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Citations for Agilent :
1401 - 1450 of 1708 citations for 2' Chloro 3 2 3 dimethylphenyl 4' fluoropropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plates were incubated at room temperature for 30 minutes and FRET signals (665 and 620 nm) were read using Biotek Synergy Neo 2 plate reader (Agilent, Santa Clara, CA). HTRF signal was calculated as the ratio of signal from the 665 nm (acceptor ...
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bioRxiv - Bioengineering 2024Quote: ... centrifuged at 1000 x g for 5 minutes and resuspended in 2 mL of assay media (Seahorse XF base medium without phenol red (Agilent, Cat. # 103334-100), 1 mM pyruvate (Acros Organics ...
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bioRxiv - Cancer Biology 2024Quote: ... 2×105 neutrophils were resuspended in 50 µL neutrophil mitochondria assay media containing Seahorse Agilent RPMI media (Agilent Technologies, Cat. #103576-100, US), 1 mM pyruvate ...
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bioRxiv - Bioengineering 2024Quote: ... samples were treated with 0.3% Sudan black solution for 10 min prior to blocking for 2 h (Dako Blocking Solution X0909; Agilent Technologies, Santa Clara, CA). Immunohistochemical staining was then performed using antibodies to detect laminin (dilution 1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... β1-4 galactosidase (Agilent Technologies), or double digestion with Sialidase A and β-galactosidase ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-LMP1 (CS1-4, Dako), anti-LMP2A (MCA2467 ...
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bioRxiv - Immunology 2024Quote: ... β(1-4)-Galactosidase (Prozyme) (designated as “G”) ...
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bioRxiv - Microbiology 2021Quote: ... The R682S furin cleavage mutation was introduced into the SARS-CoV-2 S expression plasmid by QuikChange site-directed mutagenesis (Agilent Technologies, Santa Clara, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: All AAV (serotype 2) vectors were produced by triple transfection of HEK293T cells using a rep/cap plasmid and pHelper (Stratagene, La Jolla CA, USA), and purified by column affinity chromatography as previously described (104) ...
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bioRxiv - Neuroscience 2020Quote: All AAV (serotype 2) vectors were produced by triple transfection of 293 cells using a rep/cap plasmid and pHelper (Stratagene, La Jolla CA, USA), and purified by column affinity chromatography as previously described38 ...
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bioRxiv - Cancer Biology 2021Quote: ... qRT-PCR was performed as previously described (Sodi et al., 2015) using Brilliant II qRT-PCR Master Mix 2 Kit (Stratagene, San Diego, CA, USA) using Applied Biosystems 7500 machine. ...
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bioRxiv - Synthetic Biology 2020Quote: ... The hybridisation mix was prepared by mixing the specified amounts of RNA with 10× Gene Expression Blocking Agent (1× final conc.) and 2× Hi-RPM Hybridization Buffer (1× final conc.) (Agilent, Cat No. 5188-5242) in a final volume of 42 μl ...
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bioRxiv - Immunology 2021Quote: ... The size of the amplicon was confirmed by analyzing 2 μl of PCR products using the Agilent D5000 ScreenTape System (Agilent D5000 ScreenTape, 5067-5588, Agilent D5000, Reagents 5067-5589). Products were quantified by analyzing 2 μl with the Quant-iT dsDNA High-Sensitivity Assay Kit (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: Fraction HMO-2 was digested by treatment of 10 µg dried sample with β-galactosidase from bovine testes (10 mU, Prozyme, Invitrogen, Karlsruhe, Germany) in 20 µl of 0.1 M citrate/phosphate buffer ...
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bioRxiv - Synthetic Biology 2022Quote: ... Growth experiments were conducted (either at 100% air or 90% air / 10% CO2) in a BioTek Epoch 2 plate reader (Agilent, Santa Clara, CA, USA) at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated for 20 minutes in a microwave oven in DAKO Target Retrieval pH6 (S203130-2, DAKO, Agilent Technologies, Santa Clara, CA, USA). Then ...
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bioRxiv - Microbiology 2023Quote: ... One microliter of each methoximated and trimethylsilylated sample was injected by 2:1 split injection by an Agilent Technologies 7693 autosampler (Agilent Technologies, Santa Clara; California) into an Agilent 7890A gas chromatograph equipped with a fused silica capillary column (0.25 mm × 30 m ...
