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Citations for Agilent :
1351 - 1400 of 2557 citations for 2 Amino 6 chloro 9 3 5 di O p toluoyl beta D 2 deoxyribofurnanosyl purine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: All AAV (serotype 2) vectors were produced by triple transfection of 293 cells using a rep/cap plasmid and pHelper (Stratagene, La Jolla CA, USA), and purified by column affinity chromatography as previously described38 ...
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bioRxiv - Cancer Biology 2021Quote: ... qRT-PCR was performed as previously described (Sodi et al., 2015) using Brilliant II qRT-PCR Master Mix 2 Kit (Stratagene, San Diego, CA, USA) using Applied Biosystems 7500 machine. ...
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bioRxiv - Synthetic Biology 2020Quote: ... The hybridisation mix was prepared by mixing the specified amounts of RNA with 10× Gene Expression Blocking Agent (1× final conc.) and 2× Hi-RPM Hybridization Buffer (1× final conc.) (Agilent, Cat No. 5188-5242) in a final volume of 42 μl ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight at 4°C in primary antibody (2% NGS in TBST) with either rabbit anti-GFAP (1:2000, Agilent Cat# Z0334, RRID: AB_10013382), rabbit anti-synaptophysin (IgG ...
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bioRxiv - Immunology 2021Quote: ... The size of the amplicon was confirmed by analyzing 2 μl of PCR products using the Agilent D5000 ScreenTape System (Agilent D5000 ScreenTape, 5067-5588, Agilent D5000, Reagents 5067-5589). Products were quantified by analyzing 2 μl with the Quant-iT dsDNA High-Sensitivity Assay Kit (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: Fraction HMO-2 was digested by treatment of 10 µg dried sample with β-galactosidase from bovine testes (10 mU, Prozyme, Invitrogen, Karlsruhe, Germany) in 20 µl of 0.1 M citrate/phosphate buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated for 20 minutes in a microwave oven in DAKO Target Retrieval pH6 (S203130-2, DAKO, Agilent Technologies, Santa Clara, CA, USA). Then ...
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bioRxiv - Microbiology 2023Quote: ... One microliter of each methoximated and trimethylsilylated sample was injected by 2:1 split injection by an Agilent Technologies 7693 autosampler (Agilent Technologies, Santa Clara; California) into an Agilent 7890A gas chromatograph equipped with a fused silica capillary column (0.25 mm × 30 m ...
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bioRxiv - Molecular Biology 2024Quote: ... 2-4 images at the central callus and distal callus regions were taken using an automatic microscope (Agilent, BioTek Lionheart FX, Santa Clara, CA). Central callus regions were defined as proximal to the fracture site on either side of the bone ...
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bioRxiv - Synthetic Biology 2022Quote: ... Growth experiments were conducted (either at 100% air or 90% air / 10% CO2) in a BioTek Epoch 2 plate reader (Agilent, Santa Clara, CA, USA) at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... 100 μL of this culture was transferred to a 96-well plate and incubated at 37°C in a BioTek Epoch 2 96-well plate reader (Agilent Technologies; Santa Clara, CA) housed within an anaerobic chamber with one minute of shaking prior to an OD600nm measurement every 30 minutes for 24 hours ...
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bioRxiv - Immunology 2024Quote: ... in 180 µL for 2 h in a nutrient- and serum-limited growth media composed of Seahorse basal DMEM (cat. no. A1443001, Agilent, Santa Clara, CA, USA) supplemented with 0.5 mM D-glucose (555 mM stock ...
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bioRxiv - Physiology 2024Quote: ... Slides were then incubated with a secondary antibody ([ImmPRESS™ HRP Anti-Goat Ig, Vector, cat# MP-7405] or [HRP Labelled Polymer Anti-Mouse, Dako, cat#K400111-2]) for 30 minutes at RT ...
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bioRxiv - Bioengineering 2024Quote: ... The ultrasound focus was aligned to correspond with the optical focus of the microscope as previously described.[68] The 2 MHz frequency ultrasound waves were generated using an arbitrary waveform generator (33220A 20MHz function, Agilent, Palo Alto, CA, USA) and amplified 50 dB (ENI 2100L ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-Krt5/6 (1:200; M7237; Agilent; Santa Clara, CA), and mouse anti-Krt14 (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen was retrieved using DakoCytomation target retrieval solution pH 6 (Dako). Samples were then blocked with serum-free protein block solution (Dako ...
