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Citations for Agilent :
101 - 150 of 160 citations for Hydroxy PEG4 t butly ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibody in TBS-T (1:3000, Dako, Glostrup, Denmark) for 1 hour at RT ...
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bioRxiv - Physiology 2024Quote: ... we performed the bioenergetic analysis using the Seahorse T-cell Mitochondrial profiling kit (part. no.: 103771-100, Agilent) with a final concentration of 4mM Oligomycin ...
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bioRxiv - Neuroscience 2024Quote: ... and five control dogs were evaluated with antibodies (Abs) against CD3 (T cells) (M7254, Ms mAb; Agilent Dako), CD79b (B cells ...
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bioRxiv - Neuroscience 2024Quote: ... and five control dogs were evaluated with antibodies (Abs) against CD3 (T cells) (M7254, Ms mAb; Agilent Dako), CD79b (B cells ...
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bioRxiv - Neuroscience 2024Quote: ... washed with TBS-T and next incubated with secondary HRP-conjugated anti-rabbit IgG (1:2000, DAKO, P0448) and developed using the ECL detection kit (PerkinElmer ...
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bioRxiv - Physiology 2024Quote: ... washed with TBS-T and next incubated with secondary HRP-conjugated anti-rabbit IgG (1:2,000, DAKO, P0448) for 1 hour at room temperature and developed using the ECL detection kit (Perkin Elmer ...
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bioRxiv - Physiology 2024Quote: MRI experiments were performed on a 9.4-T/31-cm small animal magnet (Agilent, Palo Alto, CA, USA) interfaced to a Bruker Avance NEO console (Bruker BioSpin Corp ...
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... The membranes were washed with TBS-T and incubated with corresponding HRP conjugated secondary antibody (Agilent Dako, Stockport, UK) for 1h at room temperature ...
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... The membranes were washed with TBS-T and incubated with corresponding HRP conjugated secondary antibody (Agilent Dako, Stockport, UK) for 1h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and 10% Methanol for 10 min and incubated overnight with the primary antibody (Table 2) in PBS/T 0.1 with 10 % normal swine/ goat or rabbit serum (Dako). Second ...
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bioRxiv - Microbiology 2021Quote: ... and quantification of their tertiary butyldimethylsisyl (t-BDMS) derivatives using capillary GC apparatus (Agilent 6890; Agilent Technologies, Santa Clara). Two technical replicates of an external standard (acetic acid ...
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bioRxiv - Pathology 2021Quote: ... Slides were again washed in TBS-T two times followed by incubation with anti-rabbit EnVision detection kit (DAKO) for 1 hour in room temperature ...
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bioRxiv - Immunology 2024Quote: T cell oxygen consumption rate (OCR) was measured using a Seahorse XFe96 Extracellular Flux Analyzed following established protocols (Agilent). Briefly ...
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bioRxiv - Immunology 2024Quote: ... 400,000 T cells/well were plated onto Poly-D-lysine coated cell culture plate in RPMI XF media (Agilent) supplemented with 10mM glucose (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed 2 x 5 min in PBS-T and incubated with streptavidin–horseradish peroxidase complex (DakoCytomation). Following 2 x 5 min washes in PBS-T ...
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bioRxiv - Cancer Biology 2024Quote: ... Blood T cell fractions were estimated using TcellExTRECT using exons from the corresponding capture kit (Agilent custom V2 Exome) with a median coverage threshold set to five.
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bioRxiv - Biochemistry 2021Quote: All NMR-spectra were acquired using a triple resonance room temperature probe at a static magnetic field of 14.1 T (Agilent Technologies) at 25 °C in 5 % D2O ...
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bioRxiv - Biochemistry 2020Quote: The supernatants of the quenched in vitro OMT reactions and fermentation samples were analyzed by reversed-phase HPLC (instrument: Agilent 1100; autosampler: HiP sampler G1367A, T=4°C, 10 μL injection; column: Agilent Zorbax Eclipse XDB-C18 80Å ...
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bioRxiv - Microbiology 2021Quote: ... The SRE* reporter was generated by introducing an A to T point mutation at position 74 of the SRE using the Quickchange site directed mutagenesis protocol (Agilent) using the primers described in Table S1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Animals treated with CAR T cells had tumors excised and were also stained with H&E and human CD3 (A0452, Dako), in addition to GPC2 ...
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bioRxiv - Immunology 2021Quote: ... 3×105 CD8+ T cells were plated onto poly-D-lysine coated wells and assayed in XF RPMI medium (Agilent) pH 7.4 supplemented with 10 mM glucose and 2 mM glutamine ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were washed three times with TBS-T and incubated for 1 hour with HRP-conjugated secondary antibodies (Dako). Proteins were detected by enhanced chemiluminescence reagents (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... the final nucleotide of the TATA box of pCMV-Gluc2 was changed to a T by using site-directed mutagenesis (QuikChange lightning; Agilent) to facilitate in vitro transcription (54) ...
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bioRxiv - Genetics 2020Quote: ... The A>T variant was introduced to PIK3C2B-pNL construct by site-directed mutagenesis using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) to generate PIK3C2B-A>T-pNL construct ...
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bioRxiv - Biochemistry 2022Quote: Full-length and loopless Ndc80 complexes were analysed by SEC-MALS on a Dawn Heleos II System with an Optilab T-rEX RI detector (Wyatt) and a 1260 Inifinity II LC system (Agilent). The Superose 6 increase 10/300 column (GE Healthcare ...