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bioRxiv - Immunology 2024Quote: ... in 180 µL for 2 h in a nutrient- and serum-limited growth media composed of Seahorse basal DMEM (cat. no. A1443001, Agilent, Santa Clara, CA, USA) supplemented with 0.5 mM D-glucose (555 mM stock ...
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bioRxiv - Physiology 2024Quote: ... Slides were then incubated with a secondary antibody ([ImmPRESS™ HRP Anti-Goat Ig, Vector, cat# MP-7405] or [HRP Labelled Polymer Anti-Mouse, Dako, cat#K400111-2]) for 30 minutes at RT ...
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bioRxiv - Microbiology 2024Quote: ... 100 μL of this culture was transferred to a 96-well plate and incubated at 37°C in a BioTek Epoch 2 96-well plate reader (Agilent Technologies; Santa Clara, CA) housed within an anaerobic chamber with one minute of shaking prior to an OD600nm measurement every 30 minutes for 24 hours ...
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bioRxiv - Bioengineering 2024Quote: ... The ultrasound focus was aligned to correspond with the optical focus of the microscope as previously described.[68] The 2 MHz frequency ultrasound waves were generated using an arbitrary waveform generator (33220A 20MHz function, Agilent, Palo Alto, CA, USA) and amplified 50 dB (ENI 2100L ...
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bioRxiv - Molecular Biology 2020Quote: ... monoclonal) (Cell Signalling, Danvers, USA), β-actin (#3700, monoclonal) (Cell Signalling, Danvers, USA), and secondary antibodies (P044801-2, polyclonal) from Dako (Agilent, Santa Clara, USA) were used at a dilution 1/1000 (primary ...
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bioRxiv - Physiology 2020Quote: ... Real-time PCR amplification with the 2 x precision master mix (Primer design, Southampton, UK) using the Stratagene Mx3000P real-time QPCR system (Agilent Technologies, Santa Clara, CA, USA). Each sample was tested in triplicate and compared to a housekeeping gene (Atp5B in osteoblasts and bone tissue ...
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bioRxiv - Plant Biology 2022Quote: ... 2 μL of each sample was injected in splitless and split 1:10 mode into a 6890N gas chromatograph (Agilent Technologies Inc. Santa Clara, CA) coupled to a Pegasus 4D TOF mass spectrometer (LECO ...
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bioRxiv - Immunology 2022Quote: ... and a tyramide-barcode staining cycle performed for mouse anti-MUM1 primary antibody (1:100 dilution, clone MUM1p, Agilent Dako Products, catalog number GA64461-2). However ...
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bioRxiv - Molecular Biology 2020Quote: ... and aromatic 1H-13C-NOESY-HSQC experiments with a mixing time of 150 ms each were carried out at 25 °C on a 500 MHz Agilent DirectDrive 2 spectrometer (Agilent Technologies, Santa Clara, CA, USA) equipped with a room temperature probe ...
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bioRxiv - Immunology 2022Quote: ... then incubated in 5 μg/ml primary antibody in incubation buffer (anti-Mala s 1 mouse monoclonal IgG1 antibody (9G9) from Karolinska Institute (Zagari A et al, 1994, Schmidt et al, 1997) or mouse monoclonal IgG1 antibody (Agilent Dako X093101-2; Santa Clara, USA) as an isotype control for 90 min ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed again three times in TBS 1X and immunoreactions were revealed with DAB peroxidase substrate (K346811-2, Agilent Technologies, Santa Clara, CA, USA) was performed ...
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bioRxiv - Microbiology 2024Quote: ... Colorimetric conversion was terminated by addition of 5% SDS (50 uL per well) and absorbance was measured at 414 nm using a BioTek Epoch 2 Microplate Spectrophotometer (Agilent Technologies, Santa Clara, CA, USA). The endpoint absorbance of each sample was determined on a plate-by-plate basis where the absorbances of all the blank wells were averaged and multiplied by three ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed again three times in TBS 1X and immunoreactions were revealed with DAB peroxidase substrate (K346811-2, Agilent Technologies, Santa Clara, CA, USA). Finally ...
