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Citations for Agilent :
101 - 150 of 190 citations for PEG NH2 modified upconverting red light since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Size Exclusion Chromatography in line with Multi Angle Laser Light Scattering (SEC-MALLS) experiments for absolute mass determination were conducted at 25°C with a BioSEC-3 column (Agilent) equilibrated in 20 mM Na-phosphate pH 7.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... 3-4 cm away from the light source under a long-wave UV bulb (350 nm) in a UV Stratalinker 2400 (Stratagene) for seven minutes with mixing breaks every two minutes ...
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bioRxiv - Genetics 2023Quote: ... ND-1000 UV-visible light spectrophotometer (Nanodrop Technologies) and Bioanalyzer 2100 with the RNA 6000 Nano Lab Chip kit (Agilent) was used to assess the concentration and integrity of RNA.
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bioRxiv - Microbiology 2021Quote: ... was obtained by using a modified Quick-Change II Site-Directed Mutagenesis kit (Agilent). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... coated Seahorse XF96 plates in 200 μL XF Assay Medium (Modified DMEM, Seahorse Bioscience), respectively ...
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bioRxiv - Microbiology 2021Quote: ... Activity was monitored spectrophotometrically using a modified Cary 60 UV/Vis Spectrophotometer (Agilent Technologies), following the oxidation of NADH or NADPH at 340 nm in the presence of ubiquinone-1 (UQ-1 ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were unmasked by heating in modified citrate buffer S1700 (Dako, Glostrup, Denmark). Then ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... coated Seahorse XF96 plates in 200 μL XF Assay Medium (Modified DMEM, Seahorse Bioscience). For OCR measurements ...
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bioRxiv - Biophysics 2023Quote: Human FKBP12.6 cDNA was modified by site directed mutagenesis (QuikChange Lightning kit; Agilent Technologies) to introduce one of eight single cysteine mutants (G1C ...
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bioRxiv - Cell Biology 2024Quote: ... GFP-tagged COPI cDNA constructs were modified by QuikChange II site-directed mutagenesis (Agilent) in conjunction with SNP-specific primers (Supplementary Table 2 ...
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bioRxiv - Microbiology 2021Quote: ... A bright red chromogen labelling was produced with 3-amino-9-ethylcarbazole substrate (AEC, DAKO). Sections were counterstained with Mayer’s haematoxylin ...
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bioRxiv - Immunology 2024Quote: ... Red blood cells were then lysed with lysis buffer (EasyLyse, Agilent Technologies, Santa Clara, USA) and cells were washed twice in staining buffer ...
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bioRxiv - Biophysics 2021Quote: SEC-MALS experiments were performed by using a miniDAWN TREOS static light scattering detector (Wyatt Technology Corp.) combined with an HPLC system (1260 Infinity LC, Agilent Technologies). The volume 100 μl of 200-500 μM protein samples in PBS buffer (pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: Oligomeric states for the designs in solution were studied by SEC-MALS with miniDAWN TREOS static light scattering detector (Wyatt Technology Corp.) combined with a HPLC system (1260 Infinity LC, Agilent Technologies) with a Superdex 75 increase 10/300 GL column (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: ... In vivo psoralen cross-linking of the DNA was achieved by a repetitive exposure of living cells to 4,5’,8-trimethylpsoralen (10 μg/ml final concentration) followed by irradiation pulses with UV 365-nm monochromatic light (UV Stratalinker 1800; Agilent Technologies). The cells were then lysed with cell lysis buffer (buffer C1 ...
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bioRxiv - Neuroscience 2021Quote: ... For ChR2-mediated optogenetic stimulation, blue light (473 nm, 6 Hz or 20 Hz) was produced using an arbitrary waveform generator (Agilent, 33220A) and a diode-pumped solid-state laser (Laserglow ...
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bioRxiv - Biochemistry 2022Quote: Size exclusion chromatography combined with multiangle light-scattering (SEC-MALS) was carried out on an HPLC system (Agilent LC1100, Agilent Technologies) coupled to an Optilab rEX refractometer and a miniDAWN three-angle light-scattering detector (Wyatt Technology) ...
