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Citations for Agilent :
101 - 150 of 791 citations for Recombinant Human CD3d Molecule Delta CD3 TCR Complex His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... A single quadrupole mass spectrometer was used to determine m/z of sample molecules (Agilent, référence 5977B MSD). Molecules were identified using NIST libraries (NIST 2017 Mass Spectral Library).
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bioRxiv - Biochemistry 2019Quote: ... hexahistidine-tagged IN was overexpressed in BL-21 CodonPlus RIL cells (Agilent). Cells were lysed in 25 mM Tris-HCl pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... FLAG-tagged YTHDF1 was cloned into pCMV-Tag 4 vector (Agilent, #211174); Myc-tagged DDX60 was cloned into pRK-5 vector (BD PharMingen ...
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bioRxiv - Developmental Biology 2020Quote: ... Dual-tagged Slit2 Δ construct was obtained by Quickchange mutagenesis procedure (Agilent), deleting 9 amino acids (SPPMVLPRT ...
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bioRxiv - Biophysics 2022Quote: ... Recombinant plasmids were transformed in XL1Blue (Agilent Technologies) or Top10 (Thermofisher scientific ...
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bioRxiv - Immunology 2021Quote: ... Glycans were isolated and labeled with a fluorescent molecule using a GlykoPrep InstantAB labeling kit (Prozyme, Hayward, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and a 300×7.8 mm Hi-Plex Exchange column (Agilent, USA) was used to quantify glucose ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoreactions were visualized with a monoclonal APAAP-complex and a fuchsin-based substrate-chromogen system (Dako). Counterstaining was performed with Mayer’s hemalum (Merck) ...
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bioRxiv - Cancer Biology 2021Quote: ... immunohistochemical staining was performed using an antibody directed against the mouse endothelial cell surface molecule CD31 (JC70A, DAKO, Glostrup, Denmark). 10 μm-thick cryosections were fixed with 4% PFA solution for 20 min at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... solution (pH 7.4) and incubated with goat anti-mouse or anti-rabbit immunoglobulin labeled with dextran molecules and horseradish peroxidase (EnVision, Dako) at room temperature for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... solution (pH 7.4) and incubated with goat anti-mouse or anti-rabbit immunoglobulin labeled with dextran molecules and horseradish peroxidase (EnVision, Dako) at room temperature for 30 min ...
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bioRxiv - Genomics 2021Quote: ... and a second 1 μl aliquot was used to determine molecule length with an Agilent Genomic Tape (Agilent, Cheadle, UK) on an Agilent TapeStation (Agilent).
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bioRxiv - Genomics 2023Quote: ... The length distribution of extracted DNA molecules was assessed by electrophoresis on a TapeStation Genomic DNA ScreenTape assay (Agilent Technologies). Single-stranded DNA damage was treated with the NEBNext FFPE DNA Repair mix and repaired DNA was then subjected to size selection to remove fragments shorter than 40 kb using a PippinHT instrument (Sage Science ...
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bioRxiv - Neuroscience 2024Quote: ... Every fourth section from approximately bregma −1.40 mm to −2.00 mm were immunostained with ionized calcium binding adaptor molecule 1 (Iba1; 1:2000; FUJIFILM Wako) and glial fibrillary acidic protein (GFAP; 1:1000; Dako); 4-6 sections per brain ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues were blocked and incubated first with anti CD3 antibody (Dako, Supplementary Table 5) and subsequently with horseradish peroxidase conjugated anti rabbit antibody (Dako) ...
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bioRxiv - Immunology 2019Quote: ... Serial sections were stained with primary antibodies against CD3 (Dako A 40452, 1:200), TIGIT (Santa Cruz sc-103349 ...
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bioRxiv - Immunology 2019Quote: ... followed by staining with primary antibody (CD8 clone 4B11, Novocastra or CD3, polyclonal, Dako), secondary anti-mouse AP-conjugated antibody and incubation with substrate (Fast red chromogen ...
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bioRxiv - Biochemistry 2021Quote: ... N-terminally His6-tagged KRAS was expressed in BL21-CodonPlus(DE3)-RIL cells (Stratagene) by isopropyl-β-D-1-thiogalactopyranoside induction at 18 °C ...
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bioRxiv - Cell Biology 2022Quote: ... HA-tagged DCX mutant T203R were created using QuikChange Site-Directed Mutagenesis kit (Stratagene). HA-tagged DCX mutant A71S was synthesized commercially (Genewiz ...
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bioRxiv - Microbiology 2022Quote: OPG2-tagged ORF3c plasmids were generated by site-directed mutagenesis (Stratagene QuikChange, Agilent Technologies) and confirmed by DNA sequencing (GATC ...
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bioRxiv - Microbiology 2022Quote: OPG2-tagged ORF3c plasmids were generated by site-directed mutagenesis (Stratagene QuikChange, Agilent Technologies) and confirmed by DNA sequencing (GATC ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified from BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent, 230280) as follows ...
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bioRxiv - Cell Biology 2023Quote: ... the CBP-tagged Drp1 was purified by affinity chromatography using calmodulin agarose resin (Agilent) that had been pre-equilibrated with CalA Buffer ...
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bioRxiv - Biochemistry 2024Quote: ... the CBP-tagged Drp1 was purified by affinity chromatography using calmodulin agarose resin (Agilent) that had been pre-equilibrated with CalA Buffer ...