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bioRxiv - Biophysics 2023Quote: The samples were shortly centrifuged after thawing and injected onto a cooled (2°C) HPLC System (Agilent Infinity 1260, Agilent Technologies, Santa Clara, CA, USA) which includes a home packed pepsin column (IDEX guard column with 60 μl Porozyme immobilized pepsin beads ...
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bioRxiv - Biochemistry 2023Quote: ... The fluorescence at 590 nm with excitation at 485 nm (F590) was measured with the BioTek Synergy 2 plate reader (Agilent Technologies, Santa Clara, CA, USA). The data were fitted using the Hill equation to estimate parameters from data which has the a priori unknown parameters ...
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bioRxiv - Cancer Biology 2020Quote: ... 4×44K (G4112F, Agilent Technologies, Germany).
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bioRxiv - Cell Biology 2022Quote: ... Insulin (Agilent, IR-002, 1:4), and Glucagon (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and 4% normal goat serum (Dako) in PBS for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... then incubated in 5 μg/ml primary antibody in incubation buffer (anti-Mala s 1 mouse monoclonal IgG1 antibody (9G9) from Karolinska Institute (Zagari A et al, 1994, Schmidt et al, 1997) or mouse monoclonal IgG1 antibody (Agilent Dako X093101-2; Santa Clara, USA) as an isotype control for 90 min ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were washed once with DMEM Assay Medium (XF DMEM [Agilent] supplemented with XF glucose [10 mM, Agilent], XF glutamine [2 mM, Agilent] and XF pyruvate [1 mM, Agilent]), then left in DMEM Assay Medium and placed in a non-CO2 incubator for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal goat anti-rabbit secondary antibody IgG/HRP (P044801-2; Agilent Technologies/Dako, Santa Clara, 527 CA, United States) diluted 1:2000 in milk ...
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bioRxiv - Neuroscience 2024Quote: ... The section was subsequently incubated in primary antibodies (GFAP,1:1000, D1F4Q, Cell Signaling or CD79A, 1:200, M705001-2 DAKO or CD3, 1:100, A0452 DAKO), in BTHP-buffer (1% bovine serum albumin (BSA) ...
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bioRxiv - Biochemistry 2021Quote: ... 4 mL capacity (Agilent Cat. #: 5185-5991) and centrifuged at 17,172 × g for 45 min at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... (4) CD68 (Macrophages, 1:400, M0876; Dako)–Opal 620 ...
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The integrated stress response promotes macrophage inflammation and migration in autoimmune diabetesbioRxiv - Immunology 2024Quote: ... and anti-insulin (Dako; IR002; 1:4) antibodies ...
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bioRxiv - Physiology 2024Quote: ... tissues were incubated with primary antibody over night at 4°C (guinea pig anti-insulin at 1:4, diluted in 1% goat serum/PBS, Agilent). This was followed by 1-hour incubation with fluorophore-conjugated secondary antibodies (Cy3-anti-guinea pig ...
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bioRxiv - Cancer Biology 2024Quote: ... the sections were incubated with primary antibody (PRDX2, TFRC or 4-HNE) at 4°C overnight and followed by incubation with secondary antibody FLEX/HRP (Dako) at room temperature for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... Step 4 was protein blocking (Background Sniper, Dako Protein Block or Normal block ...
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bioRxiv - Cell Biology 2024Quote: ... and anti-insulin antibody (Dako IR002; 1:4). Highly cross-adsorbed Alexa Fluor secondary antibodies (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... diethyl ether and purified on an Agilent 1200 series High-Performance Liquid Chromatography (HPLC) instrument (10–75% HPLC-grade acetonitrile for 20 minutes at a 2 mL/min flow rate over an Agilent Zorbax C18 column (5 µm, 9.4 x 250 mm) tracked at 220 ...
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bioRxiv - Biochemistry 2021Quote: ... at 4°C overnight diluted in antibody diluent (Dako). Negative controls were incubated with antibody diluent only ...
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bioRxiv - Cell Biology 2020Quote: ... elegans V2 4*44K Microarray chip (Agilent Technologies, USA) at 65°C for 17 hrs ...
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bioRxiv - Cancer Biology 2022Quote: ... cytokeratin (DAKO, Cat. M3515; 1:100 at 4 °C), ki67 (ThermoFisher ...