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bioRxiv - Biochemistry 2022Quote: Size exclusion chromatography combined with multiangle light-scattering (SEC-MALS) was carried out on an HPLC system (Agilent LC1100, Agilent Technologies) coupled to an Optilab rEX refractometer and a miniDAWN three-angle light-scattering detector (Wyatt Technology) ...
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bioRxiv - Cell Biology 2022Quote: ... The sulfo-SANPAH solution was placed on gels and activated using 1 Joule of 254 nm UV light (Stratagene UV crosslinker). Afterwards ...
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bioRxiv - Genomics 2023Quote: Cells were allowed to grow to confluency and UV crosslinked using 254nm UV light in Stratalinker 2400 on ice (Stratagene, USA). UV treated cells were lysed to get different cellular fractions (Cytoplasmic ...
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bioRxiv - Biophysics 2023Quote: ... The protein yield is estimated by light absorption at 280 nm using the Biotek Synergy H4 plate reader equipped with the Take3 microvolume plate (Agilent, USA).
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bioRxiv - Cancer Biology 2023Quote: ... DNA was cross-linked by exposing the living cells twice to 4,5′,8-trimethylpsoralen at a final concentration of 10 μg/mL followed by 3’ irradiation pulses with UV 365-nm monochromatic light (UV Stratalinker 1800, Agilent Technologies). The cells were then washed repeatedly with cold PBS and lysed with a cell lysis buffer (1.28M sucrose ...
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bioRxiv - Plant Biology 2024Quote: ... Vials were placed on ice and illuminated for 7 min in “time mode” using a UV-crosslinker with a 254 nm light source (Stratalinker Model 1800, Stratagene, USA). Each sample had a corresponding photolysis negative control ...
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bioRxiv - Immunology 2021Quote: ... additions and point mutations were generated using a modified QuikChange site-directed mutagenesis protocol (Agilent). RBD-ferritin and S1-ferritin constructs were synthesized by GenScript ...
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bioRxiv - Synthetic Biology 2021Quote: Amplex Red experiments (described further below) were monitored on a Cary 60 UV-vis (Agilent Technologies) or a SpectraMAX 340PC plate reader (Molecular Devices ...
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bioRxiv - Immunology 2024Quote: ... Red fluorescent and brightfield images were captured with the Cytation1 plate reader (Agilent, Santa Clara, CA) using a 4x objective to capture 2 x 2 image montages in 1-hour intervals for 24 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Assay medium consisted of XF Base Medium Minimal DMEM with phenol red (#103334-100, Agilent Technologies), 1 mM pyruvate ...
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bioRxiv - Microbiology 2020Quote: ... DNA was cross-linked to the membrane by exposure to 120,000 µjoles of UV light using a UV Stratalinker 2400 (Stratagene, San Diego, California).
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bioRxiv - Molecular Biology 2023Quote: ... seedlings were exposed on the plates to 8 kJ/m2 UV-C light in a Stratalinker 2400 (Stratagene, La Jolla, California, US) and transferred back into growth chamber for 5 h ...
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bioRxiv - Immunology 2021Quote: ... RBD with VOC point mutations were generated using a modified QuikChange site-directed mutagenesis protocol (Agilent). The constructs resulting from site-directed mutagenesis were amplified and isolated from E ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed with XF base media (HCO3- free modified DMEM, Agilent, Santa Clara, California, USA) supplemented with 1 mM sodium pyruvate (Gibco) ...
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bioRxiv - Physiology 2024Quote: ... Assay medium consisted of Sea Horse XF base medium without phenol red (Agilent Technologies, Cat#103335-100) supplemented with glucose (25 mM) ...
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bioRxiv - Immunology 2022Quote: ... Influenza A virus inactivation was performed by delivering 2400 μJ/cm2 of UV light (254 nm) using a Strata linker 1800 (Stratagene, La Jolla, CA.). Virus inactivation was verified by plaque assay ...
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bioRxiv - Immunology 2024Quote: ... Replication-defective viruses were prepared by delivering 2400 µJ/cm2 of UV light (254 nm) using a Strata linker 1800 (Stratagene, La Jolla, CA). Inactivation of Cal/09 (Cal09-UV ...
