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Citations for Agilent :
1201 - 1250 of 6226 citations for Cow Protein L Isoaspartate PCMT1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... Sections were allowed to thaw at RT and thereafter blocked for > 60 min at RT with blocking-buffer (serum-free protein blocking solution, DAKO), supplemented with 0.2% Triton X-100 (Sigma Aldrich) ...
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bioRxiv - Biophysics 2019Quote: ... Major interfering proteins were removed from the serum by the Multiple Affinity Removal Spin Cartridge HSA/IgG (Agilent Technologies, USA) as described into manual ...
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bioRxiv - Bioengineering 2019Quote: ... The primary antibodies sources and dilutions were as follows: rabbit poly-clonal anti-glia fibrillary acidic protein (GFAP; 1:500; DAKO), mouse monoclonal anti-Neuronal Class III B-Tubulin (TuJ1 ...
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bioRxiv - Biophysics 2020Quote: ... bound to precursor protein was analysed by SDS-PAGE and liquid chromatography coupled to mass-spectrometry (LC ESI-TOF MS, 6210, Agilent Technologies ...
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bioRxiv - Biophysics 2020Quote: His-tagged CrSAS-6[NL] spanning amino acids 1-503 of the protein (see Supplementary Fig. 1a)1 was expressed in the Escherichia coli strain BL21(DE3) (Stratagene). Bacteria were grown at 37 °C in lysogeny broth (LB ...
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bioRxiv - Immunology 2021Quote: ... and by SEC-HPLC with an xbridge protein BEH SEC (Waters, ref. 186009160) connected to a HP1100 system (Agilent Technologies).
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bioRxiv - Molecular Biology 2022Quote: ... whole protein lysates for each well were quantified by BCA assay and total protein amounts were used for normalization of Seahorse data using Wave Software (Agilent). Basal OCR ...
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bioRxiv - Biochemistry 2022Quote: ... constructs containing DNA encoding 8X-His-tagged CHI-domain-containing proteins were transformed into BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent) for recombinant protein expression ...
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bioRxiv - Bioengineering 2022Quote: ... and incubated overnight at 4 °C with primary antibodies for the glial fibrillary acidic protein (GFAP; 1:1000, Z0334, Dako), ionised calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were probed with a set of 216 antibodies against total and phospho-proteins using an automated slide stainer Autolink 48 (Dako). Each slide was incubated with one specific primary antibody ...
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bioRxiv - Biochemistry 2024Quote: ... Fluorescence spectra of proteins containing ZnPPIX were measured from 550-750 nm in a Cary Eclipse Fluorescence Spectrophotometer (Agilent Technologies) with an excitation wavelength of 430 nm ...
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bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
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bioRxiv - Neuroscience 2024Quote: ... Every fourth section from approximately bregma −1.40 mm to −2.00 mm were immunostained with ionized calcium binding adaptor molecule 1 (Iba1; 1:2000; FUJIFILM Wako) and glial fibrillary acidic protein (GFAP; 1:1000; Dako); 4-6 sections per brain ...
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bioRxiv - Bioengineering 2024Quote: Intact protein mass spectrometry in positive ion mode was performed using a RapidFire 365 jet-stream electrospray ion source (Agilent) coupled to a 6550 Accurate-Mass Quadrupole Time-of-Flight (Q-TOF ...
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bioRxiv - Molecular Biology 2023Quote: The above 12 OR genes linked to a wild-type internal ribosome entry site from the encephalomyocarditis virus (EMCV IRES) and the mCherry fluorescent protein gene (Shaner et al., 2004) were inserted into a modified pBlueScript KS (Agilent/Stratagene) called plasmid “L” (Zboray et al. ...
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bioRxiv - Biochemistry 2023Quote: ... CLEANEX and DOSY data were collected on 100 μM 15N-labelled protein at a proton Larmour frequency of 600 (Agilent) or 800 (Bruker ...
