Labshake search
Citations for Agilent :
1351 - 1400 of 2113 citations for 6 cyano 5 methoxy 12 methylindolo 2 3 a carbazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... the labelled antibodies were developed with a Dako Liquid DAB+ substrate chromogen system (Dako; GV82511-2).
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bioRxiv - Bioengineering 2023Quote: ... Slides were then mounted with Dako fluorescence mounting media (S302380-2, Agilent Technologies, Santa Clara, US). The primary antibodies and respective concentrations used in this study are the following ...
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bioRxiv - Cell Biology 2023Quote: ... Optical density (OD) was measured with a BioTek Epoch 2 microplate spectrophotometer (Agilent, Santa Clara, CA) for at least 15 min to obtain blank values for each well at the target temperature ...
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bioRxiv - Neuroscience 2023Quote: ... we mounted the cultures on glass slides with DAKO anti-fading mounting medium (#S302380 − 2, Agilent) for confocal microscope imaging.
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bioRxiv - Bioengineering 2022Quote: ... the sections were buffered in DAKO wash buffer (Cat #K800721-2, Agilent, Santa Clara, CA, USA) before incubating in primary antibody diluted in DAKO antibody diluent (Cat# S080983-2 ...
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bioRxiv - Cancer Biology 2022Quote: ... and anti-CD45 clone 2B11 + PD7/26 (Ready-to-use, Agilent Dako cat. no. GA75161-2). After washing away the primary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... and anti-CD45 clone 2B11 + PD7/26 (Ready-to-use, Agilent Dako cat. no. GA75161-2). After washing away the primary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... The coverslips were then mounted on slides using DAKO Faramount Aqueous Mounting Medium (Agilent, #S302580-2). The slides were kept for 24h to allow the mounting media to cure before use.
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bioRxiv - Cancer Biology 2023Quote: FISH assay was performed on tissue microarrays using the Histology FISH accessory kit (K579911-2, Dako, Agilent Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... we mounted the cultures on glass slides with DAKO anti-fading mounting medium (#S302380 − 2, Agilent) for confocal microscope imaging.
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bioRxiv - Molecular Biology 2023Quote: ... or rabbit anti-goat IgG (500 ng ml−1, 1% BSA in PBST; Agilent, P044901-2), for 1 hour at room temperature ...
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bioRxiv - Genomics 2023Quote: ... then air dried and mounted using an aqueous fluorescence mounting medium (Agilent Dako, Cat# S302380-2). Cells were visualized and imaged using an Olympus UPLXAPO 20×/0.8 NA air objective on a spinning disk confocal microscope (SpinSR10 ...
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bioRxiv - Genomics 2023Quote: ... then air dried and mounted using an aqueous fluorescence mounting medium (Agilent Dako, Cat# S302380-2). Cells were visualized and imaged using an Olympus UPLXAPO 20×/0.8 NA air objective on a spinning disk confocal microscope (SpinSR10 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated 10 minutes in H2O2 solution (S202386-2, Agilent Technologies, Santa Clara, CA, USA) to quench endogenous peroxidases ...
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bioRxiv - Cancer Biology 2024Quote: ... gaskets were removed and slides were mounted onto coverslips using Fluorescence Mounting Medium (S302380-2, Agilent). Images were acquired using a Zeiss Axio Scope.A1 fluorescence microscope with 40× and 100x magnifications and further analyzed with ImageJ ...
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bioRxiv - Cell Biology 2019Quote: ... The anc-1 3’UTR RNAi construct was generated by PCR amplifying part of the 3’UTR-region of the anc-1 transcript using Paq5000™ DNA Polymerase (Agilent Technologies, 600682). The primer sequences are detailed in Table S2 ...
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bioRxiv - Plant Biology 2020Quote: ... The sample components were separated on a C18 olumn (Hypersil GOLD C18; 4.6 mm × 100 mm; 3 µm particle size; Agilent, Santa Cruz, CA, USA). The column was eluted at 0.3 mL/min with a gradient from 95% solvent A (0.1% formic acid in water ...
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bioRxiv - Developmental Biology 2022Quote: ... High quality total RNA (RNA integrity number = 10) was labelled with Cyanine 3 CTP using the Low Input Quick Amp Labelling Kit (Agilent Technologies-5190-2305), and purified using Qiagen’s RNeasy Mini Spin Columns ...
