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Citations for Agilent :
1401 - 1450 of 4398 citations for 5 Pyrimidinecarbonitrile 1 2R 2 3 dihydroxypropyl 1 2 3 4 tetrahydro 3 methyl 2 4 dioxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: Point substitutions within RBD in SARS-CoV-2 spike gene were introduced by site-directed mutagenesis using the QuikChange II kit (Agilent Technologies Inc.) following the manufacturer’s protocol and by overlapping PCR strategy as described previously (33) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A 2 µl aliquot of processed gDNA was taken to assess shearing using an Agilent High Sensitivity D1000 Kit (Agilent, Cat. #G2991AA). Once shearing was assessed ...
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bioRxiv - Microbiology 2023Quote: ... 200 mm by 2 mm fitted with a precolumn 10 mm by 2 mm (Dr. Maisch GmbH, Ammerbuch, Germany) coupled to an Agilent LC/MSD Ultra Trap System XCT 6330 (Agilent, Waldbronn, Germany). For analysis were used ...
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bioRxiv - Plant Biology 2020Quote: ... Hybridizations were done using a custom 4×44 k oligoarray (Agilent Technologies) that was previously described15,25 ...
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bioRxiv - Cancer Biology 2020Quote: ... 4×180K or 8×60K SurePrint G3 human CGH microarray (Agilent Technologies) according to manufacturer’s instructions (CGH enzymatic protocol v6.2 ...
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bioRxiv - Immunology 2020Quote: ... FLAG-tagged YTHDF1 was cloned into pCMV-Tag 4 vector (Agilent, #211174); Myc-tagged DDX60 was cloned into pRK-5 vector (BD PharMingen ...
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bioRxiv - Genomics 2021Quote: ... diluted to 4 nM and QC’d on the Bioanalyzer (Agilent Technologies, USA). The final pool was sequenced on Illumina MiSeq platform 2×300 bp using the MiSeq Reagent Kit V3 (600 cycles PE ...
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bioRxiv - Physiology 2023Quote: ... at 4 °C overnight and mounted with fluorescence mounting medium (Agilent, USA).
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bioRxiv - Developmental Biology 2023Quote: ... The signal was detected with BioTek Synergy 4 Microplate reader (Agilent Technologies), and the results were analyzed using a 4-parameter logistic regression algorithm (http://www.elisaanalysis.com/app) ...
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bioRxiv - Neuroscience 2024Quote: ... Wall air was passed through a hydrocarbon filter (Agilent Technologies, HT200-4) and split into a 100 mL/min odor stream and 900 mL/min carrier stream using analog flowmeters (Cole-Parmer ...
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bioRxiv - Physiology 2022Quote: ... for 30 minutes and stained with insulin antibody (dilution 1:5, IR002, Dako) for 1 hour at room temperature and washed 3 times with 1X PBS ...
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bioRxiv - Immunology 2022Quote: ... Unspecific binding sites were blocked with Normal Goat Serum (1:5 dilution, DAKO) in antibody diluent (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... As described in manufacturer’s protocol (Agilent SureSelectXT Mouse Methyl-Seq Kit), bisulfite converted libraries were PCR-amplified for 8 cycles with supplied universal primers and purified using AMPure XP beads ...
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bioRxiv - Cell Biology 2021Quote: ... and 299.294 m/z (Methyl Stearate; Agilent article number G1982-85003) were used to recalibrate spectra during the acquisition.
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bioRxiv - Microbiology 2021Quote: ... and analysed as fatty acid methyl esters by gas chromatography (Agilent 6890N ...
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bioRxiv - Cancer Biology 2021Quote: ... the membranes were washed 3X with TBST followed by incubation with HRP-conjugated secondary antibodies (Rabbit Cytiva/Amersham NA934; Mouse Agilent/Dako P026002-2) for one hour at room temperature ...
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bioRxiv - Genetics 2020Quote: ... and 0.5 mL was pipetted into individual 2 mL glass vials fitted with 9mm PTFE lined caps (Agilent Crosslab, Santa Clara, CA, USA). The hexane was evaporated under a nitrogen gas flow ...
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bioRxiv - Cell Biology 2020Quote: ... RNA quality of all samples (n=7 time points and n=3 experiments from independent passages) was evaluated by both Nanodrop for (A260/280 >2) and Bioanalyzer 2100 High Sensitivity RNA Analysis chips (Agilent, Cat. 5067-1513) which displayed intact RNA integrity (RIN >9) ...
