Anti TOM34 Antibody citations for Agilent

Quote: ... One μg/ml GFP antibody (Nacali Tesque Inc., cat# GF200) and 0.5 μg/ml HRP-conjugated mouse antibody (Dako, cat# P0447) were used for detecting DNA-binding proteins ...

Progressive axonopathy when oligodendrocytes lack the myelin protein CMTM5

Tobias J. Buscham, et al., bioRxiv - Neuroscience 2021

Quote: ... TUJ1 (Covance) and CMTM5 (custom made by Pineda, Berlin, Germany Table 2) antibodies were diluted in antibody diluent (DAKO, Hamburg, Germany) and incubated for 48 hours at 4°C in a dark ...

Autologous adipose-derived stem cell transplantation enhances healing of wound with exposed bone in a rat model

Tomo Hamada, et al., bioRxiv - Animal Behavior and Cognition 2019

Quote: ... Primary anti-CD31 antibody in Tris-HCl buffer containing stabilizing protein and 0.015 mol/L sodium azide (Dako Antibody Diluent, Dako, Glostrup, Denmark) were then added to the slides ...

Iron promotes Slc7a11-deficient valvular interstitial cell osteogenic differentiation: A possible mechanism by which ferroptosis participates in intraleaflet hemorrhage-induced calcification

Ran Xu, Dan Zhu, Jianghong Guo, Ying Huang, bioRxiv - Molecular Biology 2021

Quote: ... The sections were incubated with a primary antibody at 4°C overnight and with a rabbit/mouse HRP-conjugated secondary antibody (Dako, Denmark) at room temperature for 2 h ...

Mitochondrial dysfunction compromises ciliary homeostasis in astrocytes

Olesia Ignatenko, et al., bioRxiv - Neuroscience 2022

Quote: ... Samples were then incubated overnight at +4 °C with primary antibodies diluted in the same solution or in an antibody diluent (Agilent #S3022). The samples were then washed in 0.1 % Triton X-100 or 1.0 % Tween20 in PBS for 30-60 minutes ...

Activation of MrgprA3 and MrgprC11 on bladder-innervating afferents induces peripheral and central hypersensitivity to bladder distension

Luke Grundy, et al., bioRxiv - Neuroscience 2020

Quote: ... to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK; 1:800) in Antibody Diluent (S0809, Agilent DAKO). Following removal of excess primary antibody with wash buffer ...

Single-cell RNA sequencing reveals distinct tumor microenvironmental patterns in lung adenocarcinoma

Philip Bischoff, et al., bioRxiv - Cancer Biology 2020

Quote: ... Sections were incubated with primary antibodies for 60 min and with secondary antibodies for 30 minutes at room temperature diluted in Dako Real Antibody Diluent (Dako, S2022). The following primary antibodies were used ...

Intraperitoneal oil application causes local inflammation with depletion of resident peritoneal macrophages

Elisenda Alsina-Sanchis, et al., bioRxiv - Pathology 2020

Quote: ... Primary antibodies were incubated overnight at 4°C and appropriate HRP-conjugated secondary antibodies (DAKO, Agilent Technologies, Santa Clara, CA, USA) for 1 hour at room temperature ...

m⁶A regulates the stability of cellular transcripts required for efficient KSHV lytic replication

Oliver Manners, et al., bioRxiv - Microbiology 2023

Quote: ... skimmed milk powder and probed with desired primary antibodies and secondary horseradish peroxidase conjugated IgG antibodies at 1:5000 (Dako Agilent). Protein bands were detected using a G:BOX (Syngene ...

Increased beta2-adrenergic signaling is a targetable stimulus essential for bone healing by promoting callus neovascularization

Denise Jahn, et al., bioRxiv - Cell Biology 2023

Quote: ... frozen femur sections were blocked with 3% BSA and 5% goat serum in PBS, incubated with Cgrp antibody (rabbit polyclonal, abcam #ab47027, 1:300 in DAKO antibody diluent (Agilent, #S0809)) ...

Pericyte ablation causes hypoactivity and reactive gliosis in adult mice

Jake M. Cashion, et al., bioRxiv - Neuroscience 2023

Quote: ... for 60 min and incubated overnight at 4°C with primary antibodies diluted in DAKO Antibody Diluent (Agilent, cat. #S080983-2). Sections were washed in PBS (3 x 5 min ...

Dormancy-inducing 3D-engineered matrix uncovers mechanosensitive and drug protective FHL2-p21 signaling axis

Sadra Bakhshandeh, et al., bioRxiv - Bioengineering 2024

Quote: ... Primary (FHL2 2 µg/mL and Ki67 2 µg/mL) and secondary antibodies (same as for in vitro immunofluorescence staining) were diluted in Dako antibody diluent (Dako, Germany). Washing steps were done in Dako washing buffer ...