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bioRxiv - Biochemistry 2022Quote: ... All libraries at this step yielded >300,000 colonies implying a 100x coverage (assuming 1/3 of the variants were perfect based on our previous analysis of Agilent OLS based libraries). Each sublibrary was combined at an equimolar ratio to make a complete library with all intended mutations included.
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bioRxiv - Microbiology 2021Quote: ... The extracts were acidified to pH 2 and additionally pre-treated with solid-phase extractions using Agilent Bond Elut-PPL 3 mL columns and diluted to 50 ppm (Agilent Technologies, DE, USA) following standard lab protocol29 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Primary antibodies were diluted 1:4000 for anti-sGCα1 and 1:2000 for anti-sGCβ1 antibody in 3% dry milk in TBST and incubated with nitrocellulose membranes at 4°C over-night following challenge of membranes with secondary goat anti-rabbit antibody (1:2000 in 3% milk in TBST) conjugated to horseradish peroxidase (Dako A/S, Denmark). Immuno-complexes were visualized using an enhanced chemiluminescence kit (Amersham Pharmacia Biotech ...
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bioRxiv - Biochemistry 2022Quote: 50 μL of pure CrRPE1 concentrated at 9.5 mg mL−1 were injected on BioSEC-3 300 size-exclusion chromatography column (Agilent Technologies, Santa Clara, USA) equilibrated in buffer 20 mM Tris-HCl (pH 7.9 ...
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bioRxiv - Biophysics 2022Quote: ... The samples were then further diluted in ultrapure water to a final 3% nitric acid concentration and analyzed by triple quadrupole inductively coupled plasma-mass spectrometry (Agilent 8800 ICP-QQQ). Copper concentrations were determined by using standard curves ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- ACC TCC ACC ACC AGA ACC ACC TCC ACC GCT CTG AAA GTA CAG ATC CTC GGT ACC TTT ATA TAA TTC GTC CAT-3’) using the Agilent QuikChange SDM kit following the manufacturer’s instructions (Agilent, Singapore, Singapore; Cat: STR_210519). This was followed by amplifying the backbone of the plasmid using primer set Ag43BBGib_F ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- CTT TTT CAA ATT GGG GGT GTG ACC ATG CAG CGC TAG CCG CAG C −3’) using the GeneMorph II epPCR kit (Agilent, Singapore, Cat: STR_200550). The epPCR profile is as follows ...
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Age, sex, and cell type-resolved hypothalamic gene expression across the pubertal transition in micebioRxiv - Genomics 2023Quote: RNA-seq libraries were prepared using an automated - QuantSeq 3’mRNA-seq (Lexogen GmbH, Vienna) and Agilent NGS Workstation (Agilent Technologies, Santa Clara) at The Centre for Applied Genomics (TCAG ...
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bioRxiv - Molecular Biology 2019Quote: ... The mixture was pre-incubated at 37°C for 5 min and AffinityScript Multiple Temperature Reverse Transcriptase (Agilent Technologies) was added to each toeprinting reaction at a final content of 10.2% (v/v ...
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bioRxiv - Evolutionary Biology 2021Quote: ... An Agilent J&W HP-5 column (30 m × 0.25 mm i.d., 0.25 μm film thickness; Agilent Technologies, USA) was used in the GC ...
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bioRxiv - Immunology 2022Quote: ... the membrane was incubated in secondary antibody solutions (TBS containing 0.1 % Tween-20 and 5 % BSA, HRP-conjugated secondary antibodies (1:5000, Dako)) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were desalted online by reversed-phase chromatography on a Poroshell 300SB C3 column (1.0 × 75 mm, 5 μm, Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Separation occurred with a HP-5 ms capillary column coated with 5% phenyl-95% methylpolysiloxane (30 m×250 µm i.d., 0.25 µm film thickness, Agilent Technologies).
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bioRxiv - Molecular Biology 2019Quote: EMSAs were performed as described and cross-linked for 5 min (∼720 mJ) in a UV Stratalinker 1800 (Stratagene). Samples were taken up in 2x SDS loading buffer and boiled for 5 min before being loaded on a 7.5 % denaturing polyacrylamide gel and run for 1-2 hours in 10 % SDS-running buffer at 130 V ...
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bioRxiv - Microbiology 2019Quote: ... equipped with an HP-5 capillary column (60 m × 0.25 mm × 0.25 μm, Agilent Technologies, Inc., Santa Clara, CA). Pure helium (99.9999 % ...