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bioRxiv - Developmental Biology 2021Quote: Immunofluorescence microscopy was performed on de-paraffinized tissue sections that received heat-induced antigen retrieval using Target Retrieval Solution (Agilent Dako, S169984-2). After washing and permeabilization in TBS-T universal buffer (0.2% Triton X-100 in tris-buffered saline) ...
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bioRxiv - Genomics 2020Quote: ... a plasmid encoding the replicases of AAV serotype 2 and the capsid of AAV serotype 9) and pHelper (Agilent Technologies, lab reference pZMB0088) which provides AAV adenoviral helper functions ...
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bioRxiv - Biochemistry 2019Quote: ... in 200μl of dichloromethane containing 200 ng of 2-tetradecyl acetate (internal standard) in 2ml glass vials with polytetrafluoroethylene-coated caps (Agilent, Santa Clara, California). After one hour ...
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bioRxiv - Biochemistry 2019Quote: ... solvent A) and methanol:isopropanol (8:2 v/v, solvent B) was generated by an Infinity II 1290 UPLC (Agilent, Santa Clara, CA, USA) and with a constant flow rate of 600 μL min−1 (0 min ...
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bioRxiv - Genomics 2020Quote: ... Chromium Genome Reagents Kit Version 2 User Guide) and size and concentration determined using an Agilent 2100 Bioanalyzer DNA 1000 chip (Agilent Technologies, CA, USA). Libraries were then sequenced on an Illumina NovaSeq 6000 System following the manufacturer’s protocols (Illumina ...
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bioRxiv - Bioengineering 2021Quote: ... samples were subjected to a treatment with 0.3% Sudan black solution for 10 min prior to blocking for 2 h (Dako Blocking Solution X0909; Agilent Technologies, Santa Clara, CA). Immunofluorescence staining was then performed using antibodies to detect CD31 (dilution 1:250 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Amino acid substitutions or deletions were introduced into the pSARS-CoV-2-Strunc expression vector by site-directed mutagenesis (Stratagene, La Jolla, CA) using mutagenic oligonucleotides as follow:
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bioRxiv - Cell Biology 2021Quote: Both PCRs were performed in 100 µl with the 2 µl of the Herculase II Fusion DNA Polymerase from Agilent (ref. no. 600675) according the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... and 0.5 mL was pipetted into individual 2 mL glass vials fitted with 9mm PTFE lined caps (Agilent Crosslab, Santa Clara, CA, USA). The hexane was evaporated under a nitrogen gas flow ...
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bioRxiv - Cancer Biology 2021Quote: ... before low pH antigen retrieval and staining with CD8a and Ki67 (Supplementary Table 7) antibodies and secondary antibody (EnVision+ HRP-rabbit, Dako, catalog #K400311-2) using the EnVision DuoFlex system (Dako) ...
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bioRxiv - Neuroscience 2020Quote: ... and heat-induced antigen retrieval was performed using 10mM sodium citrate solution (pH 6.0, pSer129) or citrate Target Retrieval Solution (Dako #S169984-2, pH 6.1) with 0.05% Tween-20 (conformation-specific ...
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bioRxiv - Physiology 2022Quote: ... All assays were run in duplicate under control conditions or in the presence of 5 mM DTT in a 96-well plate and changes in absorbance were tracked using a microplate reader (Synergy 2, BioTek/Agilent, Santa Clara, CA). This concentration was selected based on similar experiments in a previous study [15] ...
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bioRxiv - Immunology 2022Quote: FFPE tissue sections were deparaffinized and antigen retrieval was undertaken in 0.1 M citric acid buffer solution (Dako, target retrieval solution, S169984-2) using an electric pressure cooker (Russell Hobbs RHNHP401) ...
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bioRxiv - Biochemistry 2021Quote: ... and 12 were used as primary detection against T22 capture antibody) was an anti-tau polyclonal rabbit antibody (A002401-2) purchased from Agilent (Santa Clara, CA). A goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen □ retrieval was performed by incubating the tissue for 2 h at 70 °C in Target Retrieval Solution (Dako, Santa Clara, CA, USA) using a 1:10 dilution in demineralized water ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 µg of the plasmid carrying the relevant recombinant provirus was co-transfected with 2 µg of plasmid pMD2.G (a kind gift from D. Trono) and 2 µg of plasmid PVPackgagpol (#217566 from Stratagene, La Jolla, CA) or plasmid pCMV-1′R8.74 (a gift from D ...
