Labshake search
Citations for Agilent :
1301 - 1350 of 3808 citations for 8 Chloro 4 methyl 1 2 dihydroquinolin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Samples were washed three times with PBS and one time with distilled water and mounted with Dako Fluorescent Mounting Medium (Dako, Denmark). Samples were visualized in the epifluorescence microscope Olympus BX53 fluorescence microscopy with UPLFLN 100× oil objective (numerical aperture 1.30) ...
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bioRxiv - Genetics 2023Quote: ... Cyanine-3 (Cy3)-labeled cRNAs were prepared with 100ng of total RNA using a One-Color Low Input Quick Amp Labeling kit (Agilent Technologies). The specific activities and cRNA yields were determined by using a NanoDrop ND-1000 (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: ... Total RNA was quantified using a NanoDropTM One spectrophotometer and quality assessed with an Agilent 2100 Bioanalyser using an RNA chip (Agilent Technologies). For library construction all RNA samples had a RIN value >7 ...
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bioRxiv - Molecular Biology 2020Quote: ... Final library yields and size were measured on a Bioanalyzer 2100 using high sensitivity reagents (Agilent Technologies, Supplementary Figure 8).
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bioRxiv - Cancer Biology 2021Quote: ... Endogenous peroxidases were blocked with peroxidase inhibitor (CM1) for 8 minutes before incubating the section with CD3 antibody (Dako, #A0452) at 1:300 dilution for 60 minutes at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were washed for 5 minutes using 1X Wash Buffer and then blocked by adding 8 drops of Protein Block Serum-Free (Agilent). Primary antibodies were diluted in diluent (Dako ...
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bioRxiv - Cell Biology 2019Quote: ... Agilent gene expression microarray 60K slide (Design ID: 72363. SurePrint G3 Human Gene Expression 8 × 60K Microarray Kit, Agilent Technologies) were used ...
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bioRxiv - Genomics 2019Quote: ... Whole genome expression data was obtained using Agilent Sureprint G3 Mouse Gene Expression 8×60K microarrays (Agilent Technologies cat.# G4852B) with Low Input Quick Amp Labeling protocol (Agilent Technologies cat.# 5190-2331 ...
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bioRxiv - Molecular Biology 2019Quote: ... Sample input was 900ng total RNA (determined by Qubit RNA HS reagents, Thermo) with a RIN >8 (TapeStation High Sensitivity RNA ScreenTape Assay, Agilent). RNA was fragmented for 15 minutes and libraries were constructed with a 1/5th dilution of NEB adaptor and 12 cycles of PCR amplification with dual-indexing primers ...
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bioRxiv - Genetics 2019Quote: ... UV Crosslinker, CL-1000, 254 nm, 100 V, 8 W [UVP/Analytik Jena] or Stratalinker UV crosslinker Model 1800 [Stratagene]). Under these conditions ...
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bioRxiv - Systems Biology 2019Quote: ... An amount of 100 ng of total RNA was hybridised with Cy-3 and subsequently hybridised on microarrays (SurePrint G3 8×60K, Agilent). The raw fluorescence signals were scanned with DNA Microarray Scanner SureSelect (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... Hydrolyzed amino acids were separated using ultra performance liquid chromatography (UPLC, Acquity, Waters) on a C-8 column (Zorbax Eclipse XBD, Agilent) at a flow rate of 0.6 mL/min ...
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bioRxiv - Cell Biology 2021Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8 × 60K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Systems Biology 2021Quote: ... respectively) on a 300 × 7.7 mm PL Hi-Plex H+ (8 µm particle size) column (Agilent Technologies, Santa Clara, CA) and 4 x 3 mm ID Carbo-H guard (Phenomenex ...
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bioRxiv - Cell Biology 2022Quote: ... The Cy3-labeled DNA was hybridized overnight to 8×60K 60mer oligonucleotide spotted microarray slides (Agilent Technologies, design ID 074809). For each protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The Cy3-labeled DNA was hybridized overnight to 8×60K 60mer oligonucleotide spotted microarray slides (Agilent Technologies, design ID 028005). For experiments where samples yielded <50 ng/μl of RNA ...