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bioRxiv - Plant Biology 2019Quote: ... An HP-5MS capillary column (5%phenyl-methyl-siloxane, 30-m, 250-mm, and 0.25-mm film thickness; Agilent) was used with helium carrier gas at 2 mL/min ...
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bioRxiv - Synthetic Biology 2020Quote: ... Ten microliters of each diluted sample were injected and sample separation was achieved using a Zorbax Eclipse Plus C18 guard column (4.6 cm×12.5 cm, 5 μm packing, Agilent Technologies) connected to a Zorbax Eclipse Plus C18 column (4.6 mm × 100 mm ...
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bioRxiv - Cancer Biology 2021Quote: ... the TMT-labelled peptides were fractionated using high pH reverse phase chromatography on a C18 column (150 × 2.1 mm i.d. - Kinetex EVO (5 μm, 100 Å)) using an HPLC system (Agilent, LC 1260 Infinity II ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 μg of pooled total RNA were tested for RNA quality on an ABI2000 Bioanalyzer (Agilent; Santa Clara, CA), labeled ...
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bioRxiv - Biochemistry 2020Quote: Concentrated protein samples were diluted to 0.02 mg/mL in 0.1% formic acid and 50 μL was injected on to a 2.1 mm x 12.5 mm Zorbax 5 μm 300SB-C3 guard column (Agilent Technologies) housed in a column oven set at 40°C ...
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bioRxiv - Microbiology 2021Quote: ... with a 30 x 0.25 mm (0.25 µm thickness) DB-5 MS capillary column (Agilent J & W capillary GC) and coupled to a Polaris Q benchtop Ion Trap Mass Spectrometer ...
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bioRxiv - Biophysics 2020Quote: ... in 20 min at a flow rate of 0.4 mL min-1 using a Zorbax Narrow Bore (2.1×50 mm, 5 μm) C18 column (Agilent Technologies).
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bioRxiv - Cancer Biology 2022Quote: ... HPTS and RuBPY fluorescence were monitored for 17 h using a Cytation 5 device (BioTek, Agilent, Winooski, VT, USA). Excitation was provided by a monochromator ...
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bioRxiv - Cancer Biology 2022Quote: ... and read by brightfield and fluorescence imaging on a BioTek Cytation 5 Cell Imaging Multi-Mode Reader (Agilent Technologies) every six hours for a total of six days ...
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bioRxiv - Biochemistry 2022Quote: ... The gas chromatograph was equipped with a DB-5 ms column (30 m × 0.25 mm, film thickness 0.25 μm; including 10 m DuraGuard column, Agilent Technologies) and a GC inlet liner (ultra inert ...
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bioRxiv - Synthetic Biology 2022Quote: ... fitted with a guard column (Zorbax Eclipse XDB-C18, 2.1 x 5 mm 1.8 μm, Agilent part # 821725-903) using a 1290 Infinity II UHPLC (G7120AR ...
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bioRxiv - Molecular Biology 2022Quote: ... pH 9.0 for 30 minutes followed by a 5-minute incubation in DAKO Peroxidase Block (DAKO, Catalog No. S2001) to block endogenous peroxidase activity ...
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bioRxiv - Plant Biology 2021Quote: Quantitative analysis was performed using a HP-5MS capillary column (5% phenyl-methyl-siloxane, 30 m x 250 mm, 0.25 mm film thickness; Agilent) with helium carrier gas at 1.5 mL/min ...
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bioRxiv - Microbiology 2021Quote: ... samples (5 uL) were injected onto a ZORBAX RRHD Extend-C18 column (2.1 x 150 mm, 1.8 µm; Agilent Technologies) with a ZORBAX SB-C8 (2.1 mm × 30 mm ...
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bioRxiv - Neuroscience 2020Quote: ... and loaded onto a ZORBAX Extend 300 C18 columns (4.6 mm x 250 mm with 5 µm beads) from Agilent. An Agilent 1100 HPLC system was used to carry out the fractionation ...
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bioRxiv - Microbiology 2022Quote: ... containing peptaibols was carried out by semi-preparative HPLC on an SSI system (Teledyne SSI Lab Alliance Series III pump system and Series 1500 Photodiode Array Detector) with an Eclipse XDB-C18 column (9.4 × 250 mm, 5 μm, Agilent). Acetonitrile (A ...
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bioRxiv - Microbiology 2022Quote: ... After centrifugation plate was incubated for 5 minutes before being loaded into a Seahorse XF96 Extracellular Flux Analyzer (Agilent). Basal respiration was measured over six hours with 36 ten-minute protocol cycles including a ...