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bioRxiv - Immunology 2024Quote: ... C4b deposition was measured using a biotin-tagged anti-C4c polyclonal Ab (Agilent, Q0369) followed by HRP-streptavidin and TMB One ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA was extracted from four samples using a Qiagen Gentra HMW kit resulting in molecules with a length of mainly > 48 kbp (TapeStation, Agilent Genomics). Further work was carried out by The University of Liverpool’s Centre for Genomic Research ...
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bioRxiv - Biochemistry 2022Quote: ... The standard used in the analysis consisted of a mixture of glucose polymers ranging from a monomer to a polymer of 16 molecules (glucose1 - glucose16) (ProZyme, Inc.). Oligosaccharide bands in each sample were quantified by comparing the intensity of the glucose4 band (containing 25 pmols) ...
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bioRxiv - Microbiology 2023Quote: ... Identification of molecules was based on comparison with the metabolic libraries Agilent Fiehn 2013 GC-MS Metabolomics RTL (Agilent Technologies, USA) and the NIST17 Version 2.3 GC Method Retention Index Library ...
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bioRxiv - Cell Biology 2023Quote: ... solution (pH 7.4) and incubated with goat anti-mouse or anti-rabbit immunoglobulin labeled with dextran molecules and horseradish peroxidase (EnVision, Dako Omnis) at room temperature for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... the following primary antibody combinations were used: a) Rabbit anti-CD3 (1:50, Dako A0452), incubated overnight ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA synthesis was performed using Accuscript Hi Fidelity First Strand Synthesis kit (Agilent). The amount of RNA entered into the reaction was normalised between samples ...
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bioRxiv - Molecular Biology 2019Quote: pTrex-6×His-TOP1Y723F was generated by QuikChange II XL SDM kit (Agilent) using oligonucleotides 5’-CTAGGGTCCAGAAAATTGAGTTTGGAGGTTCCCAGG-3’ pTrex-6×His-TOP1 K117 ...
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bioRxiv - Developmental Biology 2019Quote: ... while the MURF1 cDNA fragment was cloned into myc-tagged pCMV-Tag3 (Stratagene, CA, USA). These constructs were sequenced to ensure that no errors were introduced.
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bioRxiv - Cell Biology 2019Quote: ... GST-tagged Nup153228-611 and Nup50 (full length) were purified from BL21-DE3 (Stratagene 230245) cells as described above.
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bioRxiv - Microbiology 2021Quote: ... The 5-AIQC-tagged samples (1 μL) were individually injected on an UPLC column (Agilent ZORBAX RRHD Eclipse XDB C18 column ...
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bioRxiv - Biophysics 2023Quote: Halo-tagged dCas9 protein expression was induced in BL21-CodonPlus(DE3)-RIL cells (Agilent #230245) transformed with pET302-6His-dCas9-halo (Addgene #72269 ...
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bioRxiv - Cancer Biology 2020Quote: ... Sections of human tumors were stained with mouse anti-human CD68 (Dako) and goat anti-human TSP-4 (AF2390 ...
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bioRxiv - Biochemistry 2023Quote: ... All recombinant plasmids were transformed into XL1-Blue competent cells (Agilent) and sequenced for verification.
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bioRxiv - Systems Biology 2019Quote: ... and human TTR (Dako) with standard curves of purified human RBP4 (72 ...
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bioRxiv - Cancer Biology 2021Quote: ... Human universal RNA (Agilent) was used as a reference to standardise results between QPCR batches.
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bioRxiv - Immunology 2019Quote: ... for 12h at 37°C before immunostaining according to standard protocol with anti-CD3 (Polyclonal; Dako), anti-CD8 (clone 4B11 ...
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bioRxiv - Bioengineering 2021Quote: ... Immune cell infiltrate was detected using rabbit polyclonal antibodies against CD3 (1:500, Dako, Cat#A0452), and Iba-1 (ionized calcium binding adaptor protein ...
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bioRxiv - Immunology 2019Quote: ... for 12h at 37°C before immunostaining according to standard protocol with anti-CD3 (Polyclonal; Dako), anti-CD4 (clone 1F6 ...
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bioRxiv - Microbiology 2022Quote: Primary polyclonal Rabbit Anti-CD3 antibody was used at a dilution of 1:200 (A0452, Dako) to mark T-cells as common player of adaptive immunity to intracellular agents in inflammatory infiltrates (Kapczynski and Segovia ...
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bioRxiv - Genomics 2019Quote: ... The concentration of the Hi-C libraries was determined by Bioanalyzer profiles (Agilent Technologies), and the Hi-C libraries were paired-end sequenced (HiSeq 2500 ...
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bioRxiv - Biochemistry 2021Quote: ... and the reaction was stopped by adding 2X GE HI-RPM hybridization buffer (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... We extracted dissolved organic molecules from the filtrate using solid phase extraction (SPE) with a modified styrene-divinylbenzene polymer (Agilent Bond Elut PPL) as a substrate ...
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bioRxiv - Genomics 2023Quote: ... The mean size of the library molecules and the quality of the libraries were determined on an Agilent Bio-analyser High Sensitivity DNA chip (Agilent technologies, #5067–4626).
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bioRxiv - Neuroscience 2021Quote: ... N2081D and G2385R mutant GFP-tagged LRRK2 constructs were generated by site-directed mutagenesis (QuikChange, Stratagene), and identity of all constructs verified by sequencing of the entire coding region ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in the secondary antibody-horseradish peroxidase (HRP) complex from the REAL EnVision detection kit (Dako #K5007) for 1h at RT ...