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bioRxiv - Neuroscience 2022Quote: ... 200.000 HoxB8-immortalized granulocytes were cultured in XF Base Medium Minimal Dulbecco’s Modified Eagle’s Medium (Agilent Technologies) containing 10 mM glucose ...
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bioRxiv - Neuroscience 2022Quote: ... The pT7-7 plasmid was also modified using site directed mutagenesis (#200523, QuikChange II, Agilent, Waldbronn, Germany) to incorporate a cysteine residue at position 141 to permit dye-labelling of the aSyn protein ...
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bioRxiv - Molecular Biology 2023Quote: ... A GMP grade chemically modified single guide RNA (sgRNA) targeting the HBB locus was purchased from Agilent with modifications for 2ʹ-O-methyl-3ʹ-phosphorothioate at the three terminal nucleotides of the 5ʹ and 3ʹ ends with the sequence 5ʹ-CTTGCCCCACAGGGCAGTAA-3ʹ ...
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bioRxiv - Plant Biology 2020Quote: ... The filtrate was injected into Spherisorb 5μm ODS column (250 mm x 4.6 mm) for HPLC (model 1260) coupled to 1200 series evaporative light scattering detector (Agilent Technologies, Santa Clara, CA, USA). This analysis was conducted twice independently with 2 to 3 technical replications each ...
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bioRxiv - Genomics 2023Quote: UV inactivation of virus was performed by delivering 1800 MJ of UV-C light (254 nm) to 250 uL of undiluted viral stock in a 24-well plate using a Stratalinker 1800 (Stratagene California, La Jolla, CA) inside a BSC in the BSL3 ...
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bioRxiv - Molecular Biology 2024Quote: ... and Seahorse XF DMEM medium (without Phenol Red/pH 7.4/with HEPES/500 mL, Agilent, Cat# 103575-100) to quantify Oxygen Consumption Rate (OCR ...
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bioRxiv - Molecular Biology 2024Quote: ... and Seahorse XF DMEM medium (without Phenol Red/pH 7.4/with HEPES/500 mL, Agilent, Cat# 103575-100) to quantify oxygen consumption rate (OCR ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were incubated in 500 µL Seahorse XF Base Medium Minimal DMEM without Phenol Red (Agilent, 103335-100) 1 h at 37 °C in CO2 free-atmosphere ...
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bioRxiv - Neuroscience 2023Quote: ... the neuronal maintenance medium was exchanged with XF DMEM Base Medium (pH 7.4) with no phenol red (Agilent) supplemented with 5 mM glucose and 1 mM pyruvate ...
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bioRxiv - Cell Biology 2023Quote: ... the cell maintenance medium was exchanged with XF DMEM Base Medium (pH 7.4) with no phenol red (Agilent) supplemented with 1mM sodium pyruvate (Corning Cellgro) ...
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bioRxiv - Cell Biology 2023Quote: ... the neuronal maintenance medium was exchanged with XF DMEM Base Medium (pH 7.4) with no phenol red (Agilent) supplemented with 5 mM glucose and 1 mM pyruvate ...
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bioRxiv - Genetics 2021Quote: ... cloned into an expression plasmid driven by CMV promoter and modified using QuikChange Site-Directed Mutagenesis Kit (Agilent). For each affinity purification ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by an antigen retrieval step in a modified citrate buffer (Dako Target Retrieval Solution, pH 6.1, Agilent). Sections were then incubated in Sudan Black (0.3% in 70% ethanol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The growth medium was replaced with XF test medium (Eagle’s modified Dulbecco’s medium, 0 mM glucose, pH=7.4; Agilent Seahorse) supplemented with L-glutamine (1 mM) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Growth medium was replaced with XF test medium (Eagle’s modified Dulbecco’s medium, 0 mM glucose, pH=7.4; Agilent Seahorse) supplemented with 1 mM pyruvate ...
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bioRxiv - Cancer Biology 2023Quote: ... the culture medium in the assay plate was replaced with XF assay medium modified DMEM (102365-100, Agilent) supplemented with 1 mM Pyruvate ...