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bioRxiv - Microbiology 2020Quote: ... and p-dsbS(S239A)- flag were obtained using the QuikChange II site-directed mutagenesis kit (Stratagene, catalog#: 200518). For generating p-dsbR(D51A)-flag ...
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bioRxiv - Cancer Biology 2023Quote: ... and MET p.(Cys1125Gly) were created with the QuickChange site-directed mutagenesis system (Agilent Technologies, Santa Clara, CA) on the pRS2 vector encoding wild-type MET cDNA ...
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Machine learning and data-driven inverse modeling of metabolomics unveil key process of active agingbioRxiv - Systems Biology 2024Quote: ... Gas separation was performed on the HP-5MS column (30 m 3 0.25 mm 3 0.25 mm, Agilent Technologies).
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bioRxiv - Biochemistry 2021Quote: ... amino acid substitutions were introduced using a QuikChange II XL site directed mutagenesis kit (200521) purchased from Agilent (Santa Clara, CA). After transformation into BL21-Gold (DE3 ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids harboring GlnR amino acid substitutions (pET28a-glnR derivatives) were constructed using site-directed mutagenesis (QuikChange Site-Directed Mutagenesis Kit, Agilent, Inc.).DNA fragments consisting of different canonical GlnR-dependent promoters (narG whose downstream genes encoding M ...
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bioRxiv - Molecular Biology 2020Quote: ... monoclonal) (Cell Signalling, Danvers, USA), β-actin (#3700, monoclonal) (Cell Signalling, Danvers, USA), and secondary antibodies (P044801-2, polyclonal) from Dako (Agilent, Santa Clara, USA) were used at a dilution 1/1000 (primary ...
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bioRxiv - Physiology 2020Quote: ... Real-time PCR amplification with the 2 x precision master mix (Primer design, Southampton, UK) using the Stratagene Mx3000P real-time QPCR system (Agilent Technologies, Santa Clara, CA, USA). Each sample was tested in triplicate and compared to a housekeeping gene (Atp5B in osteoblasts and bone tissue ...
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bioRxiv - Plant Biology 2022Quote: ... 2 μL of each sample was injected in splitless and split 1:10 mode into a 6890N gas chromatograph (Agilent Technologies Inc. Santa Clara, CA) coupled to a Pegasus 4D TOF mass spectrometer (LECO ...
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bioRxiv - Immunology 2022Quote: ... and a tyramide-barcode staining cycle performed for mouse anti-MUM1 primary antibody (1:100 dilution, clone MUM1p, Agilent Dako Products, catalog number GA64461-2). However ...
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bioRxiv - Molecular Biology 2020Quote: ... and aromatic 1H-13C-NOESY-HSQC experiments with a mixing time of 150 ms each were carried out at 25 °C on a 500 MHz Agilent DirectDrive 2 spectrometer (Agilent Technologies, Santa Clara, CA, USA) equipped with a room temperature probe ...
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bioRxiv - Biophysics 2023Quote: The samples were shortly centrifuged after thawing and injected onto a cooled (2°C) HPLC System (Agilent Infinity 1260, Agilent Technologies, Santa Clara, CA, USA) which includes a home packed pepsin column (IDEX guard column with 60 μl Porozyme immobilized pepsin beads ...
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bioRxiv - Microbiology 2024Quote: ... Colorimetric conversion was terminated by addition of 5% SDS (50 uL per well) and absorbance was measured at 414 nm using a BioTek Epoch 2 Microplate Spectrophotometer (Agilent Technologies, Santa Clara, CA, USA). The endpoint absorbance of each sample was determined on a plate-by-plate basis where the absorbances of all the blank wells were averaged and multiplied by three ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed again three times in TBS 1X and immunoreactions were revealed with DAB peroxidase substrate (K346811-2, Agilent Technologies, Santa Clara, CA, USA). Finally ...
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bioRxiv - Immunology 2022Quote: ... then incubated in 5 μg/ml primary antibody in incubation buffer (anti-Mala s 1 mouse monoclonal IgG1 antibody (9G9) from Karolinska Institute (Zagari A et al, 1994, Schmidt et al, 1997) or mouse monoclonal IgG1 antibody (Agilent Dako X093101-2; Santa Clara, USA) as an isotype control for 90 min ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed again three times in TBS 1X and immunoreactions were revealed with DAB peroxidase substrate (K346811-2, Agilent Technologies, Santa Clara, CA, USA) was performed ...
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bioRxiv - Biochemistry 2023Quote: ... The fluorescence at 590 nm with excitation at 485 nm (F590) was measured with the BioTek Synergy 2 plate reader (Agilent Technologies, Santa Clara, CA, USA). The data were fitted using the Hill equation to estimate parameters from data which has the a priori unknown parameters ...
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bioRxiv - Physiology 2020Quote: ... Acetone was measured using an agilent DB-35MS column (30 m 3 0.25mm i.d. 3 0.25 mm, Agilent J&W Scientific) installed in an Agilent 7890A gas chromatograph (GC ...
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bioRxiv - Biophysics 2024Quote: ... using a 3 × 3 mm black walls quartz cell at 25 °C on an Agilent Cary Eclipse spectrofluorometer (Agilent Technologies) equipped with a thermostatted cell holder attached to an Agilent PCB 1500 water Peltier system ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-human CD20 (Dako, clone L26, 1:1000, pH 6 retrieval), mouse anti-human FOXP3 (Abcam ...
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bioRxiv - Genomics 2020Quote: ... Each pool (2nM per library; Agilent SureSelect XT HS, n=6; Agilent SureSelect XT RNA Direct ...
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bioRxiv - Biochemistry 2023Quote: ... Antigen retrieval was performed with Citrate Buffer (pH 6) (Dako, Glostrup, Denmark). Immunohistochemical staining was performed with anti VEGFA or anti-S100A8 (Table I) ...
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bioRxiv - Molecular Biology 2022Quote: ... Fragment 3 was pBluescript KS (-) (Stratagene, California USA), which was digested using Xba ...
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bioRxiv - Pathology 2021Quote: ... 3′-diaminobenzidine tetra-hydrochloride chromogen (DAB, K3468, Dako) was added to all sections and the reaction was stopped with distilled water ...
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bioRxiv - Microbiology 2023Quote: ... then labeled using Cyanin-3 CTP (Agilent Technologies) and hybridized on chips containing 45000 probes (4×44K Whole Human Genome) ...
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bioRxiv - Microbiology 2023Quote: ... 3 × 150 mm 2.7 μm HPLC column (Agilent) with a Poroshell 120 PFP ...
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bioRxiv - Microbiology 2022Quote: ... 3’ Diaminobenzidine (DAB) solution (Dako Agilent Pathology Solutions) was applied for eight minutes ...
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bioRxiv - Microbiology 2022Quote: ... 3’ Diaminobenzidine (DAB) solution (Dako Agilent Pathology Solutions) was applied for eight minutes ...
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bioRxiv - Microbiology 2024Quote: ... anti-Cluster of Differentiation 3 (CD3; Agilent Dako), anti-Paired box protein-5 (Pax-5 ...
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bioRxiv - Microbiology 2024Quote: ... anti-Cluster of Differentiation 3 (CD3; Agilent Dako), anti-Paired box protein-5 (Pax-5 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... p-nitrophenyl acetate (pNPA) hydrolysis was determined using a SpectraMax Plus384 microplate reader (Agilent Technologies, Seoul, Republic of Korea). The cell lysates were transferred to a 96-well plate and reactions (0.2 mL final mixture volume ...
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bioRxiv - Microbiology 2021Quote: Plasmids encoding the single-mutation variants found in P.1 and 10-mutation variant (BZΔ10) were generated by Quikchange II XL site-directed mutagenesis kit (Agilent). Recombinant Indiana VSV (rVSV ...
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bioRxiv - Bioengineering 2023Quote: ... After calibrating and verifying the performance of the column using the LC Bio-standard (Agilent, P/N : 5190-9417), the recombinant antibody samples were diluted to 2mg/ml in 1XPBS and loaded onto a pre-conditioned column at a flow rate of 0.35ml/min ...
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bioRxiv - Cell Biology 2024Quote: ... Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) and Rotenone/Antimycin A was measured using the mitochondrial stress test kit (Agilent, USA). The glycolytic activity or glycoPER in response to Rotenone/Antimycin A and 2-deoxy-D-glucose was measured using glycolytic rate assay (Agilent ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μl C18 Cartridges (Agilent) were used as solid phase ...