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bioRxiv - Systems Biology 2023Quote: ... 50μL of 200mM ammonium formate pH10 were added to samples and “Fractionation V2.0” was ran on AssayMap BRAVO (Agilent T). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed three times for 20 min in TBS-T before incubation with horseradish peroxidase (HRP)-labeled secondary antibody in TBS-T at RT for 1 hour: Goat anti-Rabbit HRP (1:10,000, Agilent P0448), Rabbit anti-Goat HRP (1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... and 1-2×105 T cells per well were attached in 5-8 replicates in Seahorse XF RPMI medium (Agilent) supplemented with 2 mM L-glutamine (Gibco) ...
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bioRxiv - Biochemistry 2023Quote: ... Shimadzu LC-10AT; autosampler: HiP sampler G1367A, T = 4°C, 10 µL injection; flow rate: 1 mL/min; column: Agilent Zorbax Eclipse XDB-C18 80Å ...
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bioRxiv - Cancer Biology 2023Quote: ... tissue was permeabolized with 0,5% Triton X-100 in PBS (PBS-T) and non-specific binding was blocked by incubating the tissue section with blocking reagent (DAKO). Antibody staining was performed in PBS-T with 1% blocking reagent (DAKO ...
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bioRxiv - Cancer Biology 2024Quote: ... The tissue was washed twice in TBS-T prior incubation with secondary antibody (EnVision anti-mouse-HRP/DAB+ system, Agilent), 3,3′-Diaminobenzidine (DAB ...
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bioRxiv - Microbiology 2024Quote: Solid-state 13C CPMAS NMR (Schaefer & Stejskal, 1976) experiments were performed using an 89-mm bore 11.7 T magnet (Agilent Technologies) with an Agilent triple resonance BioMAS probe and DD2 console (Agilent Technologies) ...
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bioRxiv - Biophysics 2024Quote: ... cysteines for labeling were introduced into positions A153C and A213C flanking the troponin-T linker region using QuikChange mutagenesis (Agilent), which we will refer to as TnT153,213 ...
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bioRxiv - Neuroscience 2024Quote: ... blots were washed 3 times during 10 min with PBS-T and incubated with horseradish peroxidase-conjugated secondary antibody (Dako) for 1 h and washed again 3 times ...
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bioRxiv - Microbiology 2024Quote: Seahorse analyses of the energy metabolism in activated CD4 T cells were performed using the ATP rate assay (Seahorse Biosciences, Agilent) following manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... The high-SBP rs9833313-T allele was introduced by site-directed mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and confirmed by sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was also detected as it eluted from the column with a differential refractometer (Optilab T-rEX, Wyatt Technology) and a UV detector at 280 nm (Agilent 1260 UV, Agilent Technology). Molar masses of peaks in the elution profile were calculated from the light scattering and protein concentration ...
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bioRxiv - Immunology 2021Quote: ... membranes were incubated for 45 minutes in 1% dried skim milk in PBS-T with rabbit anti-human C1q (1:300, Dako, A0136) and HRP-conjugated goat anti-rabbit IgG (1:10.000 ...
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bioRxiv - Immunology 2023Quote: ... brucei parasites using a polyclonal rabbit antibody raised against T. brucei luminal binding protein 1 (BiP) (J. Bangs, SUNY, USA) using a Dako Autostainer Link 48 (Dako, Denmark) and were subsequently counterstained with Gill’s Haematoxylin ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed a further three times with TBS-T for 5 minutes and stained with DAKO EnVision-HRP rabbit/mouse (Agilent; #K5007) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed a further 3 times in TBS-T for 5 minutes and developed with DAB diluted at a 1:50 ratio in DAB-chromagen (Agilent; #K3468) until appearance of brown staining ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were washed in TBS-t and subsequently incubated with goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody (0.5 µg/ml; Dako, VIC, Australia) or rabbit anti-Goat IgG horseradish peroxidase-conjugated secondary antibody (0.125 µg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... The antibody-vWF complexes were washed with PBS-T (0.1%) three times followed by incubation with an anti-vWF antibody (Dako rabbit polyclonal) conjugated with biotin for 60 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: All MRI and 1H MRS data were acquired in a 9.4 T Varian Direct Drive system (Agilent Technologies, Santa Clara, CA, USA). A 14 mm diameter receive-only surface RF coil (Doty Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... MD and mutated at AKAP12’s activation-responsive sites (S/T to A mutations) using the QuikChange II® site-directed mutagenesis Kit (Stratagene, CA) as we described previously (54) ...
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bioRxiv - Cancer Biology 2022Quote: ... The membranes were washed thrice for 5 min in TBS-T and incubated with Horseradish peroxidase secondary antibodies (DAKO, Santa Clara, USA) for 1 hr at RT ...
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bioRxiv - Neuroscience 2023Quote: MRI measurements were done with a 9.4-T magnet (bore diameter 31 cm) interfaced with a DirectDRIVE console (Agilent Technologies, Inc., USA) and with a 21-cm-inner-diameter gradient coil set ...
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bioRxiv - Biophysics 2024Quote: 13C cross-polarization magic-angle spinning (CPMAS) solid-state NMR was performed at ambient temperature in an 89 mm bore 11.7 T magnet (Agilent Technologies, Danbury, CT) using an HCN Agilent probe with a DD2 console (Agilent Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... A wash step with TBS-T was performed between all steps using a BioTek 405TS auto-washer (Agilent technologies, Santa Clara, CA). A two-way ANOVA analysis combined with the Tukey test was used for statistical analysis.
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bioRxiv - Biophysics 2021Quote: ... All mice were anaesthetized (1.5-1.8% isoflurane in 1.5 l/min air with balance in oxygen) and scanned on a 9.4 T Agilent system (Agilent Technologies, Santa Clara, CA, USA) using a 33-mm-diameter transmit/receive coil (Rapid Biomedical GmbH ...