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bioRxiv - Cancer Biology 2023Quote: ... using the clone DO-7 monoclonal antibody (GA61661-2, Dako Omnis, ready-to-use, incubation 20 min, Agilent Technologies, Santa Clara, CA, USA). Antibody detection was performed using EnVision FLEX/HRP (GV80011-2 ...
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bioRxiv - Genetics 2023Quote: ... Double-stranded cDNA was synthesised from 2 μg of total RNA using AccuScript High Fidelity 1st Strand cDNA Synthesis Kit (Stratagene, La Jolla, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and Cal27 were seeded at a density of 2×104 cells per well in a Seahorse XFe24 Cell Culture Microplate (102340-100; Agilent, Santa Clara, CA). Cells were incubated overnight for adherence in a culture media composed of DMEM and 10% FBS at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... The antigen retrieval step was repeated followed by second round staining for pan-CK using anti-pan-cytokeratin antibody (AE1/AE3 mouse monoclonal, Dako-Cat# M351501-2) followed by HRP-conjugated anti-mouse secondary antibody polymer (EnVision+ ...
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bioRxiv - Neuroscience 2023Quote: The AAV plasmid of human synapsin I promoter (hSyn)-tetracycline-controlled transactivator 2 (tTA2) was constructed by subcloning the DNA fragments containing hSyn and tTA2 into pAAV-MCS (Agilent Technologies, CA, USA). The AAV vector was produced as described previously (Ebina et al. ...
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bioRxiv - Microbiology 2023Quote: ... Plate was shaken continuously at 30°C and OD600 was read every 20 minutes in a BioTek Epoch 2 Microplate Spectrophotometer (Agilent, Santa Clara, CA). MMC (0.5 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... The AAV plasmid of human synapsin I promoter (hSyn)-tetracycline-controlled transactivator 2 (tTA2) was constructed by subcloning the DNA fragments containing hSyn and tTA2 into pAAV-MCS (Agilent Technologies, CA, USA). The AAV vector was produced as described previously15,35,55 ...
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bioRxiv - Microbiology 2023Quote: ... followed by sample measurements, all performed in duplicates using the OT-2 robot (Opentrons, Long Island City, NY) and BioTek Synergy (Agilent Technologies, Waldbronn, Germany). The acquired data were evaluated using the Shiny application based on the determined standard curves for accurate quantification [62].
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bioRxiv - Microbiology 2024Quote: ... The growth was monitored by measuring the optical density at 600 nm (OD600) in an Epoch 2 plate reader (Agilent, Santa Clara, CA). The growth rates were calculated using the R package growthcurver (32).
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bioRxiv - Immunology 2022Quote: ... pooled at 4 nM and quality-assessed on a 2100 Bioanalyzer (Agilent Technologies). Sequencing was performed on an Illumina MiSeq (paired-end ...
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bioRxiv - Cancer Biology 2020Quote: ... SureFISH chr4 CEP 613kb green for the centromere of chromosome 4 (#G101066G, Dako), FISH RAMP1 red for the RAMP1 gene (#G110996X-8 ...
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bioRxiv - Immunology 2021Quote: ... Cy-dye labelling and hybridization to Agilent High Definition 4×44k array (Agilent technologies ...
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bioRxiv - Physiology 2022Quote: ... We used a Mouse Gene Expression 4 × 44K Microarray chip (G4846A, Agilent Technologies), which can examine 23,215 genes as described [30] ...
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bioRxiv - Cancer Biology 2023Quote: ... with small RNA ladder (4-150nt) were used (cat no. 5067-1550, Agilent), all accessory reagents were also from Agilent (cat no ...
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bioRxiv - Immunology 2023Quote: ... mCTLA-4 cDNA was sub-cloned into the pCMV-Tag4A vector (Agilent Technologies) and an exon3 deletion construct was made by the KOD Plus Mutagenesis Kit (TOYOBO) ...
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bioRxiv - Immunology 2023Quote: ... The tissue was then incubated overnight at 4°C with anti-CD4 (Dako) in a 1:10 dilution ...