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bioRxiv - Microbiology 2023Quote: Yeast proteins under both conditions were extracted and later subjected to fractionation through 3100 OFFGEL (Agilent Technologies, Palo Alto, CA) followed by an identification by LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Vectors encoding the recombinant proteins were constructed as described in the previous section and introduced into the BL21-CondonPlus (DE3)-RIL competent cells (Agilent Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were incubated overnight with the following primary antibodies: rabbit polyclonal anti-glial fibrillary acidic protein (GFAP) (1:6,000, DAKO, UK), rabbit polyclonal anti-allograft inflammatory factor 1 (IBA1 ...
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bioRxiv - Biochemistry 2023Quote: ... 10μL of purified protein was injected to a C-18 column (Agilent Zorbax 300SB-C18, 5 μM, 2.1 x 150mm) without dilution or buffer exchange ...
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bioRxiv - Immunology 2023Quote: ... Antigen retrieval was then performed for 20 minutes at 95°C using Lecia Epitope Retrieval Buffer 2 followed by treatment with Dako serum-free protein block (X090930-2, Agilent Dako) for 15 minutes to prevent non-specific binding of the antibody ...
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bioRxiv - Biochemistry 2023Quote: His6-SUMO-tagged and His6-SUMO-SNAP-tagged Tm1 protein constructs and GST-Khc 1- 365 were expressed in BL21-CodonPlus(DE3)-RIL cells (Stratagene) by isopropyl β-D-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2023Quote: The murine and human proteins (variants R166H and R168H, respectively) were produced in E.coli BL21 CodonPlus(DE3) RIL (Agilent Technologies) carrying the plasmid pHisTrx-mα-DGN and pHisTrx-hα-DGN ...
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bioRxiv - Biochemistry 2023Quote: ... Samples of trypsin-digested proteins were analyzed using the 1200 series LC system and LTQ-Orbitrap-XL mass spectrometer (Agilent and Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous peroxidases were blocked by incubation with 3 % H2O2 and then blocked for 1 h (Dako Serum-free protein block). Sections were then incubated with the 1st primary antibody (FABP7 ...
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bioRxiv - Cell Biology 2023Quote: ... For staining of macrophages and platelets the aortic tissue sections were blocked for 1 h at RT with protein blocking solution (#X0909, Dako). After blocking ...
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bioRxiv - Immunology 2023Quote: ... IgG from 100 µl of human plasma (∼100 µg/100 µl) was pre-enriched using the Protein G AssayMAP Bravo (Agilent) technology as described above ...
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bioRxiv - Molecular Biology 2024Quote: ... Individual protein fractions were next derivatized for subsequent analysis of deuterium enrichment in alanine using Gas Chromatography-Mass Spectroscopy (Agilent 7890B GC coupled to Agilent 5977B MS ...
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bioRxiv - Cell Biology 2024Quote: ... One aliquot was solubilized with 1% SDS to determine protein content and the other aliquot was mixed with 1 mL 30% HNO3 and subjected to ICP/MS 8900 (Agilent). Data was normalized by the corresponding protein concentration.
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bioRxiv - Cell Biology 2024Quote: ... Protein intact mass was determined using a 1969 MSD-ToF electrospray ionisation orthogonal time-of-flight mass spectrometer (Agilent Technologies). The instrument was configured with the standard ESI source and operated in positive ion mode ...
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bioRxiv - Neuroscience 2024Quote: ... Concentrated protein samples were then immunodepleted using a multi-affinity removal spin cartridge human-14 (0.45 mL, Agilent Technologies, UK) to remove high abundance proteins according to the manufacturer’s protocol with some modifications ...
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bioRxiv - Microbiology 2024Quote: ... human cGAS was expressed as a fusion protein with a 6×His-SUMO2 tag in Escherichia coli (E. coli) BL21-RIL DE3 (Agilent) bacteria harboring a pRARE2 tRNA plasmid ...
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bioRxiv - Molecular Biology 2024Quote: ... is a pCEP4-based plasmid that contains the humanized enhanced green fluorescent protein (hrGFP) coding sequence from phrGFP-C (Agilent) under the control of the pCEP4 CMV promoter and SV40 polyadenylation signal (39).
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were quantified by qPCR using the KAPA Library Quantification Kit for Illumina Libraries (KapaBiosystems; Cat#: KR0405) and library profiles were assessed using the DNA High Sensitivity HS Kit (Agilent; Cat#: 5067-4626) on an Agilent Bioanalyzer 2100 ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries fragment size distribution and concentration was assessed using Qubit dsDNA HS Assay Kit (Thermo Fischer Scientific, Q32851) and Agilent High Sensitivity DNA kit (Agilent Technologies, 5067-4626) on a bioanalyzer ...
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bioRxiv - Cancer Biology 2024Quote: ... PolyA+ stranded RNA library was prepared using the Illumina Stranded mRNA Prep kit and quality was assessed by using the 2100 Bioanalyzer (Agilent, DNA 7500 kit). RNA-seq libraries have been pooled equimolarly and sequenced using NovaSeq6000 (Illumina ...
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bioRxiv - Bioengineering 2021Quote: ... 10-20 µl injection volume depending on protein concentration) were injected on a Poroshell 300SB-C8 column (5 µm, 300Å, 1×75mm IDxL; Agilent Technologies) at a flow rate of 100 µl/min using solvent A (0.1% formic acid and 0.05% trifluoroacetic acid in water ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound antibody was removed with washing buffer and the fraction of cells with surface protein labeled with CD44 antibody was determined using a MoFlo cell sorter (Dako Cytomation).
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bioRxiv - Molecular Biology 2020Quote: ... All samples were depleted of the 7 most abundant proteins with the multiple affinity removal system (MARS Hu7 spin cartridge) according to the manufacturers protocol (Agilent Technologies). Samples were further reduced using 4 mM DTT ...
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bioRxiv - Microbiology 2019Quote: ... MBP-fusion proteins were purified with amylose resin following the manufacturer’s instructions (GE Healthcare, Pierce, Agilent Technologies and New England Biolabs). MBP-fusions (1 µg each in 100 µl of 1% NP40 ...
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bioRxiv - Cell Biology 2020Quote: Zyxin and Pxl1 LCR proteins were expressed in Escherichia coli strain BL21-Codon Plus (DE3)-RP (Agilent Technologies, Santa Clara, CA) with 0.5 mM isopropyl β-D-1-thiogalactopyranoside for 16 h at 16 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... approximately 8 µg of protein was injected on a Zorbax 300SB-C18 column (5 µm, 300Å, 1×250mm IDxL; Agilent Technologies) and separated using a 30 min gradient from 5% to 80% solvent B at a flow rate of 100 µl/min (solvent A ...
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bioRxiv - Immunology 2020Quote: ... followed by a quick rinse in TBS before being returned to the black chamber to be incubated with serum free protein block (Dako X0909) for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... brucei Heat Shock Protein 70 (TbHSP70) (Kind gift from Professor Keith Matthews, Edinburgh, UK) using a Dako Autostainer Link 48 (Dako, Denmark) and were subsequently counterstained with Gill’s haematoxylin.
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bioRxiv - Biochemistry 2022Quote: ... Proteins were detected using tryptophan fluorescence (λex=280 nm; λem=315 nm) using a fluorescence detector (Agilent technologies 1200 series, G1321A). The peak heights of the monomeric hsFPN peaks were used to assess the stability by normalizing heights to the corresponding value from samples incubated at 4 °C (100% stability) ...
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bioRxiv - Cancer Biology 2020Quote: ... the serum samples were immunoaffinity depleted of the 14 most abundant proteins using IgY14 LC20 (cat No. 5188-6557, Agilent, USA). The depletion was performed on an Agilent 1260 HPLC (1260 Infinity ...
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bioRxiv - Biophysics 2019Quote: ... The intact protein mass was detected by LC/MS on an Open Access MS system (Agilent 1290 UHPLC + Agilent 6530 QToF) and analyzed by the MassHunter software.
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bioRxiv - Biophysics 2019Quote: ... The intact protein mass was detected by LC/MS on an Open Access MS system (Agilent 1290 UHPLC + Agilent 6530 QToF) and analyzed by the MassHunter software.
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bioRxiv - Biochemistry 2021Quote: Serum samples were depleted of the top 14 interfering high-abundant proteins using the Multiple Affinity Removal Column Human 14 (Agilent, USA). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies were directed against: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), epithelial membrane antigen (EMA ...