Discovery of conformation-sensitive anti-amyloid protofibril monoclonal antibodies using an engineered chaperone-like amyloid-binding protein

W Vallen Graham, et al., bioRxiv - Molecular Biology 2019

Quote: ... The sections were then incubated for 45 min room temperature in the respective primary antibody diluted in antibody diluent (Dako, Cat. # S3022) and the signal detected using a HRP-DAB based detection system kit (Dako ...

RESTORING GLUTAMATE RECEPTOR SIGNALING IN PANCREATIC ALPHA CELLS RESCUES GLUCAGON RESPONSES IN TYPE 1 DIABETES

Julia K. Panzer, et al., bioRxiv - Physiology 2021

Quote: ... Primary antibodies are diluted in 1x blocking solution and incubated with antibodies against insulin (1:2000, guineapig, Dako, cat. no. A-0546), glucagon (1:500 ...

PU.1 drives specification of pluripotent stem cell-derived endothelial cells to LSEC-like cells

Jonathan De Smedt, et al., bioRxiv - Developmental Biology 2020

Quote: ... and incubated for 30 minutes at room temperature with the secondary antibody (Supplementary Table 2)(1:500 diluted in DAKO Antibody Diluent). Cells were washed with 0.2% Triton X-100 in PBS ...

Neutrophil extracellular traps impair regeneration

Eric Wier, et al., bioRxiv - Developmental Biology 2021

Quote: ... Tissue sections were then incubated overnight at 4°C with primary antibodies (Extended Table 2) in Antibody Diluent (Agilent Dako, S080983-2). Following a wash step ...

Neutrophil extracellular traps impair regeneration

Eric Wier, et al., bioRxiv - Developmental Biology 2021

Quote: ... Tissue sections were then incubated overnight at 4°C with primary antibodies (Extended Table 2) in Antibody Diluent (Agilent Dako, S080983-2). Following a wash step ...

Neuron-specific increase in lamin B1 disrupts nuclear function in Huntington’s disease

Rafael Alcalá-Vida, et al., bioRxiv - Neuroscience 2020

Quote: ... three washes with phosphate buffered saline (PBS) and the incubation with the primary antibody in the DAKO Real TM antibody diluent (Agilent #S202230-2) for 30 min ...

Emerging role of oncogenic β-catenin in exosome biogenesis as a driver of immune escape in hepatocellular carcinoma

Camille Dantzer, et al., bioRxiv - Cancer Biology 2024

Quote: ... Sections were rinsed with Dako Wash Buffer and antibodies were applied for 45 min according to the following dilutions (Dako Antibody Diluent): Glutamine synthetase (1:400 ...

Exposure to maternal obesity per se programs sex-differences in pancreatic islets of the offspring

L M Nicholas, et al., bioRxiv - Physiology 2019

Quote: ... Sections were incubated with primary antibody against insulin (Dako) and glucagon (Bioss Antibodies Inc) ...

Neuronal differentiation through GPI-anchor cleavage by GDE2: regulation via sequence-dependent trafficking

Fernando Salgado-Polo, et al., bioRxiv - Neuroscience 2019

Quote: ... Secondary antibodies conjugated to horseradish peroxidase (DAKO, Glostrup, Denmark) were incubated for 1 hr at room temperature ...

Apolipoprotein E isoform-specific phase transitions in the retinal pigment epithelium drive disease phenotypes in age-related macular degeneration

Nilsa La Cunza, et al., bioRxiv - Neuroscience 2020

Quote: ... and probed with antibodies to ApoE (1:300; Dako) and beta-tubulin (1:1000 ...

Olaparib and Enzalutamide synergistically suppress HCC progression via the AR-mediated miR-146a-5p/BRCA1 signaling

Jie Zhao, et al., bioRxiv - Synthetic Biology 2019

Quote: ... followed by incubation with HRP-conjugated secondary antibody (Dako) for 60 min at room temperature ...

Modulation of Actin network and Tau phosphorylation by HDAC6 ZnF UBP domain

Abhishek Ankur Balmik, et al., bioRxiv - Cell Biology 2019

Quote: ... and total Tau antibody K9JA (Dako, cat no A0024), pT181 (Invitrogen ...

Electrostatic interactions at the five-fold axis alter heparin-binding phenotype and drive EV-A71 virulence in mice

Han Kang Tee, et al., bioRxiv - Microbiology 2019

Quote: ... Isotype control antibodies or normal rabbit immunoglobulin fractions (Dako) were also used to exclude non-specific staining.

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