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bioRxiv - Neuroscience 2022Quote: ... A frequency of 59.673 MHz and an amplitude of 8 dBm was generated by a signal generator (EXG Analog Signal Generator, NS171B, 9 kHz – 3 GHz, Agilent) and fed into the transmitter and reception boards of the scanner via a respective custom made power splitter.5
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bioRxiv - Genomics 2022Quote: ... The library was amplified using 8 PCR cycles and verified on a Fragment Analyzer using the HS NGS fragment kit (Agilent). The library was quantified by qPCR using the KAPA Library quantification kit (Roche ...
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Local assembly of long reads enables phylogenomics of transposable elements in a polyploid cell linebioRxiv - Genomics 2022Quote: ... Purified DNA was precipitated by addition of 8 mL of ethanol and resuspended in TE buffer and size was analyzed by TapeStation (Agilent) prior to library preparation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A 20 µl sample was injected into the Hi-Plex H column (300×7.7 mm; 8 µm particle size, Agilent Tech.). 0.1% formic acid in Milli-Q water (Merck Millipore ...
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bioRxiv - Cancer Biology 2020Quote: Microarray was performed on in vitro cultures of dMMR and CIN MC38 CRC cells using the SurePrint G3 Mouse Gene Expression v2 Microarray (8×60K) (Agilent) system ...
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bioRxiv - Microbiology 2022Quote: The bacterial transcriptome was interrogated using a custom SurePrint G3 GE 8×60K chip designed in house and produced by Agilent Technologies (4) ...
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bioRxiv - Cancer Biology 2019Quote: ... For cDNA expression microarray analysis using SurePrint G3 Human GE 8×60K Ver.2.0 (Agilent Technologies, Santa Clara, CA, USA), total RNA was sent to a commercial service (Hokkaido System Science ...
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bioRxiv - Immunology 2020Quote: ... RNA sample quality control assessment (RNA integrity number ≥8) was performed with the RNA Pico 6000 chip on Bioanalyzer 2100 (Agilent) and were quantified with Qubit Fluorometer (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2021Quote: Microarray analysis was performed on six control and six IL1B samples (O4+ cells isolated at P5 after in vivo PBS or IL1B treatment) using Agilent Whole Mouse Genome Oligo Microarrays 8×60K (Agilent). Raw data are available in Dataset S2 ...
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bioRxiv - Systems Biology 2020Quote: ... The resulting Cy3-labeled cRNA was hybridized along with a uniform Cy5-labeled reference sample (as an internal standard) to custom gene-specific 8 x 60k oligonucleotide Agilent arrays (Agilent design ID ...
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bioRxiv - Cell Biology 2019Quote: ... and RNA quality was determined using a Bioanalyzer 2100 (Agilent; RIN>8, OD 260/280 and OD 260/230>1.8). RNA libraries were prepared using the TruSeq Stranded mRNA Library Preparation Kit (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... Nucleosomal DNA libraries were pooled in groups of 8 per lane and quantified with by TapeStation (Agilent 2200 TapeStation system) run using a D1000 ScreenTape (Agilent) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were seeded at 2.5 x 104 cells/well into 8-well Seahorse XFp cell culture miniplates (Agilent Technologies, USA) and treated with the sialyltransferase inhibitor for 48 hours ...
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bioRxiv - Synthetic Biology 2022Quote: ... 10 µl of protein sample in assay buffer (20 mM sodium phosphate, 20 mM sodium chloride, pH 8) was injected onto a C4 column (Agilent) and eluted in a water (1% FA ...
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bioRxiv - Neuroscience 2022Quote: ... We assessed the integrity of RNA by the presence of well-defined 28S and 18S ribosomal bands and RNA integrity number ≥ 8 using the 2100 bioanalyzer (Agilent). Reverse transcription (RT ...
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bioRxiv - Cell Biology 2023Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8 × 60K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Developmental Biology 2023Quote: ... A total of 10×103 ESCs and 8×103 EpiLCs were seeded on Seahorse XF96 plates (101085-004, Agilent Technologies) and exposed to increasing doses of Δ9-THC for 48h ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA samples were purified with Ampure XP beads at a 1:0.8 ratio and cDNA quality in randomly selected 10 wells/plate was assessed on a High Sensitivity Bioanalyzer chip (Agilent). cDNA concentrations were quantified using Quant-it PicoGreen dsDNA Assay kit (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 2000 cells were seeded on a well of a 96-well plate and brightfield images were taken every two hours by using the BioTek BioSpa 8 Automated Incubator and Citation 5 reader (Agilent). BioTek Gen5 imaging software (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were purified with Ampure XP beads at a 1:0.8 ratio and DNA quality in randomly selected 10 wells/plate was assessed on a High Sensitivity Bioanalyzer chip (Agilent). Libraries were quantified as mentioned above and normalized libraries were equimolarly pooled and purified one last time at a 1:0.8 ratio ...
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bioRxiv - Immunology 2023Quote: ... Peptide digests were reconstituted in 0.1% Formic Acid/ddH2O at 0.1 µg/µL and then injected (8 µL each) onto a 1260 Infinity nHPLC stack (Agilent Technologies) and separated using a 75 µm I.D ...
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bioRxiv - Cancer Biology 2023Quote: ... Number of pre-capture PCR cycles was 8 - 14 based on input DNA quantity and quality (assessed by Agilent TapeStation) as advised by the manufacturers’ protocol and run on an AB Veriti 96-well Thermocycler (Applied Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... 8 and 12 h and K+ concentration in the sample was determined by ICP-OES (Agilent 710, Agilent Technologies, USA) as described previously (Cai et al. ...
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bioRxiv - Plant Biology 2023Quote: ... 8 and 12 h and K+ concentration in the sample was determined by ICP-OES (Agilent 710, Agilent Technologies, USA) as described previously (Cai et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... at a ratio of 0.8:1 beads:cDNA (20 μl beads:25 μl cDNA for Smart-seq2 and 8 μl beads:10 μl cDNA for Smart-seq3) and inspected on a Bioanalyzer 2100 (High Sensitivity DNA kit, Agilent).
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bioRxiv - Microbiology 2023Quote: ... The sample was passed up and down through a 29-gauge needle 6-8 times and the fragment size distribution was determined (∼30 kbp; TapeStation, Agilent).
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bioRxiv - Microbiology 2021Quote: ... Subsequent antibody incubation was performed at 4°C overnight or for one hour at room temperature using Dako polyclonal goat anti-rabbit or anti-mouse immunoglobulins/HRP (Agilent Technologies, USA). Membranes were washed using 0.1% TBS-T and proteins were detected using ECL or ECL Select (GE Healthcare ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were washed twice with PBS + 0.5% Triton X-100 and then rinsed one time with PBS before mounting with DAKO Fluorescence Mounting Medium (S3023; Dako NA Inc.). Images were analyzed with a Nikon Eclipse Ti fluorescence microscope with a Plan Fluor 40 Å∼ /1.30 Oil DIC N2 objective ...
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bioRxiv - Immunology 2020Quote: mRNA in total RNA was converted to cDNAby using AffinityScript One-Step RT-PCR Kit (Cat# 600559, Agilent, Santa Clara, CA, USA) following the method described elsewhere (17) ...
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bioRxiv - Developmental Biology 2023Quote: ... one µg of total RNA was used to synthetize cDNAs using oligo(dT) primers and affinity script reverse transcriptase (Agilent technologies France). Real-time quantitative PCR analyses were performed using the Light Cycler LC 1.5 (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 µm tissue sections were stained and developed using AutostainerPlus (Dako). Antibodies were diluted in block solution and sections were incubated for 30 minutes with primary antibody and 20 minutes with secondary antibodies ...
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bioRxiv - Synthetic Biology 2020Quote: ... or Streptococcus pneumoniae β-N-Acetylhexosaminidase (Prozyme, 4 mU per digest) overnight at 37°C in 20 mM sodium acetate (pH 5.0).
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bioRxiv - Cancer Biology 2019Quote: ... 4°C hold) by Herculase II fusion enzyme (Agilent Technologies, #600679). After each step DNA was purified by Ampure XP beads (Beckmann Coulter Genomics #A63882) ...