60 min in the dark and absorbance at OD570 was measured on a microplate reader (Bio Tek Cytation 5, Agilent). For each LD sample ...
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bioRxiv - Immunology 2024Quote: ... Sections were blocked with 5% horse serum in 1% BSA solution and incubated with the anti-CD68 primary antibody (KP1, Dako) for 60 minutes at room temperature ...
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bioRxiv - Genomics 2024Quote: ... each library underwent a reconditioning PCR to reduce heteroduplexes: libraries were concentrated down to 5 µl and mixed with 10 µl of Herculase Buffer (Agilent), 5 µl of 2.5 nM dNTPs ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: ... This construct was also used to generate the let-7 ʻseed’ deletion mutant derivative using the QuikChange Multi Site Mutagenesis Kit (catalog 200514-5, Stratagene). 3T3 mouse embryonic fibroblasts (MEFs ...
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bioRxiv - Molecular Biology 2024Quote: ... equipped with a DB-5 column (inner diameter, 0.25 mm; length, 30 m; film thickness, 1 μm; #122-5033, Agilent Technologies). Helium was used as carrier gas ...
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bioRxiv - Molecular Biology 2024Quote: Serial sections of human and mouse FFPE BM biopsies were prepared at 3-5 μm thickness on coated microscope slides (Dako FLEX, Agilent) and processed for immunohistochemistry/immunofluorescent-based (IHC-IF) ...
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bioRxiv - Physiology 2024Quote: ... interfaced to a nanoscale reversed-phase high-pressure liquid chromatography (Tempo) with a 10 cm-180 micron ID glass capillary packed with 5-µm C-18 ZorbaxTM beads (Agilent) as described before 37.
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bioRxiv - Biophysics 2024Quote: ... a ∼5 V cm-1 electric field was manually applied across the compartment using a DC power supply (E3620A, Agilent) to pull the chromosome into the bottom capillary ...
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bioRxiv - Biochemistry 2024Quote: ... were kept in a 5°C autosampler prior to analysis and injected onto a reverse- phase Zorbax SB-C18 column (150×3 mm, 5 μm particle size, Agilent, Santa Clara ...
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bioRxiv - Plant Biology 2024Quote: ... and 1.5 μl of this dilution was added to 5 μl of qRT-PCR Brilliant III SYBR Master Mix (Agilent Technologies). Quantitative Real-time PCR (qRT-PCR ...
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bioRxiv - Cell Biology 2024Quote: ... Luciferin Detection reagent was added to all wells and luciferase readings were taken using a BioTek Cytation 5 (Agilent Technologies) plate reader.
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bioRxiv - Cancer Biology 2024Quote: ... and imaged with the 4x objective every 12 h for 10-14 days using a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). Images were processed for data acquisition using BioTek GenV software (Agilent) ...
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bioRxiv - Cancer Biology 2024Quote: ... with 0.1% v/v Ammonium Hydroxide (Alfa Aesor) in 90% Water (Honeywell)/10% Acetonitrile (BioSolv) containing 5 µM Infinitylabs Deactivator Solution (Agilent). Solvent B was 90% Acetonitrile / 10% Water containing 5 µM Infinitylabs Deactivator Solution ...
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bioRxiv - Cell Biology 2024Quote: ... FITC-dextran levels were measured (ex: 485 nm/em: 528nm) based on a standard curve on an Biotek Cytation 5 (Agilent) microplate reader ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies (Supplementary Table 2) were diluted according to manufacturer’s guidelines in DAKO Antibody Diluent (cat. S202230, Agilent). Cells were incubated with the primary antibody mixes overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... we generated a pT7-D6/2-NSP1-null via the QuikChange II site-directed mutagenesis kit (Agilent Technology) based on pT7-D6/2-NSP1 (22) ...
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bioRxiv - Biochemistry 2020Quote: ... followed by horseradish peroxidase (HRP)-conjugated anti-mouse antibodies (Cat. N. P044701-2, Agilent Technologies LTD, Cheadle, UK) (2.4μg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... The tissues were deparaffinized to distilled water and the antigen retrieval performed with Proteinase K (Dako #S302030-2) treatment for 2 minutes ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR reactions contained 10 μL of 2×Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent), 2 μL of each 2 μM primers (Table S5) ...
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bioRxiv - Plant Biology 2021Quote: ... 2 µl aliquot of the sample was injected into LC-QTOF-MS system (Agilent, 6545 LC/QTOF-MS) coupled with a C18 column (ZORBAX RRHD Plus C18 ...
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bioRxiv - Plant Biology 2021Quote: ... All mutations listed in Supplemental Table 2 were introduced using the QuikChange Multi Site-Directed Mutagenesis Kit (Agilent) with appropriate mutagenic primers ...
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bioRxiv - Plant Biology 2021Quote: ... Purified peptide suspensions (2 μL each) were injected into a HPLC-chip (Polaris-HR-Chip-3C18, Agilent Technologies) through a capillary pump with a flow at 1.5 μL/min ...
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bioRxiv - Microbiology 2021Quote: ... and the quality was checked on 2% agarose gel and verified using the Agilent Bioanalyser 2100 (Agilent technologies). RNAs selected for further analysis had a minimum RNA Integrity Number (RIN ...
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bioRxiv - Cancer Biology 2022Quote: ... the slices were incubated with primary antibodies: rabbit polyclonal anti-PSMA/GCPII (1:150) (M362029-2; DAKO, US), anti-CD68 (1:250 ...
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bioRxiv - Microbiology 2022Quote: ... Sections for SARS-CoV-2 IHC were blocked with 10% goat serum (X0907, DAKO, Agilent Technologies Netherlands B.V) for 30 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... Sections for SARS-CoV-2 IHC were blocked with 10% goat serum (X0907, DAKO, Agilent Technologies Netherlands B.V) for 30 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... coli Lgt fused to a C-terminal Flag-tag was transformed into Rosetta 2(DE3) Gold cells (Agilent). Starter cultures were grown in Terrific Broth (TB ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-ALG-2 was obtained by performing a reverse mutagenesis (Quick change II site directed mutagenesis kit, Stratagene) on a GFP-hALG-2 Y180A (a generous gift from Masatoshi Maki ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were incubated with appropriate primary antibodies diluted in EnVision FLEX Antibody Diluent (Agilent Dako, K800621-2) overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were incubated with appropriate primary antibodies diluted in EnVision FLEX Antibody Diluent (Agilent Dako, K800621-2) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... and AlixΔPGY (AlixΔALG-2) cDNAs in pCI were generated by mutagenesis (Quick change II site directed mutagenesis kit, Stratagene) and Alix R757E (AlixΔendo ...
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bioRxiv - Genomics 2020Quote: The Aβ coding sequence and two flanking regions of 52bp and 72bp respectively upstream and downstream of Aβ were amplified (primers MS_01 and MS_02, Supplementary file 2) by error-prone PCR (Mutazyme II DNA polymerase, Agilent). 30 cycles of amplification and 0.01ng of initial template were used to obtain a mutagenesis rate of 16 mutations/kb ...
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bioRxiv - Genomics 2020Quote: ... 1 - 2 ng / µl was examined using a 2100 Bioanalyzer and a corresponding High Sensitivity DNA Kit (Agilent) to determine the MW profile of the size-selected library ...
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bioRxiv - Immunology 2021Quote: A pAAV-MCS plasmid containing inverted terminal repeats (ITRs) from AAV serotype 2 (Agilent Technologies, Santa Clara, CA) was utilized as backbone for AAV6 plasmid construction (naturally occurring AAV6 has an AAV2 ITR (24)) ...
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bioRxiv - Cell Biology 2021Quote: ... T-cell lymphoma diagnoses were confirmed by immunohistochemical staining of sections with anti-CD3 antibody (Agilent, M725429-2) and DAB solution development.
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated for 20 minutes in a microwave oven in DAKO Target Retrieval pH6 (S203130-2, DAKO, Agilent Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... 2009) (1μg) were co-transfected into H9 hESC (70,000 cells cm-2) using GeneJammer transfection reagent (Agilent Technologies). To enrich for successfully transfected cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was then performed at 97 °C for 10 minutes with Target Retrieval Solution (Agilent, S236784-2) on a PT Module (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Growth curves for H53 wild type and ΔcirA strains were measured using BioTek Epoch 2 microplate reader with BioTek Gen6 v.1.03.01 (Agilent). Colonies of each strain were inoculated in 5 mL Hv-Cab liquid medium and grown shaking at 45°C until an OD600 0.15 was reached ...
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bioRxiv - Immunology 2024Quote: ... sections were stained with monoclonal mouse anti-human CD68 clone PG-M1 - 1:100 (Dako Omnis: GA61361-2). Second antigen retrieval and blocking were performed as described above and subsequent staining was performed with either rabbit anti-Gas6 - 1:100 (